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Cloning and PCR File
Cloning and PCR File

... 2. Annealing involves cooling the single strands of DNA and mixing them with short DNA segments called primers. Primers have base sequences that are complementary to segments of the single DNA strands. As a result, bonds form between the DNA strands and primers. 3. Extension occurs when an enzyme (T ...
DNA - Ms. Racette`s Wiki
DNA - Ms. Racette`s Wiki

Functions of DNA Polymerase e
Functions of DNA Polymerase e

... conserved in other organisms. There are two genes encoding the Pol e catalytic subunit in Arabidopsis, Pol2a and Pol2b, and single genes encoding each of the regulatory subunits. The catalytic subunit Pol2A and the regulatory subunit DPB2 in Arabidopsis are known to be essential for early embryogene ...
Essential Question
Essential Question

... What is the complimentary sequence: A A T G C A TTA CGT 8. The two sides of DNA are held together by Hydrogen _______ bonds. 9. DNA is composed of repeating subunits called nucleotides ______________________ 10. What are the 4 bases that make up the rungs of the DNA Adenine Thymine Guanine Cytosine ...
Chemistry 5.50 Site Directed Mutagenesis Methods. Site directed
Chemistry 5.50 Site Directed Mutagenesis Methods. Site directed

... methods are described below. All of these methods are now available in "kit" form were the details of the biology are described. A generic overview of the method is described in Figure 1. This figure was redrawn based on the figure from Cosby and Lesley (1997) Promega Notes Magazine Number 61, 12. I ...
DNA Bases
DNA Bases

... • DNA replication is the process of copying DNA • It occurs during S-phase of the cell cycle • Makes 2 exact copies of a cells DNA ...
File
File

... A.) Introns are the parts of mRNA that are translated. B.) Introns are removed during translation C.) In general, human genes have fewer introns than genes of other organisms. ____35.) Which mode of information transfer usually does not occur? A.) DNA to DNA B.) DNA to RNA C.) DNA to protein D.) All ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... A male displays the characteristics of Klinefelter syndrome (XXY). Which of his parents underwent nondisjunction during meiosis, giving rise to the gamete responsible for the ...
DNA is like blueprints, instructions, or a code for making proteins
DNA is like blueprints, instructions, or a code for making proteins

... that consists of a long chain of nucleotide units. ...
Quiz-3
Quiz-3

... hairs and blood samples were collected from the crime seen. There are two suspects in custody. Write down step by step instruction to identify the guilty. 28. Suppose the DNA fingerprints of the samples did not match with any suspects but they matched with the victim. What will be your guess about t ...
MCB Lecture 9 – Mitchondria – Van Oost
MCB Lecture 9 – Mitchondria – Van Oost

... o Men NEVER pass down the disease. What are the three major types of mutations in Mitochondrial DNA? o Rearrangements that generate deletions o Point mutations o Missense mutations When mutations happen in Mitochondrial DNA, what types of tissues are affected first? What do those tissues do as a res ...
Chapter 11 Jeopardy Review
Chapter 11 Jeopardy Review

... •tRNA attaches to amino acids from the cytoplasm and brings them to the ribosome •DNA replicates itself in the nucleus •The single, unzipped DNA strand is transcribed onto the mRNA •DNA unzips •tRNA anticodons bind with the codons on the ribosome ...
Module 16 - MDC Faculty Web Pages
Module 16 - MDC Faculty Web Pages

... 3 the transfer of genetic information from DNA by the formation of mRNA 4 the combination of a pentose sugar and a nitrogen-containing base 5 nitrogen-containing base found in both DNA and RNA (derived from purine and pairs with cytosine) 7 the process of duplicating DNA by pairing the bases on each ...
dna review - NVHSIntroBioPiper1
dna review - NVHSIntroBioPiper1

... are tall (T) and some that are short (t). The genotypes of the parents are probably… A. TT and TT B. Tt and Tt C. TT and tt D. TT and Tt 2. If all of the sons of a normal male have hemophilia, a sex-linked disorder, and all of their daughters are carriers, what must be true ...
DNA Introduction Questions
DNA Introduction Questions

... What are 2 main functions of DNA? (see p.40) ...
DNA Paternity Test RFLP analysis (Restriction Fragment Length
DNA Paternity Test RFLP analysis (Restriction Fragment Length

... -due to spontaneous mutations over time, different people have slightly different base sequences in their DNA -if mutation creates or deletes a restriction site in the DNA, the new DNA will generate more or less fragments/different sized fragments when cut with a particular enzyme ...
DNA Replication and recombination
DNA Replication and recombination

... exam 1, a bit shorter. You will need a bluebook again. 2. Answers from exam 1 will be removed from lab when exam 2 answers go up. 3. Homework/ problem set 4 (15 pts) due next week in lab. ...
DNA
DNA

... The bases are held together by a weak hydrogen bond. Two (2) bonds between A and T, three (3) bonds between C and G. ...
NAME: CLASS:______ DNA - The Double Helix Recall that the
NAME: CLASS:______ DNA - The Double Helix Recall that the

... DNA - The Double Helix Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. How does it do this? The nucleus controls these activities by the chromosom ...
Chromosomal Structure HWK
Chromosomal Structure HWK

... (b) A telomere is a long sequence of repetitive, noncoding DNA that is found at the end of chromosomes, while a centromere is a constricted region of a chromosome that holds two replicated chromosome strands together (c) A LINE is a DNA sequence of 5000 to 7000 nucleotides that are repetitive and al ...
The-Human-Genome
The-Human-Genome

... require the destruction of a DNA record or sample after a conviction has been overturned. There is a chance that a persons entire genome may be available regardless of whether they were convicted or not.(1) Arrestees are often found innocent of a particular committed crime, the retention of innocent ...
Section 18.2
Section 18.2

... • You can think of catalysts as helper molecules that allow a reaction to proceed in many small steps instead of all at once. ...
Name: _________Date: : _____ Assignment #_____ Chapter 12
Name: _________Date: : _____ Assignment #_____ Chapter 12

... 12-2: Chromosomes and DNA Replication 1. Where is DNA found in prokaryotic cells? ______________________ 2. DNA wraps around proteins called _______________________ to form _________________________. 3. At the beginning of cell division, the DNA and proteins pack together even tighter to form indivi ...
Genetics Study Guide
Genetics Study Guide

... Gregor Mendel found through his experiments that alleles can be _____________ or _______________ . DNA is condensed into structures called ____________________________ . Which nitrogen base is in RNA but not in DNA? _______________________ Deoxyribose in DNA is a _________________ . DNA can be desc ...
Directions for Dog Breed Genetics
Directions for Dog Breed Genetics

... how DNA is transcribed into mRNA which is then translated into an amino acid sequence that makes up a specific protein. The domesticated dog (Canis familiaris) is an interesting species to study because it is possibly the most diverse mammal species on Earth. There are more than 300 dog breeds that ...
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DNA profiling



DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.
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