high-performance gene expression
... Arabidopsis thaliana using ISOLATE II RNA Plant Kit. The extracted RNA was diluted in a 2-fold serial dilution (15ng, lanes 1-7 respectively) and PCR was performed using MyTaq One-Step RT-PCR Kit. HyperLadder™ 1kb (M). The results illustrate the quality of the RNA obtained, as it can be used for ver ...
... Arabidopsis thaliana using ISOLATE II RNA Plant Kit. The extracted RNA was diluted in a 2-fold serial dilution (15ng, lanes 1-7 respectively) and PCR was performed using MyTaq One-Step RT-PCR Kit. HyperLadder™ 1kb (M). The results illustrate the quality of the RNA obtained, as it can be used for ver ...
Trophic roles of heterotrophic nanoflagellates and ciliates among
... During the study period, 5 dominant genera of ciliates were found (Table 1). Judging from the microscopic observation of FLB ingestion by ciliates, we confirmed that Cyclidium spp. and Halteria spp. were consumers of bacteria. Although Cinetochilum sp. is classified as a bacterial consumer in the li ...
... During the study period, 5 dominant genera of ciliates were found (Table 1). Judging from the microscopic observation of FLB ingestion by ciliates, we confirmed that Cyclidium spp. and Halteria spp. were consumers of bacteria. Although Cinetochilum sp. is classified as a bacterial consumer in the li ...
The genomic sequence of Exiguobacterium chiriqhucha str
... pairwise comparisons for the three closest strains using the web server from Kostas lab http://enve-omics.ce.gatech.edu/ with default parameters. Comparisons to Exiguobacterium str. N139 can be seen in Table S2. To explore the genomic rearrangements present on E. chiriqhucha str. N139 in comparison ...
... pairwise comparisons for the three closest strains using the web server from Kostas lab http://enve-omics.ce.gatech.edu/ with default parameters. Comparisons to Exiguobacterium str. N139 can be seen in Table S2. To explore the genomic rearrangements present on E. chiriqhucha str. N139 in comparison ...
Genetics revisited - Institut Montefiore
... These were originally discovered in 1868 by Friedrich Meischer (isolating DNA from pus cells on bandages). At that time, he could not confirm that nucleic acids might contain genetic information. DNA IS the genetic information of most living organisms. In contrast, some viruses (called retroviruse ...
... These were originally discovered in 1868 by Friedrich Meischer (isolating DNA from pus cells on bandages). At that time, he could not confirm that nucleic acids might contain genetic information. DNA IS the genetic information of most living organisms. In contrast, some viruses (called retroviruse ...
K-12 MG1655 Escherichia coli Blocks the Aerobic
... cyoABCD, and cbdAB) and a quinol monooxygenase gene (ygiN) from the E. coli K-12 MG1655 genome resulted in the activation of ArcA aerobically. These mutations resulted in reduction of the oxygen uptake rate by nearly 98% and production of D-lactate as a sole by-product under oxic and anoxic conditio ...
... cyoABCD, and cbdAB) and a quinol monooxygenase gene (ygiN) from the E. coli K-12 MG1655 genome resulted in the activation of ArcA aerobically. These mutations resulted in reduction of the oxygen uptake rate by nearly 98% and production of D-lactate as a sole by-product under oxic and anoxic conditio ...
DISSOLUTION METHOD DEVELOPMENT OF LAMIVUDINE, NEVIRAPINE AND STAVUDINE IN TABLETS BY UPLC
... performing on UPLC because of its less time consumption and high sensitivity. The other factors to consider for developing UPLC methods are reduction of total run time, lower cost per assay, dissolution and promote instrument uptime. The advantage of UPLC is the chromatogram ge ...
... performing on UPLC because of its less time consumption and high sensitivity. The other factors to consider for developing UPLC methods are reduction of total run time, lower cost per assay, dissolution and promote instrument uptime. The advantage of UPLC is the chromatogram ge ...
Proteus mirabilis Tigecycline (GAR-936) in with Reduced Levels of
... other. The acrA gene of P. mirabilis also had 75% amino acid identity to the acrA genes found in E. coli, K. pneumoniae, and E. aerogenes. The plasmid pCLL3430, containing a 5,128-bp acrRAB gene fragment from P. mirabilis G151, was altered by inserting a gentamicin resistance cassette into the multi ...
... other. The acrA gene of P. mirabilis also had 75% amino acid identity to the acrA genes found in E. coli, K. pneumoniae, and E. aerogenes. The plasmid pCLL3430, containing a 5,128-bp acrRAB gene fragment from P. mirabilis G151, was altered by inserting a gentamicin resistance cassette into the multi ...
ESCMID Online Lecture Library © by author
... • The transposase recognizes and binds to the transposable element • It then acts as a site-specific endonuclease, cleaving the DNA to expose the element's 3'-OH ends • A duplex target DNA then binds to the complex • Direct attack of the 3'-OH ends on phosphates of the duplex cleaving it and joining ...
... • The transposase recognizes and binds to the transposable element • It then acts as a site-specific endonuclease, cleaving the DNA to expose the element's 3'-OH ends • A duplex target DNA then binds to the complex • Direct attack of the 3'-OH ends on phosphates of the duplex cleaving it and joining ...
Compilation of tRNA sequences and sequences of
... added. This compilation is updated up to December 2002. The table contains the known tRNA sequences of all organisms including organelles. This is the continuation of the original tRNA compilation first published in 1978. (iii) Compilation of tRNA Genes, is a summary of the sequences of tRNA genes p ...
... added. This compilation is updated up to December 2002. The table contains the known tRNA sequences of all organisms including organelles. This is the continuation of the original tRNA compilation first published in 1978. (iii) Compilation of tRNA Genes, is a summary of the sequences of tRNA genes p ...
a-Aminoadipate aminotransferase from an extremely
... degenerate primers for PCR to clone a DNA fragment containing a Kat2 homologue of T. thermophilus HB27. An amplified 666 bp fragment was blunted, phosphorylated and ligated into the EcoRV site of pBluescript II KS (+) (Strategene). The resulting plasmid was named pKSAAA101. Based on the information ...
... degenerate primers for PCR to clone a DNA fragment containing a Kat2 homologue of T. thermophilus HB27. An amplified 666 bp fragment was blunted, phosphorylated and ligated into the EcoRV site of pBluescript II KS (+) (Strategene). The resulting plasmid was named pKSAAA101. Based on the information ...
results and discussion discussion
... sample. The water appeared greenish-brown in colour and had a pH of 8.5. Microscopic observation revealed presence of bacterial, cyanobacterial, filamentous algal cells, and few possible members of invertebrate groups. The pond water metagenomic DNA was isolated by the in situ lysis method as descri ...
... sample. The water appeared greenish-brown in colour and had a pH of 8.5. Microscopic observation revealed presence of bacterial, cyanobacterial, filamentous algal cells, and few possible members of invertebrate groups. The pond water metagenomic DNA was isolated by the in situ lysis method as descri ...
The Phylogeny of the Cercomeria (Platyhelminthes: Rhabdocoela
... phylogenetic congruence between anatomical and ultrastructural data gathered by different research groups. Brooks et al. (1985b) presented a familial-level phylogenetic systematic analysis of the Digenea, based on 158 characters with a consistency index of 73.5% (215 transformations for the 158 char ...
... phylogenetic congruence between anatomical and ultrastructural data gathered by different research groups. Brooks et al. (1985b) presented a familial-level phylogenetic systematic analysis of the Digenea, based on 158 characters with a consistency index of 73.5% (215 transformations for the 158 char ...
Emmission Spectroscopy
... Luminescence: Process, in which susceptible molecules emit light from electronically excited states created by either a physical (for example, absorption of light), mechanical (friction), or chemical mechanism. Photoluminescence: Generation of luminescence through excitation of a molecule by ultravi ...
... Luminescence: Process, in which susceptible molecules emit light from electronically excited states created by either a physical (for example, absorption of light), mechanical (friction), or chemical mechanism. Photoluminescence: Generation of luminescence through excitation of a molecule by ultravi ...
RNAzol RT (R4533) - Technical Bulletin - Sigma
... DNA, RNA, and protein on homogenization or lysis of tissue sample. The addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can be removed by centrifugation. RNA can then be isolated from the supernatant by alcohol precipitation, ...
... DNA, RNA, and protein on homogenization or lysis of tissue sample. The addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can be removed by centrifugation. RNA can then be isolated from the supernatant by alcohol precipitation, ...
Molecular Evolution of Functional Nucleic Acids
... Nucleic acid aptamers have attracted keen interest, especially for their potential medical uses. For applications in the medical field, improvement of nuclease resistance, i.e., biostability in serum or cells as well as affinity to target molecules, became an important issue. An effective solution i ...
... Nucleic acid aptamers have attracted keen interest, especially for their potential medical uses. For applications in the medical field, improvement of nuclease resistance, i.e., biostability in serum or cells as well as affinity to target molecules, became an important issue. An effective solution i ...
Defensive microbial symbionts in Hymenoptera Martin Kaltenpoth
... the gut represents one of the most sensitive entry routes for pathogens (Siva-Jothy et al. 2005). The first empirical support for this hypothesis came from studies on the locust Schistocerca gregaria (Dillon & Charnley 1995). Locust feces contain at least three different quinines, which significantl ...
... the gut represents one of the most sensitive entry routes for pathogens (Siva-Jothy et al. 2005). The first empirical support for this hypothesis came from studies on the locust Schistocerca gregaria (Dillon & Charnley 1995). Locust feces contain at least three different quinines, which significantl ...
Guanine-Plus-Cytosine Content of Rothia dentocaviosa
... than those published by Slack and Gerenscer (10) (51 mol% G+C for strain ATCC 17931T)and are far below the values in an earlier publication (4) (69.7 mol% G+C for the filamentous form of strain ATCC 17931T).The value for the clinical isolate was 52.9 mol% (range for four determinations, 52.0 to 53.3 ...
... than those published by Slack and Gerenscer (10) (51 mol% G+C for strain ATCC 17931T)and are far below the values in an earlier publication (4) (69.7 mol% G+C for the filamentous form of strain ATCC 17931T).The value for the clinical isolate was 52.9 mol% (range for four determinations, 52.0 to 53.3 ...
Bio2 Gene prediction DNA structure Codons and ORFs Predicting
... • Are there features of this protein similar to others? ...
... • Are there features of this protein similar to others? ...
Structure analysis of DNA relaxases, the key enzymes of bacterial
... protein constructs was monitored by measuring the change in the circular dichroism (CD) signal at 220 nm upon temperature change. The unfolding transition of both proteins occurred at around 42°C. CD spectra measured at 20°C showed 30 % α-helix and 13 % β-sheet for TraA and 27 % α-helix and 18 % β-s ...
... protein constructs was monitored by measuring the change in the circular dichroism (CD) signal at 220 nm upon temperature change. The unfolding transition of both proteins occurred at around 42°C. CD spectra measured at 20°C showed 30 % α-helix and 13 % β-sheet for TraA and 27 % α-helix and 18 % β-s ...
Environmental bacteriophages: viruses of microbes in aquatic
... that consist only of cellular organisms (Woese et al., 1990). Because viruses lack the ribosomal RNA nucleotide sequence upon which these cellular domains of life are based, they cannot be integrated into the cellular tree of life (Breitbart et al., 2002; Rohwer and Edwards, 2002), although suscepti ...
... that consist only of cellular organisms (Woese et al., 1990). Because viruses lack the ribosomal RNA nucleotide sequence upon which these cellular domains of life are based, they cannot be integrated into the cellular tree of life (Breitbart et al., 2002; Rohwer and Edwards, 2002), although suscepti ...
Identification and temporal expression of putative circadian clock
... butterfly (Reppert, 2006) are comparable to those of T. saltator. This brings the relevance of comparative clock biology sharply into focus; detailed analysis of a diverse non-model species may reveal commonalities or differences that give insight into how each has evolved and functions. Thus, T. sa ...
... butterfly (Reppert, 2006) are comparable to those of T. saltator. This brings the relevance of comparative clock biology sharply into focus; detailed analysis of a diverse non-model species may reveal commonalities or differences that give insight into how each has evolved and functions. Thus, T. sa ...
Fractionation of rice glutelin polypeptides using gel filtration
... would likely confound attempts to further characterize or fractionate the individual polypeptides. Gel filtration chromatography, using Sepharose CL-6B, was effective for partially purifying crude glutelin extracts and obtaining preparative samplesof dissociatedglutelin polypeptides suitable for fur ...
... would likely confound attempts to further characterize or fractionate the individual polypeptides. Gel filtration chromatography, using Sepharose CL-6B, was effective for partially purifying crude glutelin extracts and obtaining preparative samplesof dissociatedglutelin polypeptides suitable for fur ...
Microbial Cause of Calcium Lactate Defect in Cheddar Cheese
... However, a direct enzyme activity wasn’t done to confirm this. Observation: Preliminary trial bacterial counts and lactic acid content compared to experimental trials. ...
... However, a direct enzyme activity wasn’t done to confirm this. Observation: Preliminary trial bacterial counts and lactic acid content compared to experimental trials. ...