Supplementary Notes S1 (doc 64K)
... At least 1 probe was selected for 10,675 (97%) of the total 11,000 regions. 96% of regions have 6 or more probes. Most regions are ~1200 bp in size and typically have 25-35 probes. Larger regions may have as many as 62 probes. ...
... At least 1 probe was selected for 10,675 (97%) of the total 11,000 regions. 96% of regions have 6 or more probes. Most regions are ~1200 bp in size and typically have 25-35 probes. Larger regions may have as many as 62 probes. ...
Comprehensive Analysis of Hyrdrogen Bonds in Regulatory Protein
... of these bonds involve the protein side-chains and the DNA atoms at the base edges and in the backbone, but interactions that involve the protein backbone are also found. The contacts that involve the DNA backbone are believed to stabilize the complex and to orient the protein against the DNA in a f ...
... of these bonds involve the protein side-chains and the DNA atoms at the base edges and in the backbone, but interactions that involve the protein backbone are also found. The contacts that involve the DNA backbone are believed to stabilize the complex and to orient the protein against the DNA in a f ...
Diversity of DNA methyltransferases that recognize asymmetric
... and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecules in the asymmetric unit represent a biologically relevant dimer. In M.AhdI, two copies of the AhdIS subunit ...
... and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecules in the asymmetric unit represent a biologically relevant dimer. In M.AhdI, two copies of the AhdIS subunit ...
Chapter 16 The Molecular Basis of Inheritance Multiple
... 5) After mixing a heat-killed, phosphorescent strain of bacteria with a living non-phosphorescent strain, you discover that some of the living cells are now phosphorescent. Which observations would provide the best evidence that the ability to fluoresce is a heritable trait? A) DNA passed from the ...
... 5) After mixing a heat-killed, phosphorescent strain of bacteria with a living non-phosphorescent strain, you discover that some of the living cells are now phosphorescent. Which observations would provide the best evidence that the ability to fluoresce is a heritable trait? A) DNA passed from the ...
GBSA: a comprehensive software for analysing whole genome
... study DNA methylation at nucleotide resolution. Although several programmes provide genomewide alignment of BS-treated reads, the resulting information is not readily interpretable and often requires further bioinformatic steps for meaningful analysis. Current post-alignment BS-sequencing programmes ...
... study DNA methylation at nucleotide resolution. Although several programmes provide genomewide alignment of BS-treated reads, the resulting information is not readily interpretable and often requires further bioinformatic steps for meaningful analysis. Current post-alignment BS-sequencing programmes ...
The Role of DNA Structure and Dynamics in the
... bound with E2. Although E2 does not contact the N4 spacer, the affinities are modulated by the base composition of this DNA part. Nevertheless, the origin of either the global recognition mechanism or the spacer effect remains unclear, particularly in the case of the bovine papillomavirus type 1 E2 ...
... bound with E2. Although E2 does not contact the N4 spacer, the affinities are modulated by the base composition of this DNA part. Nevertheless, the origin of either the global recognition mechanism or the spacer effect remains unclear, particularly in the case of the bovine papillomavirus type 1 E2 ...
Nucleotide Sequence of the Osmoregulatory proU Operon of
... The inferred amino acid sequence of ProV shows significant similarity in two regions to HisP, a component of the L-histidine transporter of S. typhimurium (24) (Fig. 4). These same regions of HisP are in turn known to be homologous with corresponding regions in one component of each of the other bin ...
... The inferred amino acid sequence of ProV shows significant similarity in two regions to HisP, a component of the L-histidine transporter of S. typhimurium (24) (Fig. 4). These same regions of HisP are in turn known to be homologous with corresponding regions in one component of each of the other bin ...
Raven/Johnson Biology 8e Chapter 17 Answers 1. A recombinant
... A. Answer a is incorrect. Restriction endonucleases are used to break up DNA. They are not part of the polymerase chain reaction. The correct answer is d— B. Answer b is incorrect. PCR generates DNA fragments, not RNA. The correct answer is d— C. Answer c is incorrect. Reverse transcriptase is used ...
... A. Answer a is incorrect. Restriction endonucleases are used to break up DNA. They are not part of the polymerase chain reaction. The correct answer is d— B. Answer b is incorrect. PCR generates DNA fragments, not RNA. The correct answer is d— C. Answer c is incorrect. Reverse transcriptase is used ...
repetitive extragenic palindromic sequences in pseudomonas
... following negatively oriented REP sequences shared another sequence Considering the conserved palindromy of REP sequences and the conserved arrangement of the clusters it is probable that REP sequences adopt conformations with peculiar secondary structures especially suitable to be specifically reco ...
... following negatively oriented REP sequences shared another sequence Considering the conserved palindromy of REP sequences and the conserved arrangement of the clusters it is probable that REP sequences adopt conformations with peculiar secondary structures especially suitable to be specifically reco ...
Journal of Molecular Biology
... tested. phoR strain CE1220 was transformed with pJY50 and. in caont.rast to thcb parental phoR strain, all 25 CmR transformants t)ested did not produce alkaline phosphatase constit,utively and were resistant to phage T(‘45. Therefore ~~JP50 also carries the phoR gene. In order to determine a restric ...
... tested. phoR strain CE1220 was transformed with pJY50 and. in caont.rast to thcb parental phoR strain, all 25 CmR transformants t)ested did not produce alkaline phosphatase constit,utively and were resistant to phage T(‘45. Therefore ~~JP50 also carries the phoR gene. In order to determine a restric ...
Red Line Walk-through
... sequences that appear to come from the same transcription locus (gene or expressed pseudogene), together with information on protein similarities, gene expression, cDNA clone reagents, and genomic location.” http://www.ncbi.nlm.nih.gov/u nigene cDNA: DNA produced by reverse transcribing mRNA using r ...
... sequences that appear to come from the same transcription locus (gene or expressed pseudogene), together with information on protein similarities, gene expression, cDNA clone reagents, and genomic location.” http://www.ncbi.nlm.nih.gov/u nigene cDNA: DNA produced by reverse transcribing mRNA using r ...
RTS™ pIVEX E. coli His-tag 2nd Generation Vector Set Manual
... containing the appropriate restriction sites is prepared, cloning into different pIVEX vectors can be done easily in parallel or successively. To minimize problems, biotechrabbit recommends selecting the cloning strategy strictly according to Table 1. For cloning strategies allowing the minimization ...
... containing the appropriate restriction sites is prepared, cloning into different pIVEX vectors can be done easily in parallel or successively. To minimize problems, biotechrabbit recommends selecting the cloning strategy strictly according to Table 1. For cloning strategies allowing the minimization ...
MHF1 plays Fanconi anaemia complementation group M protein
... parallel pathways of HR suppression in Arabidopsis. Hyperrecombination in the fancm but not the recq4A mutant can be abolished by MHF1 mutations. This finding indicates that MHF1 and FANCM act at different steps of a single, common, HR pathway. Keywords: MHF1, Fanconi anaemia complementation group M ...
... parallel pathways of HR suppression in Arabidopsis. Hyperrecombination in the fancm but not the recq4A mutant can be abolished by MHF1 mutations. This finding indicates that MHF1 and FANCM act at different steps of a single, common, HR pathway. Keywords: MHF1, Fanconi anaemia complementation group M ...
Principles of Genetic Variation
... population genetics that relate to the spread of advantageous DNA variants through human populations (but the population genetics of harmful disease-associated DNA variants is examined in Chapter 5). Genetic variation is most highly developed in genes that work in recognizing foreign, potentially ha ...
... population genetics that relate to the spread of advantageous DNA variants through human populations (but the population genetics of harmful disease-associated DNA variants is examined in Chapter 5). Genetic variation is most highly developed in genes that work in recognizing foreign, potentially ha ...
PPT - Glasnost
... # The 'slices' program - slicing arrays. @sequences = ( 'TTATTATGTT', 'GCTCAGTTCT', 'GACCTCTTAA', 'CTATGCGGTA', 'ATCTGACCTC' ); print "@sequences\n"; @seq_slice = @sequences[ 1 .. 3 ]; print "@seq_slice\n"; print "@sequences\n"; @removed = splice @sequences, 1, 3; print "@sequences\n"; print "@remov ...
... # The 'slices' program - slicing arrays. @sequences = ( 'TTATTATGTT', 'GCTCAGTTCT', 'GACCTCTTAA', 'CTATGCGGTA', 'ATCTGACCTC' ); print "@sequences\n"; @seq_slice = @sequences[ 1 .. 3 ]; print "@seq_slice\n"; print "@sequences\n"; @removed = splice @sequences, 1, 3; print "@sequences\n"; print "@remov ...
Real-time Quantification of HER2/neu Gene Amplification by
... The techniques used to evaluate the HER2/neu gene status have included gene-based assays such as Southern and slot blotting, in-situ hybridization (fluorescent and nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast p ...
... The techniques used to evaluate the HER2/neu gene status have included gene-based assays such as Southern and slot blotting, in-situ hybridization (fluorescent and nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast p ...
August 4–5, 2016 Fred Hutchinson Cancer Research Center Seattle
... evidence that HIV RNA is detected in many latently infected cells and that some HIV proteins/peptides may be expressed. The high rate of indirect killing of T cells during all phases of HIV suggests that HIV antigens may be displayed on such cells; even among persons with optimal clinical and virolo ...
... evidence that HIV RNA is detected in many latently infected cells and that some HIV proteins/peptides may be expressed. The high rate of indirect killing of T cells during all phases of HIV suggests that HIV antigens may be displayed on such cells; even among persons with optimal clinical and virolo ...
Programmed Materials Synthesis with DNA
... Seeman and co-workers recently developed a solidsupport methodology for preparing DNA materials to help overcome the problems (i.e., low yield, purification) associated with their solution-based methods.54 With this approach, the yields were significantly increased and more complex structures that c ...
... Seeman and co-workers recently developed a solidsupport methodology for preparing DNA materials to help overcome the problems (i.e., low yield, purification) associated with their solution-based methods.54 With this approach, the yields were significantly increased and more complex structures that c ...
CfE Higher Biology Unit 1: DNA and the Genome
... of phosphorus and sulfur, would confirm DNA as the genetic material and eliminated protein as the carrier of genetic information. Edwin Chargaff, using paper chromatography and ultraviolet spectroscopy techniques, demonstrated two findings, now known as Chargaff’s rules: firstly, that adenine and th ...
... of phosphorus and sulfur, would confirm DNA as the genetic material and eliminated protein as the carrier of genetic information. Edwin Chargaff, using paper chromatography and ultraviolet spectroscopy techniques, demonstrated two findings, now known as Chargaff’s rules: firstly, that adenine and th ...
Prevention of DNA Rereplication Through a Meiotic Recombination
... kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks are generated but not repaired due to absence of DMC1, a pathway involving the checkpoint gene RAD17 prevents this DNA rereplication. Further genetic analysis has now reveale ...
... kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks are generated but not repaired due to absence of DMC1, a pathway involving the checkpoint gene RAD17 prevents this DNA rereplication. Further genetic analysis has now reveale ...
Screening of a Specific Point Mutation in Tumor Suppressor p53
... immortalize primary rat embryo fibroblast cells and cooperate with an activated ras oncogene to transform cells. They have transforming activity and are transdominant over wild-type p53. The trans-dominant phenotype of the mutated p53 protein may be explained by its ability to oligomerize with wild- ...
... immortalize primary rat embryo fibroblast cells and cooperate with an activated ras oncogene to transform cells. They have transforming activity and are transdominant over wild-type p53. The trans-dominant phenotype of the mutated p53 protein may be explained by its ability to oligomerize with wild- ...
64$ CfE Higher Biology Unit 1: DNA and the
... of phosphorus and sulfur, would confirm DNA as the genetic material and eliminated protein as the carrier of genetic information. Edwin Chargaff, using paper chromatography and ultraviolet spectroscopy techniques, demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and th ...
... of phosphorus and sulfur, would confirm DNA as the genetic material and eliminated protein as the carrier of genetic information. Edwin Chargaff, using paper chromatography and ultraviolet spectroscopy techniques, demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and th ...
64$ CfE Higher Biology Unit 1: DNA and the
... of phosphorus and sulfur, would confirm DNA as the genetic material and eliminated protein as the carrier of genetic information. Edwin Chargaff, using paper chromatography and ultraviolet spectroscopy techniques, demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and th ...
... of phosphorus and sulfur, would confirm DNA as the genetic material and eliminated protein as the carrier of genetic information. Edwin Chargaff, using paper chromatography and ultraviolet spectroscopy techniques, demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and th ...