In vitro conjugal transfer of tetracycline resistance from Lactobacillus
... di¡erent plasmid pro¢le was selected. A total of 13 transconjugants from nine di¡erent donor/recipient combinations were selected for blotting and hybridisation experiments, thereby selecting towards a representation of the maximum in plasmid pro¢le diversity (Fig. 1). In six transconjugants, the pl ...
... di¡erent plasmid pro¢le was selected. A total of 13 transconjugants from nine di¡erent donor/recipient combinations were selected for blotting and hybridisation experiments, thereby selecting towards a representation of the maximum in plasmid pro¢le diversity (Fig. 1). In six transconjugants, the pl ...
The KIebsieIIa pneumoniae cytochrome bd
... and nitrogen-free Davis and Mingioli medium (NFDM medium) (Cannon, 1984), supplemented when required with histidine (at 25 pgml-l) and NH,C1(15 mM). Where indicated, plates were incubated anaerobically in a Gas Pak system (Becton Dickinson). Antibiotics for E. coli and for K . pnuemoniae were added ...
... and nitrogen-free Davis and Mingioli medium (NFDM medium) (Cannon, 1984), supplemented when required with histidine (at 25 pgml-l) and NH,C1(15 mM). Where indicated, plates were incubated anaerobically in a Gas Pak system (Becton Dickinson). Antibiotics for E. coli and for K . pnuemoniae were added ...
Document
... DNA contains the information needed to make proteins. However, DNA is too large to leave the nucleus. RNA acts as a set of working instructions for ribosomes to make proteins. This process is also known as gene expression. Gene expression is a regulated process. ...
... DNA contains the information needed to make proteins. However, DNA is too large to leave the nucleus. RNA acts as a set of working instructions for ribosomes to make proteins. This process is also known as gene expression. Gene expression is a regulated process. ...
Small, Smaller, Smallest: The Origins and Evolution of Ancient Dual
... quadrilineatus (Auchenorrhyncha: Cicadellidae). Our results reveal that Nasuia-ALF has the smallest bacterial genome yet sequenced (112 kb), and that the Sulcia-ALF genome (190 kb) is smaller than that of Sulcia in other insect lineages. Together, these symbionts retain the capability to synthesize ...
... quadrilineatus (Auchenorrhyncha: Cicadellidae). Our results reveal that Nasuia-ALF has the smallest bacterial genome yet sequenced (112 kb), and that the Sulcia-ALF genome (190 kb) is smaller than that of Sulcia in other insect lineages. Together, these symbionts retain the capability to synthesize ...
The sequence of human serum albumin cDNA and its expression in
... An additional cDNA clone extending even farther 5' was obtained by similar oligodeoxynucleotide primed cDNA synthesis (from a primer corresponding to amino acid codons no. 175-179). Although not employed in the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determi ...
... An additional cDNA clone extending even farther 5' was obtained by similar oligodeoxynucleotide primed cDNA synthesis (from a primer corresponding to amino acid codons no. 175-179). Although not employed in the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determi ...
European workshop on Phenotype MicroArrays
... University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada. Experimental technologies such as Phenotype Micro-arrays have provided unprecedented physiological data under different growth environments and genetic backgrounds. Similar advances in computational modeling techniques have allo ...
... University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada. Experimental technologies such as Phenotype Micro-arrays have provided unprecedented physiological data under different growth environments and genetic backgrounds. Similar advances in computational modeling techniques have allo ...
A complete shikimate pathway in Toxoplasma gondii: an ancient
... but no likely candidates were identified for a Class I DAHP synthase. This region was amplified by PCR and used as a probe to screen a T. gondii cDNA library. This produced a number of overlapping clones that assembled to give the entire T. gondii DAHP synthase, which was confirmed by reverse-transc ...
... but no likely candidates were identified for a Class I DAHP synthase. This region was amplified by PCR and used as a probe to screen a T. gondii cDNA library. This produced a number of overlapping clones that assembled to give the entire T. gondii DAHP synthase, which was confirmed by reverse-transc ...
Sample pages 1 PDF
... ammonium oxidizing bacteria (Magalhães et al. 2014). Such studies are normally attempted with the assumption that the primer set would identify the specific gene in all bacteria. But in the absence of a consensus sequence or known conserved sequence for a specific gene this approach could be biased ...
... ammonium oxidizing bacteria (Magalhães et al. 2014). Such studies are normally attempted with the assumption that the primer set would identify the specific gene in all bacteria. But in the absence of a consensus sequence or known conserved sequence for a specific gene this approach could be biased ...
Gene Silencing without DNA: RNA-Mediated Cross
... the RNA-based mechanism induced by PVX is functionally the same as PTGS. In these experiments, nine plants were either mock inoculated or inoculated with PVX–GF, which carries 465 nucleotides of GFP sequence encoding a truncated nonfluorescent protein (Ruiz et al., 1998), as illustrated in Figure 5A ...
... the RNA-based mechanism induced by PVX is functionally the same as PTGS. In these experiments, nine plants were either mock inoculated or inoculated with PVX–GF, which carries 465 nucleotides of GFP sequence encoding a truncated nonfluorescent protein (Ruiz et al., 1998), as illustrated in Figure 5A ...
Characters of Chymosin Gene Isolated from Different Animal A. G. Attallah
... performed with the three sets of primers deduced from chymosin gene sequences [1 1 ,1 2 ] (Table 1) resulted in different bands of approximately 1.2 and 0.2 kb according to the source of the chymosin gene (Fig. 3). The length of pre-prochymosin gene isolated from stomach mucosa of different animal s ...
... performed with the three sets of primers deduced from chymosin gene sequences [1 1 ,1 2 ] (Table 1) resulted in different bands of approximately 1.2 and 0.2 kb according to the source of the chymosin gene (Fig. 3). The length of pre-prochymosin gene isolated from stomach mucosa of different animal s ...
2009 HSC Chemistry Sample Answers
... • Light source – is a hollow cathode lamp, in which some of the element is present and is excited to emit the particular wavelength. • Samples are digested (in acid) to produce aqueous solutions. • Samples absorbance compared with standards of known concentration. (Calibration curve) Applications ...
... • Light source – is a hollow cathode lamp, in which some of the element is present and is excited to emit the particular wavelength. • Samples are digested (in acid) to produce aqueous solutions. • Samples absorbance compared with standards of known concentration. (Calibration curve) Applications ...
Development and application of a positive
... transfection efficiencies and the low number of selectable markers. Presently, it is possible to stably transform four species of Plasmodium, the human parasite Plasmodium falciparum, the primate parasite Plasmodium knowlesi and two rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii ...
... transfection efficiencies and the low number of selectable markers. Presently, it is possible to stably transform four species of Plasmodium, the human parasite Plasmodium falciparum, the primate parasite Plasmodium knowlesi and two rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii ...
Contribution of Horizontal Gene Transfer to Virulence
... surgical site infections, skin and soft tissue infections, pneumonias and the most common: urinary tract infections (Klevens, Edwards et al. 2007). Due to prolonged incubation periods, heterogeneity of populations in facilities and lack of reporting mandates, the exact number of HAIs that occur each ...
... surgical site infections, skin and soft tissue infections, pneumonias and the most common: urinary tract infections (Klevens, Edwards et al. 2007). Due to prolonged incubation periods, heterogeneity of populations in facilities and lack of reporting mandates, the exact number of HAIs that occur each ...
Microbes - KICS Learns
... Microbes are so tiny that they cannot be seen with the naked eye. They can only be seen using a microscope. How many different microbes can you name? ...
... Microbes are so tiny that they cannot be seen with the naked eye. They can only be seen using a microscope. How many different microbes can you name? ...
Cloning and Sequence Analysis of the xylL Gene Responsible for
... restriction endonuclease and ligation of DNA fragments by T4 DNA ligase were performed by standard procedures as recommended by the supplier (Posco Co., Seoul, Korea). The pCS1 and pCSP21 carrying the xylL gene were previously cloned from the chromosomal DNA of Pseudomonas sp. S-47 (9). In this stud ...
... restriction endonuclease and ligation of DNA fragments by T4 DNA ligase were performed by standard procedures as recommended by the supplier (Posco Co., Seoul, Korea). The pCS1 and pCSP21 carrying the xylL gene were previously cloned from the chromosomal DNA of Pseudomonas sp. S-47 (9). In this stud ...
ORF distribution and statistics
... Limitation of "long ORF" - based gene detection • Where to set the threshold (multiple testing!)? • Overlapping ORF • Nucleotide frequency and codon usage • Sequencing errors Error rates of NGS sequencing range from 0.1% to 0.6%, depending on the platform and the ...
... Limitation of "long ORF" - based gene detection • Where to set the threshold (multiple testing!)? • Overlapping ORF • Nucleotide frequency and codon usage • Sequencing errors Error rates of NGS sequencing range from 0.1% to 0.6%, depending on the platform and the ...
A family of diatom-like silicon transporters in the siliceous loricate
... mechanisms underlying this process remains limited, with unrelated silicontransporting proteins found in the eukaryotic clades previously studied. Here, we report the identification of silicon transporter (SIT)-type genes from the siliceous loricate choanoflagellates Stephanoeca diplocostata and Dia ...
... mechanisms underlying this process remains limited, with unrelated silicontransporting proteins found in the eukaryotic clades previously studied. Here, we report the identification of silicon transporter (SIT)-type genes from the siliceous loricate choanoflagellates Stephanoeca diplocostata and Dia ...
04_db_blast
... P-value is the probability of finding a seq. similarity as similar as the observed match if there were really no true matches in the database. E-value ≠ p-value E-value ~ p-value when it is small (say < 0.1) Since we are interested in unusual hits, it is safe to interchange E-value with p-value. E-v ...
... P-value is the probability of finding a seq. similarity as similar as the observed match if there were really no true matches in the database. E-value ≠ p-value E-value ~ p-value when it is small (say < 0.1) Since we are interested in unusual hits, it is safe to interchange E-value with p-value. E-v ...
concepts-of-biology
... in the individual nucleotide sequence of a gene, or differences in the arrangements of genes. Phylogenies based on molecular characters assume that the more similar the sequences are in two organisms, the more closely related they are. Different genes change evolutionarily at different rates and thi ...
... in the individual nucleotide sequence of a gene, or differences in the arrangements of genes. Phylogenies based on molecular characters assume that the more similar the sequences are in two organisms, the more closely related they are. Different genes change evolutionarily at different rates and thi ...
Transcription is the synthesis of RNA under the direction of DNA
... sequence information. Both nucleic acid sequences use complementary language, and the information is simply transcribed, or copied, from one molecule to the other. DNA sequence is enzymatically copied by RNA polymerase to produce a complementary nucleotide RNA strand, called messenger RNA (mRNA), be ...
... sequence information. Both nucleic acid sequences use complementary language, and the information is simply transcribed, or copied, from one molecule to the other. DNA sequence is enzymatically copied by RNA polymerase to produce a complementary nucleotide RNA strand, called messenger RNA (mRNA), be ...
Alus
... majority of Alu insertions occur in non-coding regions and are thought to be evolutionarily neutral. However, an Alu insertion in the NF-1 gene is responsible for neurofibromatosis I, Alu insertions in introns of genes for tissue plasminogen activator (TPA) and angiotensin converter enzyme (ACE) are ...
... majority of Alu insertions occur in non-coding regions and are thought to be evolutionarily neutral. However, an Alu insertion in the NF-1 gene is responsible for neurofibromatosis I, Alu insertions in introns of genes for tissue plasminogen activator (TPA) and angiotensin converter enzyme (ACE) are ...
