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Bacteriophage functional genomics and its role in
... genes, which are expressed upon infection and transcription of phage genes [20–23] and the biocontrol of pathogenic bacteria in food [24–27] (reviewed in [28]). Bacteriophages, especially those featuring a temperate lifestyle, are also the major driving force of horizontal gene exchange and bacteria ...
... genes, which are expressed upon infection and transcription of phage genes [20–23] and the biocontrol of pathogenic bacteria in food [24–27] (reviewed in [28]). Bacteriophages, especially those featuring a temperate lifestyle, are also the major driving force of horizontal gene exchange and bacteria ...
Paper metal ions-RESUB2_logged
... employed to study the influence of metal ions on the whole organism phenotype of P. putida DOT-T1E strains in the presence and absence of toluene. To ensure that there was sufficient biomass for metabolomics analysis, cultures were grown in LB medium supplemented without or with 7 mM magnesium and 3 ...
... employed to study the influence of metal ions on the whole organism phenotype of P. putida DOT-T1E strains in the presence and absence of toluene. To ensure that there was sufficient biomass for metabolomics analysis, cultures were grown in LB medium supplemented without or with 7 mM magnesium and 3 ...
DEVELOPMENT OF FLUORESCENT IN SITU HYBRIDIZATION FOR
... before detection (Bouvier & Giorgio, 2003). The techniques described in this study can be completed within a working day, which is rapid compared to classic microbial detection methods that usually require culturing before biochemical or molecular identification can be completed (Buchbinder, Trebesi ...
... before detection (Bouvier & Giorgio, 2003). The techniques described in this study can be completed within a working day, which is rapid compared to classic microbial detection methods that usually require culturing before biochemical or molecular identification can be completed (Buchbinder, Trebesi ...
GUIDE (download pdf)
... medium in which the target microorganisms grow and are detected by optically sensitive reagents. The reagents change their spectral patterns as the metabolic process takes place. These changes are detected photometrically by an optical instrument and monitored at predetermined time intervals. The ke ...
... medium in which the target microorganisms grow and are detected by optically sensitive reagents. The reagents change their spectral patterns as the metabolic process takes place. These changes are detected photometrically by an optical instrument and monitored at predetermined time intervals. The ke ...
Plasmid replication and control
... general rule that small plasmids tend to have a high copy number, sometimes over 100 copies per cell, whereas larger plasmids may be present in one or a few copies per cell. Certain classes of plasmids are capable of horizontal transmission from one bacterium to another. In most cases the recipient ...
... general rule that small plasmids tend to have a high copy number, sometimes over 100 copies per cell, whereas larger plasmids may be present in one or a few copies per cell. Certain classes of plasmids are capable of horizontal transmission from one bacterium to another. In most cases the recipient ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... this study are listed in Table 1. The 3′ and 5′ sequences of bovine solute carrier family 5 (sodium/glucose cotransporter) member 1 and 5 (bSGLT1 and bSGLT5) were obtained by rapid amplification of cDNA ends (RACE) using the SMART RACE cDNA amplification kit (Clontech, Palo Alto, CA). The 3′ and 5′ ...
... this study are listed in Table 1. The 3′ and 5′ sequences of bovine solute carrier family 5 (sodium/glucose cotransporter) member 1 and 5 (bSGLT1 and bSGLT5) were obtained by rapid amplification of cDNA ends (RACE) using the SMART RACE cDNA amplification kit (Clontech, Palo Alto, CA). The 3′ and 5′ ...
Supplemental Information
... enriched at least 2-fold in the active nuclease-treated samples compared with the heat-inactivated nuclease-treated samples (Supplementary Figs. 2a and 3a). In addition, 31 species enriched at least 2-fold were observed with masses consistent with known RNA nucleobase modifications (Supplementary Fi ...
... enriched at least 2-fold in the active nuclease-treated samples compared with the heat-inactivated nuclease-treated samples (Supplementary Figs. 2a and 3a). In addition, 31 species enriched at least 2-fold were observed with masses consistent with known RNA nucleobase modifications (Supplementary Fi ...
Two Oxidosqualene Cyclases Responsible for
... synthases after their preferred products or as oxidosqualene cyclases (OSCs) after their common substrate (Abe et al., 1993). The great diversity of triterpenoid structures, with more than 100 different carbon skeletons, is due to different OSCs and, in particular, to the numbers of rearrangement st ...
... synthases after their preferred products or as oxidosqualene cyclases (OSCs) after their common substrate (Abe et al., 1993). The great diversity of triterpenoid structures, with more than 100 different carbon skeletons, is due to different OSCs and, in particular, to the numbers of rearrangement st ...
Virology Journal
... enrichment in log-phase bacterial broth cultures. These three bacteriophages were classified initially within the family Siphoviridae due to their long, non-contractile tails, but their phylogenetic affiliation could not be assessed in the absence of phage genome sequence analysis [8-10]. To date no ...
... enrichment in log-phase bacterial broth cultures. These three bacteriophages were classified initially within the family Siphoviridae due to their long, non-contractile tails, but their phylogenetic affiliation could not be assessed in the absence of phage genome sequence analysis [8-10]. To date no ...
Locked Nucleic Acid (LNA™)
... the primer (Figure 5). Another strategy for LNA™ primer design involves incorporating the LNA™ nucleoside one position away from the 3’-end. Di Guisto and colleagues have found that such primers perform better in proof-reading allele-specific extension (PRASE) PCR experiments. (Di Giusto et al. Nucl ...
... the primer (Figure 5). Another strategy for LNA™ primer design involves incorporating the LNA™ nucleoside one position away from the 3’-end. Di Guisto and colleagues have found that such primers perform better in proof-reading allele-specific extension (PRASE) PCR experiments. (Di Giusto et al. Nucl ...
Planta
... was suggested that isoprene is released by a chemical elimination of diphosphate from dimethylallyl diphosphate (DMADP), a central intermediate in the biosynthesis of isoprenoids, in the thylakoid lumen of the chloroplasts acidi®ed during irradiation (Sanadze 1990). In the meantime, however, an enzy ...
... was suggested that isoprene is released by a chemical elimination of diphosphate from dimethylallyl diphosphate (DMADP), a central intermediate in the biosynthesis of isoprenoids, in the thylakoid lumen of the chloroplasts acidi®ed during irradiation (Sanadze 1990). In the meantime, however, an enzy ...
Translational selection is operative for synonymous codon usage in
... excluding Met, Trp and stop codons), the relative synonymous codon usage (RSCU) (Sharp et al., 1986) and the codon adaptation index (CAI) (Sharp & Li, 1987) were calculated using the program CODONW 1.3 (written by John Peden and available from ftp://molbiol. ox.ac.uk/Win95.codonW.zip). In the two sp ...
... excluding Met, Trp and stop codons), the relative synonymous codon usage (RSCU) (Sharp et al., 1986) and the codon adaptation index (CAI) (Sharp & Li, 1987) were calculated using the program CODONW 1.3 (written by John Peden and available from ftp://molbiol. ox.ac.uk/Win95.codonW.zip). In the two sp ...
Internal expression of Yarrowia NDH2
... the translocation of four protons across the membrane (Brandt, 1999). In mammalian mitochondria, where it is the only NADH dehydrogenase, complex I is composed of 43 subunits with a total molecular mass of nearly 1000 kDa (Walker, 1992; Okun et al., 2000; Sazanov et al., 2000). In fungi such as Neur ...
... the translocation of four protons across the membrane (Brandt, 1999). In mammalian mitochondria, where it is the only NADH dehydrogenase, complex I is composed of 43 subunits with a total molecular mass of nearly 1000 kDa (Walker, 1992; Okun et al., 2000; Sazanov et al., 2000). In fungi such as Neur ...
CLONING AND EXPRESSION OF PLASMIDS ENCODING
... Figure 1 Classification of Antimicrobial Peptides.............................................................. 9 Table 1 Antimicrobial Activity of Indolicidin .................................................................. 11 Figure 2 Gram-Negative Bacterial Cell Wall ........................... ...
... Figure 1 Classification of Antimicrobial Peptides.............................................................. 9 Table 1 Antimicrobial Activity of Indolicidin .................................................................. 11 Figure 2 Gram-Negative Bacterial Cell Wall ........................... ...
CH Zinc Fingers As DNA Binding Domains
... fingers in that it requires an additional, non-zinc finger domain to establish the binding to the target DNA.^^ The other classes, that is the triple, multiple-adjacent and separated-paired C2H2 fingers, bind to the specific DNA sequence without the aid of other domains. Both the triple C2H2 and the ...
... fingers in that it requires an additional, non-zinc finger domain to establish the binding to the target DNA.^^ The other classes, that is the triple, multiple-adjacent and separated-paired C2H2 fingers, bind to the specific DNA sequence without the aid of other domains. Both the triple C2H2 and the ...
FREE Sample Here
... Answer: The three types of eukaryotic microbes are fungi, protozoa, and algae. Because they are all composed of eukaryotic cells, they have basic similarities in cellular structure, including the presence of a nucleus. However, these types of microbes differ in many ways as well. In terms of their n ...
... Answer: The three types of eukaryotic microbes are fungi, protozoa, and algae. Because they are all composed of eukaryotic cells, they have basic similarities in cellular structure, including the presence of a nucleus. However, these types of microbes differ in many ways as well. In terms of their n ...
FREE Sample Here
... Answer: The three types of eukaryotic microbes are fungi, protozoa, and algae. Because they are all composed of eukaryotic cells, they have basic similarities in cellular structure, including the presence of a nucleus. However, these types of microbes differ in many ways as well. In terms of their n ...
... Answer: The three types of eukaryotic microbes are fungi, protozoa, and algae. Because they are all composed of eukaryotic cells, they have basic similarities in cellular structure, including the presence of a nucleus. However, these types of microbes differ in many ways as well. In terms of their n ...
tutorial - ppuigbo
... CAIcal is to copy and paste the codon usage tables from Codon Usage Database (Nakamura et al., 2000). We have therefore added a link to this database in the left frame of the server. The codon usage table from the ‘Codon Usage Database’ format allowed in CAIcal is as follows: ...
... CAIcal is to copy and paste the codon usage tables from Codon Usage Database (Nakamura et al., 2000). We have therefore added a link to this database in the left frame of the server. The codon usage table from the ‘Codon Usage Database’ format allowed in CAIcal is as follows: ...
Title
... http://media.hsls.pitt.edu/media/molbiovideos/metacore1.swf http://media.hsls.pitt.edu/media/molbiovideos/metacore2.swf http://www.hsls.pitt.edu/molbio ...
... http://media.hsls.pitt.edu/media/molbiovideos/metacore1.swf http://media.hsls.pitt.edu/media/molbiovideos/metacore2.swf http://www.hsls.pitt.edu/molbio ...
Alkaline Lonar lake, India – A treasure of
... All the alkaliphilic isolates were tested for the production of extracellular enzymes viz. protease, amylase, lipase and cellulase by growing them in nutrient medium of pH–10 containing casein, starch, tributyrin and carboxy methylcellulose respectively. Lipase from the selected isolate was tested f ...
... All the alkaliphilic isolates were tested for the production of extracellular enzymes viz. protease, amylase, lipase and cellulase by growing them in nutrient medium of pH–10 containing casein, starch, tributyrin and carboxy methylcellulose respectively. Lipase from the selected isolate was tested f ...
biotransformation - University of California, Berkeley
... Nitrosamines are in tobacco smoke, but also can be formed in beer, fish, meats, and chesses that use nitrite as a preservative. ...
... Nitrosamines are in tobacco smoke, but also can be formed in beer, fish, meats, and chesses that use nitrite as a preservative. ...
Bacterial Transformation: Unlocking the Mysteries of Genetic Material
... molecules to make new products or control processes, such as fermentation. Bacteria are the most frequently used living organisms in biotechnology, where they have multiple uses. Although bacteria are often associated with infection, disease and decay, there are many “helpful” bacteria. For example, ...
... molecules to make new products or control processes, such as fermentation. Bacteria are the most frequently used living organisms in biotechnology, where they have multiple uses. Although bacteria are often associated with infection, disease and decay, there are many “helpful” bacteria. For example, ...
Advances in Environmental Biology
... spring temperature-without decreasing it-in pyrex glass containers and high quality flasks, in order to preserve the same temperature. Cultivation in broth medium: The samples were inoculated into selective broth medium of thermophilic Cyanobacteria. Media prepared in this research include BG-11 bro ...
... spring temperature-without decreasing it-in pyrex glass containers and high quality flasks, in order to preserve the same temperature. Cultivation in broth medium: The samples were inoculated into selective broth medium of thermophilic Cyanobacteria. Media prepared in this research include BG-11 bro ...