• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Protocol S1
Protocol S1

... both 98HAH12 (a human isolate from Haian County, Jiangsu Province, 1998) and 05ZYH33 (a human isolate in Ziyang County, Sichuan Province, 2005) were representative of highly pathogenic SS2 isolated from fatal cases of STSS[1]. 05HAS68 (a healthy swine isolate in Haian County, Jiangsu Province, 2005) ...
Transposons
Transposons

... Mu integrates by transposition replicates when E. coli replicates During the lysogenic cycle, Mu remains integrated in E. coli chromosome ...
DNA - smoser
DNA - smoser

... The new DNA strand is shorter than the template strand. As a result of the inability of DNA polymerase to initiate synthesis, the DNA molecule becomes shorter with each cell division. Human chromosomes have the sequence "TTAGGG" repeated 100 to 1500 times at each end of the DNA strand. These repetit ...
document
document

... deletion of 4 nucleotides corresponding to base numbers 4184-4187 of SEQ ID NO:1 ...
DETERMINATIVE DEGREE AND NUCLEOTIDE CONTENT OF DNA
DETERMINATIVE DEGREE AND NUCLEOTIDE CONTENT OF DNA

... The trinucleotide DNA can be listed in the similar and more cumbersome way. The full DNA sequences consist of nucleotides of all four types and are described by (10)-(11). The introduction of the determinative degree allows us to single out a kind of double-helix DNA sequences which have an addition ...
protein synthesis worksheet
protein synthesis worksheet

... Circle the correct choice within the parenthesis for 1 -18. 1. (DNA/RNA) can leave the nucleus. 2. mRNA is made during (transcription/translation). 3. mRNA is made in the (cytoplasm/nucleus). 4. DNA is located in the (nucleus/cytoplasm) 5. (Translation/Transcription) converts DNA into mRNA. 6. (mRN ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

... The contribution of computers (or perhaps more appropriately, computer science) is text processing. Since DNA sequences come as a sequence of characters (from the alphabet A, C, T, G), there are several computer science algorithms that can be used as processing tools. The field of phylogenetics has ...
Mutation and Genetic Variation - Cal State LA
Mutation and Genetic Variation - Cal State LA

... ... were able to “choose which mutations will occur” by directing mutation towards the broken lac gene - from 1988-1994, at least 5 papers argued for “directed mutation” when cells were grown on a nutrient they couldn’t use This directly contradicts the fundamental premise of Darwinian evolution by ...
Chapter 11 Radiation Damage to Biomolecules — From water
Chapter 11 Radiation Damage to Biomolecules — From water

... S may have one of two values, ±1/2. Thus, there are two possible energy states. This is the key to understanding the EPR technique. Free radicals in a magnetic field are divided into two groups (the magnetic moments either oppose B or align with B) each group having a different energy. When the samp ...
documentation dates
documentation dates

... Define biotechnology and give some examples of how biotechnology can be used to improve human health. ...
QIAxcel® system — linkage analysis of zebrafish mutants
QIAxcel® system — linkage analysis of zebrafish mutants

Midterm #1 Study Guide
Midterm #1 Study Guide

... What is the difference between mitosis and meiosis? Where do these processes occur? What are the results from each? Proteins associated with DNA in eukaryotes are called ______. Histone–DNA units are called _______. Chromatids that are attached at the centromere are called what kind of chromatids? ...
Final Exam answer key
Final Exam answer key

Prof. Kamakaka`s Lecture 15 Notes
Prof. Kamakaka`s Lecture 15 Notes

... There is no phylogenetic relationship to DNA content There are sibling amphibian species - they look morphologically identical but have 4-fold difference in DNA content ...
Lab 1 Artificial Selection The purpose of a particular investigation
Lab 1 Artificial Selection The purpose of a particular investigation

... The roots of several onion bulbs were removed. Then the remaining severed root mass of onions bulbs were submerged in a beaker of distilled water, and others were submerged in a beaker of water seeped with weeping willow bark. It has been reported that a substance found in the bark of the weeping wi ...
SEQUENCE
SEQUENCE

...  http://www.ncbi.nlm.nih.gov/Entrez/ ...


... 18. Which of the following conditions would cause the release of the Lac repressor protein from the lac operator site on DNA? a) Presence of glucose in the growth media. b) Presence of arabinose in the growth media. c) Presence of IPTG in the growth media. d) Presence of ribose in the growth media. ...
POLYMERASE-CHAIN-REACTION (PCR) ANALYSIS OF
POLYMERASE-CHAIN-REACTION (PCR) ANALYSIS OF

... repeat units (Luty et al. 1990, Riesset al. 1990), was also present in either of the parents; for a situationsupportedby the factthat extrabands homozygousoffspring,the allele was found in occur with multiples of the length of the repeat both parents (Fig. 2). Data were consistentwith unit. The poss ...
No Slide Title
No Slide Title

... § Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions. § Commercial kits are now available for easy PCR reaction setup and amplification. § Contaminant DNA, such as from fungal and bacterial sources, will not amplify because human-specific p ...
The role of different positively and negatively charged ions on the
The role of different positively and negatively charged ions on the

... superhelix (147 base pair long sequence) is wrapped around the nucleosome core built by eight histone proteins. A detailed structural investigation (Davey et al., 2002) has shown that there are over 120 direct protein–DNA interactions as salt bridges between the main chain amides of the histone and ...
Leukaemia Section t(10;11)(p11.2;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(10;11)(p11.2;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... A. Partial Q-banded karyotype showing the t(10;11)(p11.2;q23), derivative chromosomes are on the right. B. FISH using RP13-31H8 (ABI1) shows one signal on the normal chromosome 10 and the another one split between the p arm of der(10) (arrowheads) and the q arm of der(11) (arrow). The BAC clone was ...
DNA measurements in low volume samples
DNA measurements in low volume samples

... (A977-A900) 1cm (Water/Buffer*) ...
Cloning The Law, Ethics and Sciences
Cloning The Law, Ethics and Sciences

... the ability to clone healthy human organs. Scientists have stated that cloning can be used to clone a healthy organ of some sort and replace the damaged with the new healthy one. A specific example is that of a heart - by being able to treat heart attack victims by cloning their healthy heart cells ...
Preparation and analysis of environmental DNA: optimisation of
Preparation and analysis of environmental DNA: optimisation of

... are important as subsequent analysis by polymerase chain reaction (PCR) may be problematic with sheared DNA or may not occur at all in the presence of certain impurities. Any loss of template, interference with amplification of recovered template or bias for highly concentrated template over rare te ...
Fill in blank notes - Cathkin High School
Fill in blank notes - Cathkin High School

... the origins of related species. Personalised medicine is based on an individual’s genome. By understanding the link between a person’s genes and certain diseases could lead to personalised medicine. For example, it may be that individuals carrying a particular allele of a gene have an _____________ ...
< 1 ... 181 182 183 184 185 186 187 188 189 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report