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MICROBES IN HUMAN WELFARE.pmd
MICROBES IN HUMAN WELFARE.pmd

... Biogas is a mixture of gases (containing predominantly methane) produced by the microbial activity and which may be used as fuel. You have learnt that microbes produce different types of gaseous end-products during growth and metabolism. The type of the gas produced depends upon the microbes and the ...
Pan-cancer analysis of the metabolic reaction network
Pan-cancer analysis of the metabolic reaction network

... Figure 2 – Classification of reactions based on their presence across 917 cancer genome-scale metabolic models. (A) Depiction of reaction classification. Core reactions are present in virtually all models, either by means of the same gene-reaction association (pan) or via different isoenzymes (iso). ...
basic laboratory course 3
basic laboratory course 3

... charge will bind to a positively charged matrix. Thus, such a matrix can be used to separate negatively charged acidic proteins (which will interact with the matrix and be retained) from positively charged proteins (which will flow through the matrix). The acidic proteins can then be removed or elut ...
Genetics revisited - Institut Montefiore
Genetics revisited - Institut Montefiore

... These were originally discovered in 1868 by Friedrich Meischer (isolating DNA from pus cells on bandages). At that time, he could not confirm that nucleic acids might contain genetic information.  DNA IS the genetic information of most living organisms. In contrast, some viruses (called retroviruse ...
Document
Document

... behaviour of the profile on its extremities which can force to match the whole domain or produce partial matches. ...
2‐1 GENOMIC ANALYSIS REVEALS MULTIPLE [FeFe
2‐1 GENOMIC ANALYSIS REVEALS MULTIPLE [FeFe

Cell-Free Phospholipid Biosynthesis by Gene
Cell-Free Phospholipid Biosynthesis by Gene

Journal of Biotechnology
Journal of Biotechnology

... peptidyl- as well as in acyl- and aryl-carrier-proteins/domains (PCP, ACP, ArCP), – to generate their active holo-forms. This occurs by tethering the phosphopantetheinyl moiety of the cosubstrate coenzyme A (CoA) in phosphodiester linkage to the hydroxymethyl side chain of the conserved active serin ...
Polish Journal of Microbiology - Polskie Towarzystwo Mikrobiologów
Polish Journal of Microbiology - Polskie Towarzystwo Mikrobiologów

Comprehensive Analysis of Hyrdrogen Bonds in Regulatory Protein
Comprehensive Analysis of Hyrdrogen Bonds in Regulatory Protein

... Matthews (1988), who examined three DNA-protein complexes known at that time and concluded that there is ‘‘no code for recognition’’. In a recent review Pabo & Sauer (1992) summarized the pairs of amino acid-base in eight complexes and pointed out frequent interactions, such as hydrogen bonds involv ...
MIAPE_Quant_v1.0_Template
MIAPE_Quant_v1.0_Template

... (mapped back from each peptide), together with their estimated confidence. Report each quantification value together with the appropriate information to identify the feature or peptide that is quantified (peptide sequence or peptide sequence plus the charge state, mass, retention time, etc…). 5.1.1 ...
Database searching
Database searching

Ammonia (plasma, blood) - Association for Clinical Biochemistry
Ammonia (plasma, blood) - Association for Clinical Biochemistry

... Note that due to the high risk of an elevated [ammonia] caused by  mishandling of the sample or delay in its reaching the laboratory, it is  advisable that the analysis should be repeated on a subsequent sample to  confirm the result. This is especially true if the initial measurement was  performed ...
Deriving phylogenetic trees from the similarity analysis of metabolic
Deriving phylogenetic trees from the similarity analysis of metabolic

... series of individual chemical reactions in a living system that combine to perform one or more important functions. The product of one reaction in a pathway serves as the substrate for the following reaction. Recently a few methods that use organisms' metabolic pathways to compute a phylogenetic tre ...
Couchioplanes caeruleus - International Journal of Systematic and
Couchioplanes caeruleus - International Journal of Systematic and

... DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate sequence templates, a PCR was performed twice. First, 16s rRNA genes were amplified by a PCR by using a standar ...
Dot Plot - APBioNET
Dot Plot - APBioNET

... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
Preliminary Examination of the Bacterial Diversity
Preliminary Examination of the Bacterial Diversity

... were scored for morphological characteristics and subjected to a variety of biochemical tests. The results of these analyses revealed 65 unique isolates, four of which could not be sequenced. The 16s rRNA gene was sequenced for each of these isolates in order to classify each to genus and species. B ...
Monitoring Changes in Genetic Diversity (PDF Available)
Monitoring Changes in Genetic Diversity (PDF Available)

... these data available according to global data standards. Until now biodiversity indicators have largely overlooked data from the molecular tools that are available for measuring variation at the DNA level, partly due to their limited availability, high expense, and inaccessibility, focusing instead ...
ppt - University of Connecticut
ppt - University of Connecticut



Classification and Phylogenetic Analysis of the cAMP
Classification and Phylogenetic Analysis of the cAMP

Biochemistry – Problem Set 2 Problem Set 2
Biochemistry – Problem Set 2 Problem Set 2

... de-protonated. Only at pH~3 would a fraction of either of these amino acids be found with their a-COOH (pKa~2) de-protonated and the side-chain COOH (pKa~4) protonated. (2 pts) 2. (10 pts) The structure of Asp-Gly-Arg is drawn below. (5 pts for structure, 2 pts for correct assignment of amino and ca ...
HYBRIDIZATION BETWEEN AN INVASIVE AND A NATIVE
HYBRIDIZATION BETWEEN AN INVASIVE AND A NATIVE

Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase

... were amplified by PCR, cloned, and sequenced. Sequence analysis revealed that there are two types of ZmIPK1 genomic sequences. One genomic fragment is identical to the ZmIPK1A genomic sequence obtained from isolated BAC clones (Fig. 2), and the other, designated as ZmIPK1B (GenBank accession: EF5278 ...
Immobilization_Mecha..
Immobilization_Mecha..

... Accepted: September 3, 2012 Published: September 4, 2012 ...
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