Compaction of Duplex Nucleic Acids upon Native
... > 12-d100 (supporting Figure S14), in line with the relative gas-phase kinetic stabilities in tandem mass spectrometry.11-14 When LC models are used, the T-REMD final structures depend significantly on the choice of charge location (Figure S17, S19—S21). For example, for [12d100]5-, a first model (F ...
... > 12-d100 (supporting Figure S14), in line with the relative gas-phase kinetic stabilities in tandem mass spectrometry.11-14 When LC models are used, the T-REMD final structures depend significantly on the choice of charge location (Figure S17, S19—S21). For example, for [12d100]5-, a first model (F ...
SYBR Green I Nucleic Acid Gel Stain
... Schuell, Inc., Dark Reader is a trademark of Clare Chemical Research, Inc., Polaroid is a trademark of Polaroid Corp., Rubbermaid is a trademark of Rubbermaid Prod., Tiffen is a trademark of Tiffen Manufacturing, Wratten is a trademark of Eastman Kodak Co.. SYBR Green Stain is covered by U.S. Patent ...
... Schuell, Inc., Dark Reader is a trademark of Clare Chemical Research, Inc., Polaroid is a trademark of Polaroid Corp., Rubbermaid is a trademark of Rubbermaid Prod., Tiffen is a trademark of Tiffen Manufacturing, Wratten is a trademark of Eastman Kodak Co.. SYBR Green Stain is covered by U.S. Patent ...
15.13 Spm elements influence gene expression
... location in the genome (without any sequence relationship with the target locus). ...
... location in the genome (without any sequence relationship with the target locus). ...
Figure 15.6 Nonreplicative transposition allows a transposon to
... genome (without any sequence relationship with the target locus). ...
... genome (without any sequence relationship with the target locus). ...
The physics behind the larger scale organization of DNA in eukaryotes
... discrepancy can be attributed to the approximations involved in the model that start to matter at such small fiber diameters. 2.3. Energetics: elasticity and electrostatics One important property we need to address is the energetics involved in the models. It is usually inferred from experiments tha ...
... discrepancy can be attributed to the approximations involved in the model that start to matter at such small fiber diameters. 2.3. Energetics: elasticity and electrostatics One important property we need to address is the energetics involved in the models. It is usually inferred from experiments tha ...
Resolving the Discrepancies Among Nucleic Acid Conformational
... folding of nucleic acids and the sequence-dependent structure of individual base-pair steps has stimulated the development of new mathematical methods to de®ne the geometry of the constituent base-pairs. Several approaches, designed to meet guidelines set by the nucleic acid community, permit rigoro ...
... folding of nucleic acids and the sequence-dependent structure of individual base-pair steps has stimulated the development of new mathematical methods to de®ne the geometry of the constituent base-pairs. Several approaches, designed to meet guidelines set by the nucleic acid community, permit rigoro ...
A Complete Mitochondrial Genome Sequence
... Background: The derivation of domestic cattle from the extinct wild aurochs (Bos primigenius) has been well-documented by archaeological and genetic studies. Genetic studies point towards the Neolithic Near East as the centre of origin for Bos taurus, with some lines of evidence suggesting possible, ...
... Background: The derivation of domestic cattle from the extinct wild aurochs (Bos primigenius) has been well-documented by archaeological and genetic studies. Genetic studies point towards the Neolithic Near East as the centre of origin for Bos taurus, with some lines of evidence suggesting possible, ...
Rapid and accurate cancer somatic mutation
... background and on 100% mutant template by a mutation assay. Assays were verified to have a minimal ADW of 8 but are usually higher. B Average and median assay differentiation window, and average, median assay sensitivities based on 406 qBiomarker Somatic Mutation PCR Assays. C Assay sensitivity test ...
... background and on 100% mutant template by a mutation assay. Assays were verified to have a minimal ADW of 8 but are usually higher. B Average and median assay differentiation window, and average, median assay sensitivities based on 406 qBiomarker Somatic Mutation PCR Assays. C Assay sensitivity test ...
Document
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... normalized data into a ratio or polar coordinates. For simplicity, we will utilize a ratio transformation Yi = Ai/(Ai+kiBi), where Ai is the probe intensity for the A allele and B is the probe intensity for the B allele for the jth SNP. Multiple papers have shown that Yj transformation approximates ...
... normalized data into a ratio or polar coordinates. For simplicity, we will utilize a ratio transformation Yi = Ai/(Ai+kiBi), where Ai is the probe intensity for the A allele and B is the probe intensity for the B allele for the jth SNP. Multiple papers have shown that Yj transformation approximates ...
Aberrant DNA methylation in cancer: potential clinical
... tumour was first reported in 1986 (Ref. 19). However, it was not until recently, particularly following the advent of polymerase chain reaction (PCR)-based techniques for methylation analysis (Ref. 20), that analysis of CpG island methylation in tumours has become widespread. In the past few years, ...
... tumour was first reported in 1986 (Ref. 19). However, it was not until recently, particularly following the advent of polymerase chain reaction (PCR)-based techniques for methylation analysis (Ref. 20), that analysis of CpG island methylation in tumours has become widespread. In the past few years, ...
Structure, stability and function of 5-chlorouracil
... bacteria was <2% and the switch from T to ClU was accompanied by a massive number of mutations, including >1500 A to G or G to A transitions in a culture. The former is most likely due to wobble base pairing between ClU and G, which may be more common for ClU than T. To identify potential changes in ...
... bacteria was <2% and the switch from T to ClU was accompanied by a massive number of mutations, including >1500 A to G or G to A transitions in a culture. The former is most likely due to wobble base pairing between ClU and G, which may be more common for ClU than T. To identify potential changes in ...
Manual: FullVelocity(TM) SYBR® Green QPCR - Gene X
... For best results, design primers that amplify PCR products 90–300 bp in length. The primers should exhibit a melting temperature (Tm) of approximately 60°C, which is the annealing-extension temperature of the FullVelocity amplification reaction. It is critical to minimize the formation of non-specif ...
... For best results, design primers that amplify PCR products 90–300 bp in length. The primers should exhibit a melting temperature (Tm) of approximately 60°C, which is the annealing-extension temperature of the FullVelocity amplification reaction. It is critical to minimize the formation of non-specif ...
A Community-Based Annotation Framework for
... The amount of biological data available in the public domain is growing exponentially, and there is an increasing need for infrastructural and human resources to organize, store, and present the data in a proper context. Model organism databases (MODs) invest great efforts to functionally annotate g ...
... The amount of biological data available in the public domain is growing exponentially, and there is an increasing need for infrastructural and human resources to organize, store, and present the data in a proper context. Model organism databases (MODs) invest great efforts to functionally annotate g ...
Sex differences in the genome-wide DNA
... Background: Epigenetic factors regulate tissue-specific expression and X-chromosome inactivation. Previous studies have identified epigenetic differences between sexes in some human tissues. However, it is unclear whether epigenetic modifications contribute to sex-specific differences in insulin sec ...
... Background: Epigenetic factors regulate tissue-specific expression and X-chromosome inactivation. Previous studies have identified epigenetic differences between sexes in some human tissues. However, it is unclear whether epigenetic modifications contribute to sex-specific differences in insulin sec ...
Functions of DNA methylation: islands, start sites, gene bodies and
... CGIs remain unmethylated in somatic cells. When genes with CGIs at their TSS are active, their promoters are usually characterized by nucleosome-depleted regions (NDRs) at the TSS, and these NDRs are often flanked by nucleosomes containing the histone variant H2A.Z and are marked with trimethylation ...
... CGIs remain unmethylated in somatic cells. When genes with CGIs at their TSS are active, their promoters are usually characterized by nucleosome-depleted regions (NDRs) at the TSS, and these NDRs are often flanked by nucleosomes containing the histone variant H2A.Z and are marked with trimethylation ...
Review A model for chromosome structure during the mitotic
... DNA when they come under tension as the sister chromatids begin to shorten and separate in prophase (Earnshaw & Heck 1985, Woessner et al. 1987, Saitoh & Laemmli 1994, Gimënez-Abiän et al. 1995). It is also during S phase when cohesin complexes establish linkage between sister chromatids (Uhlmann ...
... DNA when they come under tension as the sister chromatids begin to shorten and separate in prophase (Earnshaw & Heck 1985, Woessner et al. 1987, Saitoh & Laemmli 1994, Gimënez-Abiän et al. 1995). It is also during S phase when cohesin complexes establish linkage between sister chromatids (Uhlmann ...
An accessible database for mouse and human whole transcriptome
... We created databases for primer pairs to be used for amplification of intron–exon and exon–exon junctions by qPCR, genomewide, for human and mouse; in total, 534 301 pairs for human (and 518 230 for mouse) targeting 17 929 (and 18 039) different genes in human (and mouse). Many of the primer pairs i ...
... We created databases for primer pairs to be used for amplification of intron–exon and exon–exon junctions by qPCR, genomewide, for human and mouse; in total, 534 301 pairs for human (and 518 230 for mouse) targeting 17 929 (and 18 039) different genes in human (and mouse). Many of the primer pairs i ...
SURVEY AND SUMMARY New insights into Hoogsteen base pairs
... while, in contrast, G•C derivatives co-crystallized into the expected WC bps (6). The lack of experimental evidence for A•T/U WC bps raised considerable scepticism about the WC base-pairing scheme during 1960s and early 1970s as reviewed in (7). Computational studies on A•T base derivatives later sh ...
... while, in contrast, G•C derivatives co-crystallized into the expected WC bps (6). The lack of experimental evidence for A•T/U WC bps raised considerable scepticism about the WC base-pairing scheme during 1960s and early 1970s as reviewed in (7). Computational studies on A•T base derivatives later sh ...
Clin Infect Dis. - Repositorio Académico UPC
... bacterial load of EPEC isolated from stool samples from children with and without diarrhea to determine whether bacterial load might be a useful tool for further study of this phenomenon. Methods. EPEC was detected by polymerase chain reaction (PCR) of colonies isolated on MacConkey plates from 53 d ...
... bacterial load of EPEC isolated from stool samples from children with and without diarrhea to determine whether bacterial load might be a useful tool for further study of this phenomenon. Methods. EPEC was detected by polymerase chain reaction (PCR) of colonies isolated on MacConkey plates from 53 d ...
APOBEC3A deaminates transiently exposed single-strand DNA
... Transposable elements are grouped by how they move to new locations in a genome. Some move by a cut-and-paste mechanism—whereby the transposable element DNA is removed from one location and inserted back at a new genomic location. Others, termed retrotransposons, move by a copy-and-paste mechanism: ...
... Transposable elements are grouped by how they move to new locations in a genome. Some move by a cut-and-paste mechanism—whereby the transposable element DNA is removed from one location and inserted back at a new genomic location. Others, termed retrotransposons, move by a copy-and-paste mechanism: ...
SALSA MLPA KIT ME003-A1 Tumor suppressor-3 - MRC
... promotor regions of 18 different tumour suppressor genes. These tumour suppressor genes are frequently silenced by methylation in tumours, but are unmethylated in blood-derived DNA of healthy individuals. In addition, 13 reference probes are included which are not affected by HhaI digestion. Besides ...
... promotor regions of 18 different tumour suppressor genes. These tumour suppressor genes are frequently silenced by methylation in tumours, but are unmethylated in blood-derived DNA of healthy individuals. In addition, 13 reference probes are included which are not affected by HhaI digestion. Besides ...
An Introduction to Peptide Nucleic Acid
... although abstract at the point of conception. The pursuit of a neutral backbone drove the design, and the leap to peptide (or amide) chemistry was easy because of the well-established robustness and flexibility of solid phase peptide synthesis (SPPS) technology. During the early stages of the design ...
... although abstract at the point of conception. The pursuit of a neutral backbone drove the design, and the leap to peptide (or amide) chemistry was easy because of the well-established robustness and flexibility of solid phase peptide synthesis (SPPS) technology. During the early stages of the design ...
DNA Methylation Analysis
... bead-bound probes are used to detect the presence of T or C by hybridization followed by single-base extention with a labeled nucleotide. ...
... bead-bound probes are used to detect the presence of T or C by hybridization followed by single-base extention with a labeled nucleotide. ...
functional analysis of chromatin assembly genes in tetrahymena
... Fillingham for providing me with an exceptional opportunity to grow and gain knowledge in the field of molecular biology under his supportive guidance. I am also extremely thankful to Dr.Jyoti Garg who provided me with exceptional insights and assistance throughout the depth of my thesis. I would li ...
... Fillingham for providing me with an exceptional opportunity to grow and gain knowledge in the field of molecular biology under his supportive guidance. I am also extremely thankful to Dr.Jyoti Garg who provided me with exceptional insights and assistance throughout the depth of my thesis. I would li ...