SURVEY AND SUMMARY Genomics of bacteria and archaea: the
... amount of sequences from diverse organisms was available prior to these reports, but the first fully sequenced bacterial genome forever changed the state of the art in genome analysis. The availability of complete genomes (i.e. with nearly all the genetic material from the given organism sequenced as ...
... amount of sequences from diverse organisms was available prior to these reports, but the first fully sequenced bacterial genome forever changed the state of the art in genome analysis. The availability of complete genomes (i.e. with nearly all the genetic material from the given organism sequenced as ...
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... resistance gene. Chromosomal DNA was extracted by using Bacterial Genomic DNA isolation kit (Sigma Aldrich, USA) and plasmid of K. pneumoniae was extracted according to Mansi El-Mansi et al. (2000). The integrity of DNA was checked on gel electrophoresis and samples were stored at 20°C. The extracte ...
... resistance gene. Chromosomal DNA was extracted by using Bacterial Genomic DNA isolation kit (Sigma Aldrich, USA) and plasmid of K. pneumoniae was extracted according to Mansi El-Mansi et al. (2000). The integrity of DNA was checked on gel electrophoresis and samples were stored at 20°C. The extracte ...
Identification of prokaryotic homologues indicates an endosymbiotic
... Database searching detected one single clear homologue of mitochondrial AOX among available prokaryotic sequence data, a hypothetical protein encoded within a large contiguously assembled sequence (contig) from the genome sequencing project of the a-proteobacterium N. aromaticivorans (accession no. ...
... Database searching detected one single clear homologue of mitochondrial AOX among available prokaryotic sequence data, a hypothetical protein encoded within a large contiguously assembled sequence (contig) from the genome sequencing project of the a-proteobacterium N. aromaticivorans (accession no. ...
Green Fluorescent Protein: A Reporter Molecule
... chemistry for isolating GFP and using it as a ‘reporter molecule’ in biotechnology. ...
... chemistry for isolating GFP and using it as a ‘reporter molecule’ in biotechnology. ...
The Relationship between Protozoan Populations and Biological
... the size of the total bacterial population than with the nature of the soil organic matter and its rate of decomposition. The importance of protozoa in the soil organic cycle is likely to be relatively greater in soils with a high rate of turnover than in soils with a low rate of turnover for two re ...
... the size of the total bacterial population than with the nature of the soil organic matter and its rate of decomposition. The importance of protozoa in the soil organic cycle is likely to be relatively greater in soils with a high rate of turnover than in soils with a low rate of turnover for two re ...
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... •regulation of lipid, fatty-acid and isoprenoid metabolism (20 ORFs) •lipid and fatty-acid transport (21 ORFs) •other lipid, fatty-acid and isoprenoid metabolism activities (13 ORFs) •metabolism of vitamins, cofactors, and prosthetic groups (86 ORFs) •biosynthesis of vitamins, cofactors, and prosthe ...
... •regulation of lipid, fatty-acid and isoprenoid metabolism (20 ORFs) •lipid and fatty-acid transport (21 ORFs) •other lipid, fatty-acid and isoprenoid metabolism activities (13 ORFs) •metabolism of vitamins, cofactors, and prosthetic groups (86 ORFs) •biosynthesis of vitamins, cofactors, and prosthe ...
Effects of Natural Selection on Interpopulation Divergence
... acting similarly in two populations to reduce frequency of a deleterious allele, the reduction in frequency will be similar in both populations, causing similar allele frequencies in the two populations. As a result, the genetic distance between populations will be reduced at a locus subject to puri ...
... acting similarly in two populations to reduce frequency of a deleterious allele, the reduction in frequency will be similar in both populations, causing similar allele frequencies in the two populations. As a result, the genetic distance between populations will be reduced at a locus subject to puri ...
Microbiological and Immunological Investigation of adult patients
... .(0.3%) . on the other hand fungi were found in (4.2%) and it includes Aspergillus fumigatus(2.5%) and Aspergillus niger (1.7%) . The causative agents of chronic sinusitis include variety of microorganisms and often more than one bacterial species can be found , so it is difficult to compare the res ...
... .(0.3%) . on the other hand fungi were found in (4.2%) and it includes Aspergillus fumigatus(2.5%) and Aspergillus niger (1.7%) . The causative agents of chronic sinusitis include variety of microorganisms and often more than one bacterial species can be found , so it is difficult to compare the res ...
A Transient Expression Assay Using Maize Mesophyll Protoplasts
... home-made machine can provide the condition. Keep electroporated samples on ice for 10 min before resuspension in a 6-well tissue culture dish (Falcon 3046) at 0.5-2 x 105/ml. The dish can be coated with 5% calf serum for 10 sec before use to prevent the sticking of the protoplasts to the plastic su ...
... home-made machine can provide the condition. Keep electroporated samples on ice for 10 min before resuspension in a 6-well tissue culture dish (Falcon 3046) at 0.5-2 x 105/ml. The dish can be coated with 5% calf serum for 10 sec before use to prevent the sticking of the protoplasts to the plastic su ...
Document
... • tblastx compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database. ...
... • tblastx compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database. ...
Multiregional origin of B chromosomes in the grasshopper
... B chromosomes most likely derive from the host genome, albeit intra- or interspecifically. Although the intraspecific hypothesis likely applies to many, perhaps most, B chromosomes, there is sound evidence that some of them have arisen through interspecific hybridization (see McAllister and Werren 1 ...
... B chromosomes most likely derive from the host genome, albeit intra- or interspecifically. Although the intraspecific hypothesis likely applies to many, perhaps most, B chromosomes, there is sound evidence that some of them have arisen through interspecific hybridization (see McAllister and Werren 1 ...
Increase in Tomato Locule Number Is Controlled by Two Single
... whole genome (Van Deynze et al., 2007). A number of genes controlling tomato fruit morphology have been cloned: FW2.2 (Frary et al., 2000) controls fruit weight; SUN (Xiao et al., 2008) and OVATE (Liu et al., 2002) control elongated fruit shape; and FASCIATED (FAS; Cong et al., 2008) controls locule ...
... whole genome (Van Deynze et al., 2007). A number of genes controlling tomato fruit morphology have been cloned: FW2.2 (Frary et al., 2000) controls fruit weight; SUN (Xiao et al., 2008) and OVATE (Liu et al., 2002) control elongated fruit shape; and FASCIATED (FAS; Cong et al., 2008) controls locule ...
Chapter 3 Kinetic analysis of ribozyme cleavage
... migration after renaturation; this could reflect dissociation of a dimer or formation of a more compact tertiary fold (18), both of which are typically desired. Run markers on the gel; double-stranded DNA (dsDNA) markers are readily available from numerous vendors (e.g. New England Biolabs), and doub ...
... migration after renaturation; this could reflect dissociation of a dimer or formation of a more compact tertiary fold (18), both of which are typically desired. Run markers on the gel; double-stranded DNA (dsDNA) markers are readily available from numerous vendors (e.g. New England Biolabs), and doub ...
THE EXPANSION OF CONSERVATION GENETICS
... and microsatellite variation as the main tools of their trade. These researchers are continuously looking for more rapid and efficient screening techniques to detect genetic variability. Finally, some conservation geneticists have even contemplated using microarrays and quantitative-trait mapping in ...
... and microsatellite variation as the main tools of their trade. These researchers are continuously looking for more rapid and efficient screening techniques to detect genetic variability. Finally, some conservation geneticists have even contemplated using microarrays and quantitative-trait mapping in ...
Biodiversity indices for the assessment of air, water and soil quality
... of environmental stress; the lichens considered for the index calculation are, essentially, the epiphytic ones. Specific indications on the sampling system and survey procedures of the lichen biodiversity are available on the Manual for the application of the index, published by ANPA (ANPA, 2001). A ...
... of environmental stress; the lichens considered for the index calculation are, essentially, the epiphytic ones. Specific indications on the sampling system and survey procedures of the lichen biodiversity are available on the Manual for the application of the index, published by ANPA (ANPA, 2001). A ...
Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics
... previously circulating viruses, emerged in the 1957 pandemic. Another virus, A/Hong Kong/1/68 (H3N2), with a different HA subtype from the 1957 pandemic virus, was isolated in 1968. The genes responsible for the emergence of these new surface glycoproteins are thought to have been introduced from av ...
... previously circulating viruses, emerged in the 1957 pandemic. Another virus, A/Hong Kong/1/68 (H3N2), with a different HA subtype from the 1957 pandemic virus, was isolated in 1968. The genes responsible for the emergence of these new surface glycoproteins are thought to have been introduced from av ...
Computational disease gene identification
... that often present with a wide range of phenotypes and generally involve multiple aetiological mechanisms and contributing genes (1,2). In particular, the contribution of each of several genes to the complex disease state is likely to be small, and only the joint effect of several susceptibility gen ...
... that often present with a wide range of phenotypes and generally involve multiple aetiological mechanisms and contributing genes (1,2). In particular, the contribution of each of several genes to the complex disease state is likely to be small, and only the joint effect of several susceptibility gen ...
NUCLEOTIDES AND NUCLEIC ACIDS
... of a functional biological product, whether protein or RNA, is referred to as a gene. A cell typically has many thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broa ...
... of a functional biological product, whether protein or RNA, is referred to as a gene. A cell typically has many thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broa ...
Development of a novel analytical approach combining the quantification of
... essential components such as amino acids. However, the scenario is completely different when analysts wish to tackle the analytical monitoring of a wide number of metabolites, including amino acids, vitamins, and proteins. More recently, despite the complexity of commercial culture media, non-invasi ...
... essential components such as amino acids. However, the scenario is completely different when analysts wish to tackle the analytical monitoring of a wide number of metabolites, including amino acids, vitamins, and proteins. More recently, despite the complexity of commercial culture media, non-invasi ...
BUGS IN THE BEAM - Purdue University Cytometry Laboratories
... droplet is deflected in an electric field into collection vessels for subsequent analysis. The strength of flow cytometry lies in its capacity for single cell measurements, its acquisition speed and its numerical power. The total illumination of the particle in the laser beam allows the quantificati ...
... droplet is deflected in an electric field into collection vessels for subsequent analysis. The strength of flow cytometry lies in its capacity for single cell measurements, its acquisition speed and its numerical power. The total illumination of the particle in the laser beam allows the quantificati ...
Sense and Nonsense in the Genetic Code
... only exceptions known are certain viruses in which messenger RNA is used directly as the genetic material). Most of our present knowledge about the code has been obtained from studies with Escherichiacoli in which synthetic polyribonucleotides (rather than natural messenger RNA) are added to cell ex ...
... only exceptions known are certain viruses in which messenger RNA is used directly as the genetic material). Most of our present knowledge about the code has been obtained from studies with Escherichiacoli in which synthetic polyribonucleotides (rather than natural messenger RNA) are added to cell ex ...
______________________________________________________________________ Análisis de la composición del regulón LexA en el dominio Resumen
... were cloned and sequencied. After sobreexpressing and purifying respective proteins EMSA assays and footprinting were performed, and both regcognition motifs of LexA were defined: GGTT N2 C N4 G N3 ACC for G. sulfurreducens and TGTATC N12 TACA for F. nucleatum. Next, the described motifs were studie ...
... were cloned and sequencied. After sobreexpressing and purifying respective proteins EMSA assays and footprinting were performed, and both regcognition motifs of LexA were defined: GGTT N2 C N4 G N3 ACC for G. sulfurreducens and TGTATC N12 TACA for F. nucleatum. Next, the described motifs were studie ...
S - IBIVU
... for each of those the derivation of a table of similar tripeptides: the number of tripeptides is only a fraction of total number possible. ...
... for each of those the derivation of a table of similar tripeptides: the number of tripeptides is only a fraction of total number possible. ...