Worksheet – Structure of DNA and Replication
... Directions: Complete each sentence. 7. Guanine, cytosine, thymine, and __________________ are the four __________________ in DNA. 8. In DNA, guanine always forms hydrogen bonds with __________________. 9. The process of __________________ produces a new copy of an organism’s genetic information, wh ...
... Directions: Complete each sentence. 7. Guanine, cytosine, thymine, and __________________ are the four __________________ in DNA. 8. In DNA, guanine always forms hydrogen bonds with __________________. 9. The process of __________________ produces a new copy of an organism’s genetic information, wh ...
Enterococcus faecalis VRE, Genomic DNA
... was extracted from the cells following a modified bacterial protocol from the Qiagen® Genomic DNA Handbook using ...
... was extracted from the cells following a modified bacterial protocol from the Qiagen® Genomic DNA Handbook using ...
Lesson 1
... Four different bases give rise to four different nucleotides in DNA. -bases: adenine (A), guanine (G), cytosine (C), and thymine (T) -pairing of bases: A with T G with C ...
... Four different bases give rise to four different nucleotides in DNA. -bases: adenine (A), guanine (G), cytosine (C), and thymine (T) -pairing of bases: A with T G with C ...
Expanding the DNA alphabet: `Extra` DNA base found to
... physical position in the genome makes it likely that in living tissue, making it likely that it plays a key it plays a key role in gene activity. role in the genome. "This modification to DNA is found in very specific positions in the genome—the places which regulate genes," said the paper's lead au ...
... physical position in the genome makes it likely that in living tissue, making it likely that it plays a key it plays a key role in gene activity. role in the genome. "This modification to DNA is found in very specific positions in the genome—the places which regulate genes," said the paper's lead au ...
Prepare for gel electrophoresis
... (mature red blood cells have no nucleus). Mitochondrion have a totally different set of genes than what is in the nucleus.You inherit them ...
... (mature red blood cells have no nucleus). Mitochondrion have a totally different set of genes than what is in the nucleus.You inherit them ...
Lecture 4
... Human Genome Project only had to sequence 3.2 billion 1 cell: 2 copies of each chromosome, but can throw out 1 copy, then halve the other copy So, 3.2 billion nucleotides reflects ¼ of the nucleotides found in a cell Project was a huge undertaking; storing data and searching it is difficult ...
... Human Genome Project only had to sequence 3.2 billion 1 cell: 2 copies of each chromosome, but can throw out 1 copy, then halve the other copy So, 3.2 billion nucleotides reflects ¼ of the nucleotides found in a cell Project was a huge undertaking; storing data and searching it is difficult ...
Teaching Notes
... helix, it is right handed and if you wrap the helix with your left hand then it is left handed. ...
... helix, it is right handed and if you wrap the helix with your left hand then it is left handed. ...
„DNA damage“?
... • initiated by DNA glycosylases, which recognize damaged bases and excise them from DNA by hydrolyzing the Nglycosidic bond • substrate specificity of the glycosylases: developed to repair expectable „errors“? • second enzyme is AP-lyase introducing single strand break next to the abasic ...
... • initiated by DNA glycosylases, which recognize damaged bases and excise them from DNA by hydrolyzing the Nglycosidic bond • substrate specificity of the glycosylases: developed to repair expectable „errors“? • second enzyme is AP-lyase introducing single strand break next to the abasic ...
DNA: The Genetic Material
... • Partial opening of helix forms replication fork • DNA primase – RNA polymerase that makes RNA primer – RNA will be removed and replaced with DNA ...
... • Partial opening of helix forms replication fork • DNA primase – RNA polymerase that makes RNA primer – RNA will be removed and replaced with DNA ...
DNA
... These Complimentary Base Pairs join together via hydrogen bonds to form the double-helix shape that we all know and love. ...
... These Complimentary Base Pairs join together via hydrogen bonds to form the double-helix shape that we all know and love. ...
Goal 3.01 Quiz 1
... A. so that bonds break for translation to occur B. so that bonds break for DNA replication to occur C. so that DNA can twist to form the double helix shape D. so that DNA can be strengthened by another bond during DNA replication ...
... A. so that bonds break for translation to occur B. so that bonds break for DNA replication to occur C. so that DNA can twist to form the double helix shape D. so that DNA can be strengthened by another bond during DNA replication ...
Document
... Take any plasmid in which the gene of interest is inserted. Multiply this plasmid within a methylating bacteria. (While plasmid DNA isolated from almost all of the commonly usedE. coli strains (dam+) is methylated and is a suitable template formutagenesis, plasmid DNA isolated from the exceptional d ...
... Take any plasmid in which the gene of interest is inserted. Multiply this plasmid within a methylating bacteria. (While plasmid DNA isolated from almost all of the commonly usedE. coli strains (dam+) is methylated and is a suitable template formutagenesis, plasmid DNA isolated from the exceptional d ...
Extra Credit DNA Study Guide
... 53. List in order the steps scientists need to do to add the gene to make insulin into bacteria. (pg 327-328). 1. Add a genetic marker such as a florescent protein tag or an antibiotic resistant tag. 2. Extract the insulin protein from the bacterial culture. 3. Transform the bacteria with the recomb ...
... 53. List in order the steps scientists need to do to add the gene to make insulin into bacteria. (pg 327-328). 1. Add a genetic marker such as a florescent protein tag or an antibiotic resistant tag. 2. Extract the insulin protein from the bacterial culture. 3. Transform the bacteria with the recomb ...
DNA – The Double Helix In 1952, Rosalind Franklin discovered that
... In 1952, Rosalind Franklin discovered that DNA (deoxyribonucleic acid) is two chains of molecules in a spiral form. In 1953, using the work of Franklin and other scientists, James Watson and Francis Crick established the structure of DNA. They determined that the shape of DNA is a double helix, whic ...
... In 1952, Rosalind Franklin discovered that DNA (deoxyribonucleic acid) is two chains of molecules in a spiral form. In 1953, using the work of Franklin and other scientists, James Watson and Francis Crick established the structure of DNA. They determined that the shape of DNA is a double helix, whic ...
Genetic Technology
... How do we make changes to DNA of an organism??? USING….Restriction Enzymes: bacterial enzymes act like scissors that cut DNA molecules at specific sites create sticky ends that can easily bind to complementary DNA and form new sequences of nucleotides ...
... How do we make changes to DNA of an organism??? USING….Restriction Enzymes: bacterial enzymes act like scissors that cut DNA molecules at specific sites create sticky ends that can easily bind to complementary DNA and form new sequences of nucleotides ...
BIO I Review Packet Protein Synthesis 2017
... 8. What type of bond holds together the “backbone” of DNA? 9. What type of bond holds together the two strands of DNA? 10. DNA has the instructions for making? _________________________ ...
... 8. What type of bond holds together the “backbone” of DNA? 9. What type of bond holds together the two strands of DNA? 10. DNA has the instructions for making? _________________________ ...
Section 6.2 Questions, page 279 1. If Hershey and Chase had found
... nitrogenous base. (b) Purines are a class of nitrogenous bases with a double ring structure; adenine and guanine are purines. Pyrimidines are a class of nitrogenous bases with a single ring structure; thymine and cytosine are pyrimidines. (c) Bacteria are prokaryotic single-celled organisms. Bacteri ...
... nitrogenous base. (b) Purines are a class of nitrogenous bases with a double ring structure; adenine and guanine are purines. Pyrimidines are a class of nitrogenous bases with a single ring structure; thymine and cytosine are pyrimidines. (c) Bacteria are prokaryotic single-celled organisms. Bacteri ...
PCR questions
... 3. What is the purpose of heating the DNA sample to 94-96 C? 4. What is the process of adding primers called? What kind of bond forms between the primers and the DNA? ...
... 3. What is the purpose of heating the DNA sample to 94-96 C? 4. What is the process of adding primers called? What kind of bond forms between the primers and the DNA? ...
Mutations - SchneiderSBI4U
... Transposons can jump to a promoter region and either turn off or turn up transcription ...
... Transposons can jump to a promoter region and either turn off or turn up transcription ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.