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lecture 7 (BY 14)
lecture 7 (BY 14)

... HOW CELLS DUPLICATE THEIR DNA  Before a cell divides, enzymes and other proteins copy its DNA ...
Lecture 1 - Portal UniMAP
Lecture 1 - Portal UniMAP

... Parkinson's disease - development of L-Dopa as a therapeutic agent while working in Toronto. • 1961 Discovery of the hematopoietic stem cell by Toronto researchers • 1975 George Kohler and Cesar Milstein show that fusing cells can generate monoclonal antibodies. • • 1982 First genetically engineered ...
L26_ABPG2014
L26_ABPG2014

... •Genes have the ability to recognise similarities in each other from a distance, without any proteins or other biological molecules aiding the process, according to new research. This discovery could explain how similar genes find each other and group together in order to perform key processes invol ...
Genes in Pieces (PowerPoint) Northeast 2012
Genes in Pieces (PowerPoint) Northeast 2012

... What will remain after treatment with nuclease? Sketch a diagram and discuss with your neighbor. ...
3_DNA coloring and questions
3_DNA coloring and questions

... can occur in any order along a strand of DNA. The order of these bases is the code the contains the instructions. For instance ATGCACATA would code for a different gene than AATTACGGA. A strand of DNA contains millions of bases. (For simplicity, the image only contains a few.) Note that the bases at ...
Sequencing a genome
Sequencing a genome

... to as having an X-fold coverage. Low coverage (2x) is sufficient for gene discovery and some regulatory element identification. High coverage (6x) is good for gene annotation. There will still be some missing genes. Finished sequence has no gaps and is presumed to contain all genes. ...
Genetic Test Review Packet What is a Punnet square and what is it
Genetic Test Review Packet What is a Punnet square and what is it

... Ww Ww There is a 0% probability that there will be a rat with black fur, 100% of them will have white fur. Define the following terms: 7. Allele- one of a pair of genes that determine a specific trait. 8. Phenotype- the physical appearance of an organism. 9. Genotype- the set of genes carried by an ...
Assignment 4: The mutation
Assignment 4: The mutation

... step should be? What question will the researchers ask? At this stage, the scientists must find the difference between the allele that is considered normal and the one that is considered to be a mutant. In other words, they must locate the mutation in the sequence of the allele that causes deafness ...
Molecular Techniques in Radiobiology Introduction The structure of
Molecular Techniques in Radiobiology Introduction The structure of

... Gene manipulation tools: hosts • Mammalian cells do not take up foreign DNA naturally; one of several tricks must be used to bypass natural barriers – Microinjection: DNA can be injected, cell by cell, directly into the nucleus through a fine glass needle. – Calcium phosphate precipitation: Cells ta ...
FIGURE 9.2
FIGURE 9.2

... A replication fork is formed by the opening of the origin of replication, and helicase separates the DNA strands. An RNA primer is synthesized, and is elongated by the DNA polymerase. On the leading strand, DNA is synthesized continuously, whereas on the lagging strand, DNA is synthesized in short s ...
Learning objectives
Learning objectives

... reaction (RT-PCR) can be used to determine how expression of a gene changes at different stages of embryonic development. 15. State two questions that could be addressed through genome-wide expression studies. 16. Explain how in vitro mutagenesis and RNA interference help researchers to discover the ...
Quiz-3
Quiz-3

... 28. Suppose the DNA fingerprints of the samples did not match with any suspects but they matched with the victim. What will be your guess about the case. 29. A comparative proteomic analysis of normal vs cancerous tissue indicated that there is a shift in the position of a protein spot in cancer tis ...
Learning objectives
Learning objectives

... reaction (RT-PCR) can be used to determine how expression of a gene changes at different stages of embryonic development. 15. State two questions that could be addressed through genome-wide expression studies. 16. Explain how in vitro mutagenesis and RNA interference help researchers to discover the ...
Extra Practice of Chargaff`s Rule and Complimentary Base Pairing
Extra Practice of Chargaff`s Rule and Complimentary Base Pairing

... You have decided you want to see what you have in common with other species as far as your DNA goes. You were able to sequence the DNA in E.coli, a mouse, and your big brother’s girlfriend (a human). Using Chargaff’s rule, discover which two organisms have the most DNA in common. Below is a cha ...
基因的性質和基因體 基因的性質和基因體
基因的性質和基因體 基因的性質和基因體

... 3. Run in opposite directions (antiparallel) 4. The backbone of each strand is on the outside of the molecule • 5. Chains held together by H bonds • 6. The sequence of one chain specifies the other's sequence (complementarity) ...
Document
Document

... • Gene therapy: the replacement of a faulty gene with a normal gene; the insertion of an extra gene with the intention that the gene product will play a therapeutic role. • Difficulty of gene therapy in practical terms. ...
fance - Baylor College of Medicine
fance - Baylor College of Medicine

... hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. Characteristic clinical features include developmental abnormalities in major organ systems, early-onset bone marrow failure, and a high predisposition to cancer. Definitive genotype/phenotype corr ...
Slide 1
Slide 1

... • How do we know DNA has the instructions to make proteins? • How does DNA instruct the cell to make proteins? ...
PDF
PDF

... directs a Cas9 nuclease to a specific genomic locus. Cas9 cleaves the gene of interest and creates a double­ stranded break (DSB) in the DNA, which can be repaired by non­homologous end joining (NHEJ). As NHEJ is an error­prone DNA repair process, insertions and deletions (indels) are often introduc ...
Biotechnology
Biotechnology

... today…including in your Genetics course (if you continue on in Biology) More advanced (and requiring much more expensive equipment) is the STR Profiling method = short tandem repeat profiling. STR does __ require use of restriction enzymes Newest method to produce DNA profiles or “fingerprints” acco ...
Carrots and Genomics
Carrots and Genomics

... • Easy for traits determined by a single gene: one locus on the genome ...
sample
sample

... adenine, 2) cytosine, 3) guanine, and 4) thymine. Since these terms are used so many times by scientists, they refer to them as A, C, G, and T, respectively. A and G are purines- each consists of two diffused rings; and C and T are pyrimidines- each consists of one ring. The nucleotides are linked t ...
Nucleic Acids - cloudfront.net
Nucleic Acids - cloudfront.net

... - Adenine pairs with Uracil ...
Week4 Lab Lecture
Week4 Lab Lecture

... into plasmids with compatible sticky ends • Ideally this creates a library of all of the restriction fragments from the digest • These can be transformed in to competent E. coli and which can then be screened for the plasmid containing the fragment of interest. (What is the phenotype of the lux oper ...
Biotechnology: Principles, Applications, and Social Implications
Biotechnology: Principles, Applications, and Social Implications

... An aim of therapeutic cloning is to provide stem cells for a patient, which requires a transplant Technique of embryo division – old technique of formation genetically identical individuals, division of morula or blastocyst ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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