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TUTORIAL FIGURES: Basic Molecular Biology
TUTORIAL FIGURES: Basic Molecular Biology

... Figure 3: Information transfer processes: the central dogma of molecular biology. Depicted are three information transfer processes: (1) replication when DNA duplicates, (2) transcription when DNA copied to RNA ¥ ¥ ¥ ¥ ), and (3) translation when the RNA message is translated to make protein. Micro ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... Always has the same volume DNA is much denser than protein More DNA in phage, denser phage Extra DNAs that can inactivate a gene by inserting into it were the first transposons discovered in bacteria • These transposons are called insertion sequences (ISs) ...
m5zn_7de32f5a588b6c7
m5zn_7de32f5a588b6c7

... DNA replication must occur before a cell can divide; in cancer, drugs with molecules similar to the four nucleotides are used to stop replication. ...
File
File

... For example, the mRNA complement to the DNA sequence TTGCAC is AACGUG. The SAT II Biology frequently asks about the sequence of mRNA that will be produced from a given sequence of DNA. For these questions, don’t forget that RNA uses uracil in place of thymine. After transcription, the new RNA strand ...
PowerPoint Presentation Materials to accompany
PowerPoint Presentation Materials to accompany

... It is also possible for any other DNA fragment to be inserted into the plasmid And it is possible for the plasmid to circularize without an insert This is called a recircularized vector ...
GDP-HiFi DNA Polymerase
GDP-HiFi DNA Polymerase

... GDP-HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). GDP-HiFi has higher stability at high temperature. Users may program the init ...
File
File

... Splicing of mRNA increases the number of different proteins an organism can produce. Gene expression is regulated by proteins that bind to specific base sequences in DNA. The environment of a cell and of an organism has an impact on gene expression. The promoter as an example of non-coding DNA with ...
Exam 2
Exam 2

... shown that chi structures generally have two pairs of equal length arms (as shown below). Explain why chi structures exhibit this particular symmetry. Homologous recombination is an exchange of DNA between similar or identical molecules of DNA, such as homologous chromosomes. This symmetry arises be ...
Transcription Protein Synthesis So what does it mean? Transcription
Transcription Protein Synthesis So what does it mean? Transcription

... genetic code for protein synthesis • Proteins are the structural building blocks for cells, and they act as enzymes • Geneticists accept that the basic mechanism of reading and expressing genes is DNA  RNA  protein. • This chain of events occurs in all living things, from bacteria to humans. • Sci ...
5.2.3 Genomes and Gene Technologies
5.2.3 Genomes and Gene Technologies

The Molecular - MolGen | RuG
The Molecular - MolGen | RuG

... the laboratory of biochemist Erwin Chargaff. It was already known that DNA is a polymer of nucleotides, each consisting of three components: a nitrogenous (nitrogen-containing) base, a pentose sugar called deoxyribose, and a phosphate group (Figure 16.5). The base can be adenine (A), thymine (T), gu ...
Toxicity of benzo[a]pyrene occurs because of the formation of
Toxicity of benzo[a]pyrene occurs because of the formation of

... Toxicity of benzo[a]pyrene occurs because of the formation of covalent adducts with DNA guanines. In this work we report the attempt to detect this DNA-adduct using both an electrochemical assay based on gold nanoparticles and a surface plasmon resonance DNA sensor. Detection was achieved via inhibi ...
point mutation
point mutation

... Sulfur-35 was used to label the protein coat of the virus. Phosphorus-32 was used to label the phosphate backbone of DNA. When future generations were checked, in the sulfur experiment there was no sign of the radioactive isotope. In the phosphorus experiment, the new generations were still radioact ...
File
File

... • Process of removing DNA from one organism and putting it into another. • EX: the gene for insulin is removed from a pig or human and inserted into E.coli bacteria. The E. coli then produce the insulin that is purified , bottled, and distributed to people in need. The insulin was “cloned” ...
PCRBIO Taq DNA Polymerase
PCRBIO Taq DNA Polymerase

GENE EXPRESSION - PROTEIN SYNTHESIS A. FROM DNA TO
GENE EXPRESSION - PROTEIN SYNTHESIS A. FROM DNA TO

... parents produce sperm and egg which fuse to form a zygote, the first cell of a new individual. Of course, sexual reproduction does not occur in bacteria, but even they have mechanisms of genetic transfer. Gene transfer is significant because it greatly increases the genetic diversity of organisms. W ...
Recombinant DNA and the Production of Insulin
Recombinant DNA and the Production of Insulin

... Recombinant DNA and the Production of Insulin Part One Diabetes is a condition where a person has too much sugar in their blood. Insulin, which is a hormone created by the pancreas, normally helps lower the level of sugar in a person’s blood. But people who are diabetics do not produce enough insuli ...
Stable Nuclear Transformation of the diatom Phaeodactylum
Stable Nuclear Transformation of the diatom Phaeodactylum

... The ability to insert a selectable marker into a diatom enables the manipulation of the gene structure and the regulation of the genome.  With the ability to perform a nuclear transformation, a new door is open that allows diatoms to be mass produced in a laboratory making it more accessible for bo ...
Materials and methods (Supplement)
Materials and methods (Supplement)

... with 1 bp staggered cuts are generated and formed hairpin structures at the position adjacent to the transposable element. These hairpin structures are resolved by nicks and both open ends of genomic DNA are ligated to create various inversions or direct repeats which depend on the position of nicks ...
Sequences vs Viruses: Producer vs Product, Cause and
Sequences vs Viruses: Producer vs Product, Cause and

... It was shown that the correct chromosome number for humans was 46 (not 48), that the genetic code was arranged in triplets, that restriction enzymes could be used to cut and paste sections of DNA, and then, in 1983, Kary Mullis devised the extraordinary polymerase chain reaction, enabling relative e ...
DNA and Protein Synthesis ppt outline notes 07
DNA and Protein Synthesis ppt outline notes 07

... Scientists use their knowledge of the structure of DNA and its chemical properties to study and change DNA molecules. Scientists use different techniques to: from cells into smaller pieces the sequence of ...
DNA: the Molecule of Heredity
DNA: the Molecule of Heredity

... • T. H. Morgan’s group showed that genes are located on chromosomes, the two components of chromosomes— DNA and protein—became candidates for the genetic material • The discovery of the genetic role of DNA began with research by Frederick Griffith in 1928 who worked with two strains of a bacterium, ...
No Slide Title
No Slide Title

... DNA metabolism Replication Early on - “Template” so molecules can line up in a specific order and be joined to create a new macromolecule 1940s - DNA = genetic material 1950s - structure identified how it could act as a template for replication and transmission of genetic info One strand is the comp ...
UNIT 5 - UtechDMD2015
UNIT 5 - UtechDMD2015

... Because of their restriction site specificity, the restriction endonucleases cut DNA into fragments whose average length is determined by the number of base pairs in the restriction site (and to a lesser extent by the ratio of bases in the DNA). For DNA that has equal amounts of all four bases, each ...
DNA metabolism
DNA metabolism

... Recombination - linear sequence of DNA altered by cleavage and rejoining of chromosome (involves RecA protein) Repair of this type sometimes needed to reconstruct replication fork Human breast cancer genes (BRCA1 and BRCA2) produce proteins that interact with the human homolog of RecA, therefore the ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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