Tutorial - Ensembl
... An Ensembl gene may have multiple transcripts reflecting alternative splicing, in this case the EPO gene only shows one transcript in Ensembl. ...
... An Ensembl gene may have multiple transcripts reflecting alternative splicing, in this case the EPO gene only shows one transcript in Ensembl. ...
D-loop - BioMed Central
... and a negative AT-skew (-0.2531), i.e. the transcript of the major strand is relatively rich in nucleotides G and T, and correspondently poor in C and A (nucleotide frequencies as follows: T 0.447, C 0.110, A 0.267, G 0.176). This pattern, referred to as the reverse strand bias [1], is also found in ...
... and a negative AT-skew (-0.2531), i.e. the transcript of the major strand is relatively rich in nucleotides G and T, and correspondently poor in C and A (nucleotide frequencies as follows: T 0.447, C 0.110, A 0.267, G 0.176). This pattern, referred to as the reverse strand bias [1], is also found in ...
A Dnmt2-like protein mediates DNA methylation in
... (unmethylated, negative control) and Drosophila embryos were spotted onto a membrane and stained for 5methylcytosine and DNA, respectively. This revealed a clearly differential staining pattern for 5-methylcytosine, while the DNA staining pattern was equal for all samples (Fig. 1E). As an additional ...
... (unmethylated, negative control) and Drosophila embryos were spotted onto a membrane and stained for 5methylcytosine and DNA, respectively. This revealed a clearly differential staining pattern for 5-methylcytosine, while the DNA staining pattern was equal for all samples (Fig. 1E). As an additional ...
PDF - 1.9 MB
... breeding fly would you want to mate to in order to carry out a three-factor cross with b, pr, and vg? (b) For the cross described in (a) what type of strain would be the best to mate to in order to score the gamete genotypes passed to the next generation? (c) What would be the rarest phenotypic clas ...
... breeding fly would you want to mate to in order to carry out a three-factor cross with b, pr, and vg? (b) For the cross described in (a) what type of strain would be the best to mate to in order to score the gamete genotypes passed to the next generation? (c) What would be the rarest phenotypic clas ...
Seven Zinc Finger transcription factors are novel regulators of the
... Fig. S2. Alignment of conserved DNA-binding domains of the Zn Finger TFs. (A) Alignment of the C2H2-type Zn Finger TFs identified in this study with the Arabidopsis TFs described by Sakamoto et al. (2004). In that study, those TFs were shown to bind to the cis-element A(G/C)T-X3-4-A(G/C)T. For ZOS12 ...
... Fig. S2. Alignment of conserved DNA-binding domains of the Zn Finger TFs. (A) Alignment of the C2H2-type Zn Finger TFs identified in this study with the Arabidopsis TFs described by Sakamoto et al. (2004). In that study, those TFs were shown to bind to the cis-element A(G/C)T-X3-4-A(G/C)T. For ZOS12 ...
Molecular cloning, characterization, and homologous
... °C for 3 min. The final extension was performed for 10 min at 72 °C. Reaction mixtures were run in a 0.8% agarose gel. PCR products were extracted from the gel and ligated to pGEM-T Easy Vector (Promega). Ligation product was then transferred into the competent E. coli DH5α cells, which were prepare ...
... °C for 3 min. The final extension was performed for 10 min at 72 °C. Reaction mixtures were run in a 0.8% agarose gel. PCR products were extracted from the gel and ligated to pGEM-T Easy Vector (Promega). Ligation product was then transferred into the competent E. coli DH5α cells, which were prepare ...
DNA is the genetic material
... Hershey/Chase Experiments• They grew one batch of T2 phage in the presence of radioactive sulfur, marking the proteins (but not the DNA) radioactive. • They grew another batch in the presence of radioactive phosphorus, marking the DNA (but not the proteins) radioactive. • They allowed each batch to ...
... Hershey/Chase Experiments• They grew one batch of T2 phage in the presence of radioactive sulfur, marking the proteins (but not the DNA) radioactive. • They grew another batch in the presence of radioactive phosphorus, marking the DNA (but not the proteins) radioactive. • They allowed each batch to ...
hiv in uganda - Makerere University News Portal
... in basic science research in HIV where little basic research has been done in Africa To establish a basic HIV/AIDS research centre in Africa in future. To accelerate basic reseach to deveop new and more effective antiretroviral drugs that are affordable to HIV/AIDS patients. ...
... in basic science research in HIV where little basic research has been done in Africa To establish a basic HIV/AIDS research centre in Africa in future. To accelerate basic reseach to deveop new and more effective antiretroviral drugs that are affordable to HIV/AIDS patients. ...
Complete Genomics Sample Quality Control Protocol
... Inspecting Gel Images Use the gel images to verify that the DNA for each sample is of high molecular weight and is double stranded. Examine the gel profile for each sample to verify the presence of a single band above the location of the 20 KB band of the GeneRuler™ 1 KB Plus DNA Ladder (see Figure ...
... Inspecting Gel Images Use the gel images to verify that the DNA for each sample is of high molecular weight and is double stranded. Examine the gel profile for each sample to verify the presence of a single band above the location of the 20 KB band of the GeneRuler™ 1 KB Plus DNA Ladder (see Figure ...
Mutation Analysis of the Ornithine Transcarbamylase (OTC) Gene in
... two known and three novel mutations of the OTC gene in five Japanese patients including two neonatal-onset, one late-onset, and two symptomatic female patients. Known nonsense mutations (c.578G>A and c.421C>T) were detected in a neonatal-onset male and a symptomatic female patient, respectively. Mut ...
... two known and three novel mutations of the OTC gene in five Japanese patients including two neonatal-onset, one late-onset, and two symptomatic female patients. Known nonsense mutations (c.578G>A and c.421C>T) were detected in a neonatal-onset male and a symptomatic female patient, respectively. Mut ...
Nucleotide Sequence of the SAC2 Gene of Saccharomyces cerevisiae .
... the 4.3 kb ClaI-SaZI fragment, which is equivalent sequence contained an open reading frame with to the insert in pRK4, into the integrating vector the capacity to code for a 74kDa hydrophilic YIPS. The 8.5 kb BglII-PvuII fragment was iso- protein and part of another reading frame (Figure lated afte ...
... the 4.3 kb ClaI-SaZI fragment, which is equivalent sequence contained an open reading frame with to the insert in pRK4, into the integrating vector the capacity to code for a 74kDa hydrophilic YIPS. The 8.5 kb BglII-PvuII fragment was iso- protein and part of another reading frame (Figure lated afte ...
Use of Recombinant Adenovirus for Metabolic Engineering of
... these cells precludes the use of retroviruses for efficient gene transfer. Furthermore, retrovirus-mediated gene therapy for metabolic disorders can only be efficiently applied to tissues that can be surgically resected, manipulated in uitro, and replaced; these procedures may be viable for liver (W ...
... these cells precludes the use of retroviruses for efficient gene transfer. Furthermore, retrovirus-mediated gene therapy for metabolic disorders can only be efficiently applied to tissues that can be surgically resected, manipulated in uitro, and replaced; these procedures may be viable for liver (W ...
Site-Directed Mutagenesis Using Oligonucleotide
... distinct lengths. For genetic engineering in the E. coli chromosome, two efficient λ Redmediated methods were developed by two independent groups. The first method utilized E. coli strains containing the PL operon of a defective λ prophage (e.g., deletion of cro to bioA genes, which includes the lyt ...
... distinct lengths. For genetic engineering in the E. coli chromosome, two efficient λ Redmediated methods were developed by two independent groups. The first method utilized E. coli strains containing the PL operon of a defective λ prophage (e.g., deletion of cro to bioA genes, which includes the lyt ...
Detecting a Transposon in Corn
... Thus, McClintock explained color variegations, such as speckled kernels, that had intrigued botanists for centuries. (See Concept 32 of DNA From the Beginning, www.dnaftb.org, for an animation explaining McClintock’s elucidation of the Ac/Ds system.) Today the Ac/Ds system is an important tool in ge ...
... Thus, McClintock explained color variegations, such as speckled kernels, that had intrigued botanists for centuries. (See Concept 32 of DNA From the Beginning, www.dnaftb.org, for an animation explaining McClintock’s elucidation of the Ac/Ds system.) Today the Ac/Ds system is an important tool in ge ...
Package `rDNA`
... can be specified. The vertex types or groups of the row labels in the network.matrix can be submitted as a one-column matrix or data.frame with meaningful row names. The row and column names of the network.matrix are looked up in the partitions if specified. Alternatively, partitions can be given as ...
... can be specified. The vertex types or groups of the row labels in the network.matrix can be submitted as a one-column matrix or data.frame with meaningful row names. The row and column names of the network.matrix are looked up in the partitions if specified. Alternatively, partitions can be given as ...
Adaptive value of sex in microbial pathogens
... chance effects in finite populations as the main sources of genetic associations that may provide an advantage for sex (Otto and Gerstein, 2006; Peters and Otto, 2003). According to this view, advantageous mutations occurring on advantageous backgrounds will sweep through the population. Disadvantag ...
... chance effects in finite populations as the main sources of genetic associations that may provide an advantage for sex (Otto and Gerstein, 2006; Peters and Otto, 2003). According to this view, advantageous mutations occurring on advantageous backgrounds will sweep through the population. Disadvantag ...
Detecting HFE Mutations in Human Genomic DNA
... which the body accumulates too much iron, leading to iron overload. In 1996 two mutations were identified in the HFE gene, that when present in the homozygote or compound heterozygote, can lead to the presentation of the disease. The HFE gene is located on the short (p) arm of Chromosome 6. The gene ...
... which the body accumulates too much iron, leading to iron overload. In 1996 two mutations were identified in the HFE gene, that when present in the homozygote or compound heterozygote, can lead to the presentation of the disease. The HFE gene is located on the short (p) arm of Chromosome 6. The gene ...
DNA Testing - Who Murdered Robert Wone
... communicate the true, chain-reaction nature of PCR. In PCR, the original DNA is copied, then the copies are copied, those copies are copied and so on. This results in dramatic increases in the amount of DNA that couldn't be easily accomplished in the Xeroxing analogy. The PCR process deserves its cl ...
... communicate the true, chain-reaction nature of PCR. In PCR, the original DNA is copied, then the copies are copied, those copies are copied and so on. This results in dramatic increases in the amount of DNA that couldn't be easily accomplished in the Xeroxing analogy. The PCR process deserves its cl ...
Cutting Edge: DNA Polymerases and Are Dispensable for Ig Gene
... mutations introduced in Ig genes involve the participation of an error-prone DNA polymerase (reviewed in Ref. 2). The specific characteristics of these mutations appear indeed poorly compatible with other types of DNA-modifying activities, as, for example, chemical alterations or base modifications. ...
... mutations introduced in Ig genes involve the participation of an error-prone DNA polymerase (reviewed in Ref. 2). The specific characteristics of these mutations appear indeed poorly compatible with other types of DNA-modifying activities, as, for example, chemical alterations or base modifications. ...
A novel DNA modification by sulphur
... normal (Zhou et al., 1988; Boybek et al., 1998; Dyson and Evans, 1998) and pulsed-field gel electrophoresis (Kieser et al., 1992; Zhou et al., 1994). This phenotype is named Dnd (for DNA degradation). Such double-stranded scission at modification sites was proved to be a peracidmediated, oxidative a ...
... normal (Zhou et al., 1988; Boybek et al., 1998; Dyson and Evans, 1998) and pulsed-field gel electrophoresis (Kieser et al., 1992; Zhou et al., 1994). This phenotype is named Dnd (for DNA degradation). Such double-stranded scission at modification sites was proved to be a peracidmediated, oxidative a ...
S4 Text.
... enzyme. Contamination of an enzyme with DNA or another enzyme can be costly and timeconsuming. Work as quickly as possible so that the enzyme is out of the freezer for as short a time as possible. If using the enzyme at your bench, keep it on ice or in a benchtop cooler at all times. Return enzyme t ...
... enzyme. Contamination of an enzyme with DNA or another enzyme can be costly and timeconsuming. Work as quickly as possible so that the enzyme is out of the freezer for as short a time as possible. If using the enzyme at your bench, keep it on ice or in a benchtop cooler at all times. Return enzyme t ...
Laboratory 4 Patterns of Inheritance (human)
... development—the ring fingers of boys and men are typically longer than their index fingers while in girls and women these fingers are usually the same or the index finger is slightly longer. More background information is in Appendix B The shorter index finger to ring finger may also be a sex-influe ...
... development—the ring fingers of boys and men are typically longer than their index fingers while in girls and women these fingers are usually the same or the index finger is slightly longer. More background information is in Appendix B The shorter index finger to ring finger may also be a sex-influe ...
A systematic search for DNA methyltransferase polymorphisms
... DNMT1 gene. A total number of 25 different single nucleotide polymorphisms (SNPs) and a small fraction of deletions of an ‘AG’ dinucleotide at intron 21 were detected (Supplementary Material, Table S1). Three of these SNPs were found to induce amino acid changes (Table 1 and Fig. 1). The first one w ...
... DNMT1 gene. A total number of 25 different single nucleotide polymorphisms (SNPs) and a small fraction of deletions of an ‘AG’ dinucleotide at intron 21 were detected (Supplementary Material, Table S1). Three of these SNPs were found to induce amino acid changes (Table 1 and Fig. 1). The first one w ...
bbr038online 474..484 - Oxford Academic
... conducted even by individual laboratories. However, this will yield more and more genome sequences that are not well assembled, and will hinder thorough annotation when no closely related reference genome is available. One of the challenging issues is the identification of protein-coding sequences s ...
... conducted even by individual laboratories. However, this will yield more and more genome sequences that are not well assembled, and will hinder thorough annotation when no closely related reference genome is available. One of the challenging issues is the identification of protein-coding sequences s ...
ppt
... Infinite Alleles Model (Crow and Kimura Model) Each mutation creates a completely new allele Alleles are lost by drift and gained by mutation: a balance occurs Is this realistic? Average human protein contains about 300 amino acids (900 nucleotides) Number of possible mutant forms of a gene: ...
... Infinite Alleles Model (Crow and Kimura Model) Each mutation creates a completely new allele Alleles are lost by drift and gained by mutation: a balance occurs Is this realistic? Average human protein contains about 300 amino acids (900 nucleotides) Number of possible mutant forms of a gene: ...