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DNA Replication and Protein Synthesis Virtual Lab
DNA Replication and Protein Synthesis Virtual Lab

... http://tinyurl.com/7qt6wwu 1. Most organisms have the same sort of very molecule ...
DNA Replication, Repair, and Recombination
DNA Replication, Repair, and Recombination

... Termination of replication Large 350 kb region in E. coli genome Flanked by 7 nonpalindromic nearly identical termination Sites Replication fork counterclockwise passes through TerG,F, B, and C but stops at TerA Analogous for other direction Ter act as valves Ter-action requires binding of Tus prot ...
14.1 Structure of Ribonucleic Acid (RNA)
14.1 Structure of Ribonucleic Acid (RNA)

... • Each amino acid in a protein is coded for by a sequence of three nucleotide bases on the mRNA strand. • These sequences of three nucleotide bases are known as CODONS. • The triplet code is referred to as degenerate. – this is because most amino acids have more than one ...
CELL DIVISION
CELL DIVISION

... Multiple Forks • Replication does not begin at one end of the DNA molecule and end at the other. • Each chromosome contains a single long strand of DNA. • The replication of a typical human chromosome with one pair of replication forks would take 33 days! • For this reason, each human chromosome is ...
Structure of a six-finger transcription factor IIIA complex
Structure of a six-finger transcription factor IIIA complex

... * Six-finger protein stretches along the entire length of the 31-bp duplex * The current protein model includes amino acids 10–188 of TFIIIA * fingers 1–2–3 adopt a completely different configuration than do fingers 4–5–6 (A) ...
Deoxyribonucleic acid, DNA
Deoxyribonucleic acid, DNA

... Composed of a four-letter nucleotide/molecule alphabet referred to as A, T, C, and G. Order of the alphabet determines the characteristics of the living organism, much like the order of letters in our alphabet determines the words. Each cell in the human body contains >3 BILLION letters. The only di ...
Extracting DNA from Cells
Extracting DNA from Cells

... – Oil and water do not mix. If I have something that will dissolve in water and I shake it with oil, it will stay in the water layer. – If I put something in the water layer that is more soluble in oil, if I shake oil and water together it will transfer to the oil layer. I have extracted it from the ...
Camp 1 - University of California, Santa Cruz
Camp 1 - University of California, Santa Cruz

... • Once the two strands have separated at the replication fork, the nucleotides must be lined up in proper order for DNA synthesis. • In the absence of DNA polymerase, alignment is slow. • DNA polymerase provides the speed and specificity of alignment. • Along lagging (3’ -> 5’) strand, polymerases c ...
12.1 DNA
12.1 DNA

... The Shape of DNA is a Double Helix  DNA often is compared to a twisted ladder.  Rails of the ladder are represented by the alternating deoxyribose and phosphate.  The pairs of bases (cytosine–guanine or thymine–adenine) form the steps. ...
Lecture 14: Nucleic Acids and DNA Replication
Lecture 14: Nucleic Acids and DNA Replication

... (iii) Suggests a general mechanism for DNA replication--bases form specific pairs, therefore the information in one strand compliments the other IV. ...
BIOL212aluIntronLabSpr2012
BIOL212aluIntronLabSpr2012

... Sketches needn’t be art! • Many of your labs in 212 will ask that you draw sketches of what you are seeing. In addition, some of the labs we do will ask you to graph data and draw conclusions. This work, too, should be in your lab notebook, even if your instructor asks you to prepare a separate, mo ...
Deoxyribonucleic Acid
Deoxyribonucleic Acid

... • Most people still did not believe DNA could be responsible for genetics because there are only 4 different bases (nucleotides) while proteins are made of 20 different bases (amino acids). • How could a 4 letter “alphabet” account for the differences between living things? ...
A - sandsbiochem
A - sandsbiochem

... 29. Individuals with one form of lactose intolerance do not produce the enzyme lactase because the gene coding for the production of lactase is shut off in their cells. This means that which of the following processes does not occur for the gene? a. Hydrogenation c. replication b. mutation d. transc ...
Unit 6 Notes
Unit 6 Notes

... What: Changing DNA to RNA Where: Nucleus When: All the time ...
word - marric
word - marric

... make up the _________________________ that keeps the enzyme in place. (4) _____________________ removes the RNA primers that will be replaced by ___________________ (5) The DNA fragments are bound together by the enzyme ______________________. 2. Students can explain why DNA Replication is described ...
Better Crush and Soak, than Crash and Burn!
Better Crush and Soak, than Crash and Burn!

... present in Buffer QG. The denaturing effect of Buffer QG is worse, when DNA fragments are short and AT rich. Moreover, high temperatures and long incubation times increase the denaturing effect of Buffer QG. Since ethidium bromide does not bind to single-stranded DNA, it may be difficult to notice s ...
Chapter 12 HW Packet
Chapter 12 HW Packet

... to make the other strand, the strands are said to be complementary. DNA copies itself through the process of replication: The two strands of the double helix unzip, forming replication forks. New bases are added, following the rules of base pairing (A with T and G with C). Each new DNA molecule has ...
PROTEIN SYNTHESIS
PROTEIN SYNTHESIS

... • 2. Translation - “put recipe together” • 3. Elongation - “glue” amino acids together to form protein ...
RNA - Burlington Township School District
RNA - Burlington Township School District

... RNA is similar to DNA, but there are 3 major differences.  RNA is single stranded, DNA is double  Ribose is the sugar instead of Deoxyribose  Cytosine, Guanine, Adenine and Uracil (instead of Thymine) ...
DNA Polymerase
DNA Polymerase

... - an mRNA is synthesized using complementary base pairing with uracil (U) replacing thymine (T) - the newly formed mRNA moves out of the nucleus to ribosomes in the cytoplasm and the DNA re-winds ...
Solutions: Chapter 4 and 5 Review Sheet
Solutions: Chapter 4 and 5 Review Sheet

... 16.  The  seven  stages,  which  break  down  the  events  of  DNA  replication,  are  listed  below:     i. The  enzyme  gyrase  relieves  any  tension  from  the  unwinding  double  helix.     ii. The  enzyme  helicase  breaks  the ...
LECTURE #3: DNA History and Scientists
LECTURE #3: DNA History and Scientists

... • Deoxyribose nucleic acid  type of nucleic acid – What is the other type of nucleic acid? • RNA • DNA function – to hold genetic code – Genetic code = genetic instructions to make proteins • DNA is found in nucleus of eukaryotic cells • Found in nucleoid region in prokaryotes ...
DNA
DNA

... DNA helicases unwind double helix by breaking hydrogen bonds Proteins attach to each strand to hold them apart and prevent them from twisting back This area is called a replication fork ...
DNA - Henrico
DNA - Henrico

... The bacteriophage injects its DNA into the cell. The viral genes act to produce many new bacteriophages, which burst out when the cell splits open. ...
Unit 5 Molecular Genetics CLASS NOTES
Unit 5 Molecular Genetics CLASS NOTES

... http://sciencenetlinks.com/media/filer/2011/10/13/dna.swf ...
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DNA replication



DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.
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