Genetics
... Adenine always base pairs with Thymine (or Uracil if RNA) Cytosine always base pairs with Guanine. This is beacuse there is exactly enough room for one purine and ...
... Adenine always base pairs with Thymine (or Uracil if RNA) Cytosine always base pairs with Guanine. This is beacuse there is exactly enough room for one purine and ...
Ciliogenesis and the DNA damage response: a stressful relationship
... localisation and a major nuclear component. The latter functionally responds to UV-induced DNA damage and physically interacts with XPC to promote efficient repair of UV-induced DNA lesions [52–54]. Recent studies suggest that ATM can also act as a versatile protein kinase during cytoplasmic signall ...
... localisation and a major nuclear component. The latter functionally responds to UV-induced DNA damage and physically interacts with XPC to promote efficient repair of UV-induced DNA lesions [52–54]. Recent studies suggest that ATM can also act as a versatile protein kinase during cytoplasmic signall ...
Full-Text PDF
... 2 µm (geologists), or 1 µm (colloid chemists) are defined as clay particles. All clays are generally plastic if they contain water and they harden if they dry or when fired. This principle is applied in the clay industry. The plasticity might even be influenced by microorganisms. Many well-known met ...
... 2 µm (geologists), or 1 µm (colloid chemists) are defined as clay particles. All clays are generally plastic if they contain water and they harden if they dry or when fired. This principle is applied in the clay industry. The plasticity might even be influenced by microorganisms. Many well-known met ...
Stereoselectivity in DNA-Templated Organic
... abasic region instead of from the 5′ end also induced stereoselectivity only after several bases were restored (5-11 bases, in this ...
... abasic region instead of from the 5′ end also induced stereoselectivity only after several bases were restored (5-11 bases, in this ...
all atom and coarse grained dna simulation studies
... non-bonded interactions have a nonzero value even for large inter-particle distances. This implies a huge number of pairwise calculations and in practice we can not compute them all. For a system involving N particles combinatorics tells us that the number of distinct particle pairs is equal to N (N ...
... non-bonded interactions have a nonzero value even for large inter-particle distances. This implies a huge number of pairwise calculations and in practice we can not compute them all. For a system involving N particles combinatorics tells us that the number of distinct particle pairs is equal to N (N ...
Caitlin Davis
... Plasmids are small circular pieces of DNA found in bacteria. They are commonly used in biotechnology as vectors: DNA that can accept, carry, and replicate other pieces of DNA (Thieman and Palladino, 2009). Restriction enzymes are enzymes that recognize segments of DNA, called restriction sites, and ...
... Plasmids are small circular pieces of DNA found in bacteria. They are commonly used in biotechnology as vectors: DNA that can accept, carry, and replicate other pieces of DNA (Thieman and Palladino, 2009). Restriction enzymes are enzymes that recognize segments of DNA, called restriction sites, and ...
Eukaryotic DNA Replication
... The key event in controlling replication is the behavior of the ORC complex at the origin. The origin (ARS) consists of the A consensus sequence and three B elements. The ORC complex of six proteins binds to the A and adjacent B1 element. The transcription factor ABF1 binds to the B3 element; thi ...
... The key event in controlling replication is the behavior of the ORC complex at the origin. The origin (ARS) consists of the A consensus sequence and three B elements. The ORC complex of six proteins binds to the A and adjacent B1 element. The transcription factor ABF1 binds to the B3 element; thi ...
Platinum DNA polymerases
... Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficu ...
... Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficu ...
High-Throughput Analysis of Foodborne Bacterial Genomic DNA
... and DNA single-(ss) and double-stranded (ds), of any size, will contribute to UV-absorbance at 260 nm. However, for DNA fragmentation and library construction steps, input of specific quantities of HMW double-stranded DNA are required. Fluorescence-based methods using an intercalated dye are commonl ...
... and DNA single-(ss) and double-stranded (ds), of any size, will contribute to UV-absorbance at 260 nm. However, for DNA fragmentation and library construction steps, input of specific quantities of HMW double-stranded DNA are required. Fluorescence-based methods using an intercalated dye are commonl ...
S-Phase Checkpoint Genes Safeguard High
... yeast (Lisby et al., 2001; Melo and Toczyski, 2002). Interestingly, foci containing some of the same repair proteins are detectable during S phase in human cells that have not been subjected to DNA-damaging agents (Costanzo et al., 2001; Maser et al., 2001; Mirzoeva and Petrini, 2003), suggesting th ...
... yeast (Lisby et al., 2001; Melo and Toczyski, 2002). Interestingly, foci containing some of the same repair proteins are detectable during S phase in human cells that have not been subjected to DNA-damaging agents (Costanzo et al., 2001; Maser et al., 2001; Mirzoeva and Petrini, 2003), suggesting th ...
to 3 - NUAMESAPBio
... Synthesizing a New DNA Strand ▪ DNA polymerases cannot initiate synthesis of a polynucleotide; they can only add nucleotides to an already existing chain base-paired with the template ▪ The initial nucleotide strand is a short RNA primer ▪ The enzyme, primase, starts an RNA chain from a single RNA ...
... Synthesizing a New DNA Strand ▪ DNA polymerases cannot initiate synthesis of a polynucleotide; they can only add nucleotides to an already existing chain base-paired with the template ▪ The initial nucleotide strand is a short RNA primer ▪ The enzyme, primase, starts an RNA chain from a single RNA ...
Single-stranded DNA-binding Proteins
... role is to enzymatically catalyse homologous recombination for DNA repair. RecA homologues are ubiquitous and have been found in minimalist organisms, such as bacteriophage T4 (UvsX) and Mycoplasma, to eukaryotes including yeast (RAD51) and humans (hRad51), as well as over 60 bacterial species (Roca ...
... role is to enzymatically catalyse homologous recombination for DNA repair. RecA homologues are ubiquitous and have been found in minimalist organisms, such as bacteriophage T4 (UvsX) and Mycoplasma, to eukaryotes including yeast (RAD51) and humans (hRad51), as well as over 60 bacterial species (Roca ...
dna: the indispensible forensic science tool
... • Four types of bases are associated with the DNA structure: adenine (A), guanine (G), cytosine (C), and thymine (T). • The bases on each strand are properly aligned in a double-helix configuration, which is two strands of DNA coiled together. • As a result, adenine pairs with thymine and guanine pa ...
... • Four types of bases are associated with the DNA structure: adenine (A), guanine (G), cytosine (C), and thymine (T). • The bases on each strand are properly aligned in a double-helix configuration, which is two strands of DNA coiled together. • As a result, adenine pairs with thymine and guanine pa ...
Highly Sensitive and Robust Automated DNA Extraction from
... Viral RNA Mini QIAcube Kit on the QIAcube Instrument (Qiagen, Hilden, Germany) was used as the reference method. Each run of 90 min included: 10 samples, a positive control (~ 90 mg/ml CHO DNA in TE buffer), and a negative control (TE buffer only). Sample and elution volumes were 140 µl each. Carrie ...
... Viral RNA Mini QIAcube Kit on the QIAcube Instrument (Qiagen, Hilden, Germany) was used as the reference method. Each run of 90 min included: 10 samples, a positive control (~ 90 mg/ml CHO DNA in TE buffer), and a negative control (TE buffer only). Sample and elution volumes were 140 µl each. Carrie ...
Using DNA to solve the Bounded Post Correspondence Problem
... notions. For further details of molecular biology terminology, the reader is referred to [32]. DNA (deoxyribonucleic acid) is found in every cellular organism as the storage medium for genetic information. It is composed of units called nucleotides, distinguished by the chemical group, or base, atta ...
... notions. For further details of molecular biology terminology, the reader is referred to [32]. DNA (deoxyribonucleic acid) is found in every cellular organism as the storage medium for genetic information. It is composed of units called nucleotides, distinguished by the chemical group, or base, atta ...
How Can Transposons Accelerate Your Genomics
... – Optimize electroporation conditions using plasmid DNA (as a starting point, try 50 µL cells, 1 µL Transposome, 2 mm cuvette, 2500V, 5 msec time constant). – Recover cells immediately after electroporation. ...
... – Optimize electroporation conditions using plasmid DNA (as a starting point, try 50 µL cells, 1 µL Transposome, 2 mm cuvette, 2500V, 5 msec time constant). – Recover cells immediately after electroporation. ...
An Approximate Approach to DNA Denaturation
... Let us briefly review the development of experimental work t h a t has been done in recent years. The experimental background comes mainly from R a m a n and IR spectroscopic results t h a t are an alternative approach (Urabe and Tominaga 1981), more closely connected to theoretical lattice dynamics ...
... Let us briefly review the development of experimental work t h a t has been done in recent years. The experimental background comes mainly from R a m a n and IR spectroscopic results t h a t are an alternative approach (Urabe and Tominaga 1981), more closely connected to theoretical lattice dynamics ...
Improvement of DNA Extraction Protocols for Nostochopsis spp.
... improvement of DNA extraction from this cyanobacterium, including; crushing with glass beads and liquid nitrogen, washing with 3 M NaCl and crushing with glass beads, freezingthawing, crushing with glass beads and sonication, crushing with liquid nitrogen and adding polyvinylpolypyrrolidone (PVPP), ...
... improvement of DNA extraction from this cyanobacterium, including; crushing with glass beads and liquid nitrogen, washing with 3 M NaCl and crushing with glass beads, freezingthawing, crushing with glass beads and sonication, crushing with liquid nitrogen and adding polyvinylpolypyrrolidone (PVPP), ...
BioTeke Corporation Technical Manual
... centrifuged. DNA binds to the silicified membrane while contaminants such as proteins and polysaccharides are efficiently removed by two-step wash. Purified DNA is eluted in a small volume of low ionic strength buffer or water. ...
... centrifuged. DNA binds to the silicified membrane while contaminants such as proteins and polysaccharides are efficiently removed by two-step wash. Purified DNA is eluted in a small volume of low ionic strength buffer or water. ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.