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Molecular Basis of Inheritance
Molecular Basis of Inheritance

...  Each strand of original DNA serves as a template  Nucleotides match to template according to base pairing rules  1 ‘parent’ DNA strand produces 2 new ‘daughter’ strands ...
Walsh
Walsh

... Metallothionein: Nature’s back-up system, Selenium: Speeds up the process. ...
BACTERIAL GENETICS CH. 6,7,8
BACTERIAL GENETICS CH. 6,7,8

... 1). Produced complementary to 1/2 DNA (master strand, active side) 2). Equal to one gene (code for one protein) 3). Three nucleotides = codon 4). Codon = code for one specific amino acid 5). Long, linear, single helix b. Function 1). Carries code for production of one protein 2). Site of protein syn ...
Bio 102 Practice Problems
Bio 102 Practice Problems

... 1. Recombinant human insulin, produced by bacteria carrying a cloned insulin gene, is now the major form of insulin used to treat diabetes. The human insulin gene encodes an mRNA only 333 nucleotides long, but the entire gene spans more than 4000 nucleotides. There are three exons and two introns. a ...
Electrical induction hypothesis to explain enhancer-promoter
Electrical induction hypothesis to explain enhancer-promoter

... been understood about three‐dimensional chromosome conformation beyond the scale of the nucleosome. But, recent advances in molecular biology and computational analysis have lent insight into chromatin interactions on a larger scale. Chromosome conformation capture (3C) methodology was developed to ...
Bioinformatic Analysis: Designing primers and annotation gene of
Bioinformatic Analysis: Designing primers and annotation gene of

...  Copy the primer sequences into your online journal or your text file.  Name the primers with the gene name and append F or R o Example: the forward primer for the rbcL gene should be named rbcL-F o Enter the primer sequences into the Primer Order Form Annotate the Aiptasia or Symbiodinium gene (b ...
dna review - NVHSIntroBioPiper1
dna review - NVHSIntroBioPiper1

... Recombinant DNA – A gene is removed from a human chromosome and inserted into bacterial DNA. This programs the DNA in the bacteria to produce the chemicals that the gene is for (ex. insulin). This creates safer and more cost effective treatment for many diseases (ex. diabetes). ...
DNA Worksheet
DNA Worksheet

... Now, click the “Go on” green button again. Watch the animation closely. You will see how the four bases (A,T,C &G) can attach to the right side of the deoxyribose molecule. 21. As you move down the strand, any one of the four bases ___, ___, ___ or ___ will join onto the right side of each deoxyribo ...
Standard Biology Ch. 28 DNA
Standard Biology Ch. 28 DNA

... phosphate fill in on open middle section following base pairing rules (A=T and C=G) 2 new DNA are formed that are exactly alike ...
Name Date ______ Lab genetic engineering using bacteria In this
Name Date ______ Lab genetic engineering using bacteria In this

... 4. Cut the green human insulin gene as if you have used the a restriction enzyme, HindIII. Be sure to leave “sticky ends.” 5. Also, cut the white bacterial plasmid DNA with the restriction enzyme HindIII. Be sure to leave “sticky ends.” 6. Now you will incorporate the human insulin gene into the pl ...
DNA and Protein Synthesis Review Sheet
DNA and Protein Synthesis Review Sheet

... 24. What nitrogen base is present in DNA that is not in RNA? Thymine 25. Where in the cell is DNA located? Nucleus 26. Who discovered that the shape of DNA was a double helix? Watson and Crick 27. Be able to decode a codon to an amino acid. There are several types of mutation: DELETION (a base is lo ...
Document
Document

... of genes DNA segments from known genes (up to hundreds of bp long) are amplified by PCR and placed on a solid surface using robotic devices that accurately deposit nanoliter amounts of DNA solution Thousands of such spots are deposited in a pre-designed array on a surface area of just a ...
Zoo/Bot 3333
Zoo/Bot 3333

... the open slots representing the origin. 3. Which of the above lanes (left to right) on the electrophoresis gel represents a sequencing reaction containing deoxyadenosine triphosphate? a) the “G” lane; b) the “A” lane; c) the “T” lane; d) the “C” lane, e) all of the above. 4. The DNA sequence of the ...
DNA Structure
DNA Structure

... • There would be 20% Cytosine. Adenine (30%) = Thymine (30%) Guanine (20%) = Cytosine (20%) ...
DNA Notes
DNA Notes

... - When a cell is not dividing, DNA is in the form of chromatin. - Loosely packed DNA that is wrapped around proteins (called histones) ...
Recombinant DNA Technology
Recombinant DNA Technology

... segment of DNA in a form that can be duplicated in another cell • Requires a – Vector = carrier of DNA segment – Host = cell in which foreign DNA can be duplicated and its gene product may be synthesized ...
Ch08
Ch08

... G-C base pairs. Those “Watson-Crick” H-bonds that are part of holding the two DNA strands together which along with hydrophobic stacking allows DNA to form stable double stranded structure. But consider the protein that will bind to specific sequences in double stranded DNA…these proteins do not see ...
slides - The National Academies of Sciences, Engineering, and
slides - The National Academies of Sciences, Engineering, and

... Promise & Potential of Genome Editing in Somatic Cells ...
Biotechnology Notes
Biotechnology Notes

... • Recombinant DNA DNA that has been genetically modified by connecting DNA fragments from multiple sources • Host organism you are obtaining the gene from • Vector organism such as a bacteria, you are going to use to put the recombinant DNA into the organism you are trying to change • Plasmid DN ...
DNA Technology - wvhs.wlwv.k12.or.us
DNA Technology - wvhs.wlwv.k12.or.us

... **Junk DNA (introns) will be cut at different places for different people, therefore producing different size fragments ...
The human genome
The human genome

... occur every 100 to 300 bases along the 3-billion-base human genome. ...
Name______________________________________________
Name______________________________________________

... forms a layer on top of the soapy liquid. Add alcohol until you have about 2 cm of alcohol in the tube. Alcohol is less dense than water, so it floats on top. Do not mix or bump the test tube for 10 minutes. DNA molecules will clump together where the soapy water below meets the cold alcohol above, ...
Presentation - Anil Jegga - Cincinnati Children`s Hospital Medical
Presentation - Anil Jegga - Cincinnati Children`s Hospital Medical

... Change the “group” to “Custom Tracks” and select the appropriate “track” and “table” ...
Chapter 16 Recombination DNA and Genetic Engineering
Chapter 16 Recombination DNA and Genetic Engineering

... – Gene therapy promises a way to genetically alter the cells of the liver to keep the levels of cholesterol in the more normal range ...
Exam 3
Exam 3

... b. (2 Points) Describe a method that could help you purify a large amount of the mutant protein for biochemical studies. Answer: One could clone the gene downstream of the T7 promoter to express large amount of the protein or one could construct a variant that contains a His-tag on the N or C-termin ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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