DNA Replication – Lecture by Dr Mahmood S Choudhery
... Each new DNA molecule contains one old strand ...
... Each new DNA molecule contains one old strand ...
Supplementary Notes - Word file (74 KB )
... unlabelled ATP. The 18-mer was then annealed with a 36-mer (oligo 2: 5’TGTAGTCACTATCGGAATGAGGGCGACACGGATATG-3’) in the presence of a third 18-mer oligonucleotide (oligo 3: 5’-CATATCCGTGTCGCCCTC-3’) terminated with a 3’-dideoxy residue. All oligos were purchased from Sigma and purified by denaturing ...
... unlabelled ATP. The 18-mer was then annealed with a 36-mer (oligo 2: 5’TGTAGTCACTATCGGAATGAGGGCGACACGGATATG-3’) in the presence of a third 18-mer oligonucleotide (oligo 3: 5’-CATATCCGTGTCGCCCTC-3’) terminated with a 3’-dideoxy residue. All oligos were purchased from Sigma and purified by denaturing ...
A. thaliana genotyping with a CAPS marker for a pks3
... Arabidopsis thaliana is a small flowering plant that is widely used as a model organism in plant cellular and molecular biology. Its genome sequence is known and is available through the Arabidopsis Information Resource (TAIR), as well as other sources, including seed stocks and collections of genet ...
... Arabidopsis thaliana is a small flowering plant that is widely used as a model organism in plant cellular and molecular biology. Its genome sequence is known and is available through the Arabidopsis Information Resource (TAIR), as well as other sources, including seed stocks and collections of genet ...
chapter 16 – the molecular basis of inheritance
... B. A Closer Look at DNA Replication DNA replication is extremely accurate and efficient Although we know more about DNA replication in prokaryotes than in eukaryotes, we also know that the two processes are very similar. Six major steps of replication: a. Origins of replication: The site wher ...
... B. A Closer Look at DNA Replication DNA replication is extremely accurate and efficient Although we know more about DNA replication in prokaryotes than in eukaryotes, we also know that the two processes are very similar. Six major steps of replication: a. Origins of replication: The site wher ...
BT_Ch4_Presentation
... Gene – a section of DNA on a chromosome that contains the genetic code of a protein Nitrogenous base – an important component of nucleic acids (DNA and RNA), composed of one of two nitrogen-containing rings; forms the critical hydrogen bonds between opposing strands of a double helix Base pair – the ...
... Gene – a section of DNA on a chromosome that contains the genetic code of a protein Nitrogenous base – an important component of nucleic acids (DNA and RNA), composed of one of two nitrogen-containing rings; forms the critical hydrogen bonds between opposing strands of a double helix Base pair – the ...
Polymerase Chain Reaction (PCR) Techniques
... ClinicalRM personnel are on the cutting edge of PCR research. ClinicalRM employees have used most of the research platforms available in the modern biomedical research laboratory. Among the most used platforms are those involved with polymerase chain reactions (PCR). Recently, outbreaks of resistant ...
... ClinicalRM personnel are on the cutting edge of PCR research. ClinicalRM employees have used most of the research platforms available in the modern biomedical research laboratory. Among the most used platforms are those involved with polymerase chain reactions (PCR). Recently, outbreaks of resistant ...
Genetic alterations and DNA repair in human carcinogenesis
... errors. The mismatch is recognized, and one of the two DNA strands is selectively excised, which is followed by repair synthesis and ligation of the resulting single-stranded DNA gap. Mutation of mismatch repair genes is associated with microsatellite instability and an increased rate of somatic mut ...
... errors. The mismatch is recognized, and one of the two DNA strands is selectively excised, which is followed by repair synthesis and ligation of the resulting single-stranded DNA gap. Mutation of mismatch repair genes is associated with microsatellite instability and an increased rate of somatic mut ...
![Chapter 1 [4Fe-4S] Cluster Base Excision Repair Glycosylases](http://s1.studyres.com/store/data/006341580_1-e881f18666676a47c09b6f4d69cc6e73-300x300.png)
File
... 1. Modify DNA Gene for Protein Expression DNA cloning for protein expression requires modification of the gene using the nucleotide code for guidance. A. Modify the gene of interest so that it includes recognition sequences in the gene for restriction enzymes that would enable the gene to be insert ...
... 1. Modify DNA Gene for Protein Expression DNA cloning for protein expression requires modification of the gene using the nucleotide code for guidance. A. Modify the gene of interest so that it includes recognition sequences in the gene for restriction enzymes that would enable the gene to be insert ...
DNA Replication
... Before new DNA strands can form, there must be RNA primers present to start the addition of new nucleotides Primase is the enzyme that synthesizes the RNA Primer DNA polymerase can then add the new nucleotides ...
... Before new DNA strands can form, there must be RNA primers present to start the addition of new nucleotides Primase is the enzyme that synthesizes the RNA Primer DNA polymerase can then add the new nucleotides ...
Molecular Basis of Inheritance
... bacteria form a pellet The supernatant is radioactive, but the pellet is not. ...
... bacteria form a pellet The supernatant is radioactive, but the pellet is not. ...
Applications of Genetic Engineering
... mouse whose immune system is genetically altered to mimic some aspect of the human immune system? ...
... mouse whose immune system is genetically altered to mimic some aspect of the human immune system? ...
Chapter 10 DNA RNA Protein Synthesis
... A. Cells contain DNA that controls the production of proteins B. DNA is composed of proteins that carry coded information for how cells function C. Proteins are used to produce cells that link amino acids together into DNA D. there is no relationship between DNA, proteins, and cells ...
... A. Cells contain DNA that controls the production of proteins B. DNA is composed of proteins that carry coded information for how cells function C. Proteins are used to produce cells that link amino acids together into DNA D. there is no relationship between DNA, proteins, and cells ...
Biology
... In nature bacteria insert plasmid into plant cells that produces tumors Researchers disable tumor producing gene and introduce foreign DNA Bacteria insert the foreign DNA into the plant QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. ...
... In nature bacteria insert plasmid into plant cells that produces tumors Researchers disable tumor producing gene and introduce foreign DNA Bacteria insert the foreign DNA into the plant QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. ...
ICMP and UNMIK Announce First Joint DNA identifications in Kosovo
... PRISTINA, September 24 2002 - The Special Representative of the Secretary General to Kosovo, Mr. Michael Steiner and the Chief of Staff of the International Commission on Missing Persons (ICMP), Mr. Gordon Bacon are pleased to announce the first DNA-led identifications in Kosovo. This is the result ...
... PRISTINA, September 24 2002 - The Special Representative of the Secretary General to Kosovo, Mr. Michael Steiner and the Chief of Staff of the International Commission on Missing Persons (ICMP), Mr. Gordon Bacon are pleased to announce the first DNA-led identifications in Kosovo. This is the result ...
Designing Minor Groove Binding Drugs
... • H-bonding to base edges in the minor groove can be used to distinguish between A:T and G:C base pairs • A:T is distinguished from a T:A base pair through ...
... • H-bonding to base edges in the minor groove can be used to distinguish between A:T and G:C base pairs • A:T is distinguished from a T:A base pair through ...
Measuring forces in the DNA molecule
... However, Dietz also intends to apply the data directly to his current research: He uses DNA as programmable building material to construct machines on the order of nanometers. When doing so, he draws inspiration from the complex structures which can e.g. be found in cells and, among other things, se ...
... However, Dietz also intends to apply the data directly to his current research: He uses DNA as programmable building material to construct machines on the order of nanometers. When doing so, he draws inspiration from the complex structures which can e.g. be found in cells and, among other things, se ...
Process of Electrophoresis
... current causes the negatively-charged DNA molecules to move towards the positive electrode. Imagine the gel as a strainer with tiny pores that allow small particles to move through it very quickly. The larger the size of the particles, however, the slower they are strained through the gel. After a p ...
... current causes the negatively-charged DNA molecules to move towards the positive electrode. Imagine the gel as a strainer with tiny pores that allow small particles to move through it very quickly. The larger the size of the particles, however, the slower they are strained through the gel. After a p ...
Objective #2: Nucleic Acid Structure
... b) A drawing of each of the four types of nucleotides found in DNA (again, not all in one box!) ...
... b) A drawing of each of the four types of nucleotides found in DNA (again, not all in one box!) ...
Unit 4
... 8. Define codon, and explain what relationship exists between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide. - Each three bases of mRNA are called a codon, these codons line up with the amino acids picked up by the tRNA. 12. Explain the process of tra ...
... 8. Define codon, and explain what relationship exists between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide. - Each three bases of mRNA are called a codon, these codons line up with the amino acids picked up by the tRNA. 12. Explain the process of tra ...
Visualization of Cell-defending Nonspecific Nucleases in DNA
... cleavage but also offer a solid foundation for the better understanding of the molecular mechanisms of nonspecific endonucleases involved in the protection of bacterial cells. Protein-nucleic acid interactions play many important cellular roles, including regulating DNA replication, controlling gene ...
... cleavage but also offer a solid foundation for the better understanding of the molecular mechanisms of nonspecific endonucleases involved in the protection of bacterial cells. Protein-nucleic acid interactions play many important cellular roles, including regulating DNA replication, controlling gene ...
Paper Plasmids Lab
... Some of the most important techniques used in biotechnology today involve making recombinant DNA molecules. A recombinant object has been reassembled from parts taken from more than one source. Your genome is recombinant in that part of ,it came from your mother and part came from your father. Recom ...
... Some of the most important techniques used in biotechnology today involve making recombinant DNA molecules. A recombinant object has been reassembled from parts taken from more than one source. Your genome is recombinant in that part of ,it came from your mother and part came from your father. Recom ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.