GENETIC Material
... 1. What is the purpose of DNA replication? It is necessary to make a copy of DNA for a new cell that will be produced during cell division. This cell will be genetically identical to its parent cell. 2. During what stage of the cell cycle is DNA replicated? S phase (after G1, before G2) ...
... 1. What is the purpose of DNA replication? It is necessary to make a copy of DNA for a new cell that will be produced during cell division. This cell will be genetically identical to its parent cell. 2. During what stage of the cell cycle is DNA replicated? S phase (after G1, before G2) ...
DNAExtraction8 - Bakersfield College
... pure the final DNA sample must be and how accessible the DNA is within the cell. Bacterial DNA is protected only by the cell wall and cell membrane; there is no nuclear membrane as in eukaryotic cells. Therefore, the membrane can be disrupted by using dishwashing detergent which dissolves the phosph ...
... pure the final DNA sample must be and how accessible the DNA is within the cell. Bacterial DNA is protected only by the cell wall and cell membrane; there is no nuclear membrane as in eukaryotic cells. Therefore, the membrane can be disrupted by using dishwashing detergent which dissolves the phosph ...
DNA: Structure, Function, and Replication
... ● Knew DNA was composed of nitrogen base (A, T, C, G), sugar, and a phosphate group (nucleotide) ● Analyzed DNA composition of many species of organisms and came up with Chargaff’s rules: - adenine and thymine are present in roughly equal amounts - cytosine and guanine are present in roughly equal a ...
... ● Knew DNA was composed of nitrogen base (A, T, C, G), sugar, and a phosphate group (nucleotide) ● Analyzed DNA composition of many species of organisms and came up with Chargaff’s rules: - adenine and thymine are present in roughly equal amounts - cytosine and guanine are present in roughly equal a ...
Viruses and bacteria
... Viruses and bacteria Bacteria = prokaryotes = Kingdom Monera No nucleus Circular DNA (nucleoid) Reproduce by binary fission ~ 20 minutes (ideal) ...
... Viruses and bacteria Bacteria = prokaryotes = Kingdom Monera No nucleus Circular DNA (nucleoid) Reproduce by binary fission ~ 20 minutes (ideal) ...
Name: Date: Period: _____ Unit 6 (DNA, RNA, and Protein
... Can Mutations be Passed Down to Offspring? 7. Since all cells in our body contain DNA, there are a lot of places for mutations to occur ; however, some mutations cannot be passed on to offspring. A. ___________________ occur in normal body cells (non-reproductive cells) and won’t be passed on to off ...
... Can Mutations be Passed Down to Offspring? 7. Since all cells in our body contain DNA, there are a lot of places for mutations to occur ; however, some mutations cannot be passed on to offspring. A. ___________________ occur in normal body cells (non-reproductive cells) and won’t be passed on to off ...
Biol302 Spring 2011 Quiz 6
... In 1928, Griffith’s Transforming Principle revealed the “transforming” compound from the heat-killed bacteria was DNA. _____True or False_____(circle one) Question 2 5pts: Griffith’s experiment took time because transformation had to occur in this organism:________. Avery, Macleod and McCarty made t ...
... In 1928, Griffith’s Transforming Principle revealed the “transforming” compound from the heat-killed bacteria was DNA. _____True or False_____(circle one) Question 2 5pts: Griffith’s experiment took time because transformation had to occur in this organism:________. Avery, Macleod and McCarty made t ...
DNA Student Questions
... Small molecules move easily through the holes because of this, large fragments will lag behind small fragments as DNA migrates through the gel. e. Results are usually _________________ in order to save the findings. Wells are then compared together for similarities. If two fingerprints match they ar ...
... Small molecules move easily through the holes because of this, large fragments will lag behind small fragments as DNA migrates through the gel. e. Results are usually _________________ in order to save the findings. Wells are then compared together for similarities. If two fingerprints match they ar ...
DNA
... DNA bases pair via hydrogen bonds •Erwin Chargaff observed: • % adenine = % thymine •% guanine = % cytosine •Complementary bases pair: –A and T pair –C and G pair ...
... DNA bases pair via hydrogen bonds •Erwin Chargaff observed: • % adenine = % thymine •% guanine = % cytosine •Complementary bases pair: –A and T pair –C and G pair ...
Discovery of a “transforming principle”
... DNA bases pair via hydrogen bonds •Erwin Chargaff observed: • % adenine = % thymine •% guanine = % cytosine •Complementary bases pair: –A and T pair –C and G pair ...
... DNA bases pair via hydrogen bonds •Erwin Chargaff observed: • % adenine = % thymine •% guanine = % cytosine •Complementary bases pair: –A and T pair –C and G pair ...
Exam 2 Worksheet part 1 KEY
... stranded template. Primers are created by an enzyme known a primase that synthesizes short stretches of RNA complimentary to a single stranded template DNA. This creates short stretches of RNA/DNA double helix (dotted lines in the figure) with the RNA part ending in a free 3’ OH. DNA polymerase is a ...
... stranded template. Primers are created by an enzyme known a primase that synthesizes short stretches of RNA complimentary to a single stranded template DNA. This creates short stretches of RNA/DNA double helix (dotted lines in the figure) with the RNA part ending in a free 3’ OH. DNA polymerase is a ...
Microbiology bio 123
... T-A has a double H bond G-C has a triple H bond RNA 1. Two main differences between RNA and DNA 1. Uracil substitutes for Thymine, 2. Single stranded, 2. There are more than one kind of RNA, their structure is the same but their function is not. 3. Sugar base is different, still a five carbon sugar, ...
... T-A has a double H bond G-C has a triple H bond RNA 1. Two main differences between RNA and DNA 1. Uracil substitutes for Thymine, 2. Single stranded, 2. There are more than one kind of RNA, their structure is the same but their function is not. 3. Sugar base is different, still a five carbon sugar, ...
DNA These “genes” never go out of style!!
... Were also working on finding the structure of DNA through cardboard models 1953, given Franklin’s X-ray information Published findings in a historic one-page paper explaining that DNA was a double-helix in which two strands of DNA were wound around each other. Once this was discovered they exp ...
... Were also working on finding the structure of DNA through cardboard models 1953, given Franklin’s X-ray information Published findings in a historic one-page paper explaining that DNA was a double-helix in which two strands of DNA were wound around each other. Once this was discovered they exp ...
DNA replication limits…
... true of so-called spontaneous mutations. "Spontaneous" refers to the fact that the changes occur in the absence of chemical, radiation, or other environmental damage. Rather, they are usually caused by normal chemical reactions that go on in cells, such as hydrolysis. These types of errors include d ...
... true of so-called spontaneous mutations. "Spontaneous" refers to the fact that the changes occur in the absence of chemical, radiation, or other environmental damage. Rather, they are usually caused by normal chemical reactions that go on in cells, such as hydrolysis. These types of errors include d ...
swgdam 3.9 - Thermo Fisher Scientific
... • First meeting of Technical Working Group on DNA Analysis Methods (TWGDAM) was held in November 1988, with a subcommittee on restriction fragment length polymorphism (RFLP) DNA analysis. • TWGDAM continued to provide a level of direction to the forensic DNA community by issuing guidelines for DNA a ...
... • First meeting of Technical Working Group on DNA Analysis Methods (TWGDAM) was held in November 1988, with a subcommittee on restriction fragment length polymorphism (RFLP) DNA analysis. • TWGDAM continued to provide a level of direction to the forensic DNA community by issuing guidelines for DNA a ...
Ch.22Pt.2_000
... function of different types of nucleic acids •Draw the basic structure of nucleosides and nucleotides •Explain the primary structure of nucleic acids and compare it to protein structure •Describe the structural properties of the DNA double helix •Draw the steps involved in DNA replication •Compare & ...
... function of different types of nucleic acids •Draw the basic structure of nucleosides and nucleotides •Explain the primary structure of nucleic acids and compare it to protein structure •Describe the structural properties of the DNA double helix •Draw the steps involved in DNA replication •Compare & ...
DNA EVIDENCE
... Ideally rape kits should be processed right away. They should be compared to the FBI database. If a match is found, police can charge the rapist. In real life this doesn’t always happen. Forensics labs lack money for DNA testing. They don’t have enough trained staff. So rape kits collect dust on the ...
... Ideally rape kits should be processed right away. They should be compared to the FBI database. If a match is found, police can charge the rapist. In real life this doesn’t always happen. Forensics labs lack money for DNA testing. They don’t have enough trained staff. So rape kits collect dust on the ...
Genetic Engineering Notes 2017
... Increasing Variation Accomplished by inducing mutations into a population Mutations occur spontaneously, but breeders can increase the mutation rate by using radiation and chemicals. Breeders can often produce a few mutants with desirable characteristics that are not found in the original popula ...
... Increasing Variation Accomplished by inducing mutations into a population Mutations occur spontaneously, but breeders can increase the mutation rate by using radiation and chemicals. Breeders can often produce a few mutants with desirable characteristics that are not found in the original popula ...
Fulltext PDF - Indian Academy of Sciences
... blocks while sliding across the template molecule. The diameter of the polymerase enzymes and their accessory proteins is several times larger than that of double-stranded DNA. Since the process of synthesis of new RNA or DNA molecules involves tracking of such gigantic molecular complexes (,titans' ...
... blocks while sliding across the template molecule. The diameter of the polymerase enzymes and their accessory proteins is several times larger than that of double-stranded DNA. Since the process of synthesis of new RNA or DNA molecules involves tracking of such gigantic molecular complexes (,titans' ...
Part 3 - Alexander Local Schools
... I can… describe how DNA becomes the traits using RNA I can… define mutations and give 3 types I can… describe some harmful mutations in humans I can… explain the three possible outcomes of mutations. ...
... I can… describe how DNA becomes the traits using RNA I can… define mutations and give 3 types I can… describe some harmful mutations in humans I can… explain the three possible outcomes of mutations. ...
C. Nucleic acid hybridization assays using cloned target DNA, and
... d) Detection of gene deletion by restriction mapping Hetrozygotes (carriers) will display a 2:1 ratio of signal intensity for the two alleles (the full length and the shorter deleted allele). Homozygotes for the deletion will show no band for the deleted gene. ...
... d) Detection of gene deletion by restriction mapping Hetrozygotes (carriers) will display a 2:1 ratio of signal intensity for the two alleles (the full length and the shorter deleted allele). Homozygotes for the deletion will show no band for the deleted gene. ...
Learning objectives
... replication and replication forks. (Page 118) 3. Explain the role of DNA polymerases in replication.(page 118) 4. Distinguish between the leading strand and the lagging strand.(118-119) 5. Explain how the lagging strand is synthesized even though DNA polymerase can add nucleotides only to the 3’ end ...
... replication and replication forks. (Page 118) 3. Explain the role of DNA polymerases in replication.(page 118) 4. Distinguish between the leading strand and the lagging strand.(118-119) 5. Explain how the lagging strand is synthesized even though DNA polymerase can add nucleotides only to the 3’ end ...
RESTRICTION ENZYMES AND VECTORS
... Retroviral 5’LTR and 3’LTR are needed for efficient transcription of proviral DNAand for generating 3’end of length transcript. LTRs are essential for intregration of the proviral DNA into host genome. It also has R, U5, U3, P and Pu encoding sequence (involved in reverse transcription). S sequence( ...
... Retroviral 5’LTR and 3’LTR are needed for efficient transcription of proviral DNAand for generating 3’end of length transcript. LTRs are essential for intregration of the proviral DNA into host genome. It also has R, U5, U3, P and Pu encoding sequence (involved in reverse transcription). S sequence( ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.