Discovering DNA: Structure and Replication
... • scientists thought a protein • others that it was a nucleic acid. • Three major experiments helped shows nucleic acid carried cell information: – Griffith – Avery – Hershey-Chase ...
... • scientists thought a protein • others that it was a nucleic acid. • Three major experiments helped shows nucleic acid carried cell information: – Griffith – Avery – Hershey-Chase ...
DNA Structure and Replication
... 2. DNA is a nucleic acid consisting of two long strands of covalently bonded nucleotides, held to each other by hydrogen bonds between complementary nitrogen bases. 3. A nucleotide contains deoxyribose sugar, a phosphate group, and a nitrogen base (adenine, guanine, cytosine or thymine). 4. The doub ...
... 2. DNA is a nucleic acid consisting of two long strands of covalently bonded nucleotides, held to each other by hydrogen bonds between complementary nitrogen bases. 3. A nucleotide contains deoxyribose sugar, a phosphate group, and a nitrogen base (adenine, guanine, cytosine or thymine). 4. The doub ...
PCR denaturation temperature 94C The hydrogen bonds are broken
... The hydrogen bonds are broken in the double stranded DNA, creating single strands of DNA that are susceptible to copying. ...
... The hydrogen bonds are broken in the double stranded DNA, creating single strands of DNA that are susceptible to copying. ...
DNA Structure
... Genes code for traits such as: ◦ Hair color ◦ Eye Color ◦ How many fingers you have ◦ Skin color ◦ Enzymes to break down the food that you eat ...
... Genes code for traits such as: ◦ Hair color ◦ Eye Color ◦ How many fingers you have ◦ Skin color ◦ Enzymes to break down the food that you eat ...
DNA
... • DNA is found in the mitochondria. • mDNA is only found in the egg. Sperm has no mitochondria so mDNA is passed to offspring from the mother. • One sequence of DNA is a genome or gene. • Unwind all our DNA, it will stretch from the moon and back 6000X. ...
... • DNA is found in the mitochondria. • mDNA is only found in the egg. Sperm has no mitochondria so mDNA is passed to offspring from the mother. • One sequence of DNA is a genome or gene. • Unwind all our DNA, it will stretch from the moon and back 6000X. ...
Isolation of DNA from 96 Well Plates
... 2. Add 50μl of Lysis Buffer containing Proteinase-K to the empty wells. 3. To prevent evaporation, tape lid down using tape seals (roll across plate to form a seal, watch for bubbles around edge that may cause wells to dry out). 4. Incubate overnight at 55oC. Day 2 5. Spin down plate for 3-5 minutes ...
... 2. Add 50μl of Lysis Buffer containing Proteinase-K to the empty wells. 3. To prevent evaporation, tape lid down using tape seals (roll across plate to form a seal, watch for bubbles around edge that may cause wells to dry out). 4. Incubate overnight at 55oC. Day 2 5. Spin down plate for 3-5 minutes ...
Strawberry DNA Extraction Adapted from a lab by C. Sheldon
... 8. Slowly pour the ice-cold alcohol into the tube until the tube is half full and forms a layer over the top of the strawberry extract. 9. At the interface, you will see the DNA precipitate out of solution and float to the top. You may spool the DNA on your glass rod or pipette tip. 10. Spool the DN ...
... 8. Slowly pour the ice-cold alcohol into the tube until the tube is half full and forms a layer over the top of the strawberry extract. 9. At the interface, you will see the DNA precipitate out of solution and float to the top. You may spool the DNA on your glass rod or pipette tip. 10. Spool the DN ...
HW2 DNA and Replication - Liberty Union High School District
... 10. Purines have _________ rings, and pyrimidines have ____________ ring. 11. Chargaff's rule states that the DNA of any species contains equal amounts of ____________________________ & _______________________ and also equal amounts of _______________________ & _______________________ 12. In DNA, th ...
... 10. Purines have _________ rings, and pyrimidines have ____________ ring. 11. Chargaff's rule states that the DNA of any species contains equal amounts of ____________________________ & _______________________ and also equal amounts of _______________________ & _______________________ 12. In DNA, th ...
Concept guide for Exam #3 Bio310 F`07
... information storage molecule? Why can new nucleotides only be added to the 3’ end of a strand of DNA? Be able to draw a DNA replication fork. Why must there be a lagging strand in DNA replication Why are the two DNA Pol III’s kept together in one complex? Know the function of the starred proteins in ...
... information storage molecule? Why can new nucleotides only be added to the 3’ end of a strand of DNA? Be able to draw a DNA replication fork. Why must there be a lagging strand in DNA replication Why are the two DNA Pol III’s kept together in one complex? Know the function of the starred proteins in ...
Deoxyribonucleic acid from calf thymus (D4522)
... The Activated Calf Thymus DNA is prepared by modification of a published method using calf thymus DNA (Product No. D 1501) and DNase I (Product No. ...
... The Activated Calf Thymus DNA is prepared by modification of a published method using calf thymus DNA (Product No. D 1501) and DNase I (Product No. ...
DNA Presentation
... DNA has 2 complementary strands. Each base pairs up with another complementary base on the other strand. The DNA molecule untwists, unzips or splits down the middle, and then an enzyme pairs new bases up and creates 2 identical strands. ...
... DNA has 2 complementary strands. Each base pairs up with another complementary base on the other strand. The DNA molecule untwists, unzips or splits down the middle, and then an enzyme pairs new bases up and creates 2 identical strands. ...
J) DNA_Workshop_webquest2
... When making a protein, a cell only needs to use a portion of the DNA strand. The first step in protein synthesis is called transcription. a. In the lower right hand corner, where is transcription occurring?_________________________________ Drag nucleotide bases to make a complementary of the DNA. Th ...
... When making a protein, a cell only needs to use a portion of the DNA strand. The first step in protein synthesis is called transcription. a. In the lower right hand corner, where is transcription occurring?_________________________________ Drag nucleotide bases to make a complementary of the DNA. Th ...
Transgenic Organisms
... 2. Whatever gene is taken up is then expressed by the plant cell 3. What are some advantages and disadvantages of this technology? ...
... 2. Whatever gene is taken up is then expressed by the plant cell 3. What are some advantages and disadvantages of this technology? ...
DNA REVIEW SHEET
... 16. A codon that has no anticodon match would be called a ___________________. 17. What does DNA polymerase do? 18. Anything ending in –ase would be classified as an ____________________> 19. What 3 things make up DNA? 20. DNA is compared in structure to what? 21. What does DNA stand for? 22. How ma ...
... 16. A codon that has no anticodon match would be called a ___________________. 17. What does DNA polymerase do? 18. Anything ending in –ase would be classified as an ____________________> 19. What 3 things make up DNA? 20. DNA is compared in structure to what? 21. What does DNA stand for? 22. How ma ...
DNA - The Double Helix
... phosphate molecules. The sugar is a pentose called deoxyribose. Color all the phosphates (one is labeled with a "p"). Color all the deoxyribose sugars (one is labeled with a "D"). What is meant by a double helix? ____________________________ Name a pentose sugar. _________________ The sides of DNA a ...
... phosphate molecules. The sugar is a pentose called deoxyribose. Color all the phosphates (one is labeled with a "p"). Color all the deoxyribose sugars (one is labeled with a "D"). What is meant by a double helix? ____________________________ Name a pentose sugar. _________________ The sides of DNA a ...
DNAtheDoubleHelix
... and phosphate molecules. The sugar is a pentose called deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the deoxyribose sugars blue (one is labeled with a "D"). What is meant by a double helix? ____________________________ Name a pentose sugar. _________________ The ...
... and phosphate molecules. The sugar is a pentose called deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the deoxyribose sugars blue (one is labeled with a "D"). What is meant by a double helix? ____________________________ Name a pentose sugar. _________________ The ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.