Plasmids
... URA3 gene, which allows ura3 mutants like BY4742, the parent strain of our mutants, to grow in the absence of uracil after they are transformed with plasmids (Chapter 12). Promoters control transcription of plasmid sequences ...
... URA3 gene, which allows ura3 mutants like BY4742, the parent strain of our mutants, to grow in the absence of uracil after they are transformed with plasmids (Chapter 12). Promoters control transcription of plasmid sequences ...
Structural Features of Angiotensin-I Converting Enzyme Catalytic Sites
... peptidases family [23,24] (Fig. (2)). An attempt to classify previously unknown biopolymers with distinct motifs and zinc-binding affinity at the beginning of the 90’s led researchers to identify diagnostic motifs within the polypeptide amino acid sequences through sequence and topology comparison [ ...
... peptidases family [23,24] (Fig. (2)). An attempt to classify previously unknown biopolymers with distinct motifs and zinc-binding affinity at the beginning of the 90’s led researchers to identify diagnostic motifs within the polypeptide amino acid sequences through sequence and topology comparison [ ...
Heredity in Fingerprints
... The chance distribution of genes during reduction and the resulting variation in characters of offspring makes one person different from another. The particular combination of genes in one person will never occur again, and has not occurred before. Genes can be carried without indicating their prese ...
... The chance distribution of genes during reduction and the resulting variation in characters of offspring makes one person different from another. The particular combination of genes in one person will never occur again, and has not occurred before. Genes can be carried without indicating their prese ...
Human Pif1 helicase is a G-quadruplex DNA
... by full-length hPif1 and the core helicase domain hPifHD. With ss/dsDNA substrates, such as PST55, the unwinding activity of full-length hPif1 increased steeply from little or no unwinding at 1 nM to a peak at 2 nM and decreasing thereafter. The reason why this activity profile is observed is unclea ...
... by full-length hPif1 and the core helicase domain hPifHD. With ss/dsDNA substrates, such as PST55, the unwinding activity of full-length hPif1 increased steeply from little or no unwinding at 1 nM to a peak at 2 nM and decreasing thereafter. The reason why this activity profile is observed is unclea ...
DNA - IPN-Kiel
... (ii) make copies of itself so that this information can be passed on during cell division and from generation to generation In recent years, because of our increasing knowledge and understanding of DNA and its related molecule RNA, it has been possible to work with this genetic information and to us ...
... (ii) make copies of itself so that this information can be passed on during cell division and from generation to generation In recent years, because of our increasing knowledge and understanding of DNA and its related molecule RNA, it has been possible to work with this genetic information and to us ...
Mutations
... crossing-over g results in a deletion on one homolog and a duplication on the other homolog ...
... crossing-over g results in a deletion on one homolog and a duplication on the other homolog ...
Evolutionary significance of stress- induced mutagenesis in
... selective cost associated with the activity of error-free DNA repair systems. Therefore, there is no strong selective pressure to reduce the error-rate. Another example of molecular constraints leading to mutagenesis is RpoS-dependent induction of the dinB gene, which is specific to the stationary p ...
... selective cost associated with the activity of error-free DNA repair systems. Therefore, there is no strong selective pressure to reduce the error-rate. Another example of molecular constraints leading to mutagenesis is RpoS-dependent induction of the dinB gene, which is specific to the stationary p ...
Genetic Markers for Sex Identification in Forensic DNA Analysis
... defect known as amelogenesis imperfecta, which results in the abnormal formation of tooth enamel [3]. The AMEL locus has two homologous genes: AMELX, which is located on the distal short arm of the X chromosome in the p22.1-p22.3 region (Figure 1; Table 1), and AMELY, located near the centromere of ...
... defect known as amelogenesis imperfecta, which results in the abnormal formation of tooth enamel [3]. The AMEL locus has two homologous genes: AMELX, which is located on the distal short arm of the X chromosome in the p22.1-p22.3 region (Figure 1; Table 1), and AMELY, located near the centromere of ...
A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is
... with 7 and 3 tandem zinc finger repeats, CtBP-binding motifs, and other possible transcriptional regulatory domains. In this study, we here identify 3 transcription start sites of MEL1 mRNA and 2 kinds of MEL1 gene products; one is the full-length MEL1 protein with a PR domain, and the other is a sh ...
... with 7 and 3 tandem zinc finger repeats, CtBP-binding motifs, and other possible transcriptional regulatory domains. In this study, we here identify 3 transcription start sites of MEL1 mRNA and 2 kinds of MEL1 gene products; one is the full-length MEL1 protein with a PR domain, and the other is a sh ...
Physical and Topological Properties of Circular DNA
... at room temperature. Examples of such molecules are the DNA's from the papova group of tumor viruses and certain intracellular forms of OX and X-DNA. These DNA's have several common properties which derive from the topological requirement that the winding number in such molecules is invariant. They ...
... at room temperature. Examples of such molecules are the DNA's from the papova group of tumor viruses and certain intracellular forms of OX and X-DNA. These DNA's have several common properties which derive from the topological requirement that the winding number in such molecules is invariant. They ...
Identification of a cis-Element That Determines Autonomous DNA
... In Vitro DNA Replication—The cell-free replication assay was adapted from the method described previously (11) and as performed previously (18). The earliest labeled fragment method was performed using the in vitro DNA replication system, as described previously (11, 18). In brief, the in vitro reac ...
... In Vitro DNA Replication—The cell-free replication assay was adapted from the method described previously (11) and as performed previously (18). The earliest labeled fragment method was performed using the in vitro DNA replication system, as described previously (11, 18). In brief, the in vitro reac ...
MD Simulations of the P53 oncoprotein structure
... Considering the above noted difficulty of zinc binding in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bonded approaches are used to descr ...
... Considering the above noted difficulty of zinc binding in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bonded approaches are used to descr ...
DNA damage in round spermatids of mice with a targeted disruption
... oocytes. We have investigated a mouse model of azoospermia for quality of haploid germ cell genomes, based on 4,6-diamidino-2-phenylindole (DAPI)/TdT-mediated dUTP nick-end labelling (TUNEL) labelling. The mouse model, a targeted mutation in the protein phosphatase 1cg gene, results in severe deplet ...
... oocytes. We have investigated a mouse model of azoospermia for quality of haploid germ cell genomes, based on 4,6-diamidino-2-phenylindole (DAPI)/TdT-mediated dUTP nick-end labelling (TUNEL) labelling. The mouse model, a targeted mutation in the protein phosphatase 1cg gene, results in severe deplet ...
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE
... proportion of their mtDNA in either the form of catenanes in which monomer circles are intermeshed Iike links in a chain, or as simple head to tail unicircular dimers (Clayton, 1982). Evidence for recombination between molecules has been lacking (discussed in Brown, 1985; Moritz et al., 1987). Howev ...
... proportion of their mtDNA in either the form of catenanes in which monomer circles are intermeshed Iike links in a chain, or as simple head to tail unicircular dimers (Clayton, 1982). Evidence for recombination between molecules has been lacking (discussed in Brown, 1985; Moritz et al., 1987). Howev ...
Evolution of antifreeze glycoprotein gene from a trypsinogen gene in
... teleosts, whose equilibrium freezing point (20.7 to 218C) is significantly higher than that of seawater (21.98C), by adsorbing to small ice crystals in the body and inhibiting their growth (6–9). Besides AFGPs, there are three other structurally different types of antifreeze proteins from various po ...
... teleosts, whose equilibrium freezing point (20.7 to 218C) is significantly higher than that of seawater (21.98C), by adsorbing to small ice crystals in the body and inhibiting their growth (6–9). Besides AFGPs, there are three other structurally different types of antifreeze proteins from various po ...
Soliton-like base pair opening in a helicoidal DNA: An analogy with
... nucleotides known as strands with linkage interms of dipole-dipole interaction along the strands and the two strands are coupled to each other through hydrogen bonds between the complementary bases [1]. Molecular excitations in DNA based on the above model is generally governed by nonlinear evolutio ...
... nucleotides known as strands with linkage interms of dipole-dipole interaction along the strands and the two strands are coupled to each other through hydrogen bonds between the complementary bases [1]. Molecular excitations in DNA based on the above model is generally governed by nonlinear evolutio ...
Assembly of additional heterochromatin distinct from centromere
... the ‘neocentromere’, forms and is maintained in the complete absence of alphoid DNA (Saffery et al., 2003). However, introduced alphoid yeast artificial chromosome (YAC) and/or bacterial artificial chromosome (BAC) DNA generates stable HACs in some transfected cell lines, whereas in the remainder of ...
... the ‘neocentromere’, forms and is maintained in the complete absence of alphoid DNA (Saffery et al., 2003). However, introduced alphoid yeast artificial chromosome (YAC) and/or bacterial artificial chromosome (BAC) DNA generates stable HACs in some transfected cell lines, whereas in the remainder of ...
Honor Genetics DNA structure and replication
... Matthew Meselson and Franklin Stahl cultured E. coli bacteria for several generations on a medium containing nucleotide precursors labeled with a heavy isotope of nitrogen, 15N. The bacteria incorporated the heavy nitrogen into their DNA. The scientists then transferred the bacteria to a medium with ...
... Matthew Meselson and Franklin Stahl cultured E. coli bacteria for several generations on a medium containing nucleotide precursors labeled with a heavy isotope of nitrogen, 15N. The bacteria incorporated the heavy nitrogen into their DNA. The scientists then transferred the bacteria to a medium with ...
Microsynth GmbH
... but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it would do with the target sequence. Nevertheless, a binding and subsequent extension is poss ...
... but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it would do with the target sequence. Nevertheless, a binding and subsequent extension is poss ...
Rabbit genome editing with zinc finger nucleases
... targeted Zinc ?nger nuclease and, optionally, at least one accessory polynucleotide. The method further comprises incubating the embryo or cell to alloW expression of the Zinc ?nger nuclease, Wherein a double-stranded break introduced into the targeted chromosomal sequence by the Zinc ?nger nuclease ...
... targeted Zinc ?nger nuclease and, optionally, at least one accessory polynucleotide. The method further comprises incubating the embryo or cell to alloW expression of the Zinc ?nger nuclease, Wherein a double-stranded break introduced into the targeted chromosomal sequence by the Zinc ?nger nuclease ...
Mechanisms of fast and stringent search in homologous pairing of
... PH, and shear force, suggesting that it may serve as the ‘default’ mode of chromosome pairing in vivo [2]. Various models have been proposed to explain the homology-dependent attraction between dsDNA molecules [9–11], many of which attribute this interaction to hydrophobic forces or electrostatics. ...
... PH, and shear force, suggesting that it may serve as the ‘default’ mode of chromosome pairing in vivo [2]. Various models have been proposed to explain the homology-dependent attraction between dsDNA molecules [9–11], many of which attribute this interaction to hydrophobic forces or electrostatics. ...
Trouble Shooting Guide
... but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it would do with the target sequence. Nevertheless, a binding and subsequent extension is poss ...
... but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it would do with the target sequence. Nevertheless, a binding and subsequent extension is poss ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
... at position 1 and still activated a Bicoid site target. LexABicoid mutant A5 contained a Lys+Ala substitution at position 5 and also activated a Bicoid site target but at reduced levels. However, activation by this mutant was also reduced using a LexA site target, perhaps because the protein is less ...
... at position 1 and still activated a Bicoid site target. LexABicoid mutant A5 contained a Lys+Ala substitution at position 5 and also activated a Bicoid site target but at reduced levels. However, activation by this mutant was also reduced using a LexA site target, perhaps because the protein is less ...
Diagnoses of HIV Infection among Adults and
... •Most HIV tests look for these antibodies, some look for the virus itself •The window period: period after infection but before the test becomes positive •Deciding when to get tested therefore depends on when you may have been ...
... •Most HIV tests look for these antibodies, some look for the virus itself •The window period: period after infection but before the test becomes positive •Deciding when to get tested therefore depends on when you may have been ...
Mutation Detection by Single Strand Conformation Polymorphism
... detection of bands of double-stranded DNA in polyacrylamide gel illuminated with UV-light has been described previously (Chehab and Kan, 1989). However, the sensitivity of this method was not high enough for SSCP analysis. Therefore, coupling SSCP analysis with an automated DNA sequencer enables hig ...
... detection of bands of double-stranded DNA in polyacrylamide gel illuminated with UV-light has been described previously (Chehab and Kan, 1989). However, the sensitivity of this method was not high enough for SSCP analysis. Therefore, coupling SSCP analysis with an automated DNA sequencer enables hig ...