Chromosomes Key - Iowa State University
... 3. When relaxed DNA (10.4 bp/turn) becomes either under or over-coiled it is called what? a) mega-coiled b) coiled-coils c) super-coiled d) ultra-coiled The coiling in question 3 is caused by what type of protein? _topoisomerase___ 4. Prokaryotic chromosomes are different than Eukaryotic chromosomes ...
... 3. When relaxed DNA (10.4 bp/turn) becomes either under or over-coiled it is called what? a) mega-coiled b) coiled-coils c) super-coiled d) ultra-coiled The coiling in question 3 is caused by what type of protein? _topoisomerase___ 4. Prokaryotic chromosomes are different than Eukaryotic chromosomes ...
Introduction to Next Generation Sequencing
... • Moores Law: Advances in technology are driving the ability to address questions on a genomic scale • Optimized Array Design Achievable – Requires Control Spike-In Data for Changes in Assay and Oligo Synthesis Approaches – Time consuming and costly • High Throughput Sequencing (Unbiased Functional ...
... • Moores Law: Advances in technology are driving the ability to address questions on a genomic scale • Optimized Array Design Achievable – Requires Control Spike-In Data for Changes in Assay and Oligo Synthesis Approaches – Time consuming and costly • High Throughput Sequencing (Unbiased Functional ...
DNA technology notes
... are taken from a cell sample, cut out and matched up in pairs • Humans have 23 pairs of chromosomes • Karyotypes can be used to determine if genetic disorder is present • If too many are present can indicate Down’s syndrome • If some are missing can indicate Turner’s syndrome ...
... are taken from a cell sample, cut out and matched up in pairs • Humans have 23 pairs of chromosomes • Karyotypes can be used to determine if genetic disorder is present • If too many are present can indicate Down’s syndrome • If some are missing can indicate Turner’s syndrome ...
Molecular Markers - Personal Web Pages
... May be part of or closely linked to a gene that makes a protein that affects cell survival May be part of controlling elements May be in the larger area of ‘non-coding’ DNA Markers have a known location What is being marked? ...
... May be part of or closely linked to a gene that makes a protein that affects cell survival May be part of controlling elements May be in the larger area of ‘non-coding’ DNA Markers have a known location What is being marked? ...
DNA as Videotape: Introductory Fact Sheet
... take DNA containing one gene from an animal (for example, the gene for insulin from humans) and splice it biologically into the DNA of a bacterium. • That bacterium can multiply, and its offspring will contain the insulin gene. • Those bacteria can make the insulin protein. • DNA from different orga ...
... take DNA containing one gene from an animal (for example, the gene for insulin from humans) and splice it biologically into the DNA of a bacterium. • That bacterium can multiply, and its offspring will contain the insulin gene. • Those bacteria can make the insulin protein. • DNA from different orga ...
forensics - bayo2pisay
... that is repeated in a head-to-tail manner at a specific chromosomal locus interspersed in genome Number of repeated units vary per human Loci – VNTR’s One VNTR – 17 bp, repeated 70 to 450 times Total: 1190 to 7650 base pairs ...
... that is repeated in a head-to-tail manner at a specific chromosomal locus interspersed in genome Number of repeated units vary per human Loci – VNTR’s One VNTR – 17 bp, repeated 70 to 450 times Total: 1190 to 7650 base pairs ...
Trends in Biotechnology
... of PCR, and what researchers do with PCR. 11. Compare the two methods of DNA sequencing: the chemical method and the Sanger method, and know which method is more widely used. How does automation change DNA sequencing? ...
... of PCR, and what researchers do with PCR. 11. Compare the two methods of DNA sequencing: the chemical method and the Sanger method, and know which method is more widely used. How does automation change DNA sequencing? ...
Your name
... 40. Who are Watson and Crick? accredited with the discovery of the structure of DNA 41. What is a dihybrid cross? Genetic test looking at two traits simultaneously ...
... 40. Who are Watson and Crick? accredited with the discovery of the structure of DNA 41. What is a dihybrid cross? Genetic test looking at two traits simultaneously ...
Bioteh_Klonesana un in vivo inhenierija_2015
... a | Nuclease-induced double-strand breaks (DSBs) can lead to sequence insertion, nucleotide correction or change (red box) through homology-directed repair (HDR) in the presence of a donor DNA or a single-strand oligodeoxynucleotide (ssODN), both of which contain homology arms. DSBs can also be repa ...
... a | Nuclease-induced double-strand breaks (DSBs) can lead to sequence insertion, nucleotide correction or change (red box) through homology-directed repair (HDR) in the presence of a donor DNA or a single-strand oligodeoxynucleotide (ssODN), both of which contain homology arms. DSBs can also be repa ...
Study Guide for LS
... A mutagen is something that causes mutations. (Ex: X-rays, U.V. light, radioactivity) We have certain enzymes that repair most DNA mutations. Ultraviolet radiation from the sun is known to cause mutations in skin cells that can lead to cancer, which is why you should wear sunscreen in the summertime ...
... A mutagen is something that causes mutations. (Ex: X-rays, U.V. light, radioactivity) We have certain enzymes that repair most DNA mutations. Ultraviolet radiation from the sun is known to cause mutations in skin cells that can lead to cancer, which is why you should wear sunscreen in the summertime ...
Chapter 11: Organization of DNA in Eukaryotes 11.2: mtDNA
... chloroplasts, allowing these (believed to be) prokaryotes to reside inside of the cytoplasm in a symbiotic relationship. After some time, these mitochondria and chloroplast could no longer live on their own outside of the eukaryote. How large is mtDNA in humans? About 16,000 base pairs, or 16 kilobi ...
... chloroplasts, allowing these (believed to be) prokaryotes to reside inside of the cytoplasm in a symbiotic relationship. After some time, these mitochondria and chloroplast could no longer live on their own outside of the eukaryote. How large is mtDNA in humans? About 16,000 base pairs, or 16 kilobi ...
Electrophoresis literally means “the condition of
... A segment of DNA has two restriction sites–I and II. When incubated with restriction enzymes I and II, three fragments will be formed–a, b, and c. Which of the following gels produced by electrophoresis would represent the separation and identity of these fragments? ...
... A segment of DNA has two restriction sites–I and II. When incubated with restriction enzymes I and II, three fragments will be formed–a, b, and c. Which of the following gels produced by electrophoresis would represent the separation and identity of these fragments? ...
Lecture 23 student powerpoint
... DNA markers are polymorphisms suitable for mapping, used in association with gene markers for genetic and physical mapping of chromosomes. ...
... DNA markers are polymorphisms suitable for mapping, used in association with gene markers for genetic and physical mapping of chromosomes. ...
Exam Review 2B -- Rodermel
... 12. RNA polymerases carry out transcription at a much slower rate than that at which DNA polymerases carry out replication. Why is speed more important in replication than in transcription? ...
... 12. RNA polymerases carry out transcription at a much slower rate than that at which DNA polymerases carry out replication. Why is speed more important in replication than in transcription? ...
Chemistry 5.50 Site Directed Mutagenesis Methods. Site directed
... you know nothing about cloning of genes, use of M13 phage and their life cycle, this is a good place to start. In the next generation of technology associated with making mutants, the success rate of generating the desired mutant was dramatically increased. The methods were developed by Kunkel and E ...
... you know nothing about cloning of genes, use of M13 phage and their life cycle, this is a good place to start. In the next generation of technology associated with making mutants, the success rate of generating the desired mutant was dramatically increased. The methods were developed by Kunkel and E ...
Lesson Plan
... Students view a video describing the process for the lab. Guided Practice: Strawberry DNA Extraction Lab, Students will be given a lab report rubric and the lab report will be due Wednesday/Thursday for a major grade. ...
... Students view a video describing the process for the lab. Guided Practice: Strawberry DNA Extraction Lab, Students will be given a lab report rubric and the lab report will be due Wednesday/Thursday for a major grade. ...
PowerPoint-Präsentation
... of the annotated start of the gene with a maximum distance of ≈1500 nucleotides, indicating that overlap with the promoter site is most likely. A common sequence motif around these CpG sites was not immediately detectable but requires more detailed analysis. ...
... of the annotated start of the gene with a maximum distance of ≈1500 nucleotides, indicating that overlap with the promoter site is most likely. A common sequence motif around these CpG sites was not immediately detectable but requires more detailed analysis. ...
DNA REPLICATION HANDOUT
... 2) Replication Fork: Y-shaped region where new strands of DNA are elongated 3) Okazaki Fragments: Only found on the lagging strand. Since DNA is connected by base pairs, as the original strand “unzips” one of the templates is running in the 5’ to 3’ direction, while the other is 3’ to 5’. As you kno ...
... 2) Replication Fork: Y-shaped region where new strands of DNA are elongated 3) Okazaki Fragments: Only found on the lagging strand. Since DNA is connected by base pairs, as the original strand “unzips” one of the templates is running in the 5’ to 3’ direction, while the other is 3’ to 5’. As you kno ...
Chapter 20: DNA Technology & Genomics
... DNA primers added initiates replication at target sequence DNA polymerase adds nucleotides to primers Cycle is repeated to further amplify DNA sample ...
... DNA primers added initiates replication at target sequence DNA polymerase adds nucleotides to primers Cycle is repeated to further amplify DNA sample ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).