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Topic 7 The Discovery of DNA & Its Roles
Topic 7 The Discovery of DNA & Its Roles

... Avery and colleagues spent 14 years testing various chemicals from the S bacterial remains to see which would transform nonpathogenic bacteria into pathogenic ones (R into S)  Only DNA worked ...
clicker review
clicker review

... D can process eukaryotic mRNA 13. In bacteria, a small self-replicating extrachromosomal ring of DNA is called a A prophage B RFLP C plasmid D cDNA E PCR 14. GFP is used as a genetic tool because it can A make many copies of a small amount of DNA B be used as a marker to tell which bacteria contain ...
Chapter 16 – The Molecular Basis of Inheritance
Chapter 16 – The Molecular Basis of Inheritance

... In the diagram below, label the following: leading and lagging strands, Okazaki fragments, DNA polymerase, DNA ligase, helicase, primase, single-stranded binding protein (SSBP), RNA primer, replication fork, 5’ end, 3’ end. ...
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... chromosome • Cloned genes are useful for making copies of a particular gene and producing a protein product ...
Zinc Carbonate - Di-Corp
Zinc Carbonate - Di-Corp

... use. Wear suitable protection for eyes and skin when handling. Use with adequate ventilation. Avoid contact with incompatible material. Store in cool, dry, well-ventilated area away from sources of heat, initiation and sparks. Eye wash and safety showers should be easily accessible and in proper ope ...
1 Mbp DNA for human genome
1 Mbp DNA for human genome

... Polymorphisms – occurrence of two or more variants (alleles, phenotypes, sequence variants) at significant frequencies in a population if present < 2% in population, called “mutation” or “mutant allele” Haplotype – set of alleles linked on a chromosome usually inherited together as a block, ...
The Production of a
The Production of a

... Transformation – the uptake and expression of foreign DNA by a cell Transduction – the use of viruses to transform or genetically engineer cells Endonucleases – enzymes that cut RNA or DNA at specific sites; restriction enzymes are endonucleases that cut DNA Sticky cells – restriction fragments in w ...
Use of DNA Technology:
Use of DNA Technology:

... • The number of repeats in each VNTR is unique amongst individuals (unless identical twins) • You share roughly 50% of these repeats in common with your parents and siblings ...
Whole genome assembly from next generation sequencing
Whole genome assembly from next generation sequencing

... sequencing, successfully traverse many of these repetitive elements, but are associated with higher costs, and even these improved sequencing methods often fall short of complete chromosome assembly. Alternative innovative strategies are overcoming the challenge of generating long contiguous genomic ...
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No Slide Title

... DMS + Piperidine DMS + Formic acid + Piperidine Hydrazine + Piperidine Hydrazine + 1.5 M NaCl + Piperidine ...
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No Slide Title

... mutations from an active transposon or from transgene insertion 2) Find a individual that has the expected phenotype of a mutant of YFG 3) Use the cloned transgene/transposon as a probe to clone the mutated gene out of a library of clones made from the mutant 4) Use the cloned mutant gene to isolate ...
Directed Reading A
Directed Reading A

... a. inherited characteristics c. cells and structures b. generations d. protein and DNA ______ 2. What is the name of the material that determines inherited characteristics? a. deoxyribonucleic acid c. RNA b. ribosome d. amino acid ...
GPVEC 2008 Biotech part 1
GPVEC 2008 Biotech part 1

... of disease-resistant wheat varieties by cross-breeding different wheat types until the desired disease resistance was present in a resulting new variety. ...
Recombinant DNA (DNA Cloning)
Recombinant DNA (DNA Cloning)

... • Cloning vector - carrier for moving DNA into a cell; such as a bacterial virus or plasmid into which foreign DNA can be inserted • Recombinant DNA: joining together of two fragments of DNA that are not normally joined together (e.g. joining together of eukaryotic DNA and prokaryotic DNA - usually ...
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... 1. Describe Fred Sanger's method of sequencing the order of DNA nucleotides in a gene. (Module 23) Include in your description the role of the following: dideoxynucleotides with radioactive phosphorus (chain terminators); polyacrylamide gels and how these gels reveal the order of nucleotides in a D ...
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... 6.) Start from the end of the taped down string, place a Sugar, then a Phosphate, alternating with the sugars and phosphates, and then tape the 12 Sugars and 12 Phosphates on each string. For the other string, start with a Phosphate and end with a sugar. 7.) To each Sugar on one strand, tape a nitro ...
Recombinant Biotechnology
Recombinant Biotechnology

... • Like other enzymes restriction enzymes show specificity for certain substrates, and will only digest DNA within specific sequences of bases - called recognition sequence or a restriction site. • Some restriction enzymes cut DNA into overhanging single stranded ends. • Others will generate fragmen ...
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No Slide Title

... construction ...
DNA and RNA
DNA and RNA

... Long, single strand of nucleotides. Nitrogen bases: A,U,G,C no Thymine! Sugar: Ribose Found in cytoplasm and nucleus Types: messenger, transfer, ribosomal Function: Involved in the synthesis of protein molecules. ...
Gonzalez, 2005
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... A defining feature of T-helper cells The REQUIRED receptor for HIV entry ...
DNA structure lab protocol
DNA structure lab protocol

... DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the information available for building and maintaining an organism, similar to the way in which letters of the alphabet appear in a certain ord ...
PowerPoint 簡報
PowerPoint 簡報

... – Treat proteins to denature subunits with detergent such as SDS • SDS coats polypeptides with negative charges so all move to anode • Masks natural charges of protein subunits so all move relative to mass not charge ...
DNA, RNA, and Protein Synthesis: Information to
DNA, RNA, and Protein Synthesis: Information to

... 1. What are the bases that make up DNA? a. G1, G2, G3, G4 b. thymine, cytosine, adenine, guanine c. uracil, cytosine, adenine, guanine d. thymine, glycine, lysine, alanine 2. Which best describes the shape of DNA? a. circular b. long straight chain c. three leaf clover d. double helix 3. The four fu ...
作业习题
作业习题

... with bound ligand inhibits transcription. ...
PowerLecture: Chapter 13
PowerLecture: Chapter 13

... DNA polymerase can read correct sequence from complementary strand and, together with DNA ligase, can repair mistakes in incorrect strand ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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