The Roles of the Quorum-Sensing System in the Release of
... hydrolase (8,9). MVs play a predatory role in the natural ecosystem, as the parent bacteria release them in order to lyse surrounding cells, including both Gram-positive and Gramnegative bacteria. Via this predatory process, MVs help to increase the amount of nutrients available to the parent strain ...
... hydrolase (8,9). MVs play a predatory role in the natural ecosystem, as the parent bacteria release them in order to lyse surrounding cells, including both Gram-positive and Gramnegative bacteria. Via this predatory process, MVs help to increase the amount of nutrients available to the parent strain ...
Characterization of the soil microbial community associated with the
... bacterial counts for each sampling event was determined using the pour plate method. This method included diluting 1 g of soil from each homogenized sample in 99 ml of sterile saline solution (0.9% (w v1) NaCl) and mixed thoroughly. Standard serial dilutions followed and a 1-ml aliquot of each dilu ...
... bacterial counts for each sampling event was determined using the pour plate method. This method included diluting 1 g of soil from each homogenized sample in 99 ml of sterile saline solution (0.9% (w v1) NaCl) and mixed thoroughly. Standard serial dilutions followed and a 1-ml aliquot of each dilu ...
Topologically Non-linked Circular Duplex DNA
... them was broken open. These authors believe that circular DNA is not, in general, topologically helical, but rather has a structure not unlike those proposed by Rodley et al. (1976) and Sasisekharan et al. (1978), in which the two individual single-stranded circular half-chromosomes twist about each ...
... them was broken open. These authors believe that circular DNA is not, in general, topologically helical, but rather has a structure not unlike those proposed by Rodley et al. (1976) and Sasisekharan et al. (1978), in which the two individual single-stranded circular half-chromosomes twist about each ...
DNA Duplication Associated with Charcot-Marie-Tooth Disease Type 1A. Lupski, et al., 1991 Cell, Vol. 66, 219-232, July 26, 1991,
... suggesting a duplication of this locus in CMTlA patients. Genotypes for RMI I-GT for all seven CMTl A pedigrees are shown in Figure 1 and demonstrate that three RMI lGT alleles are present only in affected individuals and are never observed in 53 unaffected offspring and 31 unaffected spouses. The t ...
... suggesting a duplication of this locus in CMTlA patients. Genotypes for RMI I-GT for all seven CMTl A pedigrees are shown in Figure 1 and demonstrate that three RMI lGT alleles are present only in affected individuals and are never observed in 53 unaffected offspring and 31 unaffected spouses. The t ...
Analysis by pulsed-field gel electrophoresis mutations in the
... a/.l 1 in which a non-transposable antibiotic resistance gene cassette is ligated in ilitro to random chromosomal fragments. Following transformation into a host cell, the resistance gene cassette is inserted randomly into the chromosome by means of normal recombinational events with the correspondi ...
... a/.l 1 in which a non-transposable antibiotic resistance gene cassette is ligated in ilitro to random chromosomal fragments. Following transformation into a host cell, the resistance gene cassette is inserted randomly into the chromosome by means of normal recombinational events with the correspondi ...
Polycomb Group silencers collaborate with Notch pathway to cause
... the hsp70-Gal4 genotype crossed with eyeful in the absence of heat shock (—) and after 1h heat-shock (+). PCR amplification of RT lola or psq products was performed from the common region of the lola transcripts (b) or the psq BTB region (lower band in c) and the psq common region (upper bands in c) ...
... the hsp70-Gal4 genotype crossed with eyeful in the absence of heat shock (—) and after 1h heat-shock (+). PCR amplification of RT lola or psq products was performed from the common region of the lola transcripts (b) or the psq BTB region (lower band in c) and the psq common region (upper bands in c) ...
Avian Infectious Bronchitis Virus (IBV) genesig Standard Kit
... significant bench time but also reduces errors. The sensitivity of a one step protocol is also greater than a two step because the entire biological sample is available to the PCR without dilution. This kit will also work well with a two step approach (PrecisionTM nanoScript2 reverse transcription k ...
... significant bench time but also reduces errors. The sensitivity of a one step protocol is also greater than a two step because the entire biological sample is available to the PCR without dilution. This kit will also work well with a two step approach (PrecisionTM nanoScript2 reverse transcription k ...
Back-translation for discovering distant protein homologies
... On leave in J.-V.Poncelet Lab, Moscow, Russia ...
... On leave in J.-V.Poncelet Lab, Moscow, Russia ...
A Major Species of Mouse μ-opioid Receptor mRNA and Its
... 3ⴕ-RACE and DNA Sequencing. Total RNA was extracted from mouse brain as described under Northern Blot Analysis. The 3⬘RACE experiments were performed as described in the protocol of 3⬘-RACE System for rapid amplification of cDNA ends Kit (Invitrogen, Carlsbad, CA). In brief, the first-strand cDNA wa ...
... 3ⴕ-RACE and DNA Sequencing. Total RNA was extracted from mouse brain as described under Northern Blot Analysis. The 3⬘RACE experiments were performed as described in the protocol of 3⬘-RACE System for rapid amplification of cDNA ends Kit (Invitrogen, Carlsbad, CA). In brief, the first-strand cDNA wa ...
Structure-Based Prediction of DNA Target Sites by Regulatory Proteins
... match on complexation. In the case of c-Myb oncoprotein, the flexibility and stability of its DNA-binding domain have been shown to affect the DNA-binding activity.11 The flexibility of DNA is sequence dependent,12 and it can affect the binding affinity with protein as well.13 Given the complexity o ...
... match on complexation. In the case of c-Myb oncoprotein, the flexibility and stability of its DNA-binding domain have been shown to affect the DNA-binding activity.11 The flexibility of DNA is sequence dependent,12 and it can affect the binding affinity with protein as well.13 Given the complexity o ...
Molecular characterization of dioxygenases from polycyclic aromatic
... of 169 amino acids. The Rieske center iron^sulfur binding site [4], CXHRGX8 GNX5 CXZHG, was found to be conserved in all deduced NidA proteins. Also, two histidine residues and one aspartate residue, which according to Parales et al. [6] bind the mononuclear iron, as well as one aspartate residue pr ...
... of 169 amino acids. The Rieske center iron^sulfur binding site [4], CXHRGX8 GNX5 CXZHG, was found to be conserved in all deduced NidA proteins. Also, two histidine residues and one aspartate residue, which according to Parales et al. [6] bind the mononuclear iron, as well as one aspartate residue pr ...
Chromosomal DNA fingerprinting
... BRENDA provides a sensitive means of directly detecting minor genomic differences between micro-organisms. Restriction endonucleases specifically cleave DNA into different lengths, depending on the number and position of the individual recognition sequences, provided that they have not been modified ...
... BRENDA provides a sensitive means of directly detecting minor genomic differences between micro-organisms. Restriction endonucleases specifically cleave DNA into different lengths, depending on the number and position of the individual recognition sequences, provided that they have not been modified ...
Molecular Phylogenetic Analysis Among Bryophytes and
... recovered using GeneCleanII. Every fragment except for the largest was subcloned into pUC18 at the SalI site (pACP3-2 to pACP3-6). Sequencing each subclone, the relative position to the gene was determined and pACP3-3 was further subcloned. pACP3-3 was digested with HindIII and electrophoresed in ag ...
... recovered using GeneCleanII. Every fragment except for the largest was subcloned into pUC18 at the SalI site (pACP3-2 to pACP3-6). Sequencing each subclone, the relative position to the gene was determined and pACP3-3 was further subcloned. pACP3-3 was digested with HindIII and electrophoresed in ag ...
Instructor`s Guide
... will assess their ability to taste the chemical phenylthiocarbamide (PTC) and determine how that ability correlates with their genotype at the TAS2R38 locus, which encodes for a taste receptor expressed in gustatory papillae. There are two common alleles for the TAS2R38 gene, a ‘taster’ allele and a ...
... will assess their ability to taste the chemical phenylthiocarbamide (PTC) and determine how that ability correlates with their genotype at the TAS2R38 locus, which encodes for a taste receptor expressed in gustatory papillae. There are two common alleles for the TAS2R38 gene, a ‘taster’ allele and a ...
Molecular Biology of the Cell
... providing the initial means to identify and number each chromosome. ...
... providing the initial means to identify and number each chromosome. ...
Genetics Test 3 Review Presentation
... location of the phosphate group on a nucleotide • The third carbon of the five carbon sugar has a hydroxyl group (OH). • Nucleotides are linked between the phosphate group at the C-5’ position and the OH group on the C-3’ position. ...
... location of the phosphate group on a nucleotide • The third carbon of the five carbon sugar has a hydroxyl group (OH). • Nucleotides are linked between the phosphate group at the C-5’ position and the OH group on the C-3’ position. ...
... Allosteric effects either increase or decrease the activity of a protein or enzyme by the binding of a ligand (or by protein phosphorylation). The binding cause some sort of change in shape of the protein (+ 4 pt) The enzyme exists in two states relaxed (R) or tense (T) with the relaxed being the ac ...
A novel species of thermoacidophilic archaeon, Sulfolobus
... (Promega). The reverse transcription reaction contained (pl-l): 20 ng RNA template, 0-5 U AMV reverse transcriptase, 1 pmol primer, 5 nmol MgC1, and 1 nmol dNTPs. The reaction was incubated at 42 "C for 30 min. Three independent cDNA products were synthesized and used as templates for the PCR. PCR a ...
... (Promega). The reverse transcription reaction contained (pl-l): 20 ng RNA template, 0-5 U AMV reverse transcriptase, 1 pmol primer, 5 nmol MgC1, and 1 nmol dNTPs. The reaction was incubated at 42 "C for 30 min. Three independent cDNA products were synthesized and used as templates for the PCR. PCR a ...
Recombinant DNA
... If the rDNA sequences encode a gene that is expressed, then the presence of RNA and/or protein products of the recombinant gene can be detected, typically using RTPCR or western hybridization methods.[8] Gross phenotypic changes are not the norm, unless the recombinant gene has been chosen and modi ...
... If the rDNA sequences encode a gene that is expressed, then the presence of RNA and/or protein products of the recombinant gene can be detected, typically using RTPCR or western hybridization methods.[8] Gross phenotypic changes are not the norm, unless the recombinant gene has been chosen and modi ...
Tracking bacterial DNA replication forks in vivo by pulsed field gel
... Cultures were grown for several generations to a cell density of 1 X 108 cells/ml at 37 °C in M9-glucose medium that contained 100 /ug/ml arginine, 50 /ig/ml methionine, 50 /ig/ml tryptophan, 50 /ig/ml threonine, 1 /tg/ml thiamine and 4 /tg/ml thymine. The cells were collected by centrifugation, was ...
... Cultures were grown for several generations to a cell density of 1 X 108 cells/ml at 37 °C in M9-glucose medium that contained 100 /ug/ml arginine, 50 /ig/ml methionine, 50 /ig/ml tryptophan, 50 /ig/ml threonine, 1 /tg/ml thiamine and 4 /tg/ml thymine. The cells were collected by centrifugation, was ...
Construction of an arabidopsis BAC library and isolation of clones
... personal communication). The hybridization probe was made from a 288-bp DNA fragment encoding part of the leucine-rich repeat region, which is a motif found in several recently isolated disease-resistance genes in plants (Staskawicz et al., 1995). We screened the entire BAC library at low stringency ...
... personal communication). The hybridization probe was made from a 288-bp DNA fragment encoding part of the leucine-rich repeat region, which is a motif found in several recently isolated disease-resistance genes in plants (Staskawicz et al., 1995). We screened the entire BAC library at low stringency ...
Mutational Analysis of a Patient with Concomitant
... with CTX. Biochemical analysis shows elevated levels of cholestanol confirming the CTX disease. Interestingly, we also found elevated levels of 7DHC consistent with SLOS suggesting this patient may have a second diagnosis. Our aim is to perform mutational analysis of this patient for the SLOS. I pre ...
... with CTX. Biochemical analysis shows elevated levels of cholestanol confirming the CTX disease. Interestingly, we also found elevated levels of 7DHC consistent with SLOS suggesting this patient may have a second diagnosis. Our aim is to perform mutational analysis of this patient for the SLOS. I pre ...
Io mo0 - Journal of Medical Genetics
... Single stranded conformation polymorphism (SSCP) analysis was carried out on ovine genomic DNA, using primers specific for ovine CFTR intron sequences either side of the exon (ov7i5: GGAAAGTATATAAGCACC and ov7i3: AGAGAGT-lTGCTCATGAC) which corresponds to the human exon 7 sequence. Methods were based ...
... Single stranded conformation polymorphism (SSCP) analysis was carried out on ovine genomic DNA, using primers specific for ovine CFTR intron sequences either side of the exon (ov7i5: GGAAAGTATATAAGCACC and ov7i3: AGAGAGT-lTGCTCATGAC) which corresponds to the human exon 7 sequence. Methods were based ...
Unit 4 Schedule
... The instruction is copied or transcribed from the DNA in the nucleus. This copy is in the form of ribonucleic acid (RNA) in the form of messenger RNA (mRNA), which carries a copy of the genetic instruction from the nucleus to the ribosome. ...
... The instruction is copied or transcribed from the DNA in the nucleus. This copy is in the form of ribonucleic acid (RNA) in the form of messenger RNA (mRNA), which carries a copy of the genetic instruction from the nucleus to the ribosome. ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).