Molecular mechanisms of bacterial resistance to antimicrobial agents
... [27]. The other less studied mechanism of enzymatic degradation is the hydrolysis of the carbon-phosphorus bond in the epoxide antibiotic fosfomycin. This may be enzymatically achieved by a C-P lyase enzyme complex in many Gramnegative soil bacteria [28]. The second mechanism of antibiotic inactivat ...
... [27]. The other less studied mechanism of enzymatic degradation is the hydrolysis of the carbon-phosphorus bond in the epoxide antibiotic fosfomycin. This may be enzymatically achieved by a C-P lyase enzyme complex in many Gramnegative soil bacteria [28]. The second mechanism of antibiotic inactivat ...
DEPARTMENT OF BIOCHEMISTRY UNIVERSITY OF KERALA
... functions of the cells are uniquely maintained by four major biomolecules namely carbohydrates, lipids, proteins and nucleic acids. The course deals in detail with study of definition, classification, structure and cellular functions of biomolecules- carbohydrates, lipids, proteins and nucleic acids ...
... functions of the cells are uniquely maintained by four major biomolecules namely carbohydrates, lipids, proteins and nucleic acids. The course deals in detail with study of definition, classification, structure and cellular functions of biomolecules- carbohydrates, lipids, proteins and nucleic acids ...
Attachment 2 - Food Standards Australia New Zealand
... sequence. Polymerase chain reaction (PCR) analyses were performed to verify the sequences at the 5' and 3' ends of the insert. Insert and copy number Southern hybridisation was used to determine the number and nature of DNA insertions in line H7-1. The genomic DNA was digested and probed with an int ...
... sequence. Polymerase chain reaction (PCR) analyses were performed to verify the sequences at the 5' and 3' ends of the insert. Insert and copy number Southern hybridisation was used to determine the number and nature of DNA insertions in line H7-1. The genomic DNA was digested and probed with an int ...
Changes in composition and abundance of functional groups of
... The arctic tundra is considered a maritime biome, as approximately 80% of nonalpine tundra is located within 100 km of a coastline [1]. As a result of the retreating sea ice, arctic land surface temperatures are increasing, causing major changes in terrestrial ecosystems [2,3]. In response to warmin ...
... The arctic tundra is considered a maritime biome, as approximately 80% of nonalpine tundra is located within 100 km of a coastline [1]. As a result of the retreating sea ice, arctic land surface temperatures are increasing, causing major changes in terrestrial ecosystems [2,3]. In response to warmin ...
PDF
... Kary Mullis and others contributors found that this enzyme could be used in the polymerase chain reaction (PCR) to amplify DNA fragments (Chien et al., 1976; Saiki et al., 1988). The development of that technique, as well as the enhancement of molecular cloning techniques using plasmids as vectors, ...
... Kary Mullis and others contributors found that this enzyme could be used in the polymerase chain reaction (PCR) to amplify DNA fragments (Chien et al., 1976; Saiki et al., 1988). The development of that technique, as well as the enhancement of molecular cloning techniques using plasmids as vectors, ...
Recent Developments in Solid-Phase Extraction for Near and
... excited to perform stretching-, deformation- and scissor-vibrations. In contrast to mid-infrared (MIR, 4000–400 cm´1 ), where only fundamental vibrations (“signatures”) can be observed, overtones and combination vibrations are obtained in the NIR region resulting in additional information compared t ...
... excited to perform stretching-, deformation- and scissor-vibrations. In contrast to mid-infrared (MIR, 4000–400 cm´1 ), where only fundamental vibrations (“signatures”) can be observed, overtones and combination vibrations are obtained in the NIR region resulting in additional information compared t ...
The endogenous molecular clock orchestrates the temporal
... Blood and Tissue Kit (Qiagen, Venlo, Netherlands). To assess recombination specificity, PCR was performed with tissue DNA and primers for the recombined and non-recombined alleles as described in Storch et al. [50]. The forward and reverse primers for the nonrecombined allele were the same as the ge ...
... Blood and Tissue Kit (Qiagen, Venlo, Netherlands). To assess recombination specificity, PCR was performed with tissue DNA and primers for the recombined and non-recombined alleles as described in Storch et al. [50]. The forward and reverse primers for the nonrecombined allele were the same as the ge ...
Molecular Characterization of Visual Pigments in
... eyes and ocelli. Clone development was achieved by PCR using 0.5 U of Platinum Taq DNA polymerase (Invitrogen) at a final concentration of 1 PCR Buffer minus Mg (Invitrogen), 0.2 mM of deoxynucleoside triphosphate (dNTP) mixture which contains all 4 deoxynucleotides (dATP, dCTP, dGTP, and dTTP), an ...
... eyes and ocelli. Clone development was achieved by PCR using 0.5 U of Platinum Taq DNA polymerase (Invitrogen) at a final concentration of 1 PCR Buffer minus Mg (Invitrogen), 0.2 mM of deoxynucleoside triphosphate (dNTP) mixture which contains all 4 deoxynucleotides (dATP, dCTP, dGTP, and dTTP), an ...
Regulation of nitrogen metabolism in gram
... by GlnR in various bacteria. Therefore, the regulatory sites of the glnRA operon from all bacteria were included in a training set for constructing the signal. Application of a signal recognition procedure allowed us to identify a common regulatory site with consensus ATGTNAWWWWWWWTNACAT, which diff ...
... by GlnR in various bacteria. Therefore, the regulatory sites of the glnRA operon from all bacteria were included in a training set for constructing the signal. Application of a signal recognition procedure allowed us to identify a common regulatory site with consensus ATGTNAWWWWWWWTNACAT, which diff ...
ARF-Aux/IAA interactions through domain III/IV are not strictly
... than the nph4−1/arf7 protoplast assays in response to auxin and suggest that employing integrated reporter genes in protoplast did not require an ARF activator effector gene to observe an auxin transfection assays11,15 may provide additional insight into how response. For these reasons, the nph4−1/a ...
... than the nph4−1/arf7 protoplast assays in response to auxin and suggest that employing integrated reporter genes in protoplast did not require an ARF activator effector gene to observe an auxin transfection assays11,15 may provide additional insight into how response. For these reasons, the nph4−1/a ...
Protein Signatures Distinctive of Alpha Proteobacteria and Its
... known that can distinguish these bacteria from other groups. The evolutionary relationships among this group, which includes numerous pathogens and agriculturally important microbes, are also not understood. Shared conserved inserts and deletions (i.e., indels or signatures) in molecular sequences p ...
... known that can distinguish these bacteria from other groups. The evolutionary relationships among this group, which includes numerous pathogens and agriculturally important microbes, are also not understood. Shared conserved inserts and deletions (i.e., indels or signatures) in molecular sequences p ...
Developmental Regulation of Genes Encoding Universal Stress
... Gene Regulation and Systems Biology 2011:5 61–74 doi: 10.4137/GRSB.S7491 This article is available from http://www.la-press.com. © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is ...
... Gene Regulation and Systems Biology 2011:5 61–74 doi: 10.4137/GRSB.S7491 This article is available from http://www.la-press.com. © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is ...
The merging of community ecology and phylogenetic biology
... traits may be labile towards the tips of the phylogeny (less inclusive phylogenetic scale) because close relatives often have divergent or labile traits as a result of character displacement and ⁄ or adaptive radiation or due to drift and ⁄ or divergent selection following allopatric speciation. At ...
... traits may be labile towards the tips of the phylogeny (less inclusive phylogenetic scale) because close relatives often have divergent or labile traits as a result of character displacement and ⁄ or adaptive radiation or due to drift and ⁄ or divergent selection following allopatric speciation. At ...
Full Text - Malaysian Journal of Microbiology
... monomers via a bacterial strain depends on the carbon substrate and the substrate specificity of the PHA synthase. The incorporation of various kinds and ratio of monomers determines the properties of the polymer. Poly(3-hydroxybutyrate), P(3HB) is the most common type of PHA. However, the applicati ...
... monomers via a bacterial strain depends on the carbon substrate and the substrate specificity of the PHA synthase. The incorporation of various kinds and ratio of monomers determines the properties of the polymer. Poly(3-hydroxybutyrate), P(3HB) is the most common type of PHA. However, the applicati ...
Techniques in basic microbiology
... illuminated by light passing through an annular (ring shaped) diaphragm. Direct light rays (unaltered by the specimen) travel a different path than light rays that are reflected or diffracted as they pass through the specimen. These two sets of rays are combined at the eye. Reflected or diffracted l ...
... illuminated by light passing through an annular (ring shaped) diaphragm. Direct light rays (unaltered by the specimen) travel a different path than light rays that are reflected or diffracted as they pass through the specimen. These two sets of rays are combined at the eye. Reflected or diffracted l ...
Inquiry into Life Twelfth Edition
... • The lac operon was the first operon discovered • It contains 3 genes coding for E. coli proteins that permit the bacteria to use the sugar lactose – Galactoside permease (lacY) which transports lactose into the cells b-galactosidase (lacZ) cuts the lactose into galactose and glucose – Galactosid ...
... • The lac operon was the first operon discovered • It contains 3 genes coding for E. coli proteins that permit the bacteria to use the sugar lactose – Galactoside permease (lacY) which transports lactose into the cells b-galactosidase (lacZ) cuts the lactose into galactose and glucose – Galactosid ...
The merging of community ecology and phylogenetic
... traits may be labile towards the tips of the phylogeny (less inclusive phylogenetic scale) because close relatives often have divergent or labile traits as a result of character displacement and ⁄ or adaptive radiation or due to drift and ⁄ or divergent selection following allopatric speciation. At ...
... traits may be labile towards the tips of the phylogeny (less inclusive phylogenetic scale) because close relatives often have divergent or labile traits as a result of character displacement and ⁄ or adaptive radiation or due to drift and ⁄ or divergent selection following allopatric speciation. At ...
HUMAN PRIMARY CELLS RNA PRODUCTS Total RNA
... allows us to observe the 18s and 28s ribosomal RNA bands, which are another indicator of good RNA quality. Q. Why does my RNA product come in DEPC-treated water? A. AllCells stores and ships RNA products in DEPC-treated water which is nuclease-free, and can maintain quality and integrity of the RNA ...
... allows us to observe the 18s and 28s ribosomal RNA bands, which are another indicator of good RNA quality. Q. Why does my RNA product come in DEPC-treated water? A. AllCells stores and ships RNA products in DEPC-treated water which is nuclease-free, and can maintain quality and integrity of the RNA ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... multicellular communities of microorganisms attached to a surface or interface. Bacteria seem to initiate biofilm formation in response to specific environmental cues, such as nutrient and oxygen availability. Biofilm are the source of persistent infections of many pathogenic microbes. They are resp ...
... multicellular communities of microorganisms attached to a surface or interface. Bacteria seem to initiate biofilm formation in response to specific environmental cues, such as nutrient and oxygen availability. Biofilm are the source of persistent infections of many pathogenic microbes. They are resp ...
Phylogenies and pheromones
... thereby representing a good indicator for the male’s fitness, because only a superior, healthy male is able to develop and maintain such costly signals (Ahtiainen et al. 2005; McGraw et al. 2002; Rantala et al. 2002; Rantala & Kortet 2004; Zahavi 1975). When females choose a male of high quality as ...
... thereby representing a good indicator for the male’s fitness, because only a superior, healthy male is able to develop and maintain such costly signals (Ahtiainen et al. 2005; McGraw et al. 2002; Rantala et al. 2002; Rantala & Kortet 2004; Zahavi 1975). When females choose a male of high quality as ...
BIODIVERSITY AND CONSERVATION
... • THE VARIETY OF LIVING ORGANISMS • RECOGNISED BY LEOPOLD IN 1933 • NUMBER OF SPP OR SPP ABUNDANCE IS ALPHA (α) SPP DIVERSITY • BETA (β) SPP DIVERSITY COMPARES ONE ECOSYSTEM WITH ANOTHER ECOSYSTEM • GAMMA (γ ) SPP DIVERSITY IS THE # OF SPP OVER A BROAD GEOGRAPHIC AREA WITH NO REFERENCE TO ABUNDANCE ...
... • THE VARIETY OF LIVING ORGANISMS • RECOGNISED BY LEOPOLD IN 1933 • NUMBER OF SPP OR SPP ABUNDANCE IS ALPHA (α) SPP DIVERSITY • BETA (β) SPP DIVERSITY COMPARES ONE ECOSYSTEM WITH ANOTHER ECOSYSTEM • GAMMA (γ ) SPP DIVERSITY IS THE # OF SPP OVER A BROAD GEOGRAPHIC AREA WITH NO REFERENCE TO ABUNDANCE ...
Zebra Finch Glucokinase Containing Two Homologous Halves Is an
... So, in our opinion, Zebra finch possesses normal functional glucokinase gene and normal functional hexokinase II gene which are situated on the same chromosome 22 near each other. Interestingly, in Figure 3 both N- and C-halves of Turkey hexokinase I can be found on branches with Nand C-halves of HK ...
... So, in our opinion, Zebra finch possesses normal functional glucokinase gene and normal functional hexokinase II gene which are situated on the same chromosome 22 near each other. Interestingly, in Figure 3 both N- and C-halves of Turkey hexokinase I can be found on branches with Nand C-halves of HK ...
Performance Characteristics of Three Assays for the Therapeutic
... MTX is an antifolate drug commonly used as an antineoplastic agent. High-dose MTX is used in the treatment of certain malignancies including leptomeningeal metastases, systemic non-Hodgkin lymphoma, acute lymphoblastic leukemia and osteosarcoma. Low-dose MTX is commonly used in the treatment of vari ...
... MTX is an antifolate drug commonly used as an antineoplastic agent. High-dose MTX is used in the treatment of certain malignancies including leptomeningeal metastases, systemic non-Hodgkin lymphoma, acute lymphoblastic leukemia and osteosarcoma. Low-dose MTX is commonly used in the treatment of vari ...
Class :- XII
... Name and explain the type of barrier of innate immunity, where some cells release interferon when infected. Why is Human Genome Project called a mega project? A person has been diagnosed to be HIV positive. (i) Name the test which the person has undergone. (ii) Write the full name of the pathogen in ...
... Name and explain the type of barrier of innate immunity, where some cells release interferon when infected. Why is Human Genome Project called a mega project? A person has been diagnosed to be HIV positive. (i) Name the test which the person has undergone. (ii) Write the full name of the pathogen in ...