Visualizing structural variations of single DNA molecules
... We used our device to image fluorescence barcodes generated on mega base pair long human DNA. Human genomic DNA was obtained by proteolysis of metaphase chromosomes directly in the inlet wells of the device (figure 1B). The barcode was obtained by staining the DNA homogeneously with YOYO-1 followed ...
... We used our device to image fluorescence barcodes generated on mega base pair long human DNA. Human genomic DNA was obtained by proteolysis of metaphase chromosomes directly in the inlet wells of the device (figure 1B). The barcode was obtained by staining the DNA homogeneously with YOYO-1 followed ...
Biology: DNA Review Packet
... 1. Label EVERY sugar (S), phosphate (P), and nitrogen base (A, T, C, G) in the diagram below. ...
... 1. Label EVERY sugar (S), phosphate (P), and nitrogen base (A, T, C, G) in the diagram below. ...
DNA Extraction, PCR Amplification and Sequencing: the IGS
... tissues preserved in CTAB buffer, were placed in 2.0 ml microcentrifuge tubes with 4-5 3 mm glass beads and macerated using a Mini-BeadBeater-8 (BioSpec Products Inc., Bartlesville, OK) set at 3/4 speed for one minute (100 ml of 2X CTAB is 10 ml of a 1 M stock of TRIS-HCL pH 8.0, 28 ml of a 5 M stoc ...
... tissues preserved in CTAB buffer, were placed in 2.0 ml microcentrifuge tubes with 4-5 3 mm glass beads and macerated using a Mini-BeadBeater-8 (BioSpec Products Inc., Bartlesville, OK) set at 3/4 speed for one minute (100 ml of 2X CTAB is 10 ml of a 1 M stock of TRIS-HCL pH 8.0, 28 ml of a 5 M stoc ...
8.3 DNA Replication
... Proteins carry out the process of replication. • DNA serves only as a template…enzymes and other proteins do the actual work of replication. 1. Enzymes unzip the double helix in two directions at the same time. 2. Free-floating nucleotides pair with the exposed bases on the template strand (and they ...
... Proteins carry out the process of replication. • DNA serves only as a template…enzymes and other proteins do the actual work of replication. 1. Enzymes unzip the double helix in two directions at the same time. 2. Free-floating nucleotides pair with the exposed bases on the template strand (and they ...
Next generation sequencing
... signature sequence library is equivalent to the genome. This library is cloned on beads using emulsion PCR technology. A water-in-oil emulsion is created from a PCR mix that contains a limiting dilution of DNA and beads. The emulsion creates micro-compartments with, on average, a single bead and sin ...
... signature sequence library is equivalent to the genome. This library is cloned on beads using emulsion PCR technology. A water-in-oil emulsion is created from a PCR mix that contains a limiting dilution of DNA and beads. The emulsion creates micro-compartments with, on average, a single bead and sin ...
dna technology and genomics
... 1) Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome. 2) Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3) Explain how the creation of sticky ends by restriction enzymes is useful i ...
... 1) Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome. 2) Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3) Explain how the creation of sticky ends by restriction enzymes is useful i ...
Document
... The two strands in the double helix run in opposite directions, with the nitrogenous bases in the center. Each strand carries a sequence of nucleotides, arranged almost like the letters in a four letter alphabet for recording genetic information. Hydrogen bonds hold the strands together. The bonds a ...
... The two strands in the double helix run in opposite directions, with the nitrogenous bases in the center. Each strand carries a sequence of nucleotides, arranged almost like the letters in a four letter alphabet for recording genetic information. Hydrogen bonds hold the strands together. The bonds a ...
Replication PP
... • Complementary Sequences: Two strands of nucleotides that stick together because they have “opposite” sequences that form “base pairs”. • (A-T; G-C) Hey, nice sequence! ...
... • Complementary Sequences: Two strands of nucleotides that stick together because they have “opposite” sequences that form “base pairs”. • (A-T; G-C) Hey, nice sequence! ...
DNA and Protein Synthesis Review Sheet
... DNA replication means that the entire strand of DNA is copied. You end up with two double stranded DNAs that are full length ...
... DNA replication means that the entire strand of DNA is copied. You end up with two double stranded DNAs that are full length ...
The Molecular Nature of Genes
... 19. Which of the following would prevent the formation of a phosphodiester bond in DNA? A. removal of a nitrogen from one of the bases B. removal of oxygen from carbon 2 of the sugar C. removal of an oxygen from carbon 3 of the sugar D. removal of the phosphate from the alpha position of the nucleos ...
... 19. Which of the following would prevent the formation of a phosphodiester bond in DNA? A. removal of a nitrogen from one of the bases B. removal of oxygen from carbon 2 of the sugar C. removal of an oxygen from carbon 3 of the sugar D. removal of the phosphate from the alpha position of the nucleos ...
BioSc 231 Exam 5 2005
... A. chromosomal DNA which has been isolated from a donor organism. B. complementary DNA that is generated by using reverse transcriptase to make DNA from mRNA. C. cloned DNA that has been introduced into a cloning vector. D. cut DNA that has been digested with a restriction endonuclease for use in a ...
... A. chromosomal DNA which has been isolated from a donor organism. B. complementary DNA that is generated by using reverse transcriptase to make DNA from mRNA. C. cloned DNA that has been introduced into a cloning vector. D. cut DNA that has been digested with a restriction endonuclease for use in a ...
Fast, high-resolution DNA sizing with the fragment analyzer system
... Fast, High-Resolution DNA Sizing with the Fragment Analyzer™ System Accurately size DNA up to 50 kb in 1 hour for large-insert SMRTbell™ libraries The Fragment Analyzer™ instrument is a fast, high-resolution benchtop capillary electrophoresis (CE) platform that utilizes proprietary markers to accura ...
... Fast, High-Resolution DNA Sizing with the Fragment Analyzer™ System Accurately size DNA up to 50 kb in 1 hour for large-insert SMRTbell™ libraries The Fragment Analyzer™ instrument is a fast, high-resolution benchtop capillary electrophoresis (CE) platform that utilizes proprietary markers to accura ...
Molecular Basis of Inheritance
... Each strand of original DNA serves as a template Nucleotides match to template according to base pairing rules 1 ‘parent’ DNA strand produces 2 new ‘daughter’ strands ...
... Each strand of original DNA serves as a template Nucleotides match to template according to base pairing rules 1 ‘parent’ DNA strand produces 2 new ‘daughter’ strands ...
A O R P T Y S
... • Nitrogen BASES= Instructions for Proteins • Nitrogen bases are read in units of 3 called codons • Each codon represents 1 amino acid ...
... • Nitrogen BASES= Instructions for Proteins • Nitrogen bases are read in units of 3 called codons • Each codon represents 1 amino acid ...
PCR - churchillcollegebiblio
... What do these terms mean to you? You have 5 min to discuss possible meanings and examples with your group! ...
... What do these terms mean to you? You have 5 min to discuss possible meanings and examples with your group! ...
Sanger Sequencing Simulation
... enzymes, and minor improvements were made in the polyacrylamide gel apparatus; however, the essential technique remained unchanged for about 15 years. ...
... enzymes, and minor improvements were made in the polyacrylamide gel apparatus; however, the essential technique remained unchanged for about 15 years. ...
Notes
... Section 12.2 Structure of DNA Section 12.3 DNA Replication Section 12.1 Identifying the Substance of Genes Section 12.2 Structure of DNA DNA is a molecular structure containing the info that a cell needs to carry out all of its functions. In a way, ___________________________________________________ ...
... Section 12.2 Structure of DNA Section 12.3 DNA Replication Section 12.1 Identifying the Substance of Genes Section 12.2 Structure of DNA DNA is a molecular structure containing the info that a cell needs to carry out all of its functions. In a way, ___________________________________________________ ...
Using DNA to ID Pathogens
... Samples of Sue’s blood, urine, and lymph are collected at the first infirmary visit and are sent off for diagnostic laboratory tests. As part of a pilot study, the college infirmary is working with the molecular biology department at the college to identify pathogens by their DNA sequences. The lab ...
... Samples of Sue’s blood, urine, and lymph are collected at the first infirmary visit and are sent off for diagnostic laboratory tests. As part of a pilot study, the college infirmary is working with the molecular biology department at the college to identify pathogens by their DNA sequences. The lab ...
DNA Notes
... • Was able to tell that DNA had an “X” like structure where the strands are twisted around each other • All of these discoveries led to the following…. ...
... • Was able to tell that DNA had an “X” like structure where the strands are twisted around each other • All of these discoveries led to the following…. ...
Manipulating DNA Notes
... to copy pieces of DNA • When a “colored” base of DNA is added, replication is stopped – The “colored” bases show up on the electrophoresis (like previous picture!) ...
... to copy pieces of DNA • When a “colored” base of DNA is added, replication is stopped – The “colored” bases show up on the electrophoresis (like previous picture!) ...
Introduction to molecular biology
... responsible of the color of the eyes in fruit flies would be located on the X chromosome. He therefore propose that the genetic information may be supported by the chromosomes. ...
... responsible of the color of the eyes in fruit flies would be located on the X chromosome. He therefore propose that the genetic information may be supported by the chromosomes. ...
What does DNA look like?
... where the bases meet. The bases on each side of the molecule are used as a pattern for a new strand. As the bases on the original molecule are exposed, complementary nucleotides are added to each side of the ladder. Two DNA molecules are formed. Half of each of the molecules is old DNA, and half is ...
... where the bases meet. The bases on each side of the molecule are used as a pattern for a new strand. As the bases on the original molecule are exposed, complementary nucleotides are added to each side of the ladder. Two DNA molecules are formed. Half of each of the molecules is old DNA, and half is ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.