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Review for Unit 7 Exam
Review for Unit 7 Exam

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國立彰化師範大學100 學年度碩士班招生考試試題

... b. Cut the plasmid DNA using restriction enzymes; c. Extract plasmid DNA from bacterial cells; d. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments; e. Use ligase to seal plasmid DNA to nonplasmid DNA. (A) acbde. (B) bceda. (C) cbdea. (D) dcbea. 29. Which of the following tools of recombinan ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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