2004-009_-Draft-Anne..
... serological methods may facilitate a presumptive diagnosis of symptomatic plant material; however, an additional test based on a different biological principle should be used for detection. In all tests, positive and negative controls must be included. The use of products of commercial brands in thi ...
... serological methods may facilitate a presumptive diagnosis of symptomatic plant material; however, an additional test based on a different biological principle should be used for detection. In all tests, positive and negative controls must be included. The use of products of commercial brands in thi ...
Specific PCR Primers for the Identification of Salmonella enterica
... using both the conventional culture method (BAM) and the PCR procedure developed in this study. Table 3 shows that a single 535-bp PCR product after PCR amplification could be detected after pre-enrichment from some chicken eggs purchased from market and chicken feces. Herein we found the same resul ...
... using both the conventional culture method (BAM) and the PCR procedure developed in this study. Table 3 shows that a single 535-bp PCR product after PCR amplification could be detected after pre-enrichment from some chicken eggs purchased from market and chicken feces. Herein we found the same resul ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... our model, in which RIF1 promotes origin licensing by directing PP1 to dephosphorylate and stabilize ORC1. The effect is small when looking by western blotting at all cells in the population, and we would have liked to examine the effects in relation to cell cycle stage. We did attempt this experime ...
... our model, in which RIF1 promotes origin licensing by directing PP1 to dephosphorylate and stabilize ORC1. The effect is small when looking by western blotting at all cells in the population, and we would have liked to examine the effects in relation to cell cycle stage. We did attempt this experime ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... studies on effects of gamma-radiation on DNA structure. Around this time it had just been determined that DNA was the hereditary (genetic) material, and the fact that DNA is the molecular bases of heredity peaked Peacocke’s interest in this molecule (Drysdale and Peacocke 1961), although exactly how ...
... studies on effects of gamma-radiation on DNA structure. Around this time it had just been determined that DNA was the hereditary (genetic) material, and the fact that DNA is the molecular bases of heredity peaked Peacocke’s interest in this molecule (Drysdale and Peacocke 1961), although exactly how ...
Current Topics LNA (Locked Nucleic Acid): High
... as observed for RNA‚RNA hybrids. Remarkably, the hybrid with three modifications was shown to adopt a near-canonical A-type duplex geometry, indicating that the LNA nucleotides conformationally tune the DNA nucleotides in the LNA strand to attain N-type sugar puckers (11), as illustrated in Figure 3 ...
... as observed for RNA‚RNA hybrids. Remarkably, the hybrid with three modifications was shown to adopt a near-canonical A-type duplex geometry, indicating that the LNA nucleotides conformationally tune the DNA nucleotides in the LNA strand to attain N-type sugar puckers (11), as illustrated in Figure 3 ...
Epigenetic Regulation ofbdnfGene Transcription in the
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
rec-mediated recombinational hot spot activity in bacteriophage
... strength of the hot spot is sufficiently great that most recombination events occur at the hot spot locus, then the density distribuion of A+R+ recombinants should have a rather sharp peak, as in Figure 2b. For ease of presentation, it will be assumed that all recombination events are equally likely ...
... strength of the hot spot is sufficiently great that most recombination events occur at the hot spot locus, then the density distribuion of A+R+ recombinants should have a rather sharp peak, as in Figure 2b. For ease of presentation, it will be assumed that all recombination events are equally likely ...
Forensic analysis of expirated blood
... with no PCR product being made from human DNA or DNA from soil or other microbes that were tested. It is also very sensitive, detecting as little as 60 fg of target DNA. The PCR was not inhibited by the presence of blood and could detect target DNA in expirated blood for at least 92 days after depos ...
... with no PCR product being made from human DNA or DNA from soil or other microbes that were tested. It is also very sensitive, detecting as little as 60 fg of target DNA. The PCR was not inhibited by the presence of blood and could detect target DNA in expirated blood for at least 92 days after depos ...
Non-homologous end-joining partners in a helical dance: structural
... helical complex structure rather flexible and could also allow the formation of a closed ring structure as in SAS-6. In addition to the proteins bound within the XLF– XRCC4 helical structure, there may be other NHEJ proteins assembled around it interacting with the coiled-coil Cterminal regions as i ...
... helical complex structure rather flexible and could also allow the formation of a closed ring structure as in SAS-6. In addition to the proteins bound within the XLF– XRCC4 helical structure, there may be other NHEJ proteins assembled around it interacting with the coiled-coil Cterminal regions as i ...
Gel immobilization of acrylamide-modified single
... automation [17], this method is comparatively expensive, although the streptavidin-coated magnetic beads can be reused [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ...
... automation [17], this method is comparatively expensive, although the streptavidin-coated magnetic beads can be reused [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ...
Real time PCR and it`s role in diagnosis
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... 10. Cut a square of Parafilm and on it dot 4 ul of loading buffer for each sample, then add 1 ul of PCR product (NB. change tips throughout!). Note that the loading buffer contains one or more tracking dyes that can be seen to migrate through the gel and provides enough density for the PCR sample to ...
... 10. Cut a square of Parafilm and on it dot 4 ul of loading buffer for each sample, then add 1 ul of PCR product (NB. change tips throughout!). Note that the loading buffer contains one or more tracking dyes that can be seen to migrate through the gel and provides enough density for the PCR sample to ...
Localization and structural analysis of the ribosomal RNA operons of
... in the control of ICM induction as well as the synthesis of ICM components. Concurrent with these studies, physical mapping of R. sphaeroides genomic DNA indicated that the arrangement of the R. sphaeroides genome was complex, comprised of a large (containing one rm operon) and small (with two rm op ...
... in the control of ICM induction as well as the synthesis of ICM components. Concurrent with these studies, physical mapping of R. sphaeroides genomic DNA indicated that the arrangement of the R. sphaeroides genome was complex, comprised of a large (containing one rm operon) and small (with two rm op ...
An Introduction to Genetic Engineering: Third Edition
... 1.1 What is genetic engineering? Progress in any scientific discipline is dependent on the availability of techniques and methods that extend the range and sophistication of experiments that may be performed. Over the past 35 years or so this has been demonstrated in a spectacular way by the emergenc ...
... 1.1 What is genetic engineering? Progress in any scientific discipline is dependent on the availability of techniques and methods that extend the range and sophistication of experiments that may be performed. Over the past 35 years or so this has been demonstrated in a spectacular way by the emergenc ...
Questions & Answer keys Test 2 Genetic engg.
... a. RNA is more stickier than DNA with nitrocellulose filter b. Once attached, RNA cannot be separated out from nitrocellulose filter c. RNA forms in bulk and sticks on nitrocellulose filter d. RNA bands do not binds with nitrocellulose filter ...
... a. RNA is more stickier than DNA with nitrocellulose filter b. Once attached, RNA cannot be separated out from nitrocellulose filter c. RNA forms in bulk and sticks on nitrocellulose filter d. RNA bands do not binds with nitrocellulose filter ...
Turning h Cro into a Transcriptional Activator
... the helix 2-3 units of each lie in nearly identical positions on DNA (Hochschild and Ptashne, 1986). Therefore, the two units are positioned to contact the same part of RNA polymerase, and Cro might become an activator if this helix 2-3 unit was suitably modified (Figure 1). We constructed a derivat ...
... the helix 2-3 units of each lie in nearly identical positions on DNA (Hochschild and Ptashne, 1986). Therefore, the two units are positioned to contact the same part of RNA polymerase, and Cro might become an activator if this helix 2-3 unit was suitably modified (Figure 1). We constructed a derivat ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a variety of conformations, however, and there are a variety of dista ...
... diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a variety of conformations, however, and there are a variety of dista ...
1 - IPPC
... contents adjusted to 30% glycerol and stored at –80 ºC for confirmation testing, if necessary. The third tube is kept on ice for performing enrichment before enzyme-linked immunosorbent assay (ELISA) or PCR, and isolation on selective media (Figure 1). If immunofluorescence is to be performed (i.e. ...
... contents adjusted to 30% glycerol and stored at –80 ºC for confirmation testing, if necessary. The third tube is kept on ice for performing enrichment before enzyme-linked immunosorbent assay (ELISA) or PCR, and isolation on selective media (Figure 1). If immunofluorescence is to be performed (i.e. ...
Reclassification of Koreibacter algae as a later heterotypic synonym
... Micrococcineae) were isolated independently from marine habitats by two research groups without being aware that the two organisms were taxonomically highly similar. One group submitted the proposal for a new genus and species Paraoerskovia marina (Khan et al., 2009), whereas the other named the nov ...
... Micrococcineae) were isolated independently from marine habitats by two research groups without being aware that the two organisms were taxonomically highly similar. One group submitted the proposal for a new genus and species Paraoerskovia marina (Khan et al., 2009), whereas the other named the nov ...
Rotaphor System 6.0
... The Rotaphor® System contains no user serviceable parts. Do not open the instruments housing. Service and repair may only be carried out by the Biometra Service department or otherwise ...
... The Rotaphor® System contains no user serviceable parts. Do not open the instruments housing. Service and repair may only be carried out by the Biometra Service department or otherwise ...
f o r e n s i c science - and
... obvious. If a sexual assault is oral, digital, or utilizing a foreign object, then it is useful to determine the details associated with the alleged assault to process the evidence most effectively. In sexual assault cases where semen is not alleged, examining an item for the presence of semen may h ...
... obvious. If a sexual assault is oral, digital, or utilizing a foreign object, then it is useful to determine the details associated with the alleged assault to process the evidence most effectively. In sexual assault cases where semen is not alleged, examining an item for the presence of semen may h ...
Optimal estimation of diffusion coefficients from single
... These proofs are not valid here, however, because both these estimators depend nonlinearly on the parameters of interest—the diffusion coefficient D and the variance σ 2 of localization errors—and we show below that OLSF and GLS are suboptimal. The complicated dependence of the MSDs on data makes it ...
... These proofs are not valid here, however, because both these estimators depend nonlinearly on the parameters of interest—the diffusion coefficient D and the variance σ 2 of localization errors—and we show below that OLSF and GLS are suboptimal. The complicated dependence of the MSDs on data makes it ...
DNA-mediated transformation of the filamentous fungusAspergillus
... In diploid nuclei mitotic crossing-over may occur, however its frequency is low and consequently, following haploidization, in general not more than one cross-over event is detected in about 200 haploids (Pontecorvo and Käfer 1958). The alternation of haploid and diploid stages in the absence of mei ...
... In diploid nuclei mitotic crossing-over may occur, however its frequency is low and consequently, following haploidization, in general not more than one cross-over event is detected in about 200 haploids (Pontecorvo and Käfer 1958). The alternation of haploid and diploid stages in the absence of mei ...