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... double-stranded DNA and produces a highly fluorescent adduct that can be excited at 488 nm with a broad emission centered around 600 nm. Since PI can also bind to double-stranded RNA, it is necessary to treat the cells with RNase for optimal DNA resolution. ...
... double-stranded DNA and produces a highly fluorescent adduct that can be excited at 488 nm with a broad emission centered around 600 nm. Since PI can also bind to double-stranded RNA, it is necessary to treat the cells with RNase for optimal DNA resolution. ...
Unit 2 Review
... Use the following as a TOOL only. Survey to see what you don’t know, focus on these terms in your notes. This will not be covered in class other than process questions you and several others are concerned about. Questions that are straight from the notes will be re-directed to the notes—find them th ...
... Use the following as a TOOL only. Survey to see what you don’t know, focus on these terms in your notes. This will not be covered in class other than process questions you and several others are concerned about. Questions that are straight from the notes will be re-directed to the notes—find them th ...
AP Bio Molecular Genetics Review Sheet
... the tRNA that recognizes the initiation codon also binds to what? What is the differences in life cycle between virulent phages and temperate phages? The alteration of a cell’s genotype by the uptake of naked, foreign DNA from surrounding environments is called what? What would happen to a cell that ...
... the tRNA that recognizes the initiation codon also binds to what? What is the differences in life cycle between virulent phages and temperate phages? The alteration of a cell’s genotype by the uptake of naked, foreign DNA from surrounding environments is called what? What would happen to a cell that ...
DNA isol
... 3. If you had to choose, be gentle rather that be rough. Doesn’t hurt to be careful when handling material. i.e. keep everything cold, since these enzymes are much more active at physiological temperatures. (i.e. use of "ice cold" this and that") Wear gloves, etc. 4. Know the idiosyncrasies of your ...
... 3. If you had to choose, be gentle rather that be rough. Doesn’t hurt to be careful when handling material. i.e. keep everything cold, since these enzymes are much more active at physiological temperatures. (i.e. use of "ice cold" this and that") Wear gloves, etc. 4. Know the idiosyncrasies of your ...
Mutation and Recombination
... Natural mutation is a very rare event and can only be studied in organisms with very high division rates (such as bacteria). A spontaneous mutation may be detected by using selective media. For example, the antibiotic penicillin, could be added to the culture media. If some of the aseptically transf ...
... Natural mutation is a very rare event and can only be studied in organisms with very high division rates (such as bacteria). A spontaneous mutation may be detected by using selective media. For example, the antibiotic penicillin, could be added to the culture media. If some of the aseptically transf ...
Final review questions: ch 13-15 How does RNA differ from DNA
... reducing the amount of land that is required to grow them. A introducing chemicals into the environment. B increasing an animal's resistance to antibiotics. C changing the genomes of other crop plants. D 24. Genetic markers allow scientists to ...
... reducing the amount of land that is required to grow them. A introducing chemicals into the environment. B increasing an animal's resistance to antibiotics. C changing the genomes of other crop plants. D 24. Genetic markers allow scientists to ...
The Three Domains of Life
... grouped together and called ________________________ - still see this used. Are sometimes referred to as Monera. Advances in __________________ biology identified ________________________________ as unique type of cells (Carl ___________, late 70’s, U of Illinois). They had been thought of as ______ ...
... grouped together and called ________________________ - still see this used. Are sometimes referred to as Monera. Advances in __________________ biology identified ________________________________ as unique type of cells (Carl ___________, late 70’s, U of Illinois). They had been thought of as ______ ...
Gene Expression
... Virus: Viruses are nonliving organisms composed of a protein coat (phage), with the genetic material being stored inside as either DNA or mRNA and reverse transcriptase that creates DNA from mRNA. Viruses require a host cell to survive and reproduce. Lytic Cycle: the lytic cycle occurs when the bact ...
... Virus: Viruses are nonliving organisms composed of a protein coat (phage), with the genetic material being stored inside as either DNA or mRNA and reverse transcriptase that creates DNA from mRNA. Viruses require a host cell to survive and reproduce. Lytic Cycle: the lytic cycle occurs when the bact ...
Analyzing Data
... which allows the DNA to efficiently enter the cells. • The cells are grown in nonselective medium to allow synthesis of plasmid-encoded antibiotic resistance proteins, then plated on antibiotic-containing medium to allow identification of plasmid-containing colonies. ...
... which allows the DNA to efficiently enter the cells. • The cells are grown in nonselective medium to allow synthesis of plasmid-encoded antibiotic resistance proteins, then plated on antibiotic-containing medium to allow identification of plasmid-containing colonies. ...
Name - OnCourse
... 3. The “backbones” of the DNA molecule is made up of two components, what are these? c. _______________________________ d. _______________________________ 5. There are four different bases that make up the “rungs.” What are the names of those bases? a. _______________________________ b. ____________ ...
... 3. The “backbones” of the DNA molecule is made up of two components, what are these? c. _______________________________ d. _______________________________ 5. There are four different bases that make up the “rungs.” What are the names of those bases? a. _______________________________ b. ____________ ...
“Bill Nye: Genes” Video Worksheet
... 9. What can you do with DNA after you take it out of an organism? a. Cut it into smaller pieces b. Place into another organism 10. What 2 organisms were combined to create the message to Bill in the petri dish? Sea Jelly and a bacteria 11. What do genes do? They tell cells what to do 12. Mom tells R ...
... 9. What can you do with DNA after you take it out of an organism? a. Cut it into smaller pieces b. Place into another organism 10. What 2 organisms were combined to create the message to Bill in the petri dish? Sea Jelly and a bacteria 11. What do genes do? They tell cells what to do 12. Mom tells R ...
The Structure of DNA
... Cytosine bonds to Guanine Adenine bonds to Thymine These “bases” make the steps on a ladder The Phosphate and sugar Makes the “Backbone” ...
... Cytosine bonds to Guanine Adenine bonds to Thymine These “bases” make the steps on a ladder The Phosphate and sugar Makes the “Backbone” ...
國立嘉義大學九十一學年度
... 17.Insertion of a DNA molecule (usually by homologous recombination) into a chromosomal site. 18.A bacterial, yeast, or plant cell that has had its cell wall removed either chemically or enzymatically. 19.A DNA sequence (mobile genetic element) that can insert randomly into a chromosome, exit the si ...
... 17.Insertion of a DNA molecule (usually by homologous recombination) into a chromosomal site. 18.A bacterial, yeast, or plant cell that has had its cell wall removed either chemically or enzymatically. 19.A DNA sequence (mobile genetic element) that can insert randomly into a chromosome, exit the si ...
DNA Technology Tools Graphic Organizer KEY
... Makes many copies of an Used for forensic entire region of DNA investigation and in medical testing. Animals that have Used to study diseases received DNA from some and ways to treat them, other organism to improve food supply, disease resistance, and human health. Plants that have received Creates ...
... Makes many copies of an Used for forensic entire region of DNA investigation and in medical testing. Animals that have Used to study diseases received DNA from some and ways to treat them, other organism to improve food supply, disease resistance, and human health. Plants that have received Creates ...
A.D.Hershey and Martha Chase (1952). Independent Function of
... 1902- Waltor Sutton - observed that chromosome segregate in a pattern that match Mendels segregation pattern. 1911- Thomas Hunt Morgan - use Drosophila melanogaster to show chromosomes carry genes. ...
... 1902- Waltor Sutton - observed that chromosome segregate in a pattern that match Mendels segregation pattern. 1911- Thomas Hunt Morgan - use Drosophila melanogaster to show chromosomes carry genes. ...
1. A nucleotide is a ______. 2. DNA consists of two antiparallel
... DNA consists of two antiparallel strands of nucleotide chains held together by The enzyme that catalyzes the synthesis of new DNA molecules is called The function of ribosomes in the cell is _______ Similar to the complementary purine-pyrimidine relationship observed in DNA, which of the following ...
... DNA consists of two antiparallel strands of nucleotide chains held together by The enzyme that catalyzes the synthesis of new DNA molecules is called The function of ribosomes in the cell is _______ Similar to the complementary purine-pyrimidine relationship observed in DNA, which of the following ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".