THE CENTRAL DOGMA OF MOLECULAR BIOLOGY Jony Mallik B
... fragments of new DNA in the 5' → 3' direction. These fragments are called Okazaki fragments, which are later joined to form a continuous chain of nucleotides. This strand is known as the lagging strand since the process of DNA synthesis on this strand proceeds at a lower rate. LIGATION & TERMINATION ...
... fragments of new DNA in the 5' → 3' direction. These fragments are called Okazaki fragments, which are later joined to form a continuous chain of nucleotides. This strand is known as the lagging strand since the process of DNA synthesis on this strand proceeds at a lower rate. LIGATION & TERMINATION ...
DNA - South Gibson Science
... DNA – deoxyribonucleic acid is the nucleic acid that stores and transmits genetic info. from one generation to the next. ...
... DNA – deoxyribonucleic acid is the nucleic acid that stores and transmits genetic info. from one generation to the next. ...
Slide 1
... • Replication bubble – an area in which DNA replication is occurring with both strands unwound and being used as templates • Replication fork – the end of a replication bubble in which DNA polymerase is actively adding nucleotide bases to a new strand • DNA polymerase – an enzyme that adds new nucle ...
... • Replication bubble – an area in which DNA replication is occurring with both strands unwound and being used as templates • Replication fork – the end of a replication bubble in which DNA polymerase is actively adding nucleotide bases to a new strand • DNA polymerase – an enzyme that adds new nucle ...
How to Extract DNA
... If no DNA appeared in your glass it may be because the experiment took too long. Try doing it again but a bit more quickly. Try a different combination of ingredients and you may get better results. Using a different brand of bottled water or detergent changed our results. Trying a different type of ...
... If no DNA appeared in your glass it may be because the experiment took too long. Try doing it again but a bit more quickly. Try a different combination of ingredients and you may get better results. Using a different brand of bottled water or detergent changed our results. Trying a different type of ...
E. coli
... Three sets of researchers provided the data that once and for all settled the question 1928: Fredrick Griffith experimented on transformation 1944: Oswald Avery, Colin MacLeod, & Maclyn McCarty repeated Griffith’s experiments, but added further ...
... Three sets of researchers provided the data that once and for all settled the question 1928: Fredrick Griffith experimented on transformation 1944: Oswald Avery, Colin MacLeod, & Maclyn McCarty repeated Griffith’s experiments, but added further ...
Chapter 10 retake assignment with KEY
... nucleotides on opposite strands. The nitrogenous bases form hydrogen bonds with one another in pairs. Adenine forms hydrogen bonds with thymine, and guanine forms hydrogen bonds with cytosine. 71. ANS: DNA replication begins with the two DNA strands separating and unwinding. Each strand serves as a ...
... nucleotides on opposite strands. The nitrogenous bases form hydrogen bonds with one another in pairs. Adenine forms hydrogen bonds with thymine, and guanine forms hydrogen bonds with cytosine. 71. ANS: DNA replication begins with the two DNA strands separating and unwinding. Each strand serves as a ...
Document
... Cells Divide by the Process of Mitosis Cell with a single copy of DNA DNA replicates to form chromosomes (two copies of DNA) Chromosomes line up in the middle of the cell Chromosomes are split. Half of each chromosome travels to either end of the cell. The cell divides to form two new cells with th ...
... Cells Divide by the Process of Mitosis Cell with a single copy of DNA DNA replicates to form chromosomes (two copies of DNA) Chromosomes line up in the middle of the cell Chromosomes are split. Half of each chromosome travels to either end of the cell. The cell divides to form two new cells with th ...
pcr
... How you will determine your target sequence? How the amplification will be specific for certain segment? PCR does not copy all of the DNA in the sample. It copies only a very specific sequence of genetic code, targeted by the PCR primers ...
... How you will determine your target sequence? How the amplification will be specific for certain segment? PCR does not copy all of the DNA in the sample. It copies only a very specific sequence of genetic code, targeted by the PCR primers ...
DNA structure lab protocol
... chemical building blocks called “nucleotides.” There are four different nucleotides, which are labeled adenine (A), thymine (T), guanine (G), and cytosine (C). The human genome is made of a sequence of roughly three billion of these nucleotides. The genome is like a library of instructions. A gene i ...
... chemical building blocks called “nucleotides.” There are four different nucleotides, which are labeled adenine (A), thymine (T), guanine (G), and cytosine (C). The human genome is made of a sequence of roughly three billion of these nucleotides. The genome is like a library of instructions. A gene i ...
What is DNA?
... - Codon = 3 nitrogen bases on DNA or mRNA. Each codon represents 1 of 20 amino acids. • START CODON – AUG codes for the amino acid Methionine ...
... - Codon = 3 nitrogen bases on DNA or mRNA. Each codon represents 1 of 20 amino acids. • START CODON – AUG codes for the amino acid Methionine ...
COA: GeneRuler 100 bp DNA Ladder , #SM0241
... © 2011 Thermo Fisher Scientific Inc. All rights reserved. SYBR is a registered trademark of Molecular Probes, Inc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. ...
... © 2011 Thermo Fisher Scientific Inc. All rights reserved. SYBR is a registered trademark of Molecular Probes, Inc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. ...
DNA replication and protein synthesis
... • RNA primer attaches to leading strand to give it a “starting point” • DNA polymerase adds complementary nucleotides (A-T and C-G) from 3 to 5 prime end of leading parent strand (so is really creating the opposite or a 5 prime strand) • The DNA polymerase can only add bases to the 3’ end of the str ...
... • RNA primer attaches to leading strand to give it a “starting point” • DNA polymerase adds complementary nucleotides (A-T and C-G) from 3 to 5 prime end of leading parent strand (so is really creating the opposite or a 5 prime strand) • The DNA polymerase can only add bases to the 3’ end of the str ...
RNA
... RNA Polymerases Take Instructions from DNA Templates • Base Composition (Viral DNA vs. Viral RNA) • Hybridization Experiments between DNA template and transcribed RNA • Sequence Comparison between RNA and DNA templates (template strand vs. coding strand; anti-sense strand vs. sense strand) ...
... RNA Polymerases Take Instructions from DNA Templates • Base Composition (Viral DNA vs. Viral RNA) • Hybridization Experiments between DNA template and transcribed RNA • Sequence Comparison between RNA and DNA templates (template strand vs. coding strand; anti-sense strand vs. sense strand) ...
Document
... The Cell Cycle 1. Cell Growth (G1) Phase: When cells do most of their growing. – Cells that are not dividing or preparing to divide remain in this phase, but it is called G0! 2. DNA Replication (S) Phase: Chromosomes are replicated and the synthesis of DNA molecules takes place. 3. Preparation for ...
... The Cell Cycle 1. Cell Growth (G1) Phase: When cells do most of their growing. – Cells that are not dividing or preparing to divide remain in this phase, but it is called G0! 2. DNA Replication (S) Phase: Chromosomes are replicated and the synthesis of DNA molecules takes place. 3. Preparation for ...
DNA - HCC Learning Web
... • RNA molecules usually exist as single pnucleotide chains • DNA molecules have two polynucleotides spiraling around an imaginary axis, forming a double helix • In the DNA double helix, the two backbones run in opposite 5→ 3 directions from each other, an arrangement referred to as antiparallel • ...
... • RNA molecules usually exist as single pnucleotide chains • DNA molecules have two polynucleotides spiraling around an imaginary axis, forming a double helix • In the DNA double helix, the two backbones run in opposite 5→ 3 directions from each other, an arrangement referred to as antiparallel • ...
Option B8 Nucleic Acids
... stranded DNA into single strands 6.Copy of the strands is transferred to a membrane and selected radioactively labeled DNA probes are added to the membrane to base pair with particular DNA sequences. Excess washed away. 7.Membrane is overlaid with X-ray film which becomes selectively ‘fogged’ by ...
... stranded DNA into single strands 6.Copy of the strands is transferred to a membrane and selected radioactively labeled DNA probes are added to the membrane to base pair with particular DNA sequences. Excess washed away. 7.Membrane is overlaid with X-ray film which becomes selectively ‘fogged’ by ...
Genetics mini-quiz
... 1. Griffith was known for describing/discovering which of the following? a. Transformation b. DNA structure c. DNA is genetic material d. Genes are on chromosomes e. Transforming material is DNA 2. T.H. Morgan was known for describing/discovering which of the following? a. Transformation b. DNA stru ...
... 1. Griffith was known for describing/discovering which of the following? a. Transformation b. DNA structure c. DNA is genetic material d. Genes are on chromosomes e. Transforming material is DNA 2. T.H. Morgan was known for describing/discovering which of the following? a. Transformation b. DNA stru ...
Name________________________________ Date___________
... b. helicases and single strand binding proteins work at the 5’ end c. DNA polymerase can join new nucleotides only to the 3’ end of a growing strand d. DNA ligase works only in the 3’5’ direction 16. In analyzing the number of different bases in a DNA sample, which result would be consistent with t ...
... b. helicases and single strand binding proteins work at the 5’ end c. DNA polymerase can join new nucleotides only to the 3’ end of a growing strand d. DNA ligase works only in the 3’5’ direction 16. In analyzing the number of different bases in a DNA sample, which result would be consistent with t ...
Unit 13: Review Biotechnology Lab
... Restriction endonucleases or restriction enzymes (REs) are enzymes obtained by bacteria that physically cut DNA. REs are named according to the following guidelines. The first letter is the same letter as the first letter of the genus name of the organism from which is was isolated and is italicized ...
... Restriction endonucleases or restriction enzymes (REs) are enzymes obtained by bacteria that physically cut DNA. REs are named according to the following guidelines. The first letter is the same letter as the first letter of the genus name of the organism from which is was isolated and is italicized ...
Structure of DNA
... Crick, 1953: all were involved in discovering the structure of DNA. Franklin and Wilkins worked in an x-ray crystallography lab, where molecules are crystallized and an x-ray image of it can be taken. Franklin was one of the first to be able to crystallize DNA, and captured the photo that gave Watso ...
... Crick, 1953: all were involved in discovering the structure of DNA. Franklin and Wilkins worked in an x-ray crystallography lab, where molecules are crystallized and an x-ray image of it can be taken. Franklin was one of the first to be able to crystallize DNA, and captured the photo that gave Watso ...
Document
... The process can be broken down into three steps. Step 1: Before replication can begin, the double helix must unwind. This is accomplished by enzymes called DNA helicases, which open up the double helix by breaking the hydrogen bonds that link the complementary nitrogenous bases. Once the two strands ...
... The process can be broken down into three steps. Step 1: Before replication can begin, the double helix must unwind. This is accomplished by enzymes called DNA helicases, which open up the double helix by breaking the hydrogen bonds that link the complementary nitrogenous bases. Once the two strands ...
Chapter 16
... Replication begins at particular sites called origins of replication, where the two DNA strands are separated, opening up a replication “bubble” ...
... Replication begins at particular sites called origins of replication, where the two DNA strands are separated, opening up a replication “bubble” ...
Lab 11
... Use the DNA puzzle to build a DNA, transcribe it into messenger RNA and start to build a protein. Note that you may want to use part of each model in the next one you build. DNA model 1. Sort out the building blocks for DNA from the other pieces. These are: --deoxyribose sugars (salmon) --phosphates ...
... Use the DNA puzzle to build a DNA, transcribe it into messenger RNA and start to build a protein. Note that you may want to use part of each model in the next one you build. DNA model 1. Sort out the building blocks for DNA from the other pieces. These are: --deoxyribose sugars (salmon) --phosphates ...
DNA replication
DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.