n - IBIVU
... separately from GARt in yeast, and in bacteria each domain is encoded separately (Henikoff et al., ...
... separately from GARt in yeast, and in bacteria each domain is encoded separately (Henikoff et al., ...
Gene Section AFF3 (lymphoid nuclear protein related to AF4)
... Coding sequence of LAF4 compared to AF4 and site of fusion. Schematic representation of MLL, LAF4, AF4, and the putative MLL-LAF4 fusion protein. Domains in MLL are shaded: MT, DNA methyltransferase homology region; TRX, Drosophila trithorax homology. The percentage of amino acid homology between co ...
... Coding sequence of LAF4 compared to AF4 and site of fusion. Schematic representation of MLL, LAF4, AF4, and the putative MLL-LAF4 fusion protein. Domains in MLL are shaded: MT, DNA methyltransferase homology region; TRX, Drosophila trithorax homology. The percentage of amino acid homology between co ...
Protein structure - Primary
... Tertiary Structure • This refers to the 3 dimensional folding of the chain. This structure can be globular or fibrous. The shapes give certain properties to the protein • Globular : In these the protein chain is rolled up like a ball of wool. This structure makes the protein soluble. This type of p ...
... Tertiary Structure • This refers to the 3 dimensional folding of the chain. This structure can be globular or fibrous. The shapes give certain properties to the protein • Globular : In these the protein chain is rolled up like a ball of wool. This structure makes the protein soluble. This type of p ...
PSIpred
... Inspite of large potential and high content of protein in fenugreek seeds, however, no reports on molecular structure predictions is available on Trigonella spp. native to this region. ...
... Inspite of large potential and high content of protein in fenugreek seeds, however, no reports on molecular structure predictions is available on Trigonella spp. native to this region. ...
Protein structure
... • Group of residues with high contact density, number of contacts within domains is higher than the number of contacts between domains. • A stable unit of protein structure that can fold autonomously • A rigid body linked to other domains by flexible linkers. • A portion of the protein that can be a ...
... • Group of residues with high contact density, number of contacts within domains is higher than the number of contacts between domains. • A stable unit of protein structure that can fold autonomously • A rigid body linked to other domains by flexible linkers. • A portion of the protein that can be a ...
Workshop VIII Fungal Cell Factories Chair: Cees van den Hondel 183
... Metabolic engineering in filamentous fungi is very promising since many fungi produce high value secondary metabolites (e.g. antibiotics). Ribozyme technology as a tool for metabolic engineering can be used in order to influence metabolic pathways more broadly than other methods available such as mu ...
... Metabolic engineering in filamentous fungi is very promising since many fungi produce high value secondary metabolites (e.g. antibiotics). Ribozyme technology as a tool for metabolic engineering can be used in order to influence metabolic pathways more broadly than other methods available such as mu ...
PowerPoint
... the hydrodynamic properties of proteins so that you understand why things work the way they do ...
... the hydrodynamic properties of proteins so that you understand why things work the way they do ...
Purified Mouse Anti-p115 — 612260
... through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. p115 is a 959 amino acid protein located at ...
... through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. p115 is a 959 amino acid protein located at ...
Protein modification in eukaryotic cell-free systems
... modifications and cofactors. A huge number of approaches which can be summarized as general ligation techniques and chemical aminoacylation of tRNAs, have been pursued to address this limitation given by the natural repertoire to generate proteins with enhanced or novel properties. Overcoming genera ...
... modifications and cofactors. A huge number of approaches which can be summarized as general ligation techniques and chemical aminoacylation of tRNAs, have been pursued to address this limitation given by the natural repertoire to generate proteins with enhanced or novel properties. Overcoming genera ...
Fundamentals of protein stability
... Over the years various models have been proposed that liken the protein interior to an oil drop or a molecular crystal. This is not a question of semantics only, since the answer could be decisive in choosing thermodynamic model reactions for transfer of amino acid side chains. Depending on the phys ...
... Over the years various models have been proposed that liken the protein interior to an oil drop or a molecular crystal. This is not a question of semantics only, since the answer could be decisive in choosing thermodynamic model reactions for transfer of amino acid side chains. Depending on the phys ...
3 types of protein transport
... which allows lateral diffusion within the ERmembrane of both the ER-signal sequence and trans-membrane domains ...
... which allows lateral diffusion within the ERmembrane of both the ER-signal sequence and trans-membrane domains ...
P8010Datasheet-Lot0921211
... the active site. In a reaction containing 20 µg/ml Factor Xa, 2 µM dansyl-Glu-Gly-Arg-chloromethyl ketone will inactivate > 95% of the Factor Xa in 1 minute at room temperature. ...
... the active site. In a reaction containing 20 µg/ml Factor Xa, 2 µM dansyl-Glu-Gly-Arg-chloromethyl ketone will inactivate > 95% of the Factor Xa in 1 minute at room temperature. ...
Supplementary Information
... filaggrin domains induces DNA degradation. (A) Western blot analysis of FLG-N expressed in keratinocytes. ...
... filaggrin domains induces DNA degradation. (A) Western blot analysis of FLG-N expressed in keratinocytes. ...
Structure Determination and Sequence Analysis - Rose
... The residue side-chains tend to be flexible, and can move freely. This is especially true for surface residues; however, even side-chains within the protein interior may be able to move relatively freely. The backbone also has some degree of flexibility. Most proteins “breathe”: the structure transi ...
... The residue side-chains tend to be flexible, and can move freely. This is especially true for surface residues; however, even side-chains within the protein interior may be able to move relatively freely. The backbone also has some degree of flexibility. Most proteins “breathe”: the structure transi ...
Nickel affinity chromatography in Protein purification
... Nitrilotriacetic acid (NTA), Iminodiacetic acid (IDA) both could be used to purify proteins with histidine molecules. NTA coordinates the Ni2+ with four valences and two valences are available for interaction with imidazole rings of histidine. ...
... Nitrilotriacetic acid (NTA), Iminodiacetic acid (IDA) both could be used to purify proteins with histidine molecules. NTA coordinates the Ni2+ with four valences and two valences are available for interaction with imidazole rings of histidine. ...
Protein Expression and Purification
... • Identify domains, select domains for expression • e.g. kinase domain from RTKs for assays and structure based drug discovery • Fusion tags ? – Which host cell system? – Which expression vector? ...
... • Identify domains, select domains for expression • e.g. kinase domain from RTKs for assays and structure based drug discovery • Fusion tags ? – Which host cell system? – Which expression vector? ...
Functions and inhibitors of SecA, an essential protein in bacterial
... across cytoplasmic membranes. In addition to the high-affinity SecA-SecYEG-SecDF•YajC protein-conducting channels, we have found that there are low-affinity SecA-only channels that elicit ion channel activity and promote protein translocation. These pore channels are less efficient, and like Prl sup ...
... across cytoplasmic membranes. In addition to the high-affinity SecA-SecYEG-SecDF•YajC protein-conducting channels, we have found that there are low-affinity SecA-only channels that elicit ion channel activity and promote protein translocation. These pore channels are less efficient, and like Prl sup ...
6. 3-D structure of proteins
... • The spatial arrangement of atoms in a protein is called its conformation. • Proteins in any of their functional folded conformations are called native proteins. • Stability can be defined as the tendency to ...
... • The spatial arrangement of atoms in a protein is called its conformation. • Proteins in any of their functional folded conformations are called native proteins. • Stability can be defined as the tendency to ...
A human phenome-interactome network of protein complexes
... Yves Moreau & Søren Brunak Nature Biotechnology 25, 309 - 316 (2007) ...
... Yves Moreau & Søren Brunak Nature Biotechnology 25, 309 - 316 (2007) ...
Proteins_Fats
... Importance of proteins • Protein is important for everyone, regardless of age or activity level. Protein is best known for its ability to build and maintain lean body mass. It also maintains strong hair, skin, and teeth. But it doesn’t stop there – protein is also vitally important in maintaining b ...
... Importance of proteins • Protein is important for everyone, regardless of age or activity level. Protein is best known for its ability to build and maintain lean body mass. It also maintains strong hair, skin, and teeth. But it doesn’t stop there – protein is also vitally important in maintaining b ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.