A method for fast database search for all k-nucleotide repeats
... because the basic pattern of the region must be known in advance. In our method, we use this algorithm. More details are provided in section 2.3. In contrast to these other algorithms, our method is straightforward to describe and program. We have produced a working copy which quickly nds tandem re ...
... because the basic pattern of the region must be known in advance. In our method, we use this algorithm. More details are provided in section 2.3. In contrast to these other algorithms, our method is straightforward to describe and program. We have produced a working copy which quickly nds tandem re ...
CBSE XII BIO QS with key(2009-2015)
... the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual reproduction producing seeds without fertilization/ t ...
... the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual reproduction producing seeds without fertilization/ t ...
How Cations Can Assist DNase I in DNA Binding and Hydrolysis
... the conclusion that bpDNase I contains two strong and several weak cation-binding sites [21,22]. More precise information on the number and the location of divalent cations in bpDNase I can be extracted from the numerous high-resolution crystal structures available for this enzyme [23–25]. In partic ...
... the conclusion that bpDNase I contains two strong and several weak cation-binding sites [21,22]. More precise information on the number and the location of divalent cations in bpDNase I can be extracted from the numerous high-resolution crystal structures available for this enzyme [23–25]. In partic ...
Minimum SNPs version 2043 user manual
... completely functional, it is somewhat inconvenient and counter-intuitive to use, with the major issue being that the concatenated data cannot be stored and must be re-assembled from the allele sequence and ST profile data every time the software is used. (Although this only takes a few minutes, it i ...
... completely functional, it is somewhat inconvenient and counter-intuitive to use, with the major issue being that the concatenated data cannot be stored and must be re-assembled from the allele sequence and ST profile data every time the software is used. (Although this only takes a few minutes, it i ...
Bacteriophage A cloning system for the construction of
... libraries. The wild-type A genome contains five BamHI, five EcoRI, and seven Hindu restriction sites that have all been removed from the genome of AORF8. Sites for these endonucleases are present within the multiple cloning site of AORF8. We report a method for preparing cDNAs that can be cloned in ...
... libraries. The wild-type A genome contains five BamHI, five EcoRI, and seven Hindu restriction sites that have all been removed from the genome of AORF8. Sites for these endonucleases are present within the multiple cloning site of AORF8. We report a method for preparing cDNAs that can be cloned in ...
Supplementary Materials and methods (doc 154K)
... assessed. We used the protocol previously described by Itoh et al. for the mobilization of plasmid pNI111 by RP4 (Itoh et al 1991). P. aeruginosa PAO1-GFP (resistant to gentamycin and chloramphenicol) was used as a recipient strain. Mating experiments were performed three independent times on LB aga ...
... assessed. We used the protocol previously described by Itoh et al. for the mobilization of plasmid pNI111 by RP4 (Itoh et al 1991). P. aeruginosa PAO1-GFP (resistant to gentamycin and chloramphenicol) was used as a recipient strain. Mating experiments were performed three independent times on LB aga ...
DNA sequence selectivity of guanine–N7 alkylation by nitrogen
... amine piperidine converts these modified base sites into strand breaks (18). If the DNA is labeled only at one end of one strand, and is of known sequence, the lengths of the labeled fragments produced after alkylation and subsequent alkaline piperidine treatment indicate the position of the origina ...
... amine piperidine converts these modified base sites into strand breaks (18). If the DNA is labeled only at one end of one strand, and is of known sequence, the lengths of the labeled fragments produced after alkylation and subsequent alkaline piperidine treatment indicate the position of the origina ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
... as the selective medium method(Alsinaet al., 1994), API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require cons ...
... as the selective medium method(Alsinaet al., 1994), API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require cons ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... using CRI-4 as the probe. In three of the four individuals having the S allotype, a 14 .5-kb fragment was identified that was absent in individuals homozygous for the F allotype (Fig . 2A). We could find no fragment present in individuals having the F allotype that was absent in the SS individuals, ...
... using CRI-4 as the probe. In three of the four individuals having the S allotype, a 14 .5-kb fragment was identified that was absent in individuals homozygous for the F allotype (Fig . 2A). We could find no fragment present in individuals having the F allotype that was absent in the SS individuals, ...
Gene Detection Systems Catalog
... All submitted online orders receive a message indicating the order number and the email address where the automatic email confirmation will be sent. Please print or make a note of this information. The email confirmation is an official record of order placed and should be saved for future reference. ...
... All submitted online orders receive a message indicating the order number and the email address where the automatic email confirmation will be sent. Please print or make a note of this information. The email confirmation is an official record of order placed and should be saved for future reference. ...
Similarity Searches on Sequence Databases: BLAST
... • Sequence ac number and name: Hyperlink to the database entry: useful annotations • Description: better to check the full annotation • Bit score (normalized score) : A measure of the similarity between the two sequences: the higher the better (matches below 50 bits are very unreliable) • E-value: T ...
... • Sequence ac number and name: Hyperlink to the database entry: useful annotations • Description: better to check the full annotation • Bit score (normalized score) : A measure of the similarity between the two sequences: the higher the better (matches below 50 bits are very unreliable) • E-value: T ...
dna model project - Jessamine County Schools
... Make a fully colored Model of DNA. Your model may be out of any materials that you choose as long as they are not perishable and are sturdy. Please make sure that your model includes at least 16 nucleotides. Each structure should look different from the others, and should clearly show which 2 other ...
... Make a fully colored Model of DNA. Your model may be out of any materials that you choose as long as they are not perishable and are sturdy. Please make sure that your model includes at least 16 nucleotides. Each structure should look different from the others, and should clearly show which 2 other ...
Crimes Act 1914 - Federal Register of Legislation
... material to be included on a national DNA database system. These provisions are also drawn from the 2000 Model Bill and will regulate Commonwealth input into and use of the proposed national DNA database to be established as part of the national CrimTrac initiative. The provisions explain what foren ...
... material to be included on a national DNA database system. These provisions are also drawn from the 2000 Model Bill and will regulate Commonwealth input into and use of the proposed national DNA database to be established as part of the national CrimTrac initiative. The provisions explain what foren ...
Crystal Structure of Paired Domain -...
... 1991; Martin et al. 1992; Krauss et al., 1991; Stapleton et al., 1993; Wallin et al., 1993; Chisholm and Horvitz, submitted). So far at least 30-40 paired-box genes have been cloned based on the sequence homology in the paired-box, including 9 murine Pax genes (Pax-1 to Pax-9) and 9 human PAX genes ...
... 1991; Martin et al. 1992; Krauss et al., 1991; Stapleton et al., 1993; Wallin et al., 1993; Chisholm and Horvitz, submitted). So far at least 30-40 paired-box genes have been cloned based on the sequence homology in the paired-box, including 9 murine Pax genes (Pax-1 to Pax-9) and 9 human PAX genes ...
pyrimidine
... Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesis because the 3’ position is blocked. See figs. 11.3 & 11.4 for how these ar ...
... Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesis because the 3’ position is blocked. See figs. 11.3 & 11.4 for how these ar ...
Role of the p21 Protein in the Fanconi Anemia Pathway
... – If the kinetics of D2 mono-Ub and p21 expression are similar, this would be suggestive that both proteins are involved in the same cellular process. ...
... – If the kinetics of D2 mono-Ub and p21 expression are similar, this would be suggestive that both proteins are involved in the same cellular process. ...
INTERPOL Executive Summary
... Bearing in mind that terrorists and other serious criminals regularly use false documents to travel, INTERPOL established its Stolen/Lost Travel Documents (SLTD) database in 2002. This database has subsequently proven to be an effective tool for intercepting such individuals when attempting to cross ...
... Bearing in mind that terrorists and other serious criminals regularly use false documents to travel, INTERPOL established its Stolen/Lost Travel Documents (SLTD) database in 2002. This database has subsequently proven to be an effective tool for intercepting such individuals when attempting to cross ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... DNA is a molecule composed of deoxyribonucleotides connected by phosphodiester linkages. Genome is the complete DNA sequence of a living organism while gene is a special section of the DNA coding for a protein. The largest publicly accessible nucleotide datasets are maintained in: National Center fo ...
... DNA is a molecule composed of deoxyribonucleotides connected by phosphodiester linkages. Genome is the complete DNA sequence of a living organism while gene is a special section of the DNA coding for a protein. The largest publicly accessible nucleotide datasets are maintained in: National Center fo ...
Mutagenesis Point mutations Deletions Insertions Types of
... with larger DNA fragments, conduct extensive re-engineering of genetic elements, synthesize whole genomes and move towards automated approaches, the technologies required to manipulate DNA also need to evolve. Investigators at the J. Craig Venter Institute (JCVI) have developed a number of in vitro ...
... with larger DNA fragments, conduct extensive re-engineering of genetic elements, synthesize whole genomes and move towards automated approaches, the technologies required to manipulate DNA also need to evolve. Investigators at the J. Craig Venter Institute (JCVI) have developed a number of in vitro ...
Chromatin DNA Methylayion
... Increased MBD2/demethylase is responsible for global hypomethylation and maintaining tumor invasion genes hypomethylated and active ...
... Increased MBD2/demethylase is responsible for global hypomethylation and maintaining tumor invasion genes hypomethylated and active ...
Read the Nobel Lecture
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
Identifying Common Genetic Variants by High
... easiest to distinguish on difference plots (Fig. 1B). Although the identity of the sequence variants was initially not known, samples with melting curves identical to those in a normal population should be benign variants. To test this method of eliminating common variants, we analyzed a blinded pan ...
... easiest to distinguish on difference plots (Fig. 1B). Although the identity of the sequence variants was initially not known, samples with melting curves identical to those in a normal population should be benign variants. To test this method of eliminating common variants, we analyzed a blinded pan ...
Basic 2D gel protocols - Personal Web Site Joel Huberman
... alkaline conditions than bromphenol blue, xylene cyanol, or orange G. Its electrophoretic properties are indistinguishable from those of bromphenol blue. Remember to adjust the ionic conditions of your marker samples so they match those of the corresponding electrophoresis buffers. Also remember to ...
... alkaline conditions than bromphenol blue, xylene cyanol, or orange G. Its electrophoretic properties are indistinguishable from those of bromphenol blue. Remember to adjust the ionic conditions of your marker samples so they match those of the corresponding electrophoresis buffers. Also remember to ...
Nuclear DNA Content Estimates in Multicellular
... Algal material was fixed in Carnoy’s solution and stored in 70 % ethanol at 4 C. Preserved material was rehydrated in water and softened in 5 % w/v EDTA (Goff and Coleman, 1990) for between 30 min and 3 h. Algal specimens were transferred to cover slips treated with subbing solution, air-dried and ...
... Algal material was fixed in Carnoy’s solution and stored in 70 % ethanol at 4 C. Preserved material was rehydrated in water and softened in 5 % w/v EDTA (Goff and Coleman, 1990) for between 30 min and 3 h. Algal specimens were transferred to cover slips treated with subbing solution, air-dried and ...
Finishing the Human Genome
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...