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RNA PP
RNA PP

... to DNA and separates the DNA strands. RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA. • So, RNA is making a single-stranded copy from DNA that takes information out of the nucleus. ...
DNA Structure and Replication
DNA Structure and Replication

... 14. This type of replication is called semi-conservative replication. Considering the meaning of these words (semi—half; conserve—to keep), explain why DNA replication is called semi-conservative. ...
What is a chromosome?
What is a chromosome?

... Without histones, the unwound DNA in chromosomes would be very long (a length to width ratio of more than 10 million to 1 in human DNA). For example, each human cell has about 1.8 meters of DNA, but wound on the histones it has about 90 micrometers (0.09 mm) of chromatin, which, when duplicated and ...
Review Sheet for Test #1
Review Sheet for Test #1

... Each species has a specific number of chromosomes in the _______________ of each of its cells. For example, humans have ______ chromosomes in each body cell, and dogs have _____ in each body cell. There is a tongue fern that has _________ in each body cell! The number of chromosomes that an organism ...
APDNA 2015 16
APDNA 2015 16

... Primase starts an RNA chain from scratch - adds RNA nucleotides one at a time using the parental DNA as a template - 3 end serves as the starting point for the new DNA strand ...
Chromosomes
Chromosomes

... a normal female; 46,XY, a normal male; and 45,X (or sometimes 45,XO) an individual who has only one X chromosome, a condition that produces Turner’s syndrome. Karyotypes followed by a plus and then a number indicate trisomy, the inheritance of a whole extra chromosome. For example, 47,XX,+21 denotes ...
DNA Replication Simulation WKST
DNA Replication Simulation WKST

... Now you are ready to start adding new DNA nucleotides using the old strands. Before DNA replication can start, a primer must be added so DNA polymerase can bind. A primer is a short stretch of RNA that initiates DNA replication by allowing polymerase to bind and add nucleotides to the end of it. Pri ...
Chapter 16
Chapter 16

... The usual replication machinery provides no way to complete the 5 ends, so repeated rounds of replication produce shorter DNA molecules with uneven ends ...
D.N.A. activity
D.N.A. activity

... a) the length of the total DNA, uncoiled and laid end-to-end, within one human somatic cell. b) The length of the entire amount of DNA in their body (again, assume uncoiled and laid out). c) The closest astronomical object to that distance. d) OPTIONAL: The total number of trips to the sun and back ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
A conserved N-‐terminal domain of Rif2 regulates telomere length in

... The  regulation  of  telomere  length  equilibrium  is  essential  for  cell  growth  and   survival  since  critically  short  telomeres  signal  DNA  damage  and  cell  cycle  arrest.   While  the  broad  principles  of  length  regulatio ...
E. coli
E. coli

... mainly of b and other half made primarily of b’ •Channel between two parts of the claw is presumably where DNA passes through •Three Asp residues at active site coordinate Mg2+ and are critical for catalysis ...
DNA Replication
DNA Replication

... A always pairs with T and G always pairs with C the pairs of bases are held in place by hydrogen attractions (bonds) (3 bonds between G-C and two between A-T) ...
Bio 102 Practice Problems
Bio 102 Practice Problems

... 2. DNA polymerase cannot start synthesis without a primer; because of this limitation, a cell's DNA actually contains some short stretches of RNA. 3. Watson and Crick’s DNA structure was especially convincing because it immediately suggested a hypothesis for how a nucleotide sequence could be transl ...
7.014 Problem Set 3 Solutions
7.014 Problem Set 3 Solutions

... i. Model A: DNA is a double-stranded helix with sugar-phosphate backbones in the center, and bases sticking out into solution. In this model, the strands are running anti-parallel to each other. This model should be rejected. DNA backbone is an alternating sugar-phosphate polymer. Phosphates are ne ...
7.014 Problem Set 3 Solutions
7.014 Problem Set 3 Solutions

... i. Model A: DNA is a double-stranded helix with sugar-phosphate backbones in the center, and bases sticking out into solution. In this model, the strands are running anti-parallel to each other. This model should be rejected. DNA backbone is an alternating sugar-phosphate polymer. Phosphates are neg ...
replicates
replicates

... Cells function according to the information contained in the master code of DNA (i.e., cell cycle, DNA to DNA, and DNA to RNA). ...
Chapter 16.
Chapter 16.

...  stabilized by single-stranded binding proteins ...
Chapter 17_part 2
Chapter 17_part 2

... introduce either single-or double strand breaks in DNA. Once the supercoiling is relaxed, the broken strands are joined together and the tropoisomerase diffuses from the location of the replication fork. ...
CHAPTER 12
CHAPTER 12

... • Staining mitotic chromosomes can provide useful information. • A karyotype is a preparation of homologous pairs ordered according to size. • The pattern on a karyotype may be used to screen chromosomal abnormalities. ...
Cell Cycle DNA Structure and Replication Student PPT Nts
Cell Cycle DNA Structure and Replication Student PPT Nts

... • ______________________: when a chunk of DNA (usually large) is removed from 1 chromosome and attached to another ...
DNAandReplication Exam Qs.doc
DNAandReplication Exam Qs.doc

... showing a lack of understanding of the structure of DNA. In part (ii), X and Y were usually correct, but Z was sometimes named as uracil. Examiners were amazed at the number of spelling variants offered for thymine. E4.(a) This proved to be a good discriminator. Most students were aware that DNA hel ...
supplementary material
supplementary material

... Figure S1 Telomeres remain stable and capped in i-TERT mice. a, Northern blot analysis showing induction of TERT in i-TERT MEFs treated with doxycycline for 72 hours (left) or splenocytes treated with doxycycline for 48 hours (right) as compared with controls. b, Representative metaphases from i-TER ...
Chromosome Chromo
Chromosome Chromo

... cells are arrested in mitosis, and are then “harvested” for chromosome analysis After harvesting, the cell preparations are dropped onto glass slides and stained. For most chromosome analyses, a G-banding technique is utilized for staining. Metaphase spread ...
No Slide Title
No Slide Title

... to a selectable marker and transform only cells which took up plasmid containing marker and origin grew call eukaryotic origins ARS ...
Level 3 - rgreenbergscience
Level 3 - rgreenbergscience

... on how to build the proteins in every living organism, what proteins are needed to create the organism, and in which sequence amino acids are needed in order to build all of the different proteins required for the organism’s growth. Structure: All DNA (in humans, snakes, frogs, birds, turtles, insec ...
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Telomere



A telomere is a region of repetitive nucleotide sequences at each end of a chromatid, which protects the end of the chromosome from deterioration or from fusion with neighboring chromosomes. Its name is derived from the Greek nouns telos (τέλος) 'end' and merοs (μέρος, root: μερ-) 'part.' For vertebrates, the sequence of nucleotides in telomeres is TTAGGG. This sequence of TTAGGG is repeated approximately 2,500 times in humans. During chromosome replication, the enzymes that duplicate DNA cannot continue their duplication all the way to the end of a chromosome, so in each duplication the end of the chromosome is shortened (this is because the synthesis of Okazaki fragments requires RNA primers attaching ahead on the lagging strand). The telomeres are disposable buffers at the ends of chromosomes which are truncated during cell division; their presence protects the genes before them on the chromosome from being truncated instead.Over time, due to each cell division, the telomere ends become shorter. They are replenished by an enzyme, telomerase reverse transcriptase.
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