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DNA Barcoding
DNA Barcoding

... is using PCR. The barcode is then sequenced, so that its unique string of As, Cs, Ts, and Gs can be compared to a large database of known barcodes. A database of barcode sequences is hosted by the Barcode of Life Data Systems (BOLD), containing more than four million samples from about 400,000 speci ...
Unit 7 (Molecular Biology - DNA) Study Guide KEY
Unit 7 (Molecular Biology - DNA) Study Guide KEY

... 15. What would be the tRNA for the template above? BONDS WITH MRNA ...
Ch 13 student notes
Ch 13 student notes

... a. Their small size enables millions of organisms to be treated with radiation or chemicals at the same time b. Using this technique, scientists have been able to develop hundreds of beneficial strains, including bacteria that can clean up oil from oil spills 5. New varieties of plants have also bee ...
Core – Practice test 4
Core – Practice test 4

... • The increase in genetic variability. • Why would some grasses be able to grow on ground polluted by an oil spill • Genetic variation within the grass species allows some to survive and reproduce. ...
Principios de Biología Molecular
Principios de Biología Molecular

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What is a pedigree?

Mutation and DNA Repair
Mutation and DNA Repair

... Another chemical instability is that cytosine occasionally gets deaminated: it loses an amino group. This converts it into uracil, which is not a DNA base and is removed by repair enzymes. However, in many places, a C followed by a G (CpG: the “p” is the connecting phosphate) gets methylated: a CH3 ...
DNA Fingerprinting: The Code to Identification
DNA Fingerprinting: The Code to Identification

... come from different sources. However, it is possible to misinterpret the statistics. A statement that there is a tiny probability of finding a match if the accused is innocent is not the same as stating that the probability of the accused being innocent is just as minute. For example, if the probab ...
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BIOTECHNOLOGY - Bishop Amat Memorial High School

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03-131 Genes, Drugs, and Diseases Exam 2 – F2015 Name:____________________
03-131 Genes, Drugs, and Diseases Exam 2 – F2015 Name:____________________

... bonding Temperature is raised to the optimal temperature for polymerase, polymerase synthesizes a copy of the template by adding bases to the primer. Choice B: ddNTP is a di-deoxy nucleotide tri phosphate. It has three phosphates on its 5’ carbon so it can be incorporated into the growing DNA, but s ...
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... Single ringed N Bases = Pyrimidines are C and T 2X ringed N Bases = Purines are A and G Scientists Watson and Crick proposed structure of DNA as a ____________, held together by H Bonds and __________ These complementary bases were A bonded w/ ____ G bonded w/ _______ II. DNA REPLICATION What is it? ...
SEMESTER 2 Toxicology/Drug Testing
SEMESTER 2 Toxicology/Drug Testing

... o How can the angle of impact be determined from a blood droplet? o Given a blood stain, be able to determine both directionality and angle of impact. o Know the proper preservation of blood and semen stains. ...
Origins of Sugars in the Prebiotic World
Origins of Sugars in the Prebiotic World

MATCH
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... l. ___ -10 and -35 promoters m. ___ TATA box and CAAT box promoters n. __ co translation o.___ no nucleus p. ___ bacterial cells q.___ polymerase requires nucleotides r. ___ 5’ -> 3’ polymerases s. ___ many transcription factors, some tissue specific i. ___ plant and animal cells t._____ circular ch ...
2012_Protein_Regionals_Exam
2012_Protein_Regionals_Exam

Epigenetics - Cayetano Heredia University
Epigenetics - Cayetano Heredia University

... Allele-specific Methylation • CpG (5'-C-G-3') occurs less frequently in the mammalian genome than any other dinucleotide. • CpG islands are clusters of CpG sites near the 5' ends of genes and are the target of DNA methylation. ...
Diapositive 1 - ac-aix
Diapositive 1 - ac-aix

... coloring specific to the DNA consolidated us in our idea that the collected ball was well of the DNA. ...
protein synthesis - Ms. Dooley`s Science Class
protein synthesis - Ms. Dooley`s Science Class

... PROTEIN SYNTHESIS This activity will help you become more familiar with the process of protein synthesis and will help distinguish between transcription and translation. Use your book to help review any problems. PART 1 - Transcription During transcription, the DNA double helix “unzips”. As the hydr ...
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Biology

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Classwork May 15th

... DNA Structure (chapter 19) 1. Describe the structure of DNA. What are the sides composed of? What are the rungs composed of? [3pts] 2. State the rule of complimentary base pairing. [2pts] 3. What is the full name for the bases ATGC? [4pts] 4. Describe the purpose and process of DNA replication (be s ...
Key
Key

... A. can synthesize a polymer. B. can begin synthesis without a primer. C. can add nucleotides to a 3’ end. D. does not require the strands to separate. E. uses a DNA strand as a template. 5. In eukaryotic cells, the DNA strand that serves as a template for transcription is determined by A. the origin ...
Practical II - Faculty Websites
Practical II - Faculty Websites

... 10. (351) The following figure shows an agarose gel. Three fragments of DNA (A, B, and C) have been loaded into wells toward the top of the gel, and the positive pole of the electric field is at the bottom. The sizes of the three DNA fragments are indicated below. (a) After a defined period of migra ...
Protein Synthesis
Protein Synthesis

Gene Copy Number analysis using semi
Gene Copy Number analysis using semi

... DNA loci,, using g a limited number of cycles y to allow accurate quantitation in the exponential amplification range. Each multiplex PCR will yield a pattern composed p p of fluorescent peaks, p with each p peak corresponding p g to a specific p DNA locus. The comparison of fluorescence is done bet ...
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Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
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