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Chapter 4
Chapter 4

... Shift to 14NH4Cl medium Purify DNA Gradient sedimentation ...
No Slide Title
No Slide Title

... – 3’ UTRs – 5’ UTRs …these are “outside” the exon sequences. ...
Ravi Sundaram What is PCR Why is it such a major breakthrough?
Ravi Sundaram What is PCR Why is it such a major breakthrough?

... segments carrying this genetic information are called genes. DNA consists of two long polymers of simple units called nucleotides. These two strands run in opposite directions to each other. Each nucleotide contains one of four types of molecules called bases (or nucleobases or nucleic acids). These ...
How many tetrads are there in metaphase I of
How many tetrads are there in metaphase I of

... Yellowstone National Park. When you examine the nucleotide composition of this organism, you find that 10% of the nucleotides in its DNA are adenine. What percentage of nucleotides are guanine? Explain. A. 10%, because A pairs with G B. 40%, because A pairs with T (accounting for 20% of the bases), ...
DNA: Structure, Function, and History
DNA: Structure, Function, and History

... is a double helix (but they had help along the way!) ...
DNA, RNA & PROTEIN SYNTHESIS
DNA, RNA & PROTEIN SYNTHESIS

... Nucleotides found in the surrounding nucleoplasm join to their base pair by enzymes known as POLYMERASES. Hydrogen bonds form between the bases. When each of the strands is remade, two IDENTICAL strands are made with ONE OLD strand and ONE NEW strand = SEMICONSERVATIVE REPLICATION ...
Molecular Biology what are proteins? what are the building blocks of
Molecular Biology what are proteins? what are the building blocks of

... DNA→transcription→RNA→translation → protein translation: location - ribosomes in cytoplasm or on ER enzymes - none, uses tRNA - brings amino acids and rRNA - ribosomes to combine amino acids sites: codon - set of 3 nucleotides on mRNA, match up with anticodon - set of 3 nucleotides on tRNA start cod ...
Modeling Activity: How Genes Make Proteins…
Modeling Activity: How Genes Make Proteins…

... 5. As each tRNA anticodon finds its corresponding codon on the mRNA strand, the tRNA will detach from its amino acid. The amino acids will remain at the ribosome and form a peptide bond with the next amino acid brought by another tRNA. Two or more amino acids bonded together are called a polypeptide ...
THE NUCLEIC ACIDS
THE NUCLEIC ACIDS

... • DNA is a double-stranded helix, with th two strands connected by hydrogen bonds. • Most DNA double helices are righthanded; • The DNA double helix is anti-parallel, which means that the 5' end of one strand is paired with the 3' end of its complementary strand (and vice versa) • Nucleotides are l ...
chapter_8_mod_2009
chapter_8_mod_2009

... – Where there is an A on the old strand, polymerase will add a T to the new strand. 3. In eukaryotic cells, replication process starts at the same time in several places along the DNA molecule. As the points of DNA replication meet each other, they combine and a new strand of DNA is formed (Fig. 8.3 ...
ppt link
ppt link

... replication (making 2 copies of the genome) before every cell division transcription: process of copying a portion of DNA gene sequence into a single stranded messenger RNA (mRNA) ...
1b Unit 6 DNA and RNA and protein synthesis notes
1b Unit 6 DNA and RNA and protein synthesis notes

... Step 2: mRNA is made from the DNA template  mRNA matches with free DNA nitrogen bases in a complimentary fashion  BASE PAIR RULE (DNA) A – U (RNA) (DNA) T – A (RNA) (DNA) G – C (RNA) (DNA) C – G (RNA) ...
Transcription
Transcription

... 2. Transcription – one of the DNA strands acts as a template for the production of RNA. Three different types of RNA are produced: messenger RNA (mRNA), ribosomal RNA (rRNA) and transfer RNA (tRNA). 3. Translation – when messenger RNA (mRNA) is read, the information encoded in the strand is translat ...
Lesson 3
Lesson 3

... Likewise, guanine and cytosine come together to form the G-C base pair. The bases are joined together by weak hydrogen bonds, and it is this hydrogen bonding that produces DNA's familiar double helix shape. Whatever nucleotides are in one strand, they rigidly fix the sequence of nucleotides in the o ...
BIO | DNA Review Worksheet | KEY
BIO | DNA Review Worksheet | KEY

... In area C, the ribosome is performing the process called translation. One by one, an amino acid is being delivered to the ribosome by the transfer RNA. 15. Examine area C. List the amino acids that will be delivered to this ribosome from start to finish. Remember, in order to find the amino acid, yo ...
Construct DNA Activity
Construct DNA Activity

... their daily jobs. Genes are short pieces of DNA that make up our chromosomes. Each piece, or strand, of DNA that is related to a gene makes up one trait. DNA looks like a twisted “ladder” made of chemical compounds called bases. There are 4 types of bases in DNA: Adenine, Guanine, Cytosine and Thymi ...
The Earth - Mr. Shanks` Class
The Earth - Mr. Shanks` Class

... 3’ carbon atom of one sugar molecule and the 5’ carbon atom of the next • Think: Phospho (phosphate) + diester (two esters) ...
DNA THE CODE OF LIFE 30 JANUARY 2013 Key Concepts
DNA THE CODE OF LIFE 30 JANUARY 2013 Key Concepts

... (Life Sciences for All, Chapter 4 DNA and the genetic code) There are two kinds of nucleic acids: deoxyribonucleic acid (DNA), ribonucleic acid (RNA). DNA is found in the nucleus of eukaryotic cells where it forms part of the chromatin. Some also found inside the mitochondria and chloroplasts of the ...
Making a DNA model - bendigoeducationplan
Making a DNA model - bendigoeducationplan

... cytosine (C) and they link together in pairs (A with T, C with G) to form a rung. The order of the bases and rungs creates a kind of code for the DNA information. WH AT DO E S DNA DO ? All living things contain DNA. Let’s work from the large to the small. Your body is made up of many different chemi ...
answer key
answer key

... 4. Using a ladder as an analogy, describe the structure of a double-stranded DNA molecule. Be sure to include the locations of the nitrogenous bases, sugar molecules, and phosphate groups in your description. In the ladder analogy, the sides of the ladder are made up of alternating phosphates and de ...
The Molecular Basis of Heredity
The Molecular Basis of Heredity

... sugar of another nucleotide. This bond is very strong, and for this reason DNA is remarkably stable. DNA can be boiled and even autoclaved without degrading! 5’ and 3’ ...
Sequencing User Guide - ASU Shared Resources
Sequencing User Guide - ASU Shared Resources

... Optimal amounts for templates and primers are as follows: ...
Translation
Translation

... Figure 16.7 A model for DNA replication: the basic concept (Layer 1) ...
Chapter 19 (part 1) - Nevada Agricultural Experiment
Chapter 19 (part 1) - Nevada Agricultural Experiment

... • First discovered in 1869 by Miescher. • Found as a precipitate that formed when extracts from nuclei were treated with acid. • Compound contained C, N, O, and high amount of P. • Was an acid compound found in nuclei therefore named nucleic acid ...
Organic molecules are the`molecules of life` which are contained in
Organic molecules are the`molecules of life` which are contained in

... Nucleic Acids are molecules that contain information for building proteins. The two kinds of nucleic acids are DNA (the actual blueprint) and RNA (a copy of DNA). All nucleic acids are made of building blocks called nucleotides. Each nucleotide has three parts - a 5-carbon sugar, a phosphate group, ...
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DNA replication



DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.
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