Methods for detection of point mutations
... the type of base exchange on sensitivity [21]. In comparison with DNA-SSCP, higher sensitivities, especially for analysis of fragments of .200 bp, have been reported for RNA-SSCP [13]. Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of ...
... the type of base exchange on sensitivity [21]. In comparison with DNA-SSCP, higher sensitivities, especially for analysis of fragments of .200 bp, have been reported for RNA-SSCP [13]. Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of ...
The many twists and turns of DNA: template, telomere, tool, and target
... and the binding modes of small-molecule ligands and drugs. Despite all the progress, it cannot be overlooked that the sequence space has not been sampled very well. A handful of sequences make up a relatively large percentage of the entries. The Dickerson–Drew Dodecamer (DDD) of sequence d(CGCGAATTC ...
... and the binding modes of small-molecule ligands and drugs. Despite all the progress, it cannot be overlooked that the sequence space has not been sampled very well. A handful of sequences make up a relatively large percentage of the entries. The Dickerson–Drew Dodecamer (DDD) of sequence d(CGCGAATTC ...
Intrinsic sequence specificity of the Cas1 integrase directs
... strongest disintegration activity (Figure 7A). In E. coli, the -2 position in the leader is a guanine. A ...
... strongest disintegration activity (Figure 7A). In E. coli, the -2 position in the leader is a guanine. A ...
Isolation of insertion elements from Gram
... refers to a close relationship of these bacteria, or indicates that gene transfer events must have happened. IS-Cg and IS-B1 probably have a common ancestor, since both are similar in size, share homologous sequences and generate target site duplications of 8 bp (Fig. lb). In this study we extended ...
... refers to a close relationship of these bacteria, or indicates that gene transfer events must have happened. IS-Cg and IS-B1 probably have a common ancestor, since both are similar in size, share homologous sequences and generate target site duplications of 8 bp (Fig. lb). In this study we extended ...
CapeTownGenomes
... New sequencing technologies provide better representation of the genome (by removing cloning steps) and deeper coverage but are harder to assemble because of the short-read lengths. People now talk about the ‘accessible’ genome for a species. This simply means the output from a reasonably deep s ...
... New sequencing technologies provide better representation of the genome (by removing cloning steps) and deeper coverage but are harder to assemble because of the short-read lengths. People now talk about the ‘accessible’ genome for a species. This simply means the output from a reasonably deep s ...
Genetic dissection of Helicobacter pylori AddAB role in homologous
... single addB mutant is extremely sensitive to IR. Inactivating both genes, addA and addB, resulted in the same sensitivity as that of the single mutants (Fig. 2b). These results confirm that AddA and AddB act together in the repair of IR-induced DNA damage. Inactivation of the AddAB complex made the ...
... single addB mutant is extremely sensitive to IR. Inactivating both genes, addA and addB, resulted in the same sensitivity as that of the single mutants (Fig. 2b). These results confirm that AddA and AddB act together in the repair of IR-induced DNA damage. Inactivation of the AddAB complex made the ...
Mapping strategies for sequence reads (with focus on RNA-seq)
... uires a set of known junctions from the reference ond, the QPALMA pipeline’s initial mapping phase ...
... uires a set of known junctions from the reference ond, the QPALMA pipeline’s initial mapping phase ...
A unique pattern of intrastrand anomalies in base
... become unscrambled and ligated in the orthodox order in conjunction with IES removal from polytene chromosomes. The polytene chromosomes are then fragmented, and ∼95% or more of the original micronuclear DNA sequence complexity is eliminated. These eliminated sequences are the spacers in micronuclea ...
... become unscrambled and ligated in the orthodox order in conjunction with IES removal from polytene chromosomes. The polytene chromosomes are then fragmented, and ∼95% or more of the original micronuclear DNA sequence complexity is eliminated. These eliminated sequences are the spacers in micronuclea ...
Bacterial Systems for Testing Spontaneous and
... (Janion, 2008). One of the symptoms of the SOS-induction is filamentous growth of bacteria due to expression of the sulA gene. The sulA gene codes for a protein that blocks cell division by inhibiting assembling of the FtsZ protein into a ring structure leading to filament formation (Bi & Lutkenhaus ...
... (Janion, 2008). One of the symptoms of the SOS-induction is filamentous growth of bacteria due to expression of the sulA gene. The sulA gene codes for a protein that blocks cell division by inhibiting assembling of the FtsZ protein into a ring structure leading to filament formation (Bi & Lutkenhaus ...
E.coli
... Purpose: When the single-stranded DNA of a fragment is required, a M 13 vector can be used as a common cloning tool. Preparation of ssDNA: 1. Cloning: standard plasmid cloning method can be used to incorporate recombinant DNA into M13 vectors; 2. Transformation: the M13 then infects sensitive E. col ...
... Purpose: When the single-stranded DNA of a fragment is required, a M 13 vector can be used as a common cloning tool. Preparation of ssDNA: 1. Cloning: standard plasmid cloning method can be used to incorporate recombinant DNA into M13 vectors; 2. Transformation: the M13 then infects sensitive E. col ...
Treble clef finger—a functionally diverse zinc
... Figure 1. Structural comparisons of representative treble clef fingers. (A) Structural diagrams of ribosomal protein S14 (1fjf, chain N, residues 21–53), ribosomal protein L24E (1ffk, chain R, residues 3–45), RING finger of RAG1 (dimerization domain) (1rmd, residues 23–60), ARF-GAP domain of Pyk2-as ...
... Figure 1. Structural comparisons of representative treble clef fingers. (A) Structural diagrams of ribosomal protein S14 (1fjf, chain N, residues 21–53), ribosomal protein L24E (1ffk, chain R, residues 3–45), RING finger of RAG1 (dimerization domain) (1rmd, residues 23–60), ARF-GAP domain of Pyk2-as ...
The Strengths, Weaknesses, Opportunities and Threats (SWOT
... poses significant challenges for diagnosis and treatment of new HIV infections. Optimal drug treatment requires ongoing, precise measurement of viral levels. If variant subtypes are present and significantly underquantified or undetected, therapy could be compromised. The genetic variability of the ...
... poses significant challenges for diagnosis and treatment of new HIV infections. Optimal drug treatment requires ongoing, precise measurement of viral levels. If variant subtypes are present and significantly underquantified or undetected, therapy could be compromised. The genetic variability of the ...
Chpt9_Transposition.doc
... instance, some retroviruses can integrate into a host genome to form endogenous retroviruses. Indeed, some viruses may be derived from natural transposable elements and vice versa. Since viruses move between individuals, at least some transposable elements can move between genomes (between individua ...
... instance, some retroviruses can integrate into a host genome to form endogenous retroviruses. Indeed, some viruses may be derived from natural transposable elements and vice versa. Since viruses move between individuals, at least some transposable elements can move between genomes (between individua ...
CB3 - Homework
... In a car factory, the central office has a number of computers in it. In the computers there are plans that contain information about how a car is to be built and the order in which the different pieces should be added. Information in these plans is stored in computer code. Instructions from the com ...
... In a car factory, the central office has a number of computers in it. In the computers there are plans that contain information about how a car is to be built and the order in which the different pieces should be added. Information in these plans is stored in computer code. Instructions from the com ...
Mendelian Terminology
... Mendel was able to establish three things based upon the evidence gathered from the pea plants. ...
... Mendel was able to establish three things based upon the evidence gathered from the pea plants. ...
The msh2 Gene of Schizosaccharomyces pombe Is
... In Escherichia coli, the mutHLS system efficiently repairs single-base mismatches except C/C, as well as small singlestrand insertions and deletions (45). In addition, this system maintains genome stability by prevention of recombination between homeologous sequences (55). The MutS protein recognize ...
... In Escherichia coli, the mutHLS system efficiently repairs single-base mismatches except C/C, as well as small singlestrand insertions and deletions (45). In addition, this system maintains genome stability by prevention of recombination between homeologous sequences (55). The MutS protein recognize ...
repair
... linkage is removed by substitution for a Cys, and the protein fails to fold properly, then function may be completely eliminated or changed. On the other hand, the substitution could occur in a completely innocuous manner and not change function at all, or the effect could be intermediate. Similarly ...
... linkage is removed by substitution for a Cys, and the protein fails to fold properly, then function may be completely eliminated or changed. On the other hand, the substitution could occur in a completely innocuous manner and not change function at all, or the effect could be intermediate. Similarly ...
Extracting Haplotypes from Diploid Organisms
... first using gene-specific PCR primers to amplify both alleles in diploid individuals. The PCR products are then sequenced directly using automated DNA sequencers, yielding a composite chromatogram for each locus in each individual organism. The composite DNA sequence data for each locus from many in ...
... first using gene-specific PCR primers to amplify both alleles in diploid individuals. The PCR products are then sequenced directly using automated DNA sequencers, yielding a composite chromatogram for each locus in each individual organism. The composite DNA sequence data for each locus from many in ...
DNA crosslinking and biological activity of a
... of DNA, and especially interstrand crosslinks, lead to cell death if not repaired. Because of their relatively simple structure and high reactivity, nitrogen mustards have a very limited DNA sequence selectivity and rapidly lose their activity by non-speci®c interaction with a number of nucleophilic ...
... of DNA, and especially interstrand crosslinks, lead to cell death if not repaired. Because of their relatively simple structure and high reactivity, nitrogen mustards have a very limited DNA sequence selectivity and rapidly lose their activity by non-speci®c interaction with a number of nucleophilic ...
マニュアル Megaprime DNA Labelling System
... The Megaprime systems allow DNA from a variety of sources to be labelled in vitro to high specific activity with 32P and other radionuclides. The specific activity of the probes generated by these systems will vary according to the specific activity of the labelled dNTP used. The standard Megaprime ...
... The Megaprime systems allow DNA from a variety of sources to be labelled in vitro to high specific activity with 32P and other radionuclides. The specific activity of the probes generated by these systems will vary according to the specific activity of the labelled dNTP used. The standard Megaprime ...
Interfering RNA
... nucleic acid-based methods or other means and the gene sequence was known. • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi se ...
... nucleic acid-based methods or other means and the gene sequence was known. • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi se ...
HLA-B27 real-time PCR using TaqMan
... recognise only B*27 specific sequences. The PCR reaction amplifies this region to sufficient quantity so that it may be visualised after electrophoresis in agarose gel. Visualisation is achieved by staining with ethidium bromide (a fluorescent DNA intercalating dye) and viewing the gel under UV ligh ...
... recognise only B*27 specific sequences. The PCR reaction amplifies this region to sufficient quantity so that it may be visualised after electrophoresis in agarose gel. Visualisation is achieved by staining with ethidium bromide (a fluorescent DNA intercalating dye) and viewing the gel under UV ligh ...
Dycal Radiopaque Calcium Hydroxide - Catalyst
... Not normally a hazard due to non-volatile nature of product The material can produce chemical burns within the oral cavity and gastrointestinal tract following ingestion. Accidental ingestion of the material may be damaging to the health of the individual. The material can produce chemical burns fol ...
... Not normally a hazard due to non-volatile nature of product The material can produce chemical burns within the oral cavity and gastrointestinal tract following ingestion. Accidental ingestion of the material may be damaging to the health of the individual. The material can produce chemical burns fol ...
pDsRed-Monomer-C1 Vector Information
... DsRed-Monomer coding sequence and the SV40 polyadenylation signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of DsRed-Monomer if they are in the same reading frame as DsRed-Monomer and there are no intervening stop codons. A Kozak consensus translation in ...
... DsRed-Monomer coding sequence and the SV40 polyadenylation signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of DsRed-Monomer if they are in the same reading frame as DsRed-Monomer and there are no intervening stop codons. A Kozak consensus translation in ...