DNA - Shippensburg University
... • Helicases are enzymes that untwist the double helix at the replication forks • Single-strand binding protein binds to and stabilizes single-stranded DNA until it can be used as a template • Topoisomerase corrects “overwinding” ahead of replication forks by breaking, swiveling, and rejoining DNA st ...
... • Helicases are enzymes that untwist the double helix at the replication forks • Single-strand binding protein binds to and stabilizes single-stranded DNA until it can be used as a template • Topoisomerase corrects “overwinding” ahead of replication forks by breaking, swiveling, and rejoining DNA st ...
Automating the Promega Wizard® SV 96 Plasmid DNA Purification
... Automation of this process can deliver significant increases in throughput as well as higher levels of precision and accuracy. We have developed an automated method that enables processing of multiple 96-well plates per day using the Promega Wizard SV 96 Plasmid Purification System on the Hamilton M ...
... Automation of this process can deliver significant increases in throughput as well as higher levels of precision and accuracy. We have developed an automated method that enables processing of multiple 96-well plates per day using the Promega Wizard SV 96 Plasmid Purification System on the Hamilton M ...
HiPer® Real-Time PCR Teaching Kit
... are repeated 25 – 40 times and performed in a specialized instrument called thermal cycler. The Real-time PCR or Quantitative PCR is a modified approach compared to standard PCR, where the amplified DNA is detected as the reaction progresses instead of detection at the end. Quantitative PCR is carri ...
... are repeated 25 – 40 times and performed in a specialized instrument called thermal cycler. The Real-time PCR or Quantitative PCR is a modified approach compared to standard PCR, where the amplified DNA is detected as the reaction progresses instead of detection at the end. Quantitative PCR is carri ...
File - Molecular Biology 2
... produce many identical copies for subsequent biochemical analysis. In step 2, the ...
... produce many identical copies for subsequent biochemical analysis. In step 2, the ...
Structure and Physiological significance of lipid
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
Recombinant DNA Technology
... compatible host cells. In order to be propagated, the recombinant DNA molecule (insert DNA joined to vector DNA) must be introduced into a compatible host cell where it can replicate. The direct uptake of foreign DNA by a host cell is called genetic transformation (or transformation). Recombinant DN ...
... compatible host cells. In order to be propagated, the recombinant DNA molecule (insert DNA joined to vector DNA) must be introduced into a compatible host cell where it can replicate. The direct uptake of foreign DNA by a host cell is called genetic transformation (or transformation). Recombinant DN ...
Identification of the target DNA sequence and characterization of
... (14). The homology model of the protein and the very recently solved crystal structure (Protein Data Bank (PDB) ID: 4OOI, (15)) show that the protein is a homodimer with a winged helix-turn-helix (wHTH) motif (Supplementary Figure S1). Unlike the other metallorepressor members of its family, HlyU Vc ...
... (14). The homology model of the protein and the very recently solved crystal structure (Protein Data Bank (PDB) ID: 4OOI, (15)) show that the protein is a homodimer with a winged helix-turn-helix (wHTH) motif (Supplementary Figure S1). Unlike the other metallorepressor members of its family, HlyU Vc ...
Electrophoresis, Blotting and Immunodetection Gel
... Extract DNA from agarose gels in a single 10min spin with this easy-to-use kit. A gel nebuliser converts agarose to a spray from which DNA is separated (via a 0.45µm Ultrafree-MC filter). Prepares gel-purified PCR products for sequencing or cloning without need for further purification. Device volum ...
... Extract DNA from agarose gels in a single 10min spin with this easy-to-use kit. A gel nebuliser converts agarose to a spray from which DNA is separated (via a 0.45µm Ultrafree-MC filter). Prepares gel-purified PCR products for sequencing or cloning without need for further purification. Device volum ...
1-2 Teacher
... increase the mutation rate by using radiation and chemicals. Breeders can often produce a few mutants with desirable characteristics that are not found in the original population. ...
... increase the mutation rate by using radiation and chemicals. Breeders can often produce a few mutants with desirable characteristics that are not found in the original population. ...
Polymerase Chain Reaction (PCR) - G
... piece of RNA to initiate DNA replication, which in a normal cell is synthesized by the RNA polymerase. In the PCR reaction, short complimentary double stranded oligos are added that bind the denatured DNA and act as origins of replications. These double stranded oligos are known as primers and ...
... piece of RNA to initiate DNA replication, which in a normal cell is synthesized by the RNA polymerase. In the PCR reaction, short complimentary double stranded oligos are added that bind the denatured DNA and act as origins of replications. These double stranded oligos are known as primers and ...
Polymerase Chain Reaction (PCR) - G
... piece of RNA to initiate DNA replication, which in a normal cell is synthesized by the RNA polymerase. In the PCR reaction, short complimentary double stranded oligos are added that bind the denatured DNA and act as origins of replications. These double stranded oligos are known as primers and ...
... piece of RNA to initiate DNA replication, which in a normal cell is synthesized by the RNA polymerase. In the PCR reaction, short complimentary double stranded oligos are added that bind the denatured DNA and act as origins of replications. These double stranded oligos are known as primers and ...
In vitro selection of restriction endonucleases by
... resistant to cleavage by restriction endonuclease HaeIII; thus, the biotin-label at the end of the DNA remained and could be used for affinity selection of uncleaved DNA encoding active methyltransferase with streptavidin beads (11). In this study, on the other hand, affinity selection of cleaved DN ...
... resistant to cleavage by restriction endonuclease HaeIII; thus, the biotin-label at the end of the DNA remained and could be used for affinity selection of uncleaved DNA encoding active methyltransferase with streptavidin beads (11). In this study, on the other hand, affinity selection of cleaved DN ...
Recovery of DNA for Forensic Analysis from Lip Cosmetics*
... are carried through the Chelex DNA extraction and amplification stages. This could be observed directly, as a number of the DNA extracts were seen to have a distinct pink or orange hue. However, while many of the extracts were pigmented, not all such extracts resulted in fluorescent artefacts occurr ...
... are carried through the Chelex DNA extraction and amplification stages. This could be observed directly, as a number of the DNA extracts were seen to have a distinct pink or orange hue. However, while many of the extracts were pigmented, not all such extracts resulted in fluorescent artefacts occurr ...
The danger signal plus DNA damage two- COPD Kazutetsu Aoshiba
... represents a transitional phase from innate immunity to acquired immunity; during this step, CD4+ T-cells and CD8+ T-cells proliferate and undergo differentiation and activation in response to dendritic cells presenting antigens released by necrotic cells and tissues. When immune tolerance during st ...
... represents a transitional phase from innate immunity to acquired immunity; during this step, CD4+ T-cells and CD8+ T-cells proliferate and undergo differentiation and activation in response to dendritic cells presenting antigens released by necrotic cells and tissues. When immune tolerance during st ...
Four-color DNA sequencing by synthesis using cleavable
... The completion of the Human Genome Project has set the stage for screening genetic mutations to identify disease genes on a genome-wide scale (1). Accurate high-throughput DNA sequencing methods are needed to explore the complete human genome sequence for applications in clinical medicine and health ...
... The completion of the Human Genome Project has set the stage for screening genetic mutations to identify disease genes on a genome-wide scale (1). Accurate high-throughput DNA sequencing methods are needed to explore the complete human genome sequence for applications in clinical medicine and health ...
A Tissue-Selective Mechanism
... More specifically, studies performed using mice modeling DM1, HD, spinocerebellar ataxia type 7 and spinocerebellar ataxia type 1 indicate that the chromatin, transcription and replication status at repeats modulates repeat instability through gene-specific cis-elements, which include CCCTC-binding ...
... More specifically, studies performed using mice modeling DM1, HD, spinocerebellar ataxia type 7 and spinocerebellar ataxia type 1 indicate that the chromatin, transcription and replication status at repeats modulates repeat instability through gene-specific cis-elements, which include CCCTC-binding ...
SPARK™ DNA Sample Prep Kit Illumina® Platform
... Let the beads air dry at room temperature with the lid off for 5 minutes on the magnet. While air drying the beads, determine the volume required to proceed with size selection (Z), where 100 µL ≤ Z ≥ 20 µL. Note: Due to the variety of size selection methods and volume requirements for each, we leav ...
... Let the beads air dry at room temperature with the lid off for 5 minutes on the magnet. While air drying the beads, determine the volume required to proceed with size selection (Z), where 100 µL ≤ Z ≥ 20 µL. Note: Due to the variety of size selection methods and volume requirements for each, we leav ...
XRCC1 interacts with the p58 subunit of DNA Pol a
... BRCT1 domain but not the BRCT2 domain (Figure 1C). To further characterize the interaction between XRCC1 and p58, we analyzed the localization of both endogenous proteins by immunofluorescence in asynchronous cells treated or not with HU. No or very few XRCC1 and p58 foci co-localized in untreated ce ...
... BRCT1 domain but not the BRCT2 domain (Figure 1C). To further characterize the interaction between XRCC1 and p58, we analyzed the localization of both endogenous proteins by immunofluorescence in asynchronous cells treated or not with HU. No or very few XRCC1 and p58 foci co-localized in untreated ce ...
Cybertory Manual (WP) - Attotron Biosensor Corporation
... Electrophoresis is a very common method for determining DNA fragment sizes. The phosphate groups of DNA make it an acid; they are highly negatively charged in aqueous solution at neutral pH. When negatively charged DNA molecules are placed in an electric field, they migrate toward the positive elect ...
... Electrophoresis is a very common method for determining DNA fragment sizes. The phosphate groups of DNA make it an acid; they are highly negatively charged in aqueous solution at neutral pH. When negatively charged DNA molecules are placed in an electric field, they migrate toward the positive elect ...
The Spectrum and Frequency of Self
... transposes by a cut-and-paste mechanism. It is the ability to frequently cut itself from the linear continuity of the chromosome by introducing double-strand breaks (DSBs) that makes Ac a powerful mutagen. The subsequent repair of these DSBs by the host’s enzymatic machinery rarely leaves the DNA in ...
... transposes by a cut-and-paste mechanism. It is the ability to frequently cut itself from the linear continuity of the chromosome by introducing double-strand breaks (DSBs) that makes Ac a powerful mutagen. The subsequent repair of these DSBs by the host’s enzymatic machinery rarely leaves the DNA in ...
Assessing the biocompatibility of click
... approach would not only eliminate the need for enzymatic ligation and cloning during gene synthesis to enable the full automation of large-scale gene synthesis, but also readily allow the incorporation of modified bases into large DNA fragments. The resulting click-linked DNA will however, contain an ...
... approach would not only eliminate the need for enzymatic ligation and cloning during gene synthesis to enable the full automation of large-scale gene synthesis, but also readily allow the incorporation of modified bases into large DNA fragments. The resulting click-linked DNA will however, contain an ...
LETTERS
... protein, with vaccinia TopIB (which is insensitive to topotecan) or with a nick induced by a nicking enzyme (Supplementary Information V). As expected, these experiments show that catalytically active human TopIB is required to yield slow uncoiling, consistent with the in vivo activity and co-crysta ...
... protein, with vaccinia TopIB (which is insensitive to topotecan) or with a nick induced by a nicking enzyme (Supplementary Information V). As expected, these experiments show that catalytically active human TopIB is required to yield slow uncoiling, consistent with the in vivo activity and co-crysta ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.