Introduction and review Lecture 1: Jan. 18, 2006
... Genotype- The genetic constitution of an organism. Phenotype- The visible appearance of an organism. Homologous chromosomes- in a diploid organism, the 2 copies of a chromosome inherited from the mother and the father. Locus- Location of a gene on a chromosome. Allelomorph (allele)- different versio ...
... Genotype- The genetic constitution of an organism. Phenotype- The visible appearance of an organism. Homologous chromosomes- in a diploid organism, the 2 copies of a chromosome inherited from the mother and the father. Locus- Location of a gene on a chromosome. Allelomorph (allele)- different versio ...
Repair mechanisms - Pennsylvania State University
... • Last resort for DNA repair, e.g when repair has not occurred prior to replication. How does the polymerase copy across a non-pairing, mutated base, or an apyrimidinic/apurinic site? – DNA polymerase III usually dissociates at a nick or a lesion. – But replication can occur past these lesions, espe ...
... • Last resort for DNA repair, e.g when repair has not occurred prior to replication. How does the polymerase copy across a non-pairing, mutated base, or an apyrimidinic/apurinic site? – DNA polymerase III usually dissociates at a nick or a lesion. – But replication can occur past these lesions, espe ...
Specialties: Microbial Diversity and Bacterial Membrane Proteins
... I am interested in anything related to bacteriology and that is why I have several seemingly distinct research projects going on in my lab. All these projects employ similar molecular techniques, including bacterial genomic DNA preparation, PCR amplification, gene cloning, bacterial transformation, ...
... I am interested in anything related to bacteriology and that is why I have several seemingly distinct research projects going on in my lab. All these projects employ similar molecular techniques, including bacterial genomic DNA preparation, PCR amplification, gene cloning, bacterial transformation, ...
Plasmid w/ kanamycin resistance (pKAN)
... • Obtain the plasmids (pKAN and pAMP) P stands for plasmid pKAN = plasmid with antibiotic kanamycin resistance pAMP = plasmid with antibiotic ampicillin resistance ...
... • Obtain the plasmids (pKAN and pAMP) P stands for plasmid pKAN = plasmid with antibiotic kanamycin resistance pAMP = plasmid with antibiotic ampicillin resistance ...
Obtain PCR-Ready Genomic DNA from Buccal Cells, HeLa Cells, Hair
... Swab and rotated 5 times in the QuickExtract Solution to disperse the cells. • 104 counted human cervical carcinoma tissue culture (HeLa) cells. ...
... Swab and rotated 5 times in the QuickExtract Solution to disperse the cells. • 104 counted human cervical carcinoma tissue culture (HeLa) cells. ...
Ch 13 student notes
... d. However breeders can produce useful mutations 4. The use of mutations is particularly useful with bacteria a. Their small size enables millions of organisms to be treated with radiation or chemicals at the same time b. Using this technique, scientists have been able to develop hundreds of benefic ...
... d. However breeders can produce useful mutations 4. The use of mutations is particularly useful with bacteria a. Their small size enables millions of organisms to be treated with radiation or chemicals at the same time b. Using this technique, scientists have been able to develop hundreds of benefic ...
Mutations - Kent City School District
... Point mutations are single nucleotide base changes in a gene's DNA sequence. This type of mutation can change the gene's protein product in the following ways: ...
... Point mutations are single nucleotide base changes in a gene's DNA sequence. This type of mutation can change the gene's protein product in the following ways: ...
word - marric.us
... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
DNA, RNA and Protein Synthesis
... DNA, RNA and Protein Synthesis c. Complementary Bases In 1949, Erwin Chargaff observed that the percentage of adenine equals the percentage of thymine and the percentage of cytosine and guanine are also equal to each other in the DNA of a variety of organisms. 1. This observation was key to under ...
... DNA, RNA and Protein Synthesis c. Complementary Bases In 1949, Erwin Chargaff observed that the percentage of adenine equals the percentage of thymine and the percentage of cytosine and guanine are also equal to each other in the DNA of a variety of organisms. 1. This observation was key to under ...
File
... The promoter allows efficient transcription of the inserted gene and the operator permits regulation. The ribosome-binding site provides sequence signals for the efficient translation of mRNA derived from the gene. The gene to be expressed must include a sequence specific to the host cell, whi ...
... The promoter allows efficient transcription of the inserted gene and the operator permits regulation. The ribosome-binding site provides sequence signals for the efficient translation of mRNA derived from the gene. The gene to be expressed must include a sequence specific to the host cell, whi ...
Lab22
... DNA Synthesis in a tube (PCR) 1. Double stranded DNA template must be separated 2. DNA primers base pair to ends of single stranded target sequence 3. DNA polymerase adds nucleotides to the 3’ end of the primers by complementary base pairing free nucleotides to the template strand 4. Repeat steps 1 ...
... DNA Synthesis in a tube (PCR) 1. Double stranded DNA template must be separated 2. DNA primers base pair to ends of single stranded target sequence 3. DNA polymerase adds nucleotides to the 3’ end of the primers by complementary base pairing free nucleotides to the template strand 4. Repeat steps 1 ...
Macromolecular Sequence Analysis Introduction
... overlapping sequences. The resulting data are contigs of different lengths as well as shorter unassembled fragments. The availability of completely sequenced "reference" genomes may assist in the assembly process for closely related genomes. In the absence of ...
... overlapping sequences. The resulting data are contigs of different lengths as well as shorter unassembled fragments. The availability of completely sequenced "reference" genomes may assist in the assembly process for closely related genomes. In the absence of ...
Gel Electrophoresis
... Gel matrix acts as a “seive” for DNA Large DNA molecules cannot pass through the small holes in the gel Small molecules move easily through the gel ...
... Gel matrix acts as a “seive” for DNA Large DNA molecules cannot pass through the small holes in the gel Small molecules move easily through the gel ...
UNIT 3 MOLECULAR GENETICS: REVIEW QUESTIONS Which
... Why? What advantages does this have for the cell? 15. Distinguish between the three related terms: a) silent mutation, missense mutation, nonsense mutation b) frameshift mutation, point mutation, translocation c) histones, nucleosomes, centromeres d) LINES, SINES, telomeres 16. How can a mutagenic a ...
... Why? What advantages does this have for the cell? 15. Distinguish between the three related terms: a) silent mutation, missense mutation, nonsense mutation b) frameshift mutation, point mutation, translocation c) histones, nucleosomes, centromeres d) LINES, SINES, telomeres 16. How can a mutagenic a ...
Mouse Genetics
... Advantages-always have a phenotype, can select for particular organ system/stage of development/tissue type; can get hypomorphic alleles (new alleles of existing mutations) Disadvantages-time consuming especially for recessive screens; works best with robust phenotypes;must go through a round of ...
... Advantages-always have a phenotype, can select for particular organ system/stage of development/tissue type; can get hypomorphic alleles (new alleles of existing mutations) Disadvantages-time consuming especially for recessive screens; works best with robust phenotypes;must go through a round of ...
Restriction Enzyme Digestion
... • Usually, organisms that make restriction enzymes also make a companion modification enzyme (DNA methyltransferasemethylase) that protects their own DNA from cleavage. • These enzymes recognize the same DNA sequence as the restriction enzyme they accompany, but instead of cleaving the sequence, the ...
... • Usually, organisms that make restriction enzymes also make a companion modification enzyme (DNA methyltransferasemethylase) that protects their own DNA from cleavage. • These enzymes recognize the same DNA sequence as the restriction enzyme they accompany, but instead of cleaving the sequence, the ...
DNA Structure and Function
... for how the cells of all living things construct proteins. B. DNA contains genes = specific locations on DNA ...
... for how the cells of all living things construct proteins. B. DNA contains genes = specific locations on DNA ...
DNA Replication - cloudfront.net
... Semiconservative Model of Replication • New DNA consists of 1 PARENTAL (template) and 1 NEW (complementary) strand of DNA DNA Template Parental DNA ...
... Semiconservative Model of Replication • New DNA consists of 1 PARENTAL (template) and 1 NEW (complementary) strand of DNA DNA Template Parental DNA ...
Nucleotide is composed of a ribose sugar, a base and a phosphate
... suitable media for isolation of strains. In bacteria, exchange of DNA from another cell. Consequences of recombination include new genotypes and phenotypes, eg. Ability to synthesis a new enzyme, antibiotic resistance. Strains carrying recombinant DNA are termed recombinants Homologous recombination ...
... suitable media for isolation of strains. In bacteria, exchange of DNA from another cell. Consequences of recombination include new genotypes and phenotypes, eg. Ability to synthesis a new enzyme, antibiotic resistance. Strains carrying recombinant DNA are termed recombinants Homologous recombination ...
Molecular Genetics - Ursuline High School
... will need to perform its life functions. As a result of Replication, the Mother cell copies its DNA and gives an exact copy to each resulting Daughter cell. This ensures that the Daughter cells will have the genetic information (DNA) they need to survive. ...
... will need to perform its life functions. As a result of Replication, the Mother cell copies its DNA and gives an exact copy to each resulting Daughter cell. This ensures that the Daughter cells will have the genetic information (DNA) they need to survive. ...
Introduction to Genetics
... will always exhibit that trait. Dominant expressed by capital letter ...
... will always exhibit that trait. Dominant expressed by capital letter ...
genetic engineering - Skinners` School Science
... DNA found in bacteria) containing foreign genes by treating them with calcium salts. The cells receiving the plasmids are transgenic. Transgenic organisms contain additional DNA which has come from another organism The transgenic bacteria can be cultured and will express the inserted genes as if the ...
... DNA found in bacteria) containing foreign genes by treating them with calcium salts. The cells receiving the plasmids are transgenic. Transgenic organisms contain additional DNA which has come from another organism The transgenic bacteria can be cultured and will express the inserted genes as if the ...
Chapter 16 – The Molecular Basis of Inheritance
... In the diagram below, label the following: leading and lagging strands, Okazaki fragments, DNA polymerase, DNA ligase, helicase, primase, single-stranded binding protein (SSBP), RNA primer, replication fork, 5’ end, 3’ end. ...
... In the diagram below, label the following: leading and lagging strands, Okazaki fragments, DNA polymerase, DNA ligase, helicase, primase, single-stranded binding protein (SSBP), RNA primer, replication fork, 5’ end, 3’ end. ...