Cleavage of a model DNA replication fork by a Type I restriction
... When a replication fork meets damaged DNA, it will be stalled. This leads to replication restart by error-prone polymerase-mediated translesion synthesis or by errorfree homologous recombination (1–3). Multiple models have been proposed for the latter pathway, most of which envision restart without ...
... When a replication fork meets damaged DNA, it will be stalled. This leads to replication restart by error-prone polymerase-mediated translesion synthesis or by errorfree homologous recombination (1–3). Multiple models have been proposed for the latter pathway, most of which envision restart without ...
Recombinational Circularization of Salmonella Phage
... cannula. In some cases, fractions were collected onto paper strips, which were dried and counted in a toluene-based scintillation fluid (Smith, ...
... cannula. In some cases, fractions were collected onto paper strips, which were dried and counted in a toluene-based scintillation fluid (Smith, ...
CHAPTER 7 DNA Mutation, DNA Repair and Transposable Elements
... i. Different his tester strains are available, to test for base-substitution and frameshift mutations. ii. Liver enzymes (the S9 extract) are mixed with the test chemical to determine whether the liver’s detoxification pathways convert it to a mutagenic form. iii. More revertants in the region of th ...
... i. Different his tester strains are available, to test for base-substitution and frameshift mutations. ii. Liver enzymes (the S9 extract) are mixed with the test chemical to determine whether the liver’s detoxification pathways convert it to a mutagenic form. iii. More revertants in the region of th ...
Lesson Overview
... In prokaryotes, RNA synthesis and protein synthesis take place in the cytoplasm. In eukaryotes, RNA is produced in the cell’s nucleus and then moves to the cytoplasm to play a role in the production of proteins. Our focus will be on transcription in eukaryotic cells. ...
... In prokaryotes, RNA synthesis and protein synthesis take place in the cytoplasm. In eukaryotes, RNA is produced in the cell’s nucleus and then moves to the cytoplasm to play a role in the production of proteins. Our focus will be on transcription in eukaryotic cells. ...
Chpt11_TxnPromoters.doc
... most efficient if the 5' phosphate is removed (by alkaline phosphatase) prior to the kinase treatment. b. 3' end label: Klenow DNA polymerase plus [ 32P] dNTP. The labeled dNTP is chosen to be complementary to the first position past the primer. A restriction fragment with a 5' overhang is ideal fo ...
... most efficient if the 5' phosphate is removed (by alkaline phosphatase) prior to the kinase treatment. b. 3' end label: Klenow DNA polymerase plus [ 32P] dNTP. The labeled dNTP is chosen to be complementary to the first position past the primer. A restriction fragment with a 5' overhang is ideal fo ...
Implications of DNA replication for eukaryotic gene expression
... transcriptional activity through cell division. This reflects the commitment of that cell type to a particular state of determination. How this commitment is established and maintained is not yet resolved; however, several recent experiments reviewed in this article suggest a solution to this proble ...
... transcriptional activity through cell division. This reflects the commitment of that cell type to a particular state of determination. How this commitment is established and maintained is not yet resolved; however, several recent experiments reviewed in this article suggest a solution to this proble ...
Text S2.
... ORF in the Gibson reaction. Primers WSCp10062 and WSCp10063 include 25bp sequences homologous to those flanking the unique NheI site in pTEV5. NheI-linearized pTEV5 and each divL PCR product were combined in equimolar amounts and incubated at 50C for 60 min in a reaction mixture containing T5 exonu ...
... ORF in the Gibson reaction. Primers WSCp10062 and WSCp10063 include 25bp sequences homologous to those flanking the unique NheI site in pTEV5. NheI-linearized pTEV5 and each divL PCR product were combined in equimolar amounts and incubated at 50C for 60 min in a reaction mixture containing T5 exonu ...
Volume 13 Number 3 A review of DNA repair and possible
... ends juxtaposed. DSBs can result from exposure to ionizing radiation, radiomimetic drugs and topoisomerase inhibitors. The main cellular response to DSBs include cell cycle regulation, DSB repair, transcriptional and post-transcriptional activation of relevant genes (including those associated with ...
... ends juxtaposed. DSBs can result from exposure to ionizing radiation, radiomimetic drugs and topoisomerase inhibitors. The main cellular response to DSBs include cell cycle regulation, DSB repair, transcriptional and post-transcriptional activation of relevant genes (including those associated with ...
Next-Generation DNA Sequencing Methods
... each incorporation of a nucleotide by DNA polymerase results in the release of pyrophosphate, which initiates a series of downstream reactions that ultimately produce light by the firefly enzyme luciferase. The amount of light produced is proportional to the number of nucleotides incorporated (up to t ...
... each incorporation of a nucleotide by DNA polymerase results in the release of pyrophosphate, which initiates a series of downstream reactions that ultimately produce light by the firefly enzyme luciferase. The amount of light produced is proportional to the number of nucleotides incorporated (up to t ...
from Saccharomyces cerevisiae
... to serve as templates for RNA synthesis. The alteration in DNA structure itself, or the subsequent release of specific trans-acting factors could have been the causative factor. It was important to demonstrate that at least a portion ofcentromere proteins remain associated after restriction digestio ...
... to serve as templates for RNA synthesis. The alteration in DNA structure itself, or the subsequent release of specific trans-acting factors could have been the causative factor. It was important to demonstrate that at least a portion ofcentromere proteins remain associated after restriction digestio ...
Transcription in Eukaryotes
... eukaryotic protein-coding gene. • Key experiment by Pierre Chambon and colleagues demonstrated that a viral TATA box is both necessary and sufficient for specific initiation of transcription by RNA pol II in vitro. ...
... eukaryotic protein-coding gene. • Key experiment by Pierre Chambon and colleagues demonstrated that a viral TATA box is both necessary and sufficient for specific initiation of transcription by RNA pol II in vitro. ...
(CH11) Transcription In Eukaryotes (Slides)
... eukaryotic protein-coding gene. • Key experiment by Pierre Chambon and colleagues demonstrated that a viral TATA box is both necessary and sufficient for specific initiation of transcription by RNA pol II in vitro. ...
... eukaryotic protein-coding gene. • Key experiment by Pierre Chambon and colleagues demonstrated that a viral TATA box is both necessary and sufficient for specific initiation of transcription by RNA pol II in vitro. ...
as Adobe PDF - Edinburgh Research Explorer
... 1993; Li and Botchan, 1993). On the basis of these observations, a model has been proposed where p53 binds to cellular origins of replication and there may interact with key replication proteins to block entry into S phase or to direct S phase cells into apoptosis (Pietenpol and Vogelstein, 1993). I ...
... 1993; Li and Botchan, 1993). On the basis of these observations, a model has been proposed where p53 binds to cellular origins of replication and there may interact with key replication proteins to block entry into S phase or to direct S phase cells into apoptosis (Pietenpol and Vogelstein, 1993). I ...
Mutation, repair, and recombination
... Which of the following statements is correct about the bases in DNA under normal circumstances: • Adenine should pair with thymine. • The bonds holding guanine and cytosine together are stronger than the bonds holding adenine and thymine together. • A purine always binds to a pyrimidine. • A substit ...
... Which of the following statements is correct about the bases in DNA under normal circumstances: • Adenine should pair with thymine. • The bonds holding guanine and cytosine together are stronger than the bonds holding adenine and thymine together. • A purine always binds to a pyrimidine. • A substit ...
Soliton-like base pair opening in a helicoidal DNA: An analogy with
... Electronic address: [email protected] ...
... Electronic address: [email protected] ...
File
... 3. Enzymes that cut DNA molecules at specific places a. have sticky ends. b. are restriction enzymes. c. work only on bacterial DNA. d. always break the DNA between guanine and adenine. ANS: B ...
... 3. Enzymes that cut DNA molecules at specific places a. have sticky ends. b. are restriction enzymes. c. work only on bacterial DNA. d. always break the DNA between guanine and adenine. ANS: B ...
Slide 1
... 1. DNA isolated from tissue sample Small samples can be amplified using another technique called “PCR” 2. DNA cut into fragments with enzymes DNAs of different sequences produce fragments of different sizes 3. Fragments separated on basis of size and visualized 4. Each person’s set of fragments ...
... 1. DNA isolated from tissue sample Small samples can be amplified using another technique called “PCR” 2. DNA cut into fragments with enzymes DNAs of different sequences produce fragments of different sizes 3. Fragments separated on basis of size and visualized 4. Each person’s set of fragments ...
M3 Multiplex Master Mix – PCR (2x)
... but can be individually optimized in a range from 0.2 μM to 0.4 μM per single primer. 5. Color Load: Use of the 10 x Color Load allows PCR reactions to be loaded directly onto an agarose gel without prior addition of a gel loading buffer.The 10 x Color Load contains a gel loading reagent and two gel ...
... but can be individually optimized in a range from 0.2 μM to 0.4 μM per single primer. 5. Color Load: Use of the 10 x Color Load allows PCR reactions to be loaded directly onto an agarose gel without prior addition of a gel loading buffer.The 10 x Color Load contains a gel loading reagent and two gel ...
The splicing factor SR45 affects the RNA
... REPEAT12 (REP12) loci by DNA gel blot and RT-PCR-two loci that are known to be methylated in a DRM2-independent manner.12 We observed that sr45-1 as well as sr45-1, dcl3-1 double mutants showed no difference in methylation when compared with wild type, indicating that SR45 function is most likely co ...
... REPEAT12 (REP12) loci by DNA gel blot and RT-PCR-two loci that are known to be methylated in a DRM2-independent manner.12 We observed that sr45-1 as well as sr45-1, dcl3-1 double mutants showed no difference in methylation when compared with wild type, indicating that SR45 function is most likely co ...
POB3 Is Required for Both Transcription and Replication
... with this, a portion of the total Spt16-Pob3 complex was found to be stably associated with chromatin (Wittmeyer et al. 1999). Spt16-Pob3 bound to affinity matrices containing the catalytic subunit of DNA polymerase ␣ (Pol1) as the ligand (Wittmeyer and Formosa 1995, 1997) and also partially copurif ...
... with this, a portion of the total Spt16-Pob3 complex was found to be stably associated with chromatin (Wittmeyer et al. 1999). Spt16-Pob3 bound to affinity matrices containing the catalytic subunit of DNA polymerase ␣ (Pol1) as the ligand (Wittmeyer and Formosa 1995, 1997) and also partially copurif ...
Detection of two novel porcine herpesviruses with high similarity to
... (Brazelton, 1997). Under these conditions, swine pathogens and even non-pathogenic microorganisms present in pigs might adapt to the immunocompromised human recipient and cause disease or recombine with human viruses to create new pathogens. To avoid such epidemiological risks, pig breeds have to be ...
... (Brazelton, 1997). Under these conditions, swine pathogens and even non-pathogenic microorganisms present in pigs might adapt to the immunocompromised human recipient and cause disease or recombine with human viruses to create new pathogens. To avoid such epidemiological risks, pig breeds have to be ...
Agrobacterium-mediated DNA transfer, and then some
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.