University of Debrecen - DEA
... layer is not constructed solely of phospholipid and protein, like cytoplasmic membrane, but also contains polysaccharide. The lipid and polysaccharide are linked in the outer membrane to form a complex (lipopolysaccharide - LPS). The polysaccharide portion of LPS consists of two components, the core ...
... layer is not constructed solely of phospholipid and protein, like cytoplasmic membrane, but also contains polysaccharide. The lipid and polysaccharide are linked in the outer membrane to form a complex (lipopolysaccharide - LPS). The polysaccharide portion of LPS consists of two components, the core ...
Identification of a Novel Streptococcal Gene
... vive DNA damage by synthesizing through DNA lesions that block replication forks (63). In E. coli, almost all SOS-targeted UV mutagenesis results from the activity of PolV (53, 64), and the umuDC operon is the only SOS locus that must be induced for SOS mutagenesis (61). PolV consists of one molecul ...
... vive DNA damage by synthesizing through DNA lesions that block replication forks (63). In E. coli, almost all SOS-targeted UV mutagenesis results from the activity of PolV (53, 64), and the umuDC operon is the only SOS locus that must be induced for SOS mutagenesis (61). PolV consists of one molecul ...
Hydrogen autotrophy of Nocardia opaca strains is
... opaca studied. This success is also due to the application of an alternative method for preparation of DNA in agarose plugs containing the bacterial cells. For unknown reasons this method was found not to be suited to detecting the cccDNA plasmids of N. opaca. Only one among the three linear plasmid ...
... opaca studied. This success is also due to the application of an alternative method for preparation of DNA in agarose plugs containing the bacterial cells. For unknown reasons this method was found not to be suited to detecting the cccDNA plasmids of N. opaca. Only one among the three linear plasmid ...
Molecular Genetics
... Answer: A mutagen in a body cell becomes part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in eve ...
... Answer: A mutagen in a body cell becomes part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in eve ...
Slide 1
... Answer: A mutagen in a body cell becomes part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in eve ...
... Answer: A mutagen in a body cell becomes part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in eve ...
Solutions to Genetics Day 6 Interpretation Questions
... Any change that prevents transposase from being expressed, any change that interferes with phage adsorption, any change that allowed the λ phage to form a lysogene or lyse the cells could have prevented random insertion from occurring. How was the non-random insertion of DNA into the bacterial genom ...
... Any change that prevents transposase from being expressed, any change that interferes with phage adsorption, any change that allowed the λ phage to form a lysogene or lyse the cells could have prevented random insertion from occurring. How was the non-random insertion of DNA into the bacterial genom ...
Degenerate PCR - Yale School of Medicine
... Nusieve:SeaKem LE agarose (you can buy this premixed from FMC) in 1X TAE. This gel is not very low melting, and thus isn't very suitable for cloning directly from the gel, but it gives very nice resolution. I use the 123 bp ladder from Gibco as a size standard. Obviously you expect to get products o ...
... Nusieve:SeaKem LE agarose (you can buy this premixed from FMC) in 1X TAE. This gel is not very low melting, and thus isn't very suitable for cloning directly from the gel, but it gives very nice resolution. I use the 123 bp ladder from Gibco as a size standard. Obviously you expect to get products o ...
Full-Text PDF
... complex of DNA primase and DNA polymerase α (Polα) [1]. The primosome initiates synthesis of both the leading and lagging strands by making chimeric RNA-DNA primers, which are required for the loading of replication factor C (RFC), proliferating cell nuclear antigen (PCNA), and replicative DNA polym ...
... complex of DNA primase and DNA polymerase α (Polα) [1]. The primosome initiates synthesis of both the leading and lagging strands by making chimeric RNA-DNA primers, which are required for the loading of replication factor C (RFC), proliferating cell nuclear antigen (PCNA), and replicative DNA polym ...
From Genetics to DNA
... pull apart. In the laboratory, the strength of this interaction can be measured by finding the temperature required to break the hydrogen bonds, their melting temperature (also called Tm value). When all the base pairs in a DNA double helix melt, the strands separate and exist in solution as two ent ...
... pull apart. In the laboratory, the strength of this interaction can be measured by finding the temperature required to break the hydrogen bonds, their melting temperature (also called Tm value). When all the base pairs in a DNA double helix melt, the strands separate and exist in solution as two ent ...
Expansion of tandem repeats and oligomer
... A study of SSR from primates, emphasizing their abundance, length polymorphism, and overall tendency to expand in di erent sequence contexts, was reported by Jurka and Pethiyagoda [10]. The probability distribution functions for the length of special classes of repeats have been studied in many publ ...
... A study of SSR from primates, emphasizing their abundance, length polymorphism, and overall tendency to expand in di erent sequence contexts, was reported by Jurka and Pethiyagoda [10]. The probability distribution functions for the length of special classes of repeats have been studied in many publ ...
Nucleic Acid Structures, Energetics, and Dynamics
... strand. Four deoxynucleoside triphosphates (deoxy-NTPs) are the monomer units polymerized by the enzyme. Normally the enzyme will continue synthesis until it runs out of deoxy-NTPs or of template strands. However, if a few percent of a dideoxyNTP is added to the reaction mixture, the dideoxy-NTP act ...
... strand. Four deoxynucleoside triphosphates (deoxy-NTPs) are the monomer units polymerized by the enzyme. Normally the enzyme will continue synthesis until it runs out of deoxy-NTPs or of template strands. However, if a few percent of a dideoxyNTP is added to the reaction mixture, the dideoxy-NTP act ...
DNA Sequence Representation by Use of Statistical Finite Automata
... further depending upon their chemical property - polarity. Each amino acid has an amino group and a side chain which varies in polarity, depending on its structure. Some are hydrophobic being non-polar and the rest are hydrophilic being polar, which again can be subcategorized in neutral, acidic (n ...
... further depending upon their chemical property - polarity. Each amino acid has an amino group and a side chain which varies in polarity, depending on its structure. Some are hydrophobic being non-polar and the rest are hydrophilic being polar, which again can be subcategorized in neutral, acidic (n ...
Function of E. coli RNA Polymerase Factor 70 in
... since a few sequence changes in the early transcribed segment, particularly just after the final T, can convert a 210 consensus-induced pause at 125 of phage 82 to an arrested complex (Yarnell and Roberts, unpublished data). Second, there is a background of short transcripts extending to about 118 t ...
... since a few sequence changes in the early transcribed segment, particularly just after the final T, can convert a 210 consensus-induced pause at 125 of phage 82 to an arrested complex (Yarnell and Roberts, unpublished data). Second, there is a background of short transcripts extending to about 118 t ...
Rapid and specific determination of DNA adducts for clinical diagnosis of
... DNA adducts induced by AAs have been proven to be critical biomarkers for AA-associated diseases. Metabolic activation of AAs produces reactive aristolactam-nitrenium ion intermediates. Electrophilic attack of the aristolactamnitrenium ion via its C7 position on the exocyclic amino group in the puri ...
... DNA adducts induced by AAs have been proven to be critical biomarkers for AA-associated diseases. Metabolic activation of AAs produces reactive aristolactam-nitrenium ion intermediates. Electrophilic attack of the aristolactamnitrenium ion via its C7 position on the exocyclic amino group in the puri ...
Molecular Structure of the GARP Family of Plant Myb
... vitro. These results are essentially consistent with the recognition sequences proposed for ARR10-RB (Figure 4) and show that the isolated single B motif is capable of binding to DNA in a sequence-specific manner. To quantitate the DNA binding ability of ARR10-B, the binding affinity of the protein ...
... vitro. These results are essentially consistent with the recognition sequences proposed for ARR10-RB (Figure 4) and show that the isolated single B motif is capable of binding to DNA in a sequence-specific manner. To quantitate the DNA binding ability of ARR10-B, the binding affinity of the protein ...
DNA and RNA extraction
... Centrifuge at 12,000 x g for 2 minutes. This will precipitate the carbohydrate. Transfer the supernatant to a fresh tube for precipitation of the RNA. High-molecular weight RNA (including mRNA) is recovered by selective precipitation with NaCl. At 2.5M NaCl, DNA and low molecular weight RNA remain i ...
... Centrifuge at 12,000 x g for 2 minutes. This will precipitate the carbohydrate. Transfer the supernatant to a fresh tube for precipitation of the RNA. High-molecular weight RNA (including mRNA) is recovered by selective precipitation with NaCl. At 2.5M NaCl, DNA and low molecular weight RNA remain i ...
High Frequency of Recombination (Hfr)
... Hfr DNA that is not incorporated in the F- strand, and DNA that has crossed out of the F- strand is ...
... Hfr DNA that is not incorporated in the F- strand, and DNA that has crossed out of the F- strand is ...
A Sequential Strand-Displacement Strategy Enables Efficient Six
... ability of a single-stranded DNA oligonucleotide (AB) to invade an asymmetric DNA duplex (A0 B0 :B) that contains a single-stranded hybridization site (A0 ) for the invading strand.39 Once A:A0 hybridization takes place, base pairing with the invading strand continues, ultimately resulting in strand ...
... ability of a single-stranded DNA oligonucleotide (AB) to invade an asymmetric DNA duplex (A0 B0 :B) that contains a single-stranded hybridization site (A0 ) for the invading strand.39 Once A:A0 hybridization takes place, base pairing with the invading strand continues, ultimately resulting in strand ...
High Frequency of Recombination (Hfr)
... Hfr DNA that is not incorporated in the F- strand, and DNA that has crossed out of the F- strand is ...
... Hfr DNA that is not incorporated in the F- strand, and DNA that has crossed out of the F- strand is ...
Huntingtin grabs a hammer: DNA repair in HD
... huntingtin might enter the nucleus in response to stressors that can cause single-base DNA lesions. These lesions affect only one “letter” of a single pair in the genetic code, ...
... huntingtin might enter the nucleus in response to stressors that can cause single-base DNA lesions. These lesions affect only one “letter” of a single pair in the genetic code, ...
RNA and Protein Synthesis
... DNA contains bases adenine, thymine, guanine, cytosine. DNA never leaves the nucleus. ...
... DNA contains bases adenine, thymine, guanine, cytosine. DNA never leaves the nucleus. ...
Lesson Plan
... 3. The DNA is organized into tightly coiled strands known as chromosomes. 4. Segments of the chromosomes that code for specific traits of the organism are genes. B. The genetic material in the cell determines the appearance of an organism and all chemical processes. 1. Genes that govern variations o ...
... 3. The DNA is organized into tightly coiled strands known as chromosomes. 4. Segments of the chromosomes that code for specific traits of the organism are genes. B. The genetic material in the cell determines the appearance of an organism and all chemical processes. 1. Genes that govern variations o ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.