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What is a mutation?
What is a mutation?

... • Missense : ANY mutation that changes the codon and makes a different amino acid in the protein • Nonsense : ANY mutation that changes a codon into one of the STOP codons • Silent : ANY mutation that causes no change in the protein and cannot be detected without sequencing the gene ...
DNA Structure, Replication and Genetic Code 25 points
DNA Structure, Replication and Genetic Code 25 points

... 16. Suppose a piece of DNA lost a base. How would it know what base to replace it with? It would use base pairing rules, and its opposing base, to tell it what base would be needed. 17. Is it possible for a cell to have different DNA than other cells in the same organism? Explain your answer. NO! Ev ...
Genetic Profiling using Short Tandem Repeat Analysis
Genetic Profiling using Short Tandem Repeat Analysis

... between  homologous  chromosomes,  while  the  flanking  regions  where  the  primers bind remain constant.  Allele A contains a tetranucleotide STR having 12  repeats (48 base pairs), and allele B has an STR with 8 repeats (32 base pairs).  Inheritance  of  STRs follows  basic  Mendelian  patterns. ...
DNA - jacybiology
DNA - jacybiology

... of their amplified DNA was probed, using a set of recombinant phage and cosmids containing a total of 380 kb of amplified DNA. In all 33 cell lines, the selected CAD gene and at least 65 kb of flanking DNA were amplified, an average of 2.6-fold. Six other regions of DNA were co-amplified in all 33 m ...
Chapter 15 Lecture Notes: Applications of Recombinant DNA
Chapter 15 Lecture Notes: Applications of Recombinant DNA

... (2) Toxic compounds from genetic manipulation (3) Unnatural method of food production c) Depending upon the source you get your info from, the FlavrSavr tomato was/was not a financial success. 3. Other examples of transgenic plants a) Herbicide resistant plants b) Cotton plants that are resistant to ...
Document
Document

... cells in a mature tobacco leaf is at least 10 times higher than the average number of leaf cells required to select one chloroplast gene transfer event, which indicates that cells within a single leaf are not genetically identical but may differ in their nuclear genome with respect to the pattern of ...
Chapter 12: Genetic Engineering
Chapter 12: Genetic Engineering

... Genetic engineering could not have come about without the development of a ______________________________ to support the process o A way to carefully _________________________ containing the gene away from the genes surrounding it o Find a way to ________________________________ with a piece of DNA ...
Discussion of control of the lac operon and mutational analysis
Discussion of control of the lac operon and mutational analysis

... I s=inducer binding region mutated Inhibits transcription, even with inducer ...
Mutation and Genetic Variation - Cal State LA
Mutation and Genetic Variation - Cal State LA

... (2) “mutation” hypothesis: in some tubes, a random mutation will happen early on & get passed to most offspring, prior to virus exposure - will give rise to occasional “jackpot cultures” that luckily got the resistance mutation early in their family tree - prediction: there will be wildly different ...
Tracing the Origins of a MRSA Epidemic (Article for Students)
Tracing the Origins of a MRSA Epidemic (Article for Students)

... USA300, in which the original speG gene is either replaced by a non-functioning mutant copy of the gene, or the gene is deleted from the genome altogether. They expected that the modified version of USA300 would not be able to alter or neutralize spermidine. First they had to prepare the knockout US ...
Transposition and transposable elements
Transposition and transposable elements

... • cut themselves out of original site, producing double strand break • cut target site and ligate to element ends, thereby inserting at new site • original site break repaired usually with sister chromosome, restoring transposon at original site sometimes end healed without transposon, can also be a ...
DNA Review Packet
DNA Review Packet

... S Then enzymes use each strand as a template S To assemble new nucleotides into complementary strands ...
downloadable  file
downloadable file

... Sequencing DNA is a way to determine the order of the four nucleotides along a strand of DNA. Sequencing DNA has become vital to the fields of basic research, biotechnology, forensics and medical diagnostics. In the late 1970’s, biology saw the first two methods to sequence DNA. One method, Maxam-Gi ...
Document
Document

... C. Missense, because a different amino acid is encoded by GTT compared to GAT. D. Frameshift, because an extra base is inserted into the sequence. C10. Here are two possible examples: The consensus sequences for many bacterial promoters are –35: 5–TTGACA–3 and –10: 5–TATAAT–3. Most mutations tha ...
C1. A. G→A, which is a transition. B. T→G, which is a transversion. C
C1. A. G→A, which is a transition. B. T→G, which is a transversion. C

... C. Missense, because a different amino acid is encoded by GTT compared to GAT. D. Frameshift, because an extra base is inserted into the sequence. C10. Here are two possible examples: The consensus sequences for many bacterial promoters are –35: 5′–TTGACA–3′ and –10: 5′–TATAAT–3′. Most mutations tha ...
Chapter 13 Mutation, DNA Repair, and Recombination
Chapter 13 Mutation, DNA Repair, and Recombination

... Simple tandem repeats are repeated sequence of one to six nucleotide pairs. Trinucleotide repeats can increase in copy number and cause inherited diseases. ...
The Impact of the Human Genome Project on Clinical Diagnostics
The Impact of the Human Genome Project on Clinical Diagnostics

... The publicly funded project started around 1990 with a goal to produce a highly accurate sequence by 2005 Celera started in 1998 and within 2 years sequenced more DNA than the publicly funded consortium! ...
biotechnology: tools and applications
biotechnology: tools and applications

... • Cotton resistant to boll weevil • Soybeans resistant to herbicide (Roundup) • Corn resistant to European corn borer ...
Modeling Mutations Activity
Modeling Mutations Activity

... 9. How has the point mutation changed the polypeptide chain from the original polypeptide chain? ___________________________________________________________________ _______________________________________________________________________________________ 10. How does this show evidence that not all m ...
DNA, RNA and the Genetic Code Worksheet
DNA, RNA and the Genetic Code Worksheet

... number of nucleotide strands ...
Socrative Modern Genetics - Manhasset Public Schools
Socrative Modern Genetics - Manhasset Public Schools

... 33. Which statement best describes the process of crossing-over? A) It takes place between homologous chromosomes and results in new gene combinations. B) It takes place between nonhomologous chromosomes and results in an increased gene mutation rate. C) It takes place between homologous chromosomes ...
國立彰化師範大學100 學年度碩士班招生考試試題
國立彰化師範大學100 學年度碩士班招生考試試題

... (C) are translated to produce basic DNA binding proteins (D) A and C are correct 16. If you want to make a genomic library with DNA fragments averaging about 45 kb in length, which vector will be most appropriate to use? (A) plasmid. (B) lamda phage (C) cosmid (D) bacteria artificial chromosome (BAC ...
More on Genetics2013
More on Genetics2013

... Restriction enzymes are used to cut the DNA into fragments containing genes and repeats. Note that the repeat fragments from these two samples are of different lengths. ...
DNA Quantification: Comparison of UV
DNA Quantification: Comparison of UV

... Method cannot be used to provide an estimation of DNA purity ...
HDBuzz - Huntington`s disease research news.
HDBuzz - Huntington`s disease research news.

... study were people with other CAG repeat disorders, to confirm and strengthen the connection between age of symptom onset and genes involved in DNA repair. Within each DNA repair gene they studied, they focused on tiny changes to a single nucleic base (like an A instead of a G). These one-letter spel ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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