DNA Metallization Processes and Nanoelectronics
... follows from these studies that when DNA is incubated with Pt(II) complexes such as cisplatin, the Pt(II) atom binds to one- or two-stacked DNA bases forming monofunctional and bifunctional DNA-Pt(II) adducts, respectively. The most favorable binding site for cisplatin to the DNA is the N7 position ...
... follows from these studies that when DNA is incubated with Pt(II) complexes such as cisplatin, the Pt(II) atom binds to one- or two-stacked DNA bases forming monofunctional and bifunctional DNA-Pt(II) adducts, respectively. The most favorable binding site for cisplatin to the DNA is the N7 position ...
Corchorus yellow vein virus, a New World geminivirus from the Old
... the remaining sequence of DNA A and DNA B from the virus infecting Jute, outwardly extending specific primers (DNA A: 201For 59-TCCTCTTCGAAGAACTCCT-39, 201Rev 59-TGTATGAGCAATATCGTGAC-39; DNA B: 201BFor 59-GAAGGTATGATGTCTTCCTG-39, 201BRev 59-AATCACAATTAGCTCAAGC-39) were used in PCRs comprising a 1 ml ...
... the remaining sequence of DNA A and DNA B from the virus infecting Jute, outwardly extending specific primers (DNA A: 201For 59-TCCTCTTCGAAGAACTCCT-39, 201Rev 59-TGTATGAGCAATATCGTGAC-39; DNA B: 201BFor 59-GAAGGTATGATGTCTTCCTG-39, 201BRev 59-AATCACAATTAGCTCAAGC-39) were used in PCRs comprising a 1 ml ...
pdf, 1.3 MB - DNA and Natural Algorithms Group
... The process of replication is complete by this point; we started with two parental superstrands, and we now have two additional daughter superstrands. Just as DNA, replication was semi-conservative; each of the superduplexes contain one parental strand and one daughter strand. All that remains to be ...
... The process of replication is complete by this point; we started with two parental superstrands, and we now have two additional daughter superstrands. Just as DNA, replication was semi-conservative; each of the superduplexes contain one parental strand and one daughter strand. All that remains to be ...
A comprehensive computational model of facilitated diffusion in
... The orientation of TFs affects the affinity of the TF for a specific position on the DNA, i.e. a molecule bound in one orientation can have a totally different affinity compared with being bound in the opposite orientation at the same position. Finally, since transcription and translation are co-loc ...
... The orientation of TFs affects the affinity of the TF for a specific position on the DNA, i.e. a molecule bound in one orientation can have a totally different affinity compared with being bound in the opposite orientation at the same position. Finally, since transcription and translation are co-loc ...
Archaeal DNA Replication:A Robust Model for Eukaryotes
... proposed that key initiator proteins interact with sites within a DNA molecule whereupon they lead to the initiation of DNA replication. Thus, replication initiates at defined locations in a given DNA molecule. There is a fundamental difference in the organisation of replication origins between bacte ...
... proposed that key initiator proteins interact with sites within a DNA molecule whereupon they lead to the initiation of DNA replication. Thus, replication initiates at defined locations in a given DNA molecule. There is a fundamental difference in the organisation of replication origins between bacte ...
Recombination - Transformation
... The suppression of homologous recombination started outside of the SRY (Sex Reversal of Y) gene and spread to other regions in a rather spontaneous fashion, leading to the loss of genes and chromatin. It has been speculated that the cause of suppression is inversion on the Y-chromosome. When the hom ...
... The suppression of homologous recombination started outside of the SRY (Sex Reversal of Y) gene and spread to other regions in a rather spontaneous fashion, leading to the loss of genes and chromatin. It has been speculated that the cause of suppression is inversion on the Y-chromosome. When the hom ...
Real Time of PCR - KSU Faculty Member websites
... procedure now, and its use is extremely wide-ranging. At its most basic application, PCR can amplify a small amount of template DNA (or RNA) into large quantities in a few hours. This is performed by mixing the DNA with primers on either side of the DNA (forward and reverse), Taq polymerase (of the ...
... procedure now, and its use is extremely wide-ranging. At its most basic application, PCR can amplify a small amount of template DNA (or RNA) into large quantities in a few hours. This is performed by mixing the DNA with primers on either side of the DNA (forward and reverse), Taq polymerase (of the ...
Checkpoint responses to unusual structures formed by DNA repeats
... repeats with imperfect stems such as (CNG)n repeats cannot form DNA cruciforms as the branch migration process is blocked even by a single base mismatch [22]. The existence of DNA cruciforms in vivo has been proven in bacterial cells under conditions that increase negative superhelicity, such as tra ...
... repeats with imperfect stems such as (CNG)n repeats cannot form DNA cruciforms as the branch migration process is blocked even by a single base mismatch [22]. The existence of DNA cruciforms in vivo has been proven in bacterial cells under conditions that increase negative superhelicity, such as tra ...
Student`s guide -
... restriction enzymes. They are so called because they are made by bacteria to restrict the proliferation of viruses that attack them (the enzymes do this by cutting up the viral DNA). Restriction enzymes take their names from the bacterial species that produce them. For example, BamHI is obtained fro ...
... restriction enzymes. They are so called because they are made by bacteria to restrict the proliferation of viruses that attack them (the enzymes do this by cutting up the viral DNA). Restriction enzymes take their names from the bacterial species that produce them. For example, BamHI is obtained fro ...
Genes without frontiers?
... whole plasmids into bacterial chromosomes at a 100-fold greater rate than foreign DNA uptake from the same plasmid when linearised. Several studies estimate the degree of similarity required for homologous recombination in different types of bacteria. The frequency of recombination of DNA molecules ...
... whole plasmids into bacterial chromosomes at a 100-fold greater rate than foreign DNA uptake from the same plasmid when linearised. Several studies estimate the degree of similarity required for homologous recombination in different types of bacteria. The frequency of recombination of DNA molecules ...
How Does Replication-Associated Mutational Pressure Influence
... In fast-dividing cells, the copy number of proximal genes can be up to eight times higher than that of distal genes (Cooper and Helmstetter 1968). This reflects the topology of replication when the cell cycle is shorter than the time needed for replication of the whole chromosome. Nevertheless, it i ...
... In fast-dividing cells, the copy number of proximal genes can be up to eight times higher than that of distal genes (Cooper and Helmstetter 1968). This reflects the topology of replication when the cell cycle is shorter than the time needed for replication of the whole chromosome. Nevertheless, it i ...
We are interested in computational problems motivated by
... acid, DNA. DNA js a very large molecule, and is made of srllull mokculr=s culled nelrlceotides. It consist of two coinplcmcntary chains twisted about each othcr in the form of a double fielix. Each chain is cornposcd of four nucleotides that contain a deoxyribose residue, a phosphate, and a pyrnmidi ...
... acid, DNA. DNA js a very large molecule, and is made of srllull mokculr=s culled nelrlceotides. It consist of two coinplcmcntary chains twisted about each othcr in the form of a double fielix. Each chain is cornposcd of four nucleotides that contain a deoxyribose residue, a phosphate, and a pyrnmidi ...
AUTOMATED DNA SEQUENCING, MegaBACE 1000
... fragment sizes from 25 base pairs up to 1000 bases are produced by the sequencing chemistry with each fragment containing a single fluorescent label that corresponds to the last DNA letter of the fragment and by matching the color to size the original DNA sequence can be easily determined. (Hawkins ...
... fragment sizes from 25 base pairs up to 1000 bases are produced by the sequencing chemistry with each fragment containing a single fluorescent label that corresponds to the last DNA letter of the fragment and by matching the color to size the original DNA sequence can be easily determined. (Hawkins ...
Multiple PCR analyses on trace amounts of DNA
... by this method into a template pool. A minute fraction of this pool contains sufficient DNA for a number of specific PCR analyses. One major concern about PCR random amplification of trace amounts of DNA is the fidelity of the amplified products. To achieve good fidelity it is critical to minimise e ...
... by this method into a template pool. A minute fraction of this pool contains sufficient DNA for a number of specific PCR analyses. One major concern about PCR random amplification of trace amounts of DNA is the fidelity of the amplified products. To achieve good fidelity it is critical to minimise e ...
Summary and Discussion English
... Conservation of genomic integrity is essential for correct expression of the genome and for the faithful transmission of genetic information to the next generations. However, all living organisms are continuously exposed to a variety of endogenous and environmental DNA-damaging agents, which threat ...
... Conservation of genomic integrity is essential for correct expression of the genome and for the faithful transmission of genetic information to the next generations. However, all living organisms are continuously exposed to a variety of endogenous and environmental DNA-damaging agents, which threat ...
Rapid Purification of DNA with High PCR Efficiency
... the Materials section. The DNA was then subjected to PCR using E.coli-specific primers which recognize the 16S rRNA gene. The resulting PCR products were run on a 1% agarose gel for visualization, and can be seen in Panel A above. The number of E. coli cells used to spike the milk is indicated abov ...
... the Materials section. The DNA was then subjected to PCR using E.coli-specific primers which recognize the 16S rRNA gene. The resulting PCR products were run on a 1% agarose gel for visualization, and can be seen in Panel A above. The number of E. coli cells used to spike the milk is indicated abov ...
Pyrosequencing Technology
... Pyrosequencing GmbH. Germany Pyrosequencing AB. Nordic region Pyrosequencing Ltd. UK & Eire ...
... Pyrosequencing GmbH. Germany Pyrosequencing AB. Nordic region Pyrosequencing Ltd. UK & Eire ...
Dr. Assal - World Health Organization
... • Standardization of the different steps, from sample preparation to amplification and ...
... • Standardization of the different steps, from sample preparation to amplification and ...
Unit 3 - OpenWetWare
... method with laser detection. • ddNTPs, dNTPs, primers, DNA polymerase, and the DNA of interest were mixed in a single reaction tube. However the ddNTPs and primer were labelled with a fluorescent dye. • Instead of gel electrophoresis, the reaction products were put into a single lane tube of gel cal ...
... method with laser detection. • ddNTPs, dNTPs, primers, DNA polymerase, and the DNA of interest were mixed in a single reaction tube. However the ddNTPs and primer were labelled with a fluorescent dye. • Instead of gel electrophoresis, the reaction products were put into a single lane tube of gel cal ...
Active-site Determination of a Pyrimidine Dimer
... that seen for the cis-syn CPD; however, the trapping appears to be more ef®cient for both of these enzymes on the AP site-containing DNA. However, the T2P cv-pdg (lane 9) mutant appeared to be the most readily formed enzyme substrate complex on AP site-containing DNA. ...
... that seen for the cis-syn CPD; however, the trapping appears to be more ef®cient for both of these enzymes on the AP site-containing DNA. However, the T2P cv-pdg (lane 9) mutant appeared to be the most readily formed enzyme substrate complex on AP site-containing DNA. ...
The replication of DNA
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
CHAPTER 8 Recombinant DNA Technology
... 6. All copies of a chromosome will contain the same restriction sites, and will be cut into identical fragments. 7. Based on probability, a specific short DNA sequence occurs more frequently than a longer one. a. In a 50% G-C organism with random distribution of bases, the probability of a specific ...
... 6. All copies of a chromosome will contain the same restriction sites, and will be cut into identical fragments. 7. Based on probability, a specific short DNA sequence occurs more frequently than a longer one. a. In a 50% G-C organism with random distribution of bases, the probability of a specific ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.