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Dna Deoxyribonucleic acid - Bethlehem Catholic High School
Dna Deoxyribonucleic acid - Bethlehem Catholic High School

...  The other strand of DNA, called the lagging strand, elongates away from the replication fork.  The lagging strand is synthesized discontinuously into small segments, called Okazaki fragments. ...
Nature Rev.Mol.Cell Biol
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... Replicative polymerase encounters DNA damage on template strand ...
The Search for the Genetic Material
The Search for the Genetic Material

... • 1. DNA helicase unwinds the DNA at the replication forks. • -leading strand (3’-5’), replicates (5’-3’) towards the fork. 2. Molecules of single strand binding protein prevent the DNA from sticking back together. 3. Primase synthesizes an RNA primer at the end of 5’end. 4. DNA pol III synthesizes ...
The Search for the Genetic Material
The Search for the Genetic Material

... • 1. DNA helicase unwinds the DNA at the replication forks. • -leading strand (3’-5’), replicates (5’-3’) towards the fork. 2. Molecules of single strand binding protein prevent the DNA from sticking back together. 3. Primase synthesizes an RNA primer at the end of 5’end. 4. DNA pol III synthesizes ...
DNA and Mitosis - Birmingham City Schools
DNA and Mitosis - Birmingham City Schools

... fragments on the lagging strand are called Okazaki fragments after the Japanese Biochemist Reiji Okazaki. ...
DNA extraction from cheek cells protocol I mailed to you
DNA extraction from cheek cells protocol I mailed to you

... 6. Complete the following sentences to describe the structure of DNA. In the backbone of each strand in the DNA double helix molecule, the sugar of one nucleotide is bonded to the __________________ in the next nucleotide. The ________________ of the nucleotides in each strand of DNA extend toward e ...
DNA Structure and Replication
DNA Structure and Replication

... Unique site in prokaryotes - ori C in E. coli, handout Multiple sites in eukaryotes fig. 11.14 Rules for polymerization of Nucleic acids (N.A): fig 11.15 a. N.A. are produced by copying preexisting DNA strand (complementary base pairing) b. Growth is ONLY from 5’->3’. Energy is provided by NTPs (rib ...
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... were made using different fragments of DNA taken from O. sativa indica. The plants were then submerged for a period of 11 days. The heights of all the plants were measured at the beginning and at the end of the submergence period. ...
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... • HOW DO YOU THINK SCIENTISTS WERE ABLE TO DETERMINE THAT DNA WAS THE INHERITANCE MOLECULE THAT WAS PASSED FROM PARENTS TO OFFSPRING? (12.1) ...
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... serve as a guide in producing tRNA and rRNA  Some regulate gene expression, such as enhancers or silencers  Introns are segments of DNA within a gene, but not included in the final polypeptide product ...
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Unit 4: DNA: Our Genetic Material Notes

... Unit 4: DNA: Our Genetic Material Notes ...
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National Human Genome Research Institute

... specific instructions that make each type of living creature unique. The term chromosome comes from the Greek words for color (chroma) and body (soma). Scientists gave this name to chromosomes because they are cell structures, or bodies, that are strongly stained by some colorful dyes used in resear ...
Note: all of these sentences are true.
Note: all of these sentences are true.

... 37.Telomeres are repetitive non coding DNA sequences plus protein at the end of linear chromosomes. That is preventing attack by nucleases. 38.In eukaryote, a telomere is a region of RNA primers attach further on the DNA strand and DNA polymerase comes along and continues to make a new DNA. ...
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...  The packaging of DNA into chromosomes involves several orders of DNA coiling and folding.  The normal human karyotype is made up of 46 chromosomes consisting of 22 pairs of autosomes and a pair of sex chromosomes, XX in the female, and XY in the male.  Each chromosome consists of a short (p) and ...
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... dle of other genes and disrupt them. For instance, jumping genes could disrupt pigment genes in corn cells, leading to spotted kernels. • McClintock's jumping genes are now called transposons. • Current evidence suggests that all organisms, including humans, have tr ...
iGenetics: A Molecular Approach DNA: The Genetic Material
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... 48) Describe the packing of chromatin from the 10-nm to the 30-nm fiber stage. What is the role of histones? Answer: 10-nm chromatin fiber consists of nucleosomes–"beads" of DNA wound around eight core histone proteins–connected by strands of linker DNA. The 30-nm chromatin fiber is created by the b ...
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... Telomerase activity maintains the terminal DNA repeats Telomerase binding proteins (TRFs) bind single and double stranded telomerase repeats TRFs 1. Protect against DNA repair 2. End-joining of chromosomes 3. Spurious exonuclease activity ...
DNA Structure and Replication
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... 2. The two single strands of DNA then each serve as a template for a new stand to be created. Using DNA as a template means that on the new strand, the bases are placed in the correct order because of the base pairing rules. As a template strand is read, the new strand is created. If ATGCCA is on th ...
Mechanisms of cell aging in cell culture
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... due to old age or due to terminal differentiation (e.g. nerve cells). It is assumed that the aging of cells also occurs in vivo and that the life expectancy and replicative lifetime of cells are under common genetic control. There are suggestions that the aging of cells prevents the formation of tum ...
DNA extraction from cheek cells protocol I mailed to you
DNA extraction from cheek cells protocol I mailed to you

... 6. Complete the following sentences to describe the structure of DNA. In the backbone of each strand in the DNA double helix molecule, the sugar of one nucleotide is bonded to the __________________ in the next nucleotide. The ________________ of the nucleotides in each strand of DNA extend toward e ...
Comparing DNA and RNA
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... Like DNA, ribonucleic acid (RNA) is a nucleic acid— a molecule made of nucleotides linked together, RNA differs from DNA in three ways, First, RNA consists of a single strand of nucleotides instead of the two strands found in DNA. Second, RNA nucleotides contain the five-carbon sugar ribose rather t ...
DNA Fingerprinting
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... -G-C-T- T-C-C-A-G-C-G-A-A-G-G-T-CIdentify “primer sequences” and design primers. Add DNA + primers + nucleotides (G,A,T,C) + DNA polymerase. Heat DNA (separate the strands) Cool DNA (primers anneal and DNA polymerase assembles new strand) 1 CYCLE = two complete identical copies of DNA ...
DNA  1. Evidence for DNA as the genetic material.
DNA 1. Evidence for DNA as the genetic material.

... chromosomes this is not possible (because there is not a previous nucleotide) on the lagging strand. ii. Each time the cell divides, one strand of DNA cannot be completely replicated so a single-stranded section remains. Enzymes remove these single-stranded sections. iii. As a result, the chromosome ...
Chapter 25
Chapter 25

... • RNAPI promoters: - are species specific, each RNAPI recognizes a specific promoter. - are located -186 to +6 on the DNA template strand. • RNAPII promoters: - The constitutive genes have GC box (GGGCGG consensus sequence) in their promoters - The structural genes have TATA box (TATATAATA sequence) ...
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Telomere



A telomere is a region of repetitive nucleotide sequences at each end of a chromatid, which protects the end of the chromosome from deterioration or from fusion with neighboring chromosomes. Its name is derived from the Greek nouns telos (τέλος) 'end' and merοs (μέρος, root: μερ-) 'part.' For vertebrates, the sequence of nucleotides in telomeres is TTAGGG. This sequence of TTAGGG is repeated approximately 2,500 times in humans. During chromosome replication, the enzymes that duplicate DNA cannot continue their duplication all the way to the end of a chromosome, so in each duplication the end of the chromosome is shortened (this is because the synthesis of Okazaki fragments requires RNA primers attaching ahead on the lagging strand). The telomeres are disposable buffers at the ends of chromosomes which are truncated during cell division; their presence protects the genes before them on the chromosome from being truncated instead.Over time, due to each cell division, the telomere ends become shorter. They are replenished by an enzyme, telomerase reverse transcriptase.
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