Lecture 11 - Horizontal Gene Transfer S11 2 slides per page
... Example - Streptococcus pneumoniae (GPC) •Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus influenzae (GNR) •Cell binds DNA only from related species Artificial competence In ...
... Example - Streptococcus pneumoniae (GPC) •Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus influenzae (GNR) •Cell binds DNA only from related species Artificial competence In ...
Lecture 11 - Horizontal Gene Transfer Chapt. 8 S11
... Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus influenzae (GNR) •Cell binds DNA only from related species Artificial competence In the laboratory, treat cells with specific ...
... Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus influenzae (GNR) •Cell binds DNA only from related species Artificial competence In the laboratory, treat cells with specific ...
... Cells from this plant have been taken for examination. Hurry tells why he chose cells from this plant. - They have large, easy to see chromosomes. Film sequence showing process of mitosis. The different stages of mitosis are pointed out and explained by S. Hurry. Film is stopped at each stage and st ...
What are prokaryotes?
... Thin layer peptidoglycan / outer membrane present HARDER to Kill!!! Stains Pink ...
... Thin layer peptidoglycan / outer membrane present HARDER to Kill!!! Stains Pink ...
Semester Exam Review
... Relationship of monomers to polymers. Give examples Difference between RNA and DNA What is the difference between active and passive transport? Give examples ...
... Relationship of monomers to polymers. Give examples Difference between RNA and DNA What is the difference between active and passive transport? Give examples ...
Spring 2007 - Antelope Valley College
... DNA synthesis in the direction that goes away from the replication fork is called ____________________________ strand synthesis. ...
... DNA synthesis in the direction that goes away from the replication fork is called ____________________________ strand synthesis. ...
Biotechnology
... • Are GMO’s safe for people and the environment? • Does embryonic stem cell research kill babies or simply use human tissue for the good of mankind? • Should people be allowed to choose the trait of their child? ...
... • Are GMO’s safe for people and the environment? • Does embryonic stem cell research kill babies or simply use human tissue for the good of mankind? • Should people be allowed to choose the trait of their child? ...
Genetic Improvement of Crop Plants short version with animation links
... genotype often masked by environmental influences. For specific characteristics such as fatty acid % of oil, naturally occurring mutants screened for and if not found induced by chemical mutagenesis or irradiation. ...
... genotype often masked by environmental influences. For specific characteristics such as fatty acid % of oil, naturally occurring mutants screened for and if not found induced by chemical mutagenesis or irradiation. ...
Molecular characterization of individual DNA double strand breaks
... While these techniques can be used to identify DSBs they provide no information on the molecular events occurring at the break. Tip Enhancement Raman Scattering (TERS) can provide molecular information from DNA at the nano-scale and in combination with AFM provides a new way to visualize and charact ...
... While these techniques can be used to identify DSBs they provide no information on the molecular events occurring at the break. Tip Enhancement Raman Scattering (TERS) can provide molecular information from DNA at the nano-scale and in combination with AFM provides a new way to visualize and charact ...
DNA REVIEW SHEET (answer in COMPLETE sentences on another
... Describe the function of each enzyme associated with DNA: helicase, DNA polymerase, DNA ligase, topoisomerase, editase). Explain in detail how DNA replicates. Completely discuss how transcription works, elaborating on how DNA strand, mRNA, and RNA polymerase are involved. (if you need additional hel ...
... Describe the function of each enzyme associated with DNA: helicase, DNA polymerase, DNA ligase, topoisomerase, editase). Explain in detail how DNA replicates. Completely discuss how transcription works, elaborating on how DNA strand, mRNA, and RNA polymerase are involved. (if you need additional hel ...
CONFOUNDING PHYLOGENETIC TREES
... -according to rRNA based phylogenies, there are 3 kingdoms of life – bacteria, archaea and eukarya with eukarya derived from archaea -the sequencing of hundreds of genomes has called into question this tree because many proteins in any one organism can be archaeal or bacterial in origin -gene swappi ...
... -according to rRNA based phylogenies, there are 3 kingdoms of life – bacteria, archaea and eukarya with eukarya derived from archaea -the sequencing of hundreds of genomes has called into question this tree because many proteins in any one organism can be archaeal or bacterial in origin -gene swappi ...
Agriscience Applications
... – Components of cells that determine individual characteristics of all living things – Comprised of DNA • The “Blueprint” of cells and their successive cells ...
... – Components of cells that determine individual characteristics of all living things – Comprised of DNA • The “Blueprint” of cells and their successive cells ...
Trends in Biotechnology
... Mammalian Cell Vectors. There are several: Simian virus 40 (SV40) — a small DNA tumor virus, could only hold a small piece of DNA and caused only transient (temporary) expression of the inserted DNA. Retrovirus — a single-stranded RNA virus that contains a gene for the enzyme reverse transcriptase ...
... Mammalian Cell Vectors. There are several: Simian virus 40 (SV40) — a small DNA tumor virus, could only hold a small piece of DNA and caused only transient (temporary) expression of the inserted DNA. Retrovirus — a single-stranded RNA virus that contains a gene for the enzyme reverse transcriptase ...
DNA_Technology_part2
... • The plasmids must be reintroduced into the host cell e.g. bacteria • This process is called transformation. • The bacteria, plasmids and calcium are mixed together. • By altering the temperature the bacteria become permeable and the plasmid can pass through the cell membrane. ...
... • The plasmids must be reintroduced into the host cell e.g. bacteria • This process is called transformation. • The bacteria, plasmids and calcium are mixed together. • By altering the temperature the bacteria become permeable and the plasmid can pass through the cell membrane. ...
second of four for Chapter 9
... • In Hfr transfer, some of the transferred DNA fragment gets incorporated into the recipient chromosome. • This will result in the F- cell becoming recombinant, but the Hfr cell stays the same. ...
... • In Hfr transfer, some of the transferred DNA fragment gets incorporated into the recipient chromosome. • This will result in the F- cell becoming recombinant, but the Hfr cell stays the same. ...
Biotechnology Part 3 Outline
... A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These enzymes create restriction f ...
... A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These enzymes create restriction f ...
6 Day 9 Biotechnology Part 3 Outline
... A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These enzymes create restriction f ...
... A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These enzymes create restriction f ...
Chapter 21
... 3’ to 5’ direction lagging strand. 5’ to 3’ direction is the leading strand. Okazaki fragments are made on the lagging strand. DNA returns to a coiled structure. Two identical DNA strands are made. • Takes about 8 hours to complete 3 billion base pairs. • https://www.youtube.com/watch?v=dKubyIRiN84 ...
... 3’ to 5’ direction lagging strand. 5’ to 3’ direction is the leading strand. Okazaki fragments are made on the lagging strand. DNA returns to a coiled structure. Two identical DNA strands are made. • Takes about 8 hours to complete 3 billion base pairs. • https://www.youtube.com/watch?v=dKubyIRiN84 ...
Nucleic Acids - faculty at Chemeketa
... What will be the composition of the DNA strand complementary to –AGCCA– ? a. b. c. d. ...
... What will be the composition of the DNA strand complementary to –AGCCA– ? a. b. c. d. ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".