Supercoils in plant DNA: nucleoid
... have been interpreted as an indication that eukaryotic DNA is supercoiled. Interestingly, similar observations have been reported on the so-called folded Dmsophila genomes prepared in 0-9M-NaCl, i.e. on nuclear structures lacking only histone HI but preserving the rest of the histones (Benyajati & W ...
... have been interpreted as an indication that eukaryotic DNA is supercoiled. Interestingly, similar observations have been reported on the so-called folded Dmsophila genomes prepared in 0-9M-NaCl, i.e. on nuclear structures lacking only histone HI but preserving the rest of the histones (Benyajati & W ...
Enhancing fairness in DNA jury trials
... 23 times more likely to vote guilty in homicide cases and 33 times more likely to vote guilty in sexual assault cases when DNA evidence was admitted (Briody 2004). Concern has arisen that the safety of these verdicts may be compromised by widespread misconceptions about the infallibility of DNA evid ...
... 23 times more likely to vote guilty in homicide cases and 33 times more likely to vote guilty in sexual assault cases when DNA evidence was admitted (Briody 2004). Concern has arisen that the safety of these verdicts may be compromised by widespread misconceptions about the infallibility of DNA evid ...
Evolutionary conservation and versatility of a new set of primers for
... initial denaturation at 94 °C for 4 min, 35 cycles were performed, each consisting of melting at 95 °C for 20 s, annealing at the appropriate temperatures (Table 1) for 40 s and extension at 72 °C for 20 s. A final extension was run at 72 °C for 2 min. A volume (5 µL) of the reaction was then examin ...
... initial denaturation at 94 °C for 4 min, 35 cycles were performed, each consisting of melting at 95 °C for 20 s, annealing at the appropriate temperatures (Table 1) for 40 s and extension at 72 °C for 20 s. A final extension was run at 72 °C for 2 min. A volume (5 µL) of the reaction was then examin ...
Article - School of Chemistry and Biochemistry
... The α/β core is similar to that of BamHI, containing a six-stranded β-sheet (β1, β3, β4, β5, β6 and β7) surrounded by five α-helices (α1, α2, α3, α4 and α5), two of which (α4 and α5) are involved in homodimerization. The loops preceding the dimerization helices (loops B and C) carry the residues tha ...
... The α/β core is similar to that of BamHI, containing a six-stranded β-sheet (β1, β3, β4, β5, β6 and β7) surrounded by five α-helices (α1, α2, α3, α4 and α5), two of which (α4 and α5) are involved in homodimerization. The loops preceding the dimerization helices (loops B and C) carry the residues tha ...
DNA denaturation in the rodlike polyelectrolyte model
... electrostatic interaction. As result, the balancing between salt concentration and the melting temperature (Tm ) of duplex DNA is being proposed as the mechanism for controlling the denaturation process [31–34]. A number of models have been focused in the local process of the denaturation as the res ...
... electrostatic interaction. As result, the balancing between salt concentration and the melting temperature (Tm ) of duplex DNA is being proposed as the mechanism for controlling the denaturation process [31–34]. A number of models have been focused in the local process of the denaturation as the res ...
"An In Vitro Selection Protocol for Threose Nucleic Acid (TNA) Using
... Threose nucleic acid (TNA) is an unnatural genetic polymer composed of repeating threofuranosyl sugars linked by 2 and 3 phosphodiester bonds. TNA is capable of forming antiparallel Watson-Crick duplex structures in a self-pairing mode, and can also cross-pair opposite complementary strands of DNA ...
... Threose nucleic acid (TNA) is an unnatural genetic polymer composed of repeating threofuranosyl sugars linked by 2 and 3 phosphodiester bonds. TNA is capable of forming antiparallel Watson-Crick duplex structures in a self-pairing mode, and can also cross-pair opposite complementary strands of DNA ...
Bitter-Tasting Ability
... (RFLP) that can be separated on a 2% agarose gel. Each student scores his or her genotype, predicts their tasting ability, and ...
... (RFLP) that can be separated on a 2% agarose gel. Each student scores his or her genotype, predicts their tasting ability, and ...
1st set of Journal Clubs this Wednesday!
... Monod showed that Z+Y- mutants did not take up a radioactively labeled galactoside in the presence of inducer. He concluded that lacY must encode an enzyme responsible for transporting the galactoside into cells: called it galactoside permease. ...
... Monod showed that Z+Y- mutants did not take up a radioactively labeled galactoside in the presence of inducer. He concluded that lacY must encode an enzyme responsible for transporting the galactoside into cells: called it galactoside permease. ...
Optimization of the RT-PCR Method Using the TitanTM One Tube
... In this method both the cDNA synthesis and the amplification are performed with an optimized buffer and the respective enzyme one after the other, but without any more addition of reagents. A distinction is made between two approaches: a. The use of T. thermophilus-(Tth-)DNA polymerase which, like r ...
... In this method both the cDNA synthesis and the amplification are performed with an optimized buffer and the respective enzyme one after the other, but without any more addition of reagents. A distinction is made between two approaches: a. The use of T. thermophilus-(Tth-)DNA polymerase which, like r ...
LP - Columbia University
... Sister/Sibling Chromatids: The DNA molecules in sister chromatids are identical because they are the two products of a single semi-conservative DNA replication. How many chromatids per chromosome? Can be 1 or 2; depends on where cell is in the cell cycle. Before S, each chromosome has one chromatid ...
... Sister/Sibling Chromatids: The DNA molecules in sister chromatids are identical because they are the two products of a single semi-conservative DNA replication. How many chromatids per chromosome? Can be 1 or 2; depends on where cell is in the cell cycle. Before S, each chromosome has one chromatid ...
Chapter 9 - People Server at UNCW
... • Used E.coli bacteria infected with a virus that consisted of a protein head surrounding DNA • Grew a batch of virus in a medium containing 35S and 32P • Blender experiments showed that the virus transfers DNA, not protein, into a bacterial cell • Thus, DNA is the genetic material ...
... • Used E.coli bacteria infected with a virus that consisted of a protein head surrounding DNA • Grew a batch of virus in a medium containing 35S and 32P • Blender experiments showed that the virus transfers DNA, not protein, into a bacterial cell • Thus, DNA is the genetic material ...
Histones and histone-modifying enzymes
... species, but these genes are more closely related to those identified in B. petri (Figure ...
... species, but these genes are more closely related to those identified in B. petri (Figure ...
Lessons 9
... strung together that wrap around each other. It is often compared to a ladder when it is unwound with the sugar-phosphate backbone acting as the rails of the ladder while the nitrogenous bases from both strands, face one another like the ladder rungs. As first evidenced in Franklin’s X-ray picture, ...
... strung together that wrap around each other. It is often compared to a ladder when it is unwound with the sugar-phosphate backbone acting as the rails of the ladder while the nitrogenous bases from both strands, face one another like the ladder rungs. As first evidenced in Franklin’s X-ray picture, ...
Final Research Genetics
... stopping of DNA synthesis by Si starvation is assumed to be caused by a decrease in activity of the enzymes DNA polymerase(s), not because of a lack of energy or precursors, (Pan, 1998). The decrease of DNA synthesis as a result of Si limitation may arrest cells at a particular phase in the cell div ...
... stopping of DNA synthesis by Si starvation is assumed to be caused by a decrease in activity of the enzymes DNA polymerase(s), not because of a lack of energy or precursors, (Pan, 1998). The decrease of DNA synthesis as a result of Si limitation may arrest cells at a particular phase in the cell div ...
Methylation-sensitive RFLPs: characterisation of two oil palm
... whole genome. High-performance liquid chromatography (HPLC) estimation of genomic 5-methyl-deoxycytosine (5mdC) amounts demonstrated the occurrence of a significant hypomethylation in FGC (–4.5%; P<10–5) and leaves from “mantled” regenerants (–1.2%; P<10–5), compared with their normal counterparts. ...
... whole genome. High-performance liquid chromatography (HPLC) estimation of genomic 5-methyl-deoxycytosine (5mdC) amounts demonstrated the occurrence of a significant hypomethylation in FGC (–4.5%; P<10–5) and leaves from “mantled” regenerants (–1.2%; P<10–5), compared with their normal counterparts. ...
Things to know for the Final - Mercer Island School District
... DNA Replication and Gene Expression: Transcription and Translation (Chapter 8) Understand the structure of a DNA molecule, including knowing the 3 parts of the nucleotide subunits of DNA, and how the nucleotides are arranged in a DNA molecule. Be able to compare and contrast DNA and RNA (include 3 d ...
... DNA Replication and Gene Expression: Transcription and Translation (Chapter 8) Understand the structure of a DNA molecule, including knowing the 3 parts of the nucleotide subunits of DNA, and how the nucleotides are arranged in a DNA molecule. Be able to compare and contrast DNA and RNA (include 3 d ...
Biology Ch. 12
... Answer: A mutagen in a body cell becomes part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in eve ...
... Answer: A mutagen in a body cell becomes part of the of the genetic sequence in that cell and in future daughter cells. The cell may die or simply not perform its normal function. These mutations are not passed on to the next generation. When mutations occur in sex cells, they will be present in eve ...
U6 snRNA genes of Arabidopsis are transcribed
... and 7SK (17,18) RNAs in vertebrates are transcribed by pol HI but do not require any intragenic elements. Instead, their activity depends upon three upstream signals: the Distal and Proximal Sequence Elements (DSE and PSE), and an AT-rich box which resembles the TATA box found in mRNA genes. The fir ...
... and 7SK (17,18) RNAs in vertebrates are transcribed by pol HI but do not require any intragenic elements. Instead, their activity depends upon three upstream signals: the Distal and Proximal Sequence Elements (DSE and PSE), and an AT-rich box which resembles the TATA box found in mRNA genes. The fir ...
DNA Electrophoresis of precut restriction digests – the WHODUNNIT
... From your standard curve for HindIII, made from known fragment sizes, you can calculate fragment sizes resulting from a digest with EcoRI. The procedure is as follows. 1. Measure the migration distance in mm for each EcoRI band. Record the data in the table under measured distance. 2. Determine the ...
... From your standard curve for HindIII, made from known fragment sizes, you can calculate fragment sizes resulting from a digest with EcoRI. The procedure is as follows. 1. Measure the migration distance in mm for each EcoRI band. Record the data in the table under measured distance. 2. Determine the ...
Lab 1 genomic DNA
... Two common reducing agents found in extraction buffers are ß-mercaptoethanol or dithiothreitol (DTT). EDTA is also present to chelate Mg+2 ions thus mediating aggregation of nucleic acids to each other and to proteins. Ethanol and/or Isopropanol Precipitation ...
... Two common reducing agents found in extraction buffers are ß-mercaptoethanol or dithiothreitol (DTT). EDTA is also present to chelate Mg+2 ions thus mediating aggregation of nucleic acids to each other and to proteins. Ethanol and/or Isopropanol Precipitation ...
The Recombinant DNA Controversy: A Contemporary
... or gene cloner does. The million sheets of paper cannot be confused with a million cars. The many copies of a gene generated through recombinant DNA technology stand in precisely the same relation to the organism from which the gene was isolated as does the stack of paper to the car. Indeed the anal ...
... or gene cloner does. The million sheets of paper cannot be confused with a million cars. The many copies of a gene generated through recombinant DNA technology stand in precisely the same relation to the organism from which the gene was isolated as does the stack of paper to the car. Indeed the anal ...
Collaborative coupling between polymerase and helicase for
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
PDF
... To allow analysis of the nucleocytoplasmic architecture at high magnification, we decided to use sections through epon-embedded material. This approach has the advantage that well-fixed embryos can be sectioned at a precise thickness, normally 2-3 /MR, but thinner sections of large areas can also be ...
... To allow analysis of the nucleocytoplasmic architecture at high magnification, we decided to use sections through epon-embedded material. This approach has the advantage that well-fixed embryos can be sectioned at a precise thickness, normally 2-3 /MR, but thinner sections of large areas can also be ...
On the feasibility of using network processors for DNA processing
... a molecular biologist to search nucleotide and protein databases with a specific query [2]. For illustration purposes, we assume in this paper that all relevant information consists of DNA nucleotides (of which there exist exactly four, denoted by ‘A’, ‘C’, ‘G’ and ‘T’ respectively). Nevertheless, t ...
... a molecular biologist to search nucleotide and protein databases with a specific query [2]. For illustration purposes, we assume in this paper that all relevant information consists of DNA nucleotides (of which there exist exactly four, denoted by ‘A’, ‘C’, ‘G’ and ‘T’ respectively). Nevertheless, t ...
DNA
... Objective: Describe the structure for units of DNA. ___DNA___ is a _polymer_ made of _repeating_ subunits called _nucleotides_ (the monomer). _Nucleotides_ have three parts: a simple __sugar__, a _phosphate_ __group__, and a _nitrogenous_ ___base___. In DNA there are ___four___ possible _nucle ...
... Objective: Describe the structure for units of DNA. ___DNA___ is a _polymer_ made of _repeating_ subunits called _nucleotides_ (the monomer). _Nucleotides_ have three parts: a simple __sugar__, a _phosphate_ __group__, and a _nitrogenous_ ___base___. In DNA there are ___four___ possible _nucle ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.