RC 2 Student Notes
... DNA has a 1:1 ratio of pyrimidine and purine bases; the amount of -Guanine = Cytosine, Adenine = Thymine ...
... DNA has a 1:1 ratio of pyrimidine and purine bases; the amount of -Guanine = Cytosine, Adenine = Thymine ...
Chapter 12
... To find what molecule caused transformations they treated the mixtures w/ enzymes that killed proteins, lipids, carbohydrates, RNA, and then DNA. -Occured in all except one w/ DNA killed Avery and his team discovered that DNA stores and transmits genetic info. from generation to generation ...
... To find what molecule caused transformations they treated the mixtures w/ enzymes that killed proteins, lipids, carbohydrates, RNA, and then DNA. -Occured in all except one w/ DNA killed Avery and his team discovered that DNA stores and transmits genetic info. from generation to generation ...
4-14
... Subject: Gene mutation. Reading in ‘An introduction to genetic analysis’ (Griffiths et al., 7th edition) Chapter 15: Gene mutation ________________________________________________________________________ Key concepts: How DNA changes affect phenotype (15-1, 15-2) ...
... Subject: Gene mutation. Reading in ‘An introduction to genetic analysis’ (Griffiths et al., 7th edition) Chapter 15: Gene mutation ________________________________________________________________________ Key concepts: How DNA changes affect phenotype (15-1, 15-2) ...
the Genes?
... -- About the same as a plant (Arabidopsis, 25,000) -- Two groups estimate 30,000 to 35,000, but there is only partial overlap in their gene lists! -- One Drosophila gene potentially yields 38,000 distinct proteins by alternative splicing. -- A microarray-based survey of chromosomes 21, 22 finds 10 t ...
... -- About the same as a plant (Arabidopsis, 25,000) -- Two groups estimate 30,000 to 35,000, but there is only partial overlap in their gene lists! -- One Drosophila gene potentially yields 38,000 distinct proteins by alternative splicing. -- A microarray-based survey of chromosomes 21, 22 finds 10 t ...
PCR reading answers
... 12. Briefly explain the role of each enzyme for in vivo replication....... topoisomerase - stabilizes the DNA helix ahead of the replication fork ; it does allow for some unwinding of the double helix in a controlled manner during replication or transcription.... .....literal translation = "enzyme ...
... 12. Briefly explain the role of each enzyme for in vivo replication....... topoisomerase - stabilizes the DNA helix ahead of the replication fork ; it does allow for some unwinding of the double helix in a controlled manner during replication or transcription.... .....literal translation = "enzyme ...
20.1 Structural Genomics Determines the DNA Sequences of Entire
... • Whole-genome shotgun sequencing: Small-insert clones are prepared directly from genomic DNA and sequenced in a highly automated way. ...
... • Whole-genome shotgun sequencing: Small-insert clones are prepared directly from genomic DNA and sequenced in a highly automated way. ...
Zebra fish
... mutagenic viral insertion and a viral-specific primer were used in a single reaction • A viral insertion leads to amplification between the viralspecific primer and one of the genomic primers ...
... mutagenic viral insertion and a viral-specific primer were used in a single reaction • A viral insertion leads to amplification between the viralspecific primer and one of the genomic primers ...
Slide 1
... What is the role of these primers? Why are two different primers required? When DNA strands are separated in the PCR, what type of bond is broken? It is important in the PCR that the fragments of DNA used are not contaminated with any other biological material. Suggest a reason why. ...
... What is the role of these primers? Why are two different primers required? When DNA strands are separated in the PCR, what type of bond is broken? It is important in the PCR that the fragments of DNA used are not contaminated with any other biological material. Suggest a reason why. ...
After Cell parts, Mitosis Test, and Cell Energy Test: Put following in
... 4. A mutation may result in a protein that does not work correctly, resulting in ________________________ or _____________________________ problems in cells and organisms. If a protein is nonfunctional, the embryo may _______________________________________. 5. In rare cases mutations may __________ ...
... 4. A mutation may result in a protein that does not work correctly, resulting in ________________________ or _____________________________ problems in cells and organisms. If a protein is nonfunctional, the embryo may _______________________________________. 5. In rare cases mutations may __________ ...
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR
... most common allele that contains 18 and 24 replicates, while the rarest contains 14 and 38. There is no known phenotype associated with the D1S80 locus, making it ideal to distinguish people only by their DNA sequence. Polyacrylamide gel electrophoresis is the method of choice for analysis of the D1 ...
... most common allele that contains 18 and 24 replicates, while the rarest contains 14 and 38. There is no known phenotype associated with the D1S80 locus, making it ideal to distinguish people only by their DNA sequence. Polyacrylamide gel electrophoresis is the method of choice for analysis of the D1 ...
BASIC DNA
... • DNA Polymorphism (“many forms”) – Regions of DNA which differ from person to person • Locus (plural = loci) – Site or location on a chromosome • Allele – Different variants which can exist at a locus • DNA Profile – The combination of alleles for an individual ...
... • DNA Polymorphism (“many forms”) – Regions of DNA which differ from person to person • Locus (plural = loci) – Site or location on a chromosome • Allele – Different variants which can exist at a locus • DNA Profile – The combination of alleles for an individual ...
Topic 6 - DNA, RNA, Protein Synthesis
... This process occurs in the cytoplasm. In the process of translation, the piece of mRNA is read by the ribosome in groups of three letters (codons). Each 3-letter portion of mRNA is referred to as a codon and codes for a specific amino acid. These codes match up to the anticodons on the bottom of the ...
... This process occurs in the cytoplasm. In the process of translation, the piece of mRNA is read by the ribosome in groups of three letters (codons). Each 3-letter portion of mRNA is referred to as a codon and codes for a specific amino acid. These codes match up to the anticodons on the bottom of the ...
DNA
... • Genes are DNA –encoded information that specifies particular proteins; each gene is made of a specific sequence of nucleotides. • Genes are composed of coding and noncoding sequences. – Coding sequences are exons (code for amino acids) – Noncoding sequences are introns (intervening) ...
... • Genes are DNA –encoded information that specifies particular proteins; each gene is made of a specific sequence of nucleotides. • Genes are composed of coding and noncoding sequences. – Coding sequences are exons (code for amino acids) – Noncoding sequences are introns (intervening) ...
BioDynami 1 kb plus DNA ladder, ready-to-load
... For longer periods, store at -20°C. Description For sizing and quantification of double strand DNA fragments. Composed of 13 bands as shown on right. The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others. Premixed with 6X DNA loading buffer for direct ge ...
... For longer periods, store at -20°C. Description For sizing and quantification of double strand DNA fragments. Composed of 13 bands as shown on right. The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others. Premixed with 6X DNA loading buffer for direct ge ...
DNA structure
... Phage viruses only have protein & DNA; tested to see which is injected into host Concludes DNA is the genetic material. ...
... Phage viruses only have protein & DNA; tested to see which is injected into host Concludes DNA is the genetic material. ...
CHAPTER 8 MICROBIAL GENETICS What is genetics? Terminology
... • Segments of DNA that can move from one region of DNA to another • Contain insertion sequences for cutting and resealing DNA (transposase) • Complex transposons carry other genes ...
... • Segments of DNA that can move from one region of DNA to another • Contain insertion sequences for cutting and resealing DNA (transposase) • Complex transposons carry other genes ...
Review Questions
... (We did this one in class) 1. Making a copy of DNA is called _________________________. 2. Which nitrogen base isn’t used during this process? 3. Name the enzyme you learned about that adds the complementary nucleotides and spell checks to make sure the new copy is correct. http://bio.usuhs.mil/bioc ...
... (We did this one in class) 1. Making a copy of DNA is called _________________________. 2. Which nitrogen base isn’t used during this process? 3. Name the enzyme you learned about that adds the complementary nucleotides and spell checks to make sure the new copy is correct. http://bio.usuhs.mil/bioc ...
Chromosomes
... Double-stranded supercoiled circular DNA molecule The length is 2 - 5×106 bp. 1 ori-site (one replicon). Attached to plasma membrane in the ori-site region. Associated with only a few protein molecules. Structural gene sequences (encoding proteins and RNAs) account for the majority of bacterial DNA ...
... Double-stranded supercoiled circular DNA molecule The length is 2 - 5×106 bp. 1 ori-site (one replicon). Attached to plasma membrane in the ori-site region. Associated with only a few protein molecules. Structural gene sequences (encoding proteins and RNAs) account for the majority of bacterial DNA ...
KUPSHO
... c. triple In DNA the base A (adenine) forms a bond with the base____. In RNA the base A (adenine) forms a bond with the base ____. In DNA the base C (Cytosine) forms a bond with the base ____. In DNA the bases A and T are held together by a ___ bond. a. single b. double c. triple In DNA the bases G ...
... c. triple In DNA the base A (adenine) forms a bond with the base____. In RNA the base A (adenine) forms a bond with the base ____. In DNA the base C (Cytosine) forms a bond with the base ____. In DNA the bases A and T are held together by a ___ bond. a. single b. double c. triple In DNA the bases G ...
Prescott`s Microbiology, 9th Edition Chapter 16 –Mechanisms of
... If non homologous DNA was taken into the cell, it would not be able to align itself with a homologous region on the bacterial chromosome, and thus would not be integrated. The DNA could be degraded, but if not, could still only be passed to one daughter cell during cell division, and would thus be d ...
... If non homologous DNA was taken into the cell, it would not be able to align itself with a homologous region on the bacterial chromosome, and thus would not be integrated. The DNA could be degraded, but if not, could still only be passed to one daughter cell during cell division, and would thus be d ...
Worksheet – Structure of DNA and Replication
... Directions: Complete each sentence. 7. Guanine, cytosine, thymine, and __________________ are the four __________________ in DNA. 8. In DNA, guanine always forms hydrogen bonds with __________________. 9. The process of __________________ produces a new copy of an organism’s genetic information, wh ...
... Directions: Complete each sentence. 7. Guanine, cytosine, thymine, and __________________ are the four __________________ in DNA. 8. In DNA, guanine always forms hydrogen bonds with __________________. 9. The process of __________________ produces a new copy of an organism’s genetic information, wh ...
Name
... 1. Isolate DNA of interest – cut desired gene with restriction enzymes and cut plasmid with same restriction enzyme 2. Allow gene of interest and plasmid to anneal (DNA ligase – joins the two pieces of DNA together) 3. Insert plasmid into bacterium 4. Allow bacterium to replicate (cloning) 5. Screen ...
... 1. Isolate DNA of interest – cut desired gene with restriction enzymes and cut plasmid with same restriction enzyme 2. Allow gene of interest and plasmid to anneal (DNA ligase – joins the two pieces of DNA together) 3. Insert plasmid into bacterium 4. Allow bacterium to replicate (cloning) 5. Screen ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.