PDF - Biotechnology for Biofuels
... (Fig. 2a, c). Based on the OrthoMCL data with these diatom genomes, the increase in gene content in C. cryptica relative to T. pseudonana is predominantly composed of unique genes not found in any other species, comprising 24% of the gene content. The proportion of unique genes in T. pseudonana was ...
... (Fig. 2a, c). Based on the OrthoMCL data with these diatom genomes, the increase in gene content in C. cryptica relative to T. pseudonana is predominantly composed of unique genes not found in any other species, comprising 24% of the gene content. The proportion of unique genes in T. pseudonana was ...
Chapter 4. The Epigenetics of Non
... key self-renewal or differentiating genes by either directly or indirectly affecting methylation processes. It is also possible for a miRNA to regulate another miRNA. An example of this action is miR-184, which negatively regulates miR-205 in human epithelial cells. Interfering with miR-205 dampens ...
... key self-renewal or differentiating genes by either directly or indirectly affecting methylation processes. It is also possible for a miRNA to regulate another miRNA. An example of this action is miR-184, which negatively regulates miR-205 in human epithelial cells. Interfering with miR-205 dampens ...
HMW glutenin subunits in multiploid Aegilops species: composition
... mobility similar to, or larger than, that of the 1Dx2.2 subunit was observed. However, it was not known if these subunits were encoded by the D genome component in the relevant Aegilops species. ( ) Genomic PCR reactions. Based on the above SDS-PAGE analysis, we chose Ae. ventricosa as a model speci ...
... mobility similar to, or larger than, that of the 1Dx2.2 subunit was observed. However, it was not known if these subunits were encoded by the D genome component in the relevant Aegilops species. ( ) Genomic PCR reactions. Based on the above SDS-PAGE analysis, we chose Ae. ventricosa as a model speci ...
Water at DNA surfaces: Ultrafast dynamics in minor groove recognition
... references therein). X-ray crystallography, NMR, dielectric relaxation, and molecular dynamics simulation studies have shown that a significant amount of water molecules are bound to DNA (for reviews, see refs. 2–6). For example, measurements of dielectric relaxation caused by water molecules bound ...
... references therein). X-ray crystallography, NMR, dielectric relaxation, and molecular dynamics simulation studies have shown that a significant amount of water molecules are bound to DNA (for reviews, see refs. 2–6). For example, measurements of dielectric relaxation caused by water molecules bound ...
Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
... Unit Definition: One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´[32P rA16 into a phosphatase-resistant form in 30 minutes at 37°C Unit Assay Conditions: 1X T4 RNA Ligase reaction buffer, supplemented with 1 mM ATP, is mixed with the RNA substrate (10µM of 5´-[32P]rA1 ...
... Unit Definition: One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´[32P rA16 into a phosphatase-resistant form in 30 minutes at 37°C Unit Assay Conditions: 1X T4 RNA Ligase reaction buffer, supplemented with 1 mM ATP, is mixed with the RNA substrate (10µM of 5´-[32P]rA1 ...
Biology - Unit 8 Teacher Notes DNA and Protein Synthesis
... B.7.4 Explain the process by which a cell copies its DNA and identify factors that can damage DNA and cause changes in its nucleotide sequence. B.7.5 Explain and demonstrate how inserting, substituting or deleting segments of a DNA molecule can alter a gene, which is then passed to every cell that d ...
... B.7.4 Explain the process by which a cell copies its DNA and identify factors that can damage DNA and cause changes in its nucleotide sequence. B.7.5 Explain and demonstrate how inserting, substituting or deleting segments of a DNA molecule can alter a gene, which is then passed to every cell that d ...
PTC Taster Lab Student`s Guide
... will assess their ability to taste the chemical phenylthiocarbamide (PTC) and determine how that ability correlates with their genotype at the TAS2R38 locus, which encodes for a taste receptor expressed in gustatory papillae. There are two common alleles for the TAS2R38 gene, a ‘taster’ allele and a ...
... will assess their ability to taste the chemical phenylthiocarbamide (PTC) and determine how that ability correlates with their genotype at the TAS2R38 locus, which encodes for a taste receptor expressed in gustatory papillae. There are two common alleles for the TAS2R38 gene, a ‘taster’ allele and a ...
3D protein structure
... DeepView. These structures are generated using different techniques, for e.g. x-ray crystallography and published in various journals. All these structures are also available in databases like RCSB protein data bank (see below). In this exercise, we shall look into the p53 protein structure and obse ...
... DeepView. These structures are generated using different techniques, for e.g. x-ray crystallography and published in various journals. All these structures are also available in databases like RCSB protein data bank (see below). In this exercise, we shall look into the p53 protein structure and obse ...
Huntingtin grabs a hammer: DNA repair in HD
... there’s a difference in how normal versus mutant huntingtin moves to the nucleus, or participates in the repair job. Maiuri reasoned that mutant huntingtin could be less mobile, or less efficient. To test this, she worked with normal and mutant skin cells donated by HD patients and their spouses. It ...
... there’s a difference in how normal versus mutant huntingtin moves to the nucleus, or participates in the repair job. Maiuri reasoned that mutant huntingtin could be less mobile, or less efficient. To test this, she worked with normal and mutant skin cells donated by HD patients and their spouses. It ...
Molecular diversity of thermophilic bacteria isolated from Pasinler
... characterization (2). This technique can simply differentiate closely related strains of bacteria, and it can assign bacteria potentially up to the strain level based on the presence of repeated elements within the genome examined (23). Therefore, repPCR was also used for the preliminary screening o ...
... characterization (2). This technique can simply differentiate closely related strains of bacteria, and it can assign bacteria potentially up to the strain level based on the presence of repeated elements within the genome examined (23). Therefore, repPCR was also used for the preliminary screening o ...
Lesson Plan
... Many people can turn the sides of their tongues so that, near the tip, the sides nearly touch on top (Figure 1). When everyone in the class has tried to do this, record the results in Table 1. Also record the data of other class sections and determine the percentages of “rollers” and “nonrollers.” P ...
... Many people can turn the sides of their tongues so that, near the tip, the sides nearly touch on top (Figure 1). When everyone in the class has tried to do this, record the results in Table 1. Also record the data of other class sections and determine the percentages of “rollers” and “nonrollers.” P ...
Abundance of an mRNA is the average number of molecules per cell
... Condensation reaction is one in which a covalent bond is formed with loss of a water molecule, as in the addition of an amino acid to a polypeptide chain. Conditional lethal mlutations kill a cell or virus under certain (nonpermissive) conditions, but allow it to survive under other (permissive) con ...
... Condensation reaction is one in which a covalent bond is formed with loss of a water molecule, as in the addition of an amino acid to a polypeptide chain. Conditional lethal mlutations kill a cell or virus under certain (nonpermissive) conditions, but allow it to survive under other (permissive) con ...
DNA Structure Changes Coupled to Protein Binding
... usually associated with protein binding, but can also be caused by binding of other ligands. It has been shown using various experimental techniques that both intrinsic and protein-induced DNA bending depend on the sequence of nucleotide base pairs (Hagerman, 1990). DNA curvature was initially noted ...
... usually associated with protein binding, but can also be caused by binding of other ligands. It has been shown using various experimental techniques that both intrinsic and protein-induced DNA bending depend on the sequence of nucleotide base pairs (Hagerman, 1990). DNA curvature was initially noted ...
Microbial Ecology: Where are we now?
... Consortium studied the complex microbial communities from various sites of the human body, including the gut, skin and vagina, making use of the Illumina GAIIx platform (The Human Microbiome Project Consortium 2012). Unfortunately, 16S rRNA gene sequencing is not infallible and sometimes exhibits lo ...
... Consortium studied the complex microbial communities from various sites of the human body, including the gut, skin and vagina, making use of the Illumina GAIIx platform (The Human Microbiome Project Consortium 2012). Unfortunately, 16S rRNA gene sequencing is not infallible and sometimes exhibits lo ...
Nonstandard Hydrogen Bonding in Duplex Oligonucleotides. The
... the C1-04 bond in the sugar. It is difficult to imagine an acceptor-donor-donor pyrimidine analog where this electron flow would not be possible. This may explain why this hydrogenbonding scheme is not found in oligonucleotidesfrom organisms that reside on planet earth and is not expected in any DNA ...
... the C1-04 bond in the sugar. It is difficult to imagine an acceptor-donor-donor pyrimidine analog where this electron flow would not be possible. This may explain why this hydrogenbonding scheme is not found in oligonucleotidesfrom organisms that reside on planet earth and is not expected in any DNA ...
Recombination - CCGB | index
... molecules to produce new DNA molecules • Reciprocal recombination: new DNA molecules carry genetic information from both parental molecules. • Gene conversion: one way transfer of information, resulting in an allele on one parental chromosome being changed to the allele from the other homologous chr ...
... molecules to produce new DNA molecules • Reciprocal recombination: new DNA molecules carry genetic information from both parental molecules. • Gene conversion: one way transfer of information, resulting in an allele on one parental chromosome being changed to the allele from the other homologous chr ...
Chpt2_Struc_Nucleic_Acids.doc
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
Investigation of the premelanosome protein
... Including intronic regions as described in Results and discussion. 2Information is related to the different primer pairs: amplified product size (bp)/annealing temperature (°C)/[MgCl2] (mM). For the primers used in the RACE experiments, only the specific PMEL primers are reported (PCR conditions are ...
... Including intronic regions as described in Results and discussion. 2Information is related to the different primer pairs: amplified product size (bp)/annealing temperature (°C)/[MgCl2] (mM). For the primers used in the RACE experiments, only the specific PMEL primers are reported (PCR conditions are ...
Electrophoresis Systems for Nucleic Acids
... When nucleic acid (DNA/RNA) is dissolved in buffer solution, etc. it has a negative charge. When this solution (sample) is added to agarose gel and electrophoresed in buffer solution for a regulated time, the level of migration shown is in accordance with the size of nucleic acid due to the molecula ...
... When nucleic acid (DNA/RNA) is dissolved in buffer solution, etc. it has a negative charge. When this solution (sample) is added to agarose gel and electrophoresed in buffer solution for a regulated time, the level of migration shown is in accordance with the size of nucleic acid due to the molecula ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).