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4/3
4/3

... • Covalent attachment ...
One of the best ways to get the full benefit of your supplements
One of the best ways to get the full benefit of your supplements

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Proteins are biopolymers construced from similar building blocks
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Carbs, Lipids, and Proteins
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Key to Exam 2
Key to Exam 2

... conjunction with gel electrophoresis to identify specific proteins or subunits recognized by antibodies. Many times the two methods will provide the same information about a protein and can be used interchangeably. In other situations one of the methods will work better or be more appropriate. For e ...
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Bimolecular fluorescence complementation



Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.
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