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12.2 DNA Replication ppt
12.2 DNA Replication ppt

... must be replicated exactly  All somatic cells that undergo mitosis get a new strand of DNA and an “old” one  This occurs in the S phase of interphase  Cannot be accomplished without the aid of enzymes. ...
The History of DNA
The History of DNA

... ...
Nucleic Acids - faculty at Chemeketa
Nucleic Acids - faculty at Chemeketa

... What will be the composition of the DNA strand complementary to –AGCCA– ? a. b. c. d. ...
Molecular genetics of gene expression
Molecular genetics of gene expression

... 5’ to 3’ and when paired strands are in “antiparallel” orientation ...
Mutations & Genetic Engineering
Mutations & Genetic Engineering

... –Huntington’s disease • Inversion – orientation is in the reverse direction • Translocation – two non homologous chromosomes exchange segments ...
Heredity Picture Vocabulary
Heredity Picture Vocabulary

... the genetic information needed to make new cells and carry out cell functions. ...
Recombinant DNA - Westwind Alternate School
Recombinant DNA - Westwind Alternate School

Jeopardy - Grayslake Central High School
Jeopardy - Grayslake Central High School

... the process of PCR? It denatures the DNA which means that the hydrogen bonds holding together complementary bases of opposite strands break. This separates the strands. ...
Use the diagram to match the letter (A-C) to the correct term(1
Use the diagram to match the letter (A-C) to the correct term(1

... 8. In DNA, which of the following determines the traits of an organism? a. Amount of adenine b. Number of sugars c. Sequence of nitrogen bases d. Strength of hydrogen bonds 9. You have separated the nucleotides in a piece of DNA. You find that 22% of the bases are adenine nucleotides. What percentag ...
jeopardy honors DNA 12-1 thru 12-4 only
jeopardy honors DNA 12-1 thru 12-4 only

... strand; and therefore, the new DNA consists of only one newly synthesized strand per double ...
REPLICATION, TRANSCRIPTION, TRANSLATION TAKS
REPLICATION, TRANSCRIPTION, TRANSLATION TAKS

... 14 Part of a DNA strand is represented in the diagram above. In order for DNA to replicate, the strand must separate at which of the following locations? F Between every phosphate-sugar pair G Between the eight sugar-base pairs H* Between the four nitrogenous base pairs J Between any two chemical bo ...
Molecular Cloning
Molecular Cloning

... National Dong Hwa University ...
IntrotoBiotechRestrictionEnzymes2011
IntrotoBiotechRestrictionEnzymes2011

... • some restriction enzymes (like EcoRI) produce cuts in the DNA that result in the formation of sticky ends on the DNA fragments that are formed. • sticky ends indicates that unpaired bases are left hanging off the cut. other restriction enzymes produce blunt ends, that is, the DNA is cut directly ...
DNA Structure and Function Vocabulary
DNA Structure and Function Vocabulary

... form all of an organism’s enzymes and structural proteins. ...
Molecular Basis of Inheritance
Molecular Basis of Inheritance

... Categorizing the Role • The function of DNA was first described from tests utilizing microbes and viruses • Frederick Griffith (1928) • First to describe Transformation: change in genetic and physical composition as a result of assimilation of external DNA ...
Biotechnology Unit Test Review
Biotechnology Unit Test Review

... 1. Recombinant DNA – sequence of DNA made from two or more species (in class, we combined a human gene with a bacterial DNA plasmid) 2. Restriction enzymes – Bacterial enzymes that cut DNA in specific places (look for specific nitrogen base sequences and make zig-zag cut to make “sticky ends”) 3. Ge ...
DNA - VanityWolveriine
DNA - VanityWolveriine

... molecules, or base pairs, called nucleotides: adenine (A), thymine (T), guanine (G), and cytosine (C) that are linked end to end. Each base on the opposite strand specifically pairs with, or is the complement of, the other: an A always pairs with a T, and a C always pairs with a G. A DNA molecule wi ...
Microbiology Unit 3 Study Guide
Microbiology Unit 3 Study Guide

... 5. Which enzyme makes RNA by reading a strand of DNA? 6. Which enzymes cut DNA in specific locations? 7. What occurs during transcription? 8. What are the steps to obtaining DNA fragments for gel electrophoresis? 9. Which enzyme reads DNA to make a new copy of DNA? 10. How has Escherichia coli been ...
Document
Document

... DNA Forensics and Civil Liberties Workshop Summary •Perspective on DNA Testing & Forensics - Rothstein •Daubert Standard •Listen to the Experts -- Daubert, Frye, and California ...
Hierarchy of Genetics
Hierarchy of Genetics

... -codes for Genes ...
Cloning
Cloning

... Contain an oriV that allows for high copy number, may have narrow (pUC) or broad (R) host ranges Small – why is this an advantage? Selectable Genes Unique restriction sites May have additional features such as mob sites, RNA polymerase promoters, etc. ...
DNA!
DNA!

... Some disorders that can be tested using DNA screening are: 1. Down Syndrome – tested using an amniocentesis which allows a lab tech to create a karyotype (a map of chromosomes) to determine if trisomy 21 (3 chromosomes at 21 instead of 2) occurs. 2. PKU – tested using a blood sample to look for the ...
genetics science learning center – internet lesson
genetics science learning center – internet lesson

... Click on the link that says “Tour the basics”. A new window will open, you can navigate sections by using the top toolbar. WHAT IS DNA? 1. What does DNA stand for? 2. Why is DNA called a blueprint? 3. The “twisted ladder” shape of the DNA molecule is called a _____________________ 4. Name the four b ...
Genetic Engineering
Genetic Engineering

... allows us to change the characteristics of a cell or cells. ...
What Is Gene cloning and How Is It Used? 1. Explain what is meant
What Is Gene cloning and How Is It Used? 1. Explain what is meant

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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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