GENETIC ENGINEERING AND BIOTECHNOLOGY Course code
... Definition of genetic engineering and biotechnology, and how have they emerged from other sciences. A historical perspective of biotechnology, and the key events in the evolution of biotechnology. Cloning, expression and analysis of genes and their products (7 hours) Concept of molecular cloning, pl ...
... Definition of genetic engineering and biotechnology, and how have they emerged from other sciences. A historical perspective of biotechnology, and the key events in the evolution of biotechnology. Cloning, expression and analysis of genes and their products (7 hours) Concept of molecular cloning, pl ...
Process of Electrophoresis
... Agarose gel electrophoresis is a procedure used to separate DNA fragments based on their sizes. DNA is an acid and has many negative electrical charges. Scientists have used this fact to design a method that can be used to separate pieces of DNA. A solution containing a mixture of DNA fragments of v ...
... Agarose gel electrophoresis is a procedure used to separate DNA fragments based on their sizes. DNA is an acid and has many negative electrical charges. Scientists have used this fact to design a method that can be used to separate pieces of DNA. A solution containing a mixture of DNA fragments of v ...
Protein Synthesis Simulation
... DNA carries the information for the synthesis of all the proteins of an organism. Protein molecules are large and complex, composed of hundreds of amino acids. The sequence of amino acids in a protein molecule is determined by the sequence of the nucleotides in the DNA of an organism. In the first s ...
... DNA carries the information for the synthesis of all the proteins of an organism. Protein molecules are large and complex, composed of hundreds of amino acids. The sequence of amino acids in a protein molecule is determined by the sequence of the nucleotides in the DNA of an organism. In the first s ...
Pathogen induced genome instability
... bacterial reproduction, and in addition to mutation, are another major source of genetic variation in bacteria. ...
... bacterial reproduction, and in addition to mutation, are another major source of genetic variation in bacteria. ...
Scientific Writing
... Plasmids are easily manufactured in large amounts DNA is very stable DNA resists temperature extremes and so storage and transport are straight forward A DNA sequence can be changed easily in the laboratory can respond to changes in the infectious agent By using the plasmid in the vaccin ...
... Plasmids are easily manufactured in large amounts DNA is very stable DNA resists temperature extremes and so storage and transport are straight forward A DNA sequence can be changed easily in the laboratory can respond to changes in the infectious agent By using the plasmid in the vaccin ...
notes
... mRNA can now leave the nucleus (through nuclear pore) and enter the cytoplasm cut out introns and splice exons together ...
... mRNA can now leave the nucleus (through nuclear pore) and enter the cytoplasm cut out introns and splice exons together ...
Key for Exam 1 Part 1 - Evolutionary Biology
... intermediate flower color are aborted within the seed pod and thus never develop (B) The seeds coding for intermediate flower color have deleterious alleles that prevent them from germinating (C) These variations in human are affected by lack of dominance in the alleles that control these traits (D) ...
... intermediate flower color are aborted within the seed pod and thus never develop (B) The seeds coding for intermediate flower color have deleterious alleles that prevent them from germinating (C) These variations in human are affected by lack of dominance in the alleles that control these traits (D) ...
PCR labwork 2 ENG
... Student name, surname_____________________________________________________ ...
... Student name, surname_____________________________________________________ ...
BIO 311C Introductory Biology Student Learning Outcomes
... 13. Gene Regulation: Cells can regulate gene expression at many points during the process. A. Using a model system example, tell how prokaryotic genomes are structured to efficiently regulate initiation of t ...
... 13. Gene Regulation: Cells can regulate gene expression at many points during the process. A. Using a model system example, tell how prokaryotic genomes are structured to efficiently regulate initiation of t ...
Molecular genetics of bacteria
... • Many genes in prokaryotes are grouped together in the DNA and are regulated as a unit. Genes are usually for enzymes that function together in the same pathway. • At the upstream end are sections of DNA that do not code, but rather are binding sites for proteins involved in regulation (turning gen ...
... • Many genes in prokaryotes are grouped together in the DNA and are regulated as a unit. Genes are usually for enzymes that function together in the same pathway. • At the upstream end are sections of DNA that do not code, but rather are binding sites for proteins involved in regulation (turning gen ...
doc - Florida State University
... 36. Retroviruses differ from bacteriophages in that they ______________. (A) Infect bacterial cells only, (B) contain both DNA and RNA as their genetic information, (C) do not produce diseases in humans, (D) contain RNA as their genetic information OR (E) lack protein in their capsids. 37. Consider ...
... 36. Retroviruses differ from bacteriophages in that they ______________. (A) Infect bacterial cells only, (B) contain both DNA and RNA as their genetic information, (C) do not produce diseases in humans, (D) contain RNA as their genetic information OR (E) lack protein in their capsids. 37. Consider ...
Pierce5e_ch19_lecturePPT
... Cloning Genes • Plasmid vectors – Plasmids: circular DNA molecules from bacteria – Insert foreign DNA into plasmid using restriction enzymes – Linkers: synthetic DNA fragments containing restriction sites ...
... Cloning Genes • Plasmid vectors – Plasmids: circular DNA molecules from bacteria – Insert foreign DNA into plasmid using restriction enzymes – Linkers: synthetic DNA fragments containing restriction sites ...
2014
... A) δ-aminolevulinate is a tetrapyrrole precursor. B) Carbon atoms of tetrapyrroles are derived from glycine and succinyl-CoA C) Carbon atoms of tetrapyrroles are derived from glutamate. D) A total of 4 δ-aminolevulinate molecules are used to synthesize 1 heme molecule. Circle the correct answer. 2. ...
... A) δ-aminolevulinate is a tetrapyrrole precursor. B) Carbon atoms of tetrapyrroles are derived from glycine and succinyl-CoA C) Carbon atoms of tetrapyrroles are derived from glutamate. D) A total of 4 δ-aminolevulinate molecules are used to synthesize 1 heme molecule. Circle the correct answer. 2. ...
doc - Florida State University
... 38. When the ___________ binds to the enhancer sites in a eukaryotic gene, the DNA folds in such a way that it comes in close proximity to the ____________. (A) RNA polymerase II, promoter region, (B) mRNA, activator region, (C) activator proteins, termination site, (D) start codon, stop codon OR (E ...
... 38. When the ___________ binds to the enhancer sites in a eukaryotic gene, the DNA folds in such a way that it comes in close proximity to the ____________. (A) RNA polymerase II, promoter region, (B) mRNA, activator region, (C) activator proteins, termination site, (D) start codon, stop codon OR (E ...
ppt
... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
Worksheet 1 (isolation)
... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
Exam MOL3000 Introduction to Molecular Medicine
... a) Which are the most common types of damage caused by UV-light? Define them by name and molecular structure. b) Which DNA repair mechanism is responsible for repair of the most common type of UV-light damage? c) Which type of damage to DNA is caused by N-nitrosocompounds? Give at least one example ...
... a) Which are the most common types of damage caused by UV-light? Define them by name and molecular structure. b) Which DNA repair mechanism is responsible for repair of the most common type of UV-light damage? c) Which type of damage to DNA is caused by N-nitrosocompounds? Give at least one example ...
Chapter 13: Genetic Technology
... 3. Separating DNA Gel Electrophoresis – a technique for separating DNA DNA is negatively charged Load DNA into gel Apply electric voltage to gel DNA will move through gel to positive Smaller DNA fragments move faster and further How do you end up with different size fragments that are ...
... 3. Separating DNA Gel Electrophoresis – a technique for separating DNA DNA is negatively charged Load DNA into gel Apply electric voltage to gel DNA will move through gel to positive Smaller DNA fragments move faster and further How do you end up with different size fragments that are ...
Biology
... Livestock given genes for extra growth hormone Poultry given genes to resist infection ...
... Livestock given genes for extra growth hormone Poultry given genes to resist infection ...
1 - cellbiochem.ca
... PRESENCE OF A RE BINDING SITE ON A PIECE OF DNA IMMEDIATELY TELLS YOU THAT THIS SITE WILL BE CLEAVED IN THE PRESENCE OF THIS RE. Cut palindromic sequences Generate either blunt or protruding ends. ...
... PRESENCE OF A RE BINDING SITE ON A PIECE OF DNA IMMEDIATELY TELLS YOU THAT THIS SITE WILL BE CLEAVED IN THE PRESENCE OF THIS RE. Cut palindromic sequences Generate either blunt or protruding ends. ...
Chapter 20: Biotechnology 11/18/2015
... Gene cloning usually involves the use of restriction enzymes that cut DNA at very specific sequences: ...
... Gene cloning usually involves the use of restriction enzymes that cut DNA at very specific sequences: ...
Genetic engineering
... • How do scientists figure out the nucleotide sequence of a gene? (That is, how do they know the order of the C, G, T, and A?) • Sanger method was developed first – click here for animation 1) DNA is heated so that it separates 2) A primer is added to get DNA synthesis started 3) Nucleotides are add ...
... • How do scientists figure out the nucleotide sequence of a gene? (That is, how do they know the order of the C, G, T, and A?) • Sanger method was developed first – click here for animation 1) DNA is heated so that it separates 2) A primer is added to get DNA synthesis started 3) Nucleotides are add ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.