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Genetic Engineering
Genetic Engineering

... Scientists also have employed recombinant DNA techniques to produce medically useful human proteins in animal milk. In this procedure, the human gene responsible for the desired protein is first linked to specific genes of the animal that are active only in its mammary (milkproducing) glands. The eg ...
BIOL290
BIOL290

... I. Understand how methylation of DNA can generate mutational “hot spots” J. Understand how Xeroderma pigmentosum and Cockayne Syndrome are the result of mutations in the same repair pathway. K. Know the different types of mutations associated with cancer, such as oncogene mutations and mutations in ...
Pathogen Genomics COURSE
Pathogen Genomics COURSE

... 3.3) The two major outliers appear to suggest that “membrane” proteins and “adhesins” may be important for pathogenesis of E. coli O157:H7. You can use the “Query” function in TaxPlot to highlight other membrane proteins and adhesins in the plot. Q6: Are there other membrane proteins and adhesins t ...
From DNA to Protein Name: What does DNA stand for? What is DNA
From DNA to Protein Name: What does DNA stand for? What is DNA

... 13. When replication is complete, how do the two new DNA molecules compare to each other and the original DNA molecule? ...
DNA replication - Seattle Central College
DNA replication - Seattle Central College

... • J. Watson & F. Crick deduced doublestranded, helical structure from Rosalind Franklin’s X-ray crystallographic image of a DNA molecule. ...
and Post-assessment multiple choice questions
and Post-assessment multiple choice questions

... B. All cells in a single colony are resistant to the antibiotic ampicillin. C. Only the founder cell of a single colony is resistant to the antibiotic ampicillin. D. Cells that did not take up the plasmid will survive on the medium. E. Each colony began with one antibiotic resistant cell and all cel ...
Section 8.7 Mutations
Section 8.7 Mutations

... Two Categories of Mutations: 1.Single Gene – affects one gene – usually caused by an error in DNA replication 2. Chromosomal – affects chromosomes – usually error in meiosis . Usually more harmful since many genes are affected. ...
013368718X_CH15_229-246.indd
013368718X_CH15_229-246.indd

... fragments of DNA together? A. an amino acid sequence B. DNA ligase C. restriction enzymes D. mRNA 10. Why must a genetically engineered plasmid contain a genetic marker? A. to prevent the construction of an artificial chromosome B. to separate cells that contain recombinant DNA from those that do no ...
PowerPoint 簡報
PowerPoint 簡報

... • Concerted evolution and is one of the most important acting on multigene families because it means that mutations can spread to all members, even if they reside on different chromosomes. • It becomes difficult to discern which genes are really homologous, so that orthologous and paralogous gene ca ...
AP Biology (An Introduction)
AP Biology (An Introduction)

... 3. Combine the 2 DNA pieces (into a recombinant plasmid?)  Recombinant plasmid – plasmid + DNA fragments  Sealed together using DNA Ligase  Remember: we used ________ ________ to cut gene of ...
Exam #3 Study Guide
Exam #3 Study Guide

... STUDY GUIDE EXAM #3 ...
Chapter 11 A - Iowa State University
Chapter 11 A - Iowa State University

... iv. Variation- Differences in genetic materials must account for variations within a species. II. _________ are the building blocks of DNA and RNA. (if you don’t know this I dis-own you as my SI students : ) nucleotides III. What are the 3 components of the answer above? Phosphate, pentose sugar, ni ...
Slide 3 - Pulse 2012
Slide 3 - Pulse 2012

... structures seen in the human genome. Some (very few) genes do not have introns. One example is the histone genes, which encode the small DNA-binding proteins, histones H1, H2A, H2B, H3, and H4. Shown here is a histone gene that is only 400 base pairs (bp) in length and is composed of only one exon. ...
Document
Document

... Not uncommon to assemble trillions of sequence reads. Some things to consider: If error rates are high (454, Illumina) 30-50x genome sequencing is required. If error rates are low (SOLiD, Ion Torrent) 4-5x coverage is ...
4.2 Mutation - WordPress.com
4.2 Mutation - WordPress.com

... one of its chromosomes when it was a fertilized egg. Just one base changes in 1 out of 10 bears. The mutated gene will not show it colour white unless a bear gets one mutated gene from each parent (it needs two mutated genes to show the ...
Evelyn Section A
Evelyn Section A

... G+C are possible combination and A+T is not the same as T+A as well as C+G is not the same as G+C (1).The DNA contains the hereditary information that is innate by the brood of an organism; ‘this information is determined by the sequence of the base pair along its length"(1). ...
lizcar~1
lizcar~1

... generation of successful transgenics ...
E. coli
E. coli

... sequence of the genome of an organism, and the identification of all its genes • Genome projects are possible because of the large-scale, automated application of molecular genetic techniques (cloning and sequencing) • There are now complete genome projects for all major groups of organism ...
DNA- Replication - Seabreeze High School
DNA- Replication - Seabreeze High School

... DNA polymerase (an enzyme) brings bases to make “new” strands ...
DNA
DNA

... Bryophyta) contains 121,024 base pairs in a closed circle. These make up some 128 genes which include: •duplicate genes encoding each of the four subunits (23S, 16S, 4.5S, and 5S) of the ribosomal RNA (rRNA) used by the chloroplast •37 genes encoding all the transfer RNA (tRNA) molecules used for tr ...
DNA Typing
DNA Typing

... non repeating sequences, therefore lots of alleles are generally present in a population. In other words, two individuals have a higher chance of genetic differences at STR’s and VNTR’s than at most sequences in the DNA. ...
CHEM 331 Problem Set #7- Lehninger 5e, Chapter 8 Due Friday
CHEM 331 Problem Set #7- Lehninger 5e, Chapter 8 Due Friday

... 1,000 years. B. subtilis spores are much more resistant than are the organism’s growing cells to heat, UV radiation, and oxidizing agents, all of which promote mutations. a. One factor that prevents potential DNA damage in spores is their greatly decreased water content. How would this affect some t ...
stranded DNA from genomic library
stranded DNA from genomic library

... – Bacteria with gene of interest will glow ...
Pierce chapter 10
Pierce chapter 10

... • Series of 4 or more A-T base pairs cause DNA to bend – Affects ability of proteins to bind to DNA’ affects transcription ...
Mutations
Mutations

... • Transposons are useful for making mutants – Insertion is random, so there is the possibility of mutating the gene you are looking for. – Antibiotic resistance provides a “selectable marker” ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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